##gff-version 3 P17751 UniProtKB Initiator methionine 1 1 . . . Note=Removed;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P60174 P17751 UniProtKB Chain 2 249 . . . ID=PRO_0000090116;Note=Triosephosphate isomerase P17751 UniProtKB Active site 96 96 . . . Note=Electrophile;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU10127 P17751 UniProtKB Active site 166 166 . . . Note=Proton acceptor;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU10127 P17751 UniProtKB Binding site 12 12 . . . Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU10127 P17751 UniProtKB Binding site 14 14 . . . Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU10127 P17751 UniProtKB Modified residue 14 14 . . . Note=N6-acetyllysine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P60174 P17751 UniProtKB Modified residue 68 68 . . . Note=3'-nitrotyrosine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:16800626;Dbxref=PMID:16800626 P17751 UniProtKB Modified residue 80 80 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P60174 P17751 UniProtKB Modified residue 106 106 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P48500 P17751 UniProtKB Modified residue 149 149 . . . Note=N6-succinyllysine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:23806337;Dbxref=PMID:23806337 P17751 UniProtKB Modified residue 156 156 . . . Note=N6-acetyllysine%3B alternate;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:23806337;Dbxref=PMID:23806337 P17751 UniProtKB Modified residue 156 156 . . . Note=N6-succinyllysine%3B alternate;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:23806337;Dbxref=PMID:23806337 P17751 UniProtKB Modified residue 159 159 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:21183079;Dbxref=PMID:21183079 P17751 UniProtKB Modified residue 173 173 . . . Note=Phosphothreonine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:21183079;Dbxref=PMID:21183079 P17751 UniProtKB Modified residue 194 194 . . . Note=N6-acetyllysine%3B alternate;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:23806337;Dbxref=PMID:23806337 P17751 UniProtKB Modified residue 194 194 . . . Note=N6-methyllysine%3B alternate;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P60174 P17751 UniProtKB Modified residue 194 194 . . . Note=N6-succinyllysine%3B alternate;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:23806337;Dbxref=PMID:23806337 P17751 UniProtKB Modified residue 209 209 . . . Note=3'-nitrotyrosine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:16800626;Dbxref=PMID:16800626 P17751 UniProtKB Modified residue 212 212 . . . Note=Phosphoserine;Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:17242355,ECO:0007744|PubMed:21183079;Dbxref=PMID:17242355,PMID:21183079 P17751 UniProtKB Modified residue 214 214 . . . Note=Phosphothreonine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P60174 P17751 UniProtKB Modified residue 223 223 . . . Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P60174 P17751 UniProtKB Modified residue 238 238 . . . Note=N6-acetyllysine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P60174 P17751 UniProtKB Cross-link 142 142 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1);Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P60174 P17751 UniProtKB Alternative sequence 1 1 . . . ID=VSP_060724;Note=In isoform 2. M->MEGKAEQQGAGLTMAEGGEKEEFCFTAIYISGQWREPCVCTDLQRLEPGTM;Ontology_term=ECO:0000305;evidence=ECO:0000305 P17751 UniProtKB Mutagenesis 163 163 . . . Note=50%25 reduced activity. L->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7739600;Dbxref=PMID:7739600 P17751 UniProtKB Mutagenesis 193 193 . . . Note=50%25 reduced activity. L->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7739600;Dbxref=PMID:7739600 P17751 UniProtKB Sequence conflict 74 74 . . . Note=A->P;Ontology_term=ECO:0000305;evidence=ECO:0000305 P17751 UniProtKB Sequence conflict 107 107 . . . Note=D->G;Ontology_term=ECO:0000305;evidence=ECO:0000305 P17751 UniProtKB Sequence conflict 194 194 . . . Note=K->R;Ontology_term=ECO:0000305;evidence=ECO:0000305 P17751 UniProtKB Sequence conflict 195 195 . . . Note=S->P;Ontology_term=ECO:0000305;evidence=ECO:0000305 P17751 UniProtKB Sequence conflict 216 216 . . . Note=A->G;Ontology_term=ECO:0000305;evidence=ECO:0000305 P17751 UniProtKB Sequence conflict 222 222 . . . Note=A->Q;Ontology_term=ECO:0000305;evidence=ECO:0000305 P17751 UniProtKB Sequence conflict 223 225 . . . Note=SQP->TPA;Ontology_term=ECO:0000305;evidence=ECO:0000305 P17751 UniProtKB Sequence conflict 227 227 . . . Note=V->M;Ontology_term=ECO:0000305;evidence=ECO:0000305