Reviewed, UniProtKB/Swiss-Prot P17785 (ANXA2_CHICK)

Names and origin

Protein names

Recommended name:
    Annexin A2
Alternative name(s):
    Annexin-2
    Annexin II
    Lipocortin II
    Calpactin I heavy chain
    Chromobindin-8
    p36
    Protein I
    Placental anticoagulant protein IV
      Short name=PAP-IV

Gene names

Name:	ANXA2
Synonyms:	ANX2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.
Subunit structure	Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36.
Subcellular location	Secreted › extracellular space › extracellular matrix › basement membrane. Note: In the lamina beneath the plasma membrane.
Domain	A pair of annexin repeats may form one binding site for calcium and phospholipid.
Miscellaneous	It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Sequence similarities	Belongs to the annexin family. Contains 4 annexin repeats.

Ontologies

Keywords
Cellular component	Basement membrane Extracellular matrix Secreted
Domain	Annexin Repeat
Ligand	Calcium Calcium/phospholipid-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	basement membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-dependent phospholipid binding Inferred from electronic annotation. Source: UniProtKB-KW cytoskeletal protein binding Inferred from electronic annotation. Source: InterPro phospholipase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2 Ref.3 Ref.4

Chain

2 – 339

338

Annexin A2

PRO_0000067474

Regions

Repeat

42 – 102

61

Annexin 1

Repeat

114 – 174

61

Annexin 2

Repeat

199 – 259

61

Annexin 3

Repeat

274 – 334

61

Annexin 4

Region

2 – 24

23

S100A10-binding site Potential

Amino acid modifications

Modified residue

2

1

N-acetylserine Ref.3 Ref.4

Modified residue

24

1

Phosphotyrosine; by SRC By similarity

Modified residue

26

1

Phosphothreonine; by PKC By similarity

Secondary structure

1

.

.

.

339

Helix Strand Turn

Details...

Helix

3 – 11

9

Sequences

	Sequence		Length	Mass (Da)
	P17785-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 4B621506C4BFCD73	FASTA	339	38,640
	10 20 30 40 50 60 MSTVHEILSK LSLEGDHSLP PSAYATVKAY SNFDADRDAA ALEAAIKTKG VDEVTIINIL 70 80 90 100 110 120 TNRSNEQRQD IAFAYQRRTK KELSAALKSA LSGHLEAVIL GLLKTPSQYD ASELKAAMKG 130 140 150 160 170 180 LGTDEDTLIE IICSRTNQEL NEINRVYREM YKTELEKDII SDTSGDFRKL MVALAKGKRC 190 200 210 220 230 240 EDTSVIDYEL IDQDARELYD AGVKRKGTDV PKWINIMTER SVPHLQKVFE RYKSYSPYDM 250 260 270 280 290 300 LESIKKEVKG DLENAFLNLV QCIQNKQLYF ADRLYDSMKG KGTRDKVLIR IMVSRCEVDM 310 320 330 LKIKSEFKRK YGKSLYYFIQ QDTKGDYQRA LLNLCGGED

References

[1]	"The cDNA sequence of chicken annexin II." Gerke V., Koch W. Nucleic Acids Res. 18:4246-4246(1990) [PubMed: 2143014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A discontinuous epitope on p36, the major substrate of src tyrosine-protein-kinase, brings the phosphorylation site into the neighbourhood of a consensus sequence for Ca2+/lipid-binding proteins." Johnsson N., Johnsson K., Weber K. FEBS Lett. 236:201-204(1988) [PubMed: 2456953] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-70.
[3]	"p36, the major cytoplasmic substrate of src tyrosine protein kinase, binds to its p11 regulatory subunit via a short amino-terminal amphiphatic helix." Johnsson N., Marriott G., Weber K. EMBO J. 7:2435-2442(1988) [PubMed: 2973411] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-30, ACETYLATION AT SER-2.
[4]	Bienvenut W.V., Black E.J., Gillespie D.A. Submitted (JAN-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-63; 69-77 AND 314-324, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Red velvet - Issue 86 of September 2007

Cross-references

Sequence databases

EMBLGenBankDDBJ

X53334 mRNA. Translation: CAA37421.1.

IPI

IPI00577039.

PIR

LUCH2. S10501.

RefSeq

NP_990682.1.

UniGene

Gga.3641

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BT6	X-ray	2.40	C/D	2-14	[»]

SMR

P17785. Positions 21-339.

ModBase

Search...

Protein-protein interaction databases

STRING

P17785.

Genome annotation databases

Ensembl

ENSGALT00000005981; ENSGALP00000005971; ENSGALG00000003770; Gallus gallus. [Genome view]
ENSGALT00000039485; ENSGALP00000038695; ENSGALG00000003770; Gallus gallus. [Genome view]

GeneID

396297.

KEGG

gga:396297.

Organism-specific databases

CTD

396297.

Phylogenomic databases

HOGENOM

P17785.

HOVERGEN

P17785.

OrthoDB

EOG9B2WHD

Family and domain databases

InterPro

IPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]

Gene3D

G3DSA:1.10.220.10. Annexin. 4 hits.

PANTHER

PTHR10502. Annexin. 1 hit.
PTHR10502:SF18. AnnexinII. 1 hit.

Pfam

PF00191. Annexin. 4 hits.
[Graphical view]

PRINTS

PR00196. ANNEXIN.
PR00198. ANNEXINII.

SMART

SM00335. ANX. 4 hits.
[Graphical view]

PROSITE

PS00223. ANNEXIN. 4 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ANXA2_CHICK

Accession

Primary (citable) accession number: P17785

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)