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P17784

- ALF_ORYSJ

UniProt

P17784 - ALF_ORYSJ

Protein

Fructose-bisphosphate aldolase cytoplasmic isozyme

Gene

FBA

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (25 Oct 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521Substrate
    Binding sitei142 – 1421Substrate
    Active sitei183 – 1831Proton acceptorBy similarity
    Active sitei225 – 2251Schiff-base intermediate with dihydroxyacetone-P
    Sitei358 – 3581Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: Gramene

    GO - Biological processi

    1. glycolytic process Source: Gramene
    2. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis, Stress response

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase cytoplasmic isozyme (EC:4.1.2.13)
    Alternative name(s):
    Gravity-specific protein GSC 233
    Gene namesi
    Name:FBA
    Ordered Locus Names:Os05g0402700, LOC_Os05g33380
    ORF Names:OSJNBa0035J16.18, OSJNBb0006J12.6
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 5

    Organism-specific databases

    GrameneiP17784.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Gramene
    2. cytosol Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Fructose-bisphosphate aldolase cytoplasmic isozymePRO_0000216922Add
    BLAST

    Proteomic databases

    PaxDbiP17784.
    PRIDEiP17784.

    Expressioni

    Tissue specificityi

    Expressed in callus.1 Publication

    Inductioni

    By gravity stress.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP17784.
    SMRiP17784. Positions 9-358.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3588.
    HOGENOMiHOG000220876.
    OMAiDMEHCQY.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view]
    PfamiPF00274. Glycolytic. 1 hit.
    [Graphical view]
    PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17784-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAYCGKYKD ELIKNAAYIG TPGKGILAAD ESTGTIGKRF ASINVENVEE    50
    NRRSLRELLF CTPGALQYLS GVILFEETLY QKTKDGKPFV DVLKEGGVLP 100
    GIKVDKGTIE VAGTEKETTT QGHDDLGKRC AKYYEAGARF AKWRAVLKIG 150
    PNEPSQLAID LNAQGLARYA IICQENGLVP IVEPEILVDG PHDIDRCAYV 200
    SEVVLAACYK ALNEHHVLLE GTLLKPNMVT PGSDAKKVSP EVIAEYTVRT 250
    LQRTVPAAVP AIVFLSGGQS EEEATLNLNA MNKLSTKKPW SLSFSFGRAL 300
    QQSTLKAWSG KAENIEKARA AFLTRCKANS EATLGTYKGD AVLGEGASES 350
    LHVKDYKY 358
    Length:358
    Mass (Da):38,863
    Last modified:October 25, 2005 - v2
    Checksum:i2C5BBE05DD1AA83E
    GO

    Sequence cautioni

    The sequence AAB25853.1 differs from that shown. Reason: Sequencing errors.
    The sequence BAA01911.1 differs from that shown. Reason: Sequencing errors.
    The sequence AAB25853.1 differs from that shown. Reason: Frameshift at several positions.
    The sequence BAA01911.1 differs from that shown. Reason: Frameshift at several positions.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti160 – 1601D → H in CAA37290. (PubMed:2374721)Curated
    Sequence conflicti196 – 1961R → P in CAA37290. (PubMed:2374721)Curated
    Sequence conflicti271 – 2711E → K in CAA37290. (PubMed:2374721)Curated
    Sequence conflicti276 – 2761L → R in CAA37290. (PubMed:2374721)Curated
    Sequence conflicti313 – 3131E → A in CAA37290. (PubMed:2374721)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53130 mRNA. Translation: CAA37290.1.
    AY522925 mRNA. Translation: AAS05825.1.
    AC120991 Genomic DNA. Translation: AAT85207.1.
    AC135418 Genomic DNA. Translation: AAT85154.1.
    D11137 mRNA. Translation: BAA01911.1. Sequence problems.
    S56877 mRNA. Translation: AAB25853.1. Sequence problems.
    PIRiJQ0543. ADRZY.
    T04310.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53130 mRNA. Translation: CAA37290.1 .
    AY522925 mRNA. Translation: AAS05825.1 .
    AC120991 Genomic DNA. Translation: AAT85207.1 .
    AC135418 Genomic DNA. Translation: AAT85154.1 .
    D11137 mRNA. Translation: BAA01911.1 . Sequence problems.
    S56877 mRNA. Translation: AAB25853.1 . Sequence problems.
    PIRi JQ0543. ADRZY.
    T04310.

    3D structure databases

    ProteinModelPortali P17784.
    SMRi P17784. Positions 9-358.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P17784.
    PRIDEi P17784.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P17784.

    Phylogenomic databases

    eggNOGi COG3588.
    HOGENOMi HOG000220876.
    OMAi DMEHCQY.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view ]
    Pfami PF00274. Glycolytic. 1 hit.
    [Graphical view ]
    PROSITEi PS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the rice cytoplasmic aldolase cDNA."
      Hidaka S., Kadowaki K., Tsutsumi K., Ishikawa K.
      Nucleic Acids Res. 18:3991-3991(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Nipponbare.
    2. "Nucleotide sequence of fructose 1,6-bisphosphate aldolase (FBA) of Oryza sativa (Tainung No.71)."
      Huang T.-C., Chuang H.-S., Yen T.-Y., Huang Y.-W., Wu M.-L.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Tainung 71.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Molecular cloning and characterization of gravity specific cDNA in rice (Oryza sativa L.) suspension callus."
      Kwon S., Kikuchi S., Oono K.
      Jpn. J. Genet. 67:335-348(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 222-358, TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Nipponbare.
      Tissue: Callus.
    5. "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
      Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
      Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 274-283.
      Strain: cv. Nipponbare.
      Tissue: Stem.

    Entry informationi

    Entry nameiALF_ORYSJ
    AccessioniPrimary (citable) accession number: P17784
    Secondary accession number(s): Q07077, Q6QWQ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: October 25, 2005
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3