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Protein

Fructose-bisphosphate aldolase cytoplasmic isozyme

Gene

FBA

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521Substrate
Binding sitei142 – 1421Substrate
Active sitei183 – 1831Proton acceptorBy similarity
Active sitei225 – 2251Schiff-base intermediate with dihydroxyacetone-P
Sitei358 – 3581Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: Gramene

GO - Biological processi

  1. glycolytic process Source: Gramene
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis, Stress response

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

ReactomeiREACT_243951. Glycolysis.
REACT_254676. Gluconeogenesis.
REACT_262849. Fructose catabolism.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase cytoplasmic isozyme (EC:4.1.2.13)
Alternative name(s):
Gravity-specific protein GSC 233
Gene namesi
Name:FBA
Ordered Locus Names:Os05g0402700, LOC_Os05g33380
ORF Names:OSJNBa0035J16.18, OSJNBb0006J12.6
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 5

Organism-specific databases

GrameneiP17784.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Gramene
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Fructose-bisphosphate aldolase cytoplasmic isozymePRO_0000216922Add
BLAST

Proteomic databases

PaxDbiP17784.
PRIDEiP17784.

Expressioni

Tissue specificityi

Expressed in callus.1 Publication

Inductioni

By gravity stress.1 Publication

Gene expression databases

ExpressionAtlasiP17784. baseline and differential.

Structurei

3D structure databases

ProteinModelPortaliP17784.
SMRiP17784. Positions 9-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3588.
HOGENOMiHOG000220876.
InParanoidiP17784.
OMAiCAYVTER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAYCGKYKD ELIKNAAYIG TPGKGILAAD ESTGTIGKRF ASINVENVEE
60 70 80 90 100
NRRSLRELLF CTPGALQYLS GVILFEETLY QKTKDGKPFV DVLKEGGVLP
110 120 130 140 150
GIKVDKGTIE VAGTEKETTT QGHDDLGKRC AKYYEAGARF AKWRAVLKIG
160 170 180 190 200
PNEPSQLAID LNAQGLARYA IICQENGLVP IVEPEILVDG PHDIDRCAYV
210 220 230 240 250
SEVVLAACYK ALNEHHVLLE GTLLKPNMVT PGSDAKKVSP EVIAEYTVRT
260 270 280 290 300
LQRTVPAAVP AIVFLSGGQS EEEATLNLNA MNKLSTKKPW SLSFSFGRAL
310 320 330 340 350
QQSTLKAWSG KAENIEKARA AFLTRCKANS EATLGTYKGD AVLGEGASES

LHVKDYKY
Length:358
Mass (Da):38,863
Last modified:October 25, 2005 - v2
Checksum:i2C5BBE05DD1AA83E
GO

Sequence cautioni

The sequence AAB25853.1 differs from that shown.Sequencing errors.Curated
The sequence AAB25853.1 differs from that shown. Reason: Frameshift at several positions. Curated
The sequence BAA01911.1 differs from that shown.Sequencing errors.Curated
The sequence BAA01911.1 differs from that shown. Reason: Frameshift at several positions. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1601D → H in CAA37290 (PubMed:2374721).Curated
Sequence conflicti196 – 1961R → P in CAA37290 (PubMed:2374721).Curated
Sequence conflicti271 – 2711E → K in CAA37290 (PubMed:2374721).Curated
Sequence conflicti276 – 2761L → R in CAA37290 (PubMed:2374721).Curated
Sequence conflicti313 – 3131E → A in CAA37290 (PubMed:2374721).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53130 mRNA. Translation: CAA37290.1.
AY522925 mRNA. Translation: AAS05825.1.
AC120991 Genomic DNA. Translation: AAT85207.1.
AC135418 Genomic DNA. Translation: AAT85154.1.
D11137 mRNA. Translation: BAA01911.1. Sequence problems.
S56877 mRNA. Translation: AAB25853.1. Sequence problems.
PIRiJQ0543. ADRZY.
T04310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53130 mRNA. Translation: CAA37290.1.
AY522925 mRNA. Translation: AAS05825.1.
AC120991 Genomic DNA. Translation: AAT85207.1.
AC135418 Genomic DNA. Translation: AAT85154.1.
D11137 mRNA. Translation: BAA01911.1. Sequence problems.
S56877 mRNA. Translation: AAB25853.1. Sequence problems.
PIRiJQ0543. ADRZY.
T04310.

3D structure databases

ProteinModelPortaliP17784.
SMRiP17784. Positions 9-358.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP17784.
PRIDEiP17784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiP17784.

Phylogenomic databases

eggNOGiCOG3588.
HOGENOMiHOG000220876.
InParanoidiP17784.
OMAiCAYVTER.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
ReactomeiREACT_243951. Glycolysis.
REACT_254676. Gluconeogenesis.
REACT_262849. Fructose catabolism.

Gene expression databases

ExpressionAtlasiP17784. baseline and differential.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the rice cytoplasmic aldolase cDNA."
    Hidaka S., Kadowaki K., Tsutsumi K., Ishikawa K.
    Nucleic Acids Res. 18:3991-3991(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
  2. "Nucleotide sequence of fructose 1,6-bisphosphate aldolase (FBA) of Oryza sativa (Tainung No.71)."
    Huang T.-C., Chuang H.-S., Yen T.-Y., Huang Y.-W., Wu M.-L.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Tainung 71.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "Molecular cloning and characterization of gravity specific cDNA in rice (Oryza sativa L.) suspension callus."
    Kwon S., Kikuchi S., Oono K.
    Jpn. J. Genet. 67:335-348(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 222-358, TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Nipponbare.
    Tissue: Callus.
  5. "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
    Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
    Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 274-283.
    Strain: cv. Nipponbare.
    Tissue: Stem.

Entry informationi

Entry nameiALF_ORYSJ
AccessioniPrimary (citable) accession number: P17784
Secondary accession number(s): Q07077, Q6QWQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 25, 2005
Last modified: January 7, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.