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P17809 (GTR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 2, facilitated glucose transporter member 1
Alternative name(s):
Glucose transporter type 1, erythrocyte/brain
Short name=GLUT-1
Short name=GT1
Gene names
Name:Slc2a1
Synonyms:Glut-1, Glut1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Facilitative glucose transporter. This isoform maybe responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. Ref.10

Subunit structure

Found in a complex with ADD2, DMTN and SLC2A1. Interacts (via C-terminus cytoplasmic region) with DMTN isoform 2 Interacts with SNX27; the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane. Interacts with GIPC (via PDZ domain). Interacts with STOM By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Melanosome By similarity. Note: Localizes primarily at the cell surface By similarity. Ref.10

Developmental stage

Levels decline 3-fold between days 7.5 and 12.5 of gestation. At 7.5 dpc, expressed more strongly in extraembryonic tissues than in the embryo proper. Expressed in amnion, chorion, and ectoplacental cone. In the yolk sac, expressed more strongly in the mesoderm layer than the ectoderm. Expression fairly widespread in the embryo at 8.5 dpc, but by 10.5 dpc, expression is down-regulated and observed in the eye and the spinal cord. Ref.8 Ref.9

Sequence similarities

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. [View classification]

Ontologies

Keywords
   Biological processSugar transport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMAcetylation
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to glucose starvation

Inferred from direct assay PubMed 11259273. Source: MGI

dehydroascorbic acid transport

Inferred from direct assay PubMed 18668520. Source: UniProtKB

glucose transport

Inferred from direct assay PubMed 18668520. Source: UniProtKB

protein complex assembly

Inferred from sequence or structural similarity. Source: UniProtKB

response to osmotic stress

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentbasolateral plasma membrane

Inferred from direct assay PubMed 11259273PubMed 15975910. Source: MGI

caveola

Inferred from electronic annotation. Source: Ensembl

cell-cell junction

Inferred from electronic annotation. Source: Ensembl

cortical actin cytoskeleton

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from direct assay PubMed 18668520. Source: UniProtKB

female pronucleus

Inferred from direct assay PubMed 11259273. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular

Inferred from direct assay PubMed 11172003. Source: MGI

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from direct assay PubMed 11259273. Source: MGI

midbody

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from direct assay PubMed 18668520. Source: UniProtKB

vesicle

Inferred from direct assay PubMed 11259273. Source: MGI

   Molecular_functionD-glucose transmembrane transporter activity

Inferred from direct assay PubMed 18668520. Source: UniProtKB

dehydroascorbic acid transporter activity

Inferred from direct assay PubMed 18668520. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 15975910. Source: MGI

protein self-association

Inferred from sequence or structural similarity. Source: UniProtKB

xenobiotic transporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Solute carrier family 2, facilitated glucose transporter member 1
PRO_0000050339

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3321Helical; Name=1; Potential
Topological domain34 – 6633Extracellular Potential
Transmembrane67 – 8721Helical; Name=2; Potential
Topological domain88 – 958Cytoplasmic Potential
Transmembrane96 – 11621Helical; Name=3; Potential
Topological domain117 – 12610Extracellular Potential
Transmembrane127 – 14721Helical; Name=4; Potential
Topological domain148 – 1558Cytoplasmic Potential
Transmembrane156 – 17621Helical; Name=5; Potential
Topological domain177 – 1859Extracellular Potential
Transmembrane186 – 20621Helical; Name=6; Potential
Topological domain207 – 27165Cytoplasmic Potential
Transmembrane272 – 29221Helical; Name=7; Potential
Topological domain293 – 30715Extracellular Potential
Transmembrane308 – 32821Helical; Name=8; Potential
Topological domain329 – 3379Cytoplasmic Potential
Transmembrane338 – 35821Helical; Name=9; Potential
Topological domain359 – 37113Extracellular Potential
Transmembrane372 – 39221Helical; Name=10; Potential
Topological domain393 – 4019Cytoplasmic Potential
Transmembrane402 – 42221Helical; Name=11; Potential
Topological domain423 – 4297Extracellular Potential
Transmembrane430 – 45021Helical; Name=12; Potential
Topological domain451 – 49242Cytoplasmic Potential
Region279 – 2813Defines substrate specificity By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue4781Phosphothreonine By similarity
Modified residue4901Phosphoserine By similarity
Glycosylation451N-linked (GlcNAc...) Ref.11 Ref.12
Glycosylation491N-linked (GlcNAc...); atypical Ref.11 Ref.12

Experimental info

Sequence conflict521Y → I in AAA37752. Ref.1
Sequence conflict193 – 1953IFI → VFV in AAA37752. Ref.1
Sequence conflict357 – 3604LLER → MQEQ in AAB20846. Ref.9
Sequence conflict4031A → R in AAA37752. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P17809 [UniParc].

Last modified July 27, 2011. Version 4.
Checksum: 464B668853D7636E

FASTA49253,985
        10         20         30         40         50         60 
MDPSSKKVTG RLMLAVGGAV LGSLQFGYNT GVINAPQKVI EEFYNQTWNH RYGEPIPSTT 

        70         80         90        100        110        120 
LTTLWSLSVA IFSVGGMIGS FSVGLFVNRF GRRNSMLMMN LLAFVAAVLM GFSKLGKSFE 

       130        140        150        160        170        180 
MLILGRFIIG VYCGLTTGFV PMYVGEVSPT ALRGALGTLH QLGIVVGILI AQVFGLDSIM 

       190        200        210        220        230        240 
GNADLWPLLL SVIFIPALLQ CILLPFCPES PRFLLINRNE ENRAKSVLKK LRGTADVTRD 

       250        260        270        280        290        300 
LQEMKEEGRQ MMREKKVTIL ELFRSPAYRQ PILIAVVLQL SQQLSGINAV FYYSTSIFEK 

       310        320        330        340        350        360 
AGVQQPVYAT IGSGIVNTAF TVVSLFVVER AGRRTLHLIG LAGMAGCAVL MTIALALLER 

       370        380        390        400        410        420 
LPWMSYLSIV AIFGFVAFFE VGPGPIPWFI VAELFSQGPR PAAIAVAGFS NWTSNFIVGM 

       430        440        450        460        470        480 
CFQYVEQLCG PYVFIIFTVL LVLFFIFTYF KVPETKGRTF DEIASGFRQG GASQSDKTPE 

       490 
ELFHPLGADS QV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence, tissue distribution, and differential expression of mRNA for a putative insulin-responsive glucose transporter in mouse 3T3-L1 adipocytes."
Kaestner K.H., Christy R.J., McLenithan J.C., Braiterman L.T., Cornelius P., Pekala P.H., Lane M.D.
Proc. Natl. Acad. Sci. U.S.A. 86:3150-3154(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"3T3-L1 adipocyte glucose transporter (HepG2 class): sequence and regulation of protein and mRNA expression by insulin, differentiation, and glucose starvation."
Reed B.C., Shade D., Alperovich F., Vang M.
Arch. Biochem. Biophys. 279:261-274(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NOD.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[7]"Identification of two enhancer elements in the gene encoding the type 1 glucose transporter from the mouse which are responsive to serum, growth factor, and oncogenes."
Murakami T., Nishiyama T., Shirotani T., Shinohara Y., Kan M., Ishii K., Kanai F., Nakazuru S., Ebina Y.
J. Biol. Chem. 267:9300-9306(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-6.
[8]"Differential screening of a PCR-generated mouse embryo cDNA library: glucose transporters are differentially expressed in early postimplantation mouse embryos."
Smith D.E., Gridley T.
Development 116:555-561(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 151-237, DEVELOPMENTAL STAGE.
Strain: C57BL/6.
Tissue: Embryo.
[9]"Glucose transporter gene expression in early mouse embryos."
Hogan A., Heyner S., Charron M.J., Copeland N.G., Gilbert D.J., Jenkins N.A., Thorens B., Schultz G.A.
Development 113:363-372(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 357-463, DEVELOPMENTAL STAGE.
Strain: CD-1.
Tissue: Embryo.
[10]"PACSIN3 overexpression increases adipocyte glucose transport through GLUT1."
Roach W., Plomann M.
Biochem. Biophys. Res. Commun. 355:745-750(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-45 AND ASN-49.
Tissue: Myoblast.
[12]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-45 AND ASN-49.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M23384 mRNA. Translation: AAA37752.1.
M22998 mRNA. Translation: AAA37707.1.
AK170873 mRNA. Translation: BAE42084.1.
AL606975 Genomic DNA. Translation: CAM21633.1.
CH466552 Genomic DNA. Translation: EDL30474.1.
BC055340 mRNA. Translation: AAH55340.1.
X69697 mRNA. Translation: CAA49367.1.
S77924 mRNA. Translation: AAB20846.2.
CCDSCCDS18569.1.
PIRA44887.
S09705.
RefSeqNP_035530.2. NM_011400.3.
UniGeneMm.21002.

3D structure databases

ProteinModelPortalP17809.
SMRP17809. Positions 19-465.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203304. 1 interaction.
IntActP17809. 2 interactions.
MINTMINT-348833.

PTM databases

PhosphoSiteP17809.

Proteomic databases

MaxQBP17809.
PaxDbP17809.
PRIDEP17809.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030398; ENSMUSP00000030398; ENSMUSG00000028645.
GeneID20525.
KEGGmmu:20525.
UCSCuc008ule.2. mouse.

Organism-specific databases

CTD6513.
MGIMGI:95755. Slc2a1.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00720000108560.
HOGENOMHOG000202871.
HOVERGENHBG014816.
InParanoidQ6GTI3.
KOK07299.
OMAQTWIHRY.
OrthoDBEOG7QVM2R.
TreeFamTF313762.

Gene expression databases

ArrayExpressP17809.
BgeeP17809.
GenevestigatorP17809.

Family and domain databases

InterProIPR002439. Glu_transpt_1.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSPR01190. GLUCTRSPORT1.
PR00171. SUGRTRNSPORT.
SUPFAMSSF103473. SSF103473. 2 hits.
TIGRFAMsTIGR00879. SP. 1 hit.
PROSITEPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC2A1. mouse.
NextBio298763.
PROP17809.
SOURCESearch...

Entry information

Entry nameGTR1_MOUSE
AccessionPrimary (citable) accession number: P17809
Secondary accession number(s): Q61608, Q6GTI3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 144 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot