P17789 (TTKB_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein tramtrack, beta isoform

Alternative name(s):
Repressor protein fushi tarazu
Tramtrack p69

Gene names

Name:	ttk
Synonyms:	FTZ-F2
ORF Names:	CG1856

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to a number of sites in the transcriptional regulatory region of ftz. Isoform beta is required to repress inappropriate segmentation gene transcription and repress genes incompatible with development of photoreceptor cell fates. Probable repressor of the transcription of the segmentation genes ftz, eve, h, odd, run, and en. Inhibits Trl-dependent activation of eve. May bind to the region AGGGC/TGG. Degradation of ttk is directed by binding of sinah or sina, via the adapter molecule phyl which binds to the BTB domain of ttk. A second method of degradation exists that is phyl-independent, this is mediated by recognition of motifs in the C-terminus of ttk. Ref.1 Ref.2 Ref.3 Ref.9 Ref.10
Subunit structure	Can form homodimers. Interacts with Trl in vivo via the BTB domain. Interacts with phyl. Ref.9 Ref.10
Subcellular location	Nucleus.
Developmental stage	Expressed both maternally and zygotically. Expressed in preblastoderm embryos, followed by complete decay upon formation of the cellular blastoderm when ftz striped expression is at its peak. Ref.1 Ref.2
Sequence similarities	Contains 1 BTB (POZ) domain. Contains 2 C2H2-type zinc fingers.
Caution	It is uncertain whether Met-1 or Met-3 is the initiator.
Sequence caution	The sequence AAA28544.1 differs from that shown. Reason: Erroneous initiation. The sequence ABB36443.1 differs from that shown. Reason: Frameshift at positions 83 and 426. The sequence CAA34981.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	R1/R6 development Inferred from mutant phenotype. Source: FlyBase R7 cell development Inferred from mutant phenotype. Source: FlyBase brain morphogenesis Inferred from mutant phenotype. Source: FlyBase branch fusion, open tracheal system Inferred from mutant phenotype. Source: FlyBase cell fate determination Traceable author statement. Source: FlyBase chitin-based cuticle development Inferred from mutant phenotype. Source: FlyBase compound eye cone cell differentiation Inferred from mutant phenotype. Source: FlyBase compound eye corneal lens morphogenesis Inferred from mutant phenotype. Source: FlyBase dendrite morphogenesis Inferred from mutant phenotype. Source: FlyBase dorsal appendage formation Inferred from mutant phenotype. Source: FlyBase dorsal trunk growth, open tracheal system Inferred from mutant phenotype. Source: FlyBase inter-male aggressive behavior Inferred from mutant phenotype. Source: FlyBase locomotion involved in locomotory behavior Inferred from mutant phenotype. Source: FlyBase negative regulation of transcription, DNA-dependent Inferred from direct assay. Source: FlyBase peripheral nervous system development Traceable author statement. Source: FlyBase regulation of embryonic cell shape Inferred from mutant phenotype. Source: FlyBase regulation of tube size, open tracheal system Inferred from mutant phenotype. Source: FlyBase startle response Inferred from mutant phenotype. Source: FlyBase tracheal outgrowth, open tracheal system Inferred from mutant phenotype. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay. Source: FlyBase polytene chromosome Inferred from direct assay. Source: FlyBase
Molecular function	RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred from direct assay. Source: FlyBase chromatin binding Inferred from direct assay. Source: FlyBase protein homodimerization activity Inferred from physical interaction Ref.9. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Beta (identifier: P17789-1)

Also known as: p69; C; D; F;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Alpha (identifier: P42282-1)

Also known as: p88; A; E;

The sequence of this isoform can be found in the external entry P42282.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 643

643

Protein tramtrack, beta isoform

PRO_0000047079

Regions

Domain

33 – 98

66

BTB

Zinc finger

508 – 531

24

C2H2-type 1

Zinc finger

538 – 561

24

C2H2-type 2

Experimental info

Sequence conflict

255

1

T → M in AAA28544. Ref.2

Sequence conflict

281

1

H → Q in AAA28544. Ref.2

Sequence conflict

501

1

T → I in AAA28544. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

643

Helix Strand Turn

Details...

Beta strand

501 – 504

4

Beta strand

507 – 509

3

Turn

511 – 513

3

Beta strand

516 – 519

4

Helix

520 – 530

11

Beta strand

531 – 534

4

Turn

541 – 543

3

Beta strand

546 – 548

3

Helix

550 – 560

11

Sequences

	Sequence		Length	Mass (Da)
	Isoform Beta (p69) (C) (D) (F) [UniParc]. Last modified May 2, 2002. Version 2. Checksum: 98DAEA1A5E97DC59	FASTA	643	68,771
	10 20 30 40 50 60 MKMASQRFCL RWNNHQSNLL SVFDQLLHAE TFTDVTLAVE GQHLKAHKMV LSACSPYFNT 70 80 90 100 110 120 LFVSHPEKHP IVILKDVPYS DMKSLLDFMY RGEVSVDQER LTAFLRVAES LRIKGLTEVN 130 140 150 160 170 180 DDKPSPAAAA AGAGATGSES TATTPQLQRI QPYLVPQRNR SQAGGLLASA ANAGNTPTLP 190 200 210 220 230 240 VQPSLLSSAL MPKRKRGRPR KLSGSSNGTG NDYDDFDREN MMNDSSDLGN GKMCNESYSG 250 260 270 280 290 300 NDDGSDDNQP NAGHTDDLNE SRDSLPSKRS KNSKDHRVVS HHEDNSTSDG NDSDGEGLDT 310 320 330 340 350 360 SYMEPQLMLD EYDEPVEFKY NPLTDNSSPT QDHTDGSHLN EQARQQAFLI AAQRKHQVET 370 380 390 400 410 420 AAAAAASGIK LNIIGMAAGG AQVKSMVSIP KLTPIGKVNA ASTPLVSPAG SFSTATVKPR 430 440 450 460 470 480 VQKRPKLGKQ NGDVKPAVFS SQEYLDIYNS NDGFKLKAAG LSGSTPNLSA GLGTPSVKTK 490 500 510 520 530 540 LNLSSNVGEG EAEGSVRDYC TKEGEHTYRC KVCSRVYTHI SNFCRHYVTS HKRNVKVYPC 550 560 570 580 590 600 PFCFKEFTRK DNMTAHVKII HKIENPSTAL ATVAAANLAG QPLGVSGAST PPPPDLSGQN 610 620 630 640 SNQSLPATSN ALSTSSSSST SSSSGSLGPL TTSAPPAPAA AAQ
	Isoform Alpha (p88) (A) (E) [UniParc]. See P42282.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The tramtrack gene encodes a Drosophila finger protein that interacts with the ftz transcriptional regulatory region and shows a novel embryonic expression pattern." Harrison S.D., Travers A.A. EMBO J. 9:207-216(1990) [PubMed: 2104801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE. Tissue: Embryo.
[2]	"Repression of Drosophila pair-rule segmentation genes by ectopic expression of tramtrack." Brown J.L., Wu C. Development 117:45-58(1993) [PubMed: 8223261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE. Tissue: Embryo.
[3]	"Tramtrack is a transcriptional repressor required for cell fate determination in the Drosophila eye." Xiong W.C., Montell C. Genes Dev. 7:1085-1096(1993) [PubMed: 8504931] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[4]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[5]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[6]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[7]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[8]	"Sequence-specific DNA binding by a two zinc-finger peptide from the Drosophila melanogaster Tramtrack protein." Fairall L., Harrison S.D., Travers A.A., Rhodes D. J. Mol. Biol. 226:349-366(1992) [PubMed: 1640455] [Abstract] Cited for: DNA-BINDING.
[9]	"The Drosophila transcription factor tramtrack (TTK) interacts with Trithorax-like (GAGA) and represses GAGA-mediated activation." Pagans S., Ortiz-Lombardia M., Espinas M.L., Bernues J., Azorin F. Nucleic Acids Res. 30:4406-4413(2002) [PubMed: 12384587] [Abstract] Cited for: FUNCTION, INTERACTION WITH TRL, HOMODIMERIZATION.
[10]	"Two modes of degradation of the tramtrack transcription factors by Siah homologues." Cooper S.E., Murawsky C.M., Lowe N., Travers A.A. J. Biol. Chem. 283:1076-1083(2008) [PubMed: 17962185] [Abstract] Cited for: FUNCTION, INTERACTION WITH PHYL.
[11]	"The crystal structure of a two zinc-finger peptide reveals an extension to the rules for zinc-finger/DNA recognition." Fairall L., Schwabe J.W.R., Chapman L., Finch J.T., Rhodes D. Nature 366:483-487(1993) [PubMed: 8247159] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 499-561.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X17121 mRNA. Translation: CAA34981.1. Different initiation.
M62856 mRNA. Translation: AAA28544.1. Different initiation.
X71627 mRNA. Translation: CAA50634.1.
AE014297 Genomic DNA. Translation: AAF57181.1.
AE014297 Genomic DNA. Translation: AAF57182.3.
AE014297 Genomic DNA. Translation: AAN14283.1.
AY122169 mRNA. Translation: AAM52681.1.
BT001723 mRNA. Translation: AAN71478.1.
BT023939 mRNA. Translation: ABB36443.1. Frameshift.

PIR

S36017.

RefSeq

NP_001189330.1. NM_001202401.1.
NP_524911.3. NM_080172.3.
NP_733446.1. NM_170567.2.
NP_733447.1. NM_170568.2.

UniGene

Dm.1526.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DRP	X-ray	2.80	A/D	501-563	[»]

ProteinModelPortal

P17789.

SMR

P17789. Positions 9-125, 501-562.

ModBase

Search...

Protein-protein interaction databases

STRING

P17789.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0085826; FBpp0085187; FBgn0003870.
FBtr0085828; FBpp0085189; FBgn0003870.
FBtr0085830; FBpp0085191; FBgn0003870.
FBtr0303228; FBpp0292320; FBgn0003870.

GeneID

48317.

KEGG

dme:Dmel_CG1856.

Organism-specific databases

CTD

7272.

FlyBase

FBgn0003870. ttk.

Phylogenomic databases

GeneTree

EMGT00050000002223.

InParanoid

P17789.

OMA

MLDEYDE.

Gene expression databases

ArrayExpress

P17789.

Bgee

P17789.

GermOnline

CG1856. Drosophila melanogaster.

Family and domain databases

InterPro

IPR017956. AT_hook_DNA-bd_motif.
IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

Gene3D

G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit.
G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 2 hits.

KO

K09237.

Pfam

PF00651. BTB. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]

SMART

SM00384. AT_hook. 1 hit.
SM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]

SUPFAM

SSF54695. BTB/POZ_fold. 1 hit.

PROSITE

PS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

839310.

Entry information

Entry name

TTKB_DROME

Accession

Primary (citable) accession number: P17789
Secondary accession number(s): A4V3Q4 , Q32KE0, Q9V9V1, Q9V9V3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	May 2, 2002
Last modified:	January 25, 2012
This is version 127 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project