P17760 (VPX_HV2D1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Vpx

Alternative name(s):
Viral protein X
X ORF protein

Gene names

Name:

vpx

Organism

Human immunodeficiency virus type 2 subtype A (isolate D194) (HIV-2) [Complete proteome]

Taxonomic identifier

11713 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Lentivirus › Primate lentivirus group › Human immunodeficiency virus 2

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription By similarity.
Subunit structure	Interacts with the P6 region of unprocessed GAG. Interacts with host VPRBP/DCAF1, leading to change substrate specificity of the CUL4A-DDB1 E3 ligase complex By similarity.
Subcellular location	Virion. Host nucleus. Note: Nuclear just after virion uncoating, or if expressed in the absence of unprocessed GAG By similarity.
Miscellaneous	This isolate is from a Gambian case of 'neuro-AIDS'.
Sequence similarities	Belongs to the lentivirus VPX protein family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host innate immune response by virus Viral immunoevasion
Cellular component	Host nucleus Virion
Disease	AIDS
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 112	112	Protein Vpx		PRO_0000085389
Regions
	Motif	65 – 72	8	Nuclear localization signal By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17760 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 747AC8D908EA66B4	FASTA	112	12,820
	10 20 30 40 50 60 MADPRERVPP GNSGEETIGE AFEWLDRTIE ALNREAVNHL PRELIFQVWQ RSWAYWHDEQ 70 80 90 100 110 GMSTSYTKYR YLCIMQKAVY IHFKKGCTCL GRGHGPGGWR PGPPPPPPPG LV

References

[1]

"Molecular cloning of two west African human immunodeficiency virus type 2 isolates that replicate well in macrophages: a Gambian isolate, from a patient with neurologic acquired immunodeficiency syndrome, and a highly divergent Ghanian isolate." Kuehnel H., von Briesen H., Dietrich U., Adamski M., Mix D., Biesert L., Kreutz R., Immelmann A., Henco K., Meichsner C., Andreesen R., Gelderblom H., Ruebsamen-Waigmann H. Proc. Natl. Acad. Sci. U.S.A. 86:2383-2387(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

[2]

"Nucleotide sequence of HIV-2D194, an isolate from a Gambian case of 'neuro-AIDS', which showed excellent growth in macrophages." Kuehnel H., Kreutz R., Ruebsamen-Waigmann H. Nucleic Acids Res. 18:6142-6142(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04542 Genomic DNA. Translation: AAA76843.1. X52223 Genomic DNA. Translation: CAA36467.1.
PIR	S12155.
3D structure databases
ProteinModelPortal	P17760.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000012. RetroV_VpR/X. [Graphical view]
Pfam	PF00522. VPR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VPX_HV2D1

Accession

Primary (citable) accession number: P17760

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program