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P17784 (ALF_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-bisphosphate aldolase cytoplasmic isozyme

EC=4.1.2.13
Alternative name(s):
Gravity-specific protein GSC 233
Gene names
Name:FBA
Ordered Locus Names:Os05g0402700, LOC_Os05g33380
ORF Names:OSJNBa0035J16.18, OSJNBb0006J12.6
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Subcellular location

Cytoplasm.

Tissue specificity

Expressed in callus. Ref.4

Induction

By gravity stress. Ref.4

Sequence similarities

Belongs to the class I fructose-bisphosphate aldolase family.

Sequence caution

The sequence AAB25853.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

The sequence AAB25853.1 differs from that shown. Reason: Frameshift at several positions.

The sequence AAB25853.1 differs from that shown. Reason: Sequencing errors.

The sequence BAA01911.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

The sequence BAA01911.1 differs from that shown. Reason: Frameshift at several positions.

The sequence BAA01911.1 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
   Biological processGlycolysis
Stress response
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from sequence or structural similarity Ref.1. Source: Gramene

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity Ref.1. Source: Gramene

cytosol

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from sequence or structural similarity Ref.1. Source: Gramene

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 358358Fructose-bisphosphate aldolase cytoplasmic isozyme
PRO_0000216922

Sites

Active site1831Proton acceptor By similarity
Active site2251Schiff-base intermediate with dihydroxyacetone-P
Binding site521Substrate
Binding site1421Substrate
Site3581Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate

Experimental info

Sequence conflict1601D → H in CAA37290. Ref.1
Sequence conflict1961R → P in CAA37290. Ref.1
Sequence conflict2711E → K in CAA37290. Ref.1
Sequence conflict2761L → R in CAA37290. Ref.1
Sequence conflict3131E → A in CAA37290. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P17784 [UniParc].

Last modified October 25, 2005. Version 2.
Checksum: 2C5BBE05DD1AA83E

FASTA35838,863
        10         20         30         40         50         60 
MSAYCGKYKD ELIKNAAYIG TPGKGILAAD ESTGTIGKRF ASINVENVEE NRRSLRELLF 

        70         80         90        100        110        120 
CTPGALQYLS GVILFEETLY QKTKDGKPFV DVLKEGGVLP GIKVDKGTIE VAGTEKETTT 

       130        140        150        160        170        180 
QGHDDLGKRC AKYYEAGARF AKWRAVLKIG PNEPSQLAID LNAQGLARYA IICQENGLVP 

       190        200        210        220        230        240 
IVEPEILVDG PHDIDRCAYV SEVVLAACYK ALNEHHVLLE GTLLKPNMVT PGSDAKKVSP 

       250        260        270        280        290        300 
EVIAEYTVRT LQRTVPAAVP AIVFLSGGQS EEEATLNLNA MNKLSTKKPW SLSFSFGRAL 

       310        320        330        340        350 
QQSTLKAWSG KAENIEKARA AFLTRCKANS EATLGTYKGD AVLGEGASES LHVKDYKY 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the rice cytoplasmic aldolase cDNA."
Hidaka S., Kadowaki K., Tsutsumi K., Ishikawa K.
Nucleic Acids Res. 18:3991-3991(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Nipponbare.
[2]"Nucleotide sequence of fructose 1,6-bisphosphate aldolase (FBA) of Oryza sativa (Tainung No.71)."
Huang T.-C., Chuang H.-S., Yen T.-Y., Huang Y.-W., Wu M.-L.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Tainung 71.
[3]"A fine physical map of the rice chromosome 5."
Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.
Mol. Genet. Genomics 274:337-345(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"Molecular cloning and characterization of gravity specific cDNA in rice (Oryza sativa L.) suspension callus."
Kwon S., Kikuchi S., Oono K.
Jpn. J. Genet. 67:335-348(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 222-358, TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Nipponbare.
Tissue: Callus.
[5]"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 274-283.
Strain: cv. Nipponbare.
Tissue: Stem.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53130 mRNA. Translation: CAA37290.1.
AY522925 mRNA. Translation: AAS05825.1.
AC120991 Genomic DNA. Translation: AAT85207.1.
AC135418 Genomic DNA. Translation: AAT85154.1.
D11137 mRNA. Translation: BAA01911.1. Sequence problems.
S56877 mRNA. Translation: AAB25853.1. Sequence problems.
PIRADRZY. JQ0543.
T04310.

3D structure databases

ProteinModelPortalP17784.
SMRP17784. Positions 9-358.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP17784.
PRIDEP17784.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP17784.

Phylogenomic databases

eggNOGCOG3588.
HOGENOMHOG000220876.
OMADMEHCQY.

Enzyme and pathway databases

UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERPTHR11627. PTHR11627. 1 hit.
PfamPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALF_ORYSJ
AccessionPrimary (citable) accession number: P17784
Secondary accession number(s): Q07077, Q6QWQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 25, 2005
Last modified: April 16, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways