Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Malate dehydrogenase, mitochondrial

Gene

MMDH

Organism
Citrullus lanatus (Watermelon) (Citrullus vulgaris)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671NADBy similarity
Binding sitei114 – 1141SubstratePROSITE-ProRule annotation
Binding sitei120 – 1201SubstratePROSITE-ProRule annotation
Binding sitei127 – 1271NADBy similarity
Binding sitei152 – 1521SubstratePROSITE-ProRule annotation
Binding sitei186 – 1861SubstratePROSITE-ProRule annotation
Active sitei210 – 2101Proton acceptorBy similarity
Binding sitei261 – 2611NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi41 – 477NADBy similarity
Nucleotide bindingi150 – 1523NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, mitochondrial (EC:1.1.1.37)
Gene namesi
Name:MMDH
OrganismiCitrullus lanatus (Watermelon) (Citrullus vulgaris)
Taxonomic identifieri3654 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCitrullus

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Protein family/group databases

Allergomei6159. Cit la MDH.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727Mitochondrion1 PublicationAdd
BLAST
Chaini28 – 347320Malate dehydrogenase, mitochondrialPRO_0000018625Add
BLAST

Proteomic databases

PRIDEiP17783.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP17783.
SMRiP17783. Positions 36-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17783-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKASILRSVR SAVSRSSSSN RLLSRSFATE SVPERKVAVL GAAGGIGQPL
60 70 80 90 100
ALLMKLNPLV SKLALYDIAG TPGVAADVGH VNTRSEVTGY VGEEQLGKAL
110 120 130 140 150
EGSDVVIIPA GVPRKPGMTR DDLFNINAGI VKSLCTAIAK YCPNALINMI
160 170 180 190 200
SNPVNSTVPI AAEVFKKAGT YDEKKLFGVT TLDVVRAKTF YAGKANVPVA
210 220 230 240 250
EVNVPVIGGH AGITILPLFS QATPRANLSD DTIVALTKRT QDGGTEVVEA
260 270 280 290 300
KAGKGSATLS MAYAGALFAD ACLKGLNGVP DVVECSFVQS TVTELPFFAS
310 320 330 340
KVKLGKNGVE SVLDLGPLSD FEKEGLEKLK PELKASIEKG IQFANAN
Length:347
Mass (Da):36,201
Last modified:August 1, 1990 - v1
Checksum:i31474F15018D30CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17362 mRNA. Translation: CAA35239.1.
PIRiS10162. DEPUMW.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17362 mRNA. Translation: CAA35239.1.
PIRiS10162. DEPUMW.

3D structure databases

ProteinModelPortaliP17783.
SMRiP17783. Positions 36-343.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei6159. Cit la MDH.

Proteomic databases

PRIDEiP17783.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mitochondrial malate dehydrogenase from watermelon: sequence of cDNA clones and primary structure of the higher-plant precursor protein."
    Gietl C., Lehnerer M., Olsen O.
    Plant Mol. Biol. 14:1019-1030(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Sugar Baby.
    Tissue: Cotyledon.
  2. "Sequence homologies between glyoxysomal and mitochondrial malate dehydrogenase."
    Gietl C., Lottspeich F., Hock B.
    Planta 169:555-558(1986)
    Cited for: PROTEIN SEQUENCE OF 28-55.

Entry informationi

Entry nameiMDHM_CITLA
AccessioniPrimary (citable) accession number: P17783
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: January 7, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.