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Protein

Triosephosphate isomerase

Gene

Tpi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Caution

It is uncertain if Met-1 or Met-14 is the initiator.Curated

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (Tpi1), Triosephosphate isomerase (Tpi1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62SubstrateBy similarity1
Binding sitei64SubstrateBy similarity1
Active sitei146ElectrophileBy similarity1
Active sitei216Proton acceptorBy similarity1

GO - Molecular functioni

  • isomerase activity Source: MGI
  • triose-phosphate isomerase activity Source: MGI
  • ubiquitin protein ligase binding Source: MGI

GO - Biological processi

  • gluconeogenesis Source: GO_Central
  • glucose metabolic process Source: MGI
  • glyceraldehyde-3-phosphate biosynthetic process Source: GO_Central
  • glyceraldehyde-3-phosphate metabolic process Source: UniProtKB
  • glycerol catabolic process Source: GO_Central
  • glycolytic process Source: GO_Central
  • multicellular organism development Source: MGI

Keywordsi

Molecular functionIsomerase
Biological processGluconeogenesis, Glycolysis

Enzyme and pathway databases

ReactomeiR-MMU-70171 Glycolysis
R-MMU-70263 Gluconeogenesis
SABIO-RKiP17751
UniPathwayiUPA00109; UER00189
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase (EC:5.3.1.1)
Short name:
TIM
Alternative name(s):
Triose-phosphate isomerase
Gene namesi
Name:Tpi1
Synonyms:Tpi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:98797 Tpi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi213L → Q: 50% reduced activity. 1 Publication1
Mutagenesisi243L → Q: 50% reduced activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000901161 – 299Triosephosphate isomeraseAdd BLAST299

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64N6-acetyllysineBy similarity1
Modified residuei118Nitrated tyrosineCombined sources1
Modified residuei130PhosphoserineBy similarity1
Modified residuei156PhosphoserineBy similarity1
Cross-linki192Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei199N6-succinyllysineCombined sources1
Modified residuei206N6-acetyllysine; alternateCombined sources1
Modified residuei206N6-succinyllysine; alternateCombined sources1
Modified residuei209PhosphoserineCombined sources1
Modified residuei223PhosphothreonineCombined sources1
Modified residuei244N6-acetyllysine; alternateCombined sources1
Modified residuei244N6-methyllysine; alternateBy similarity1
Modified residuei244N6-succinyllysine; alternateCombined sources1
Modified residuei259Nitrated tyrosineCombined sources1
Modified residuei262PhosphoserineCombined sources1
Modified residuei264PhosphothreonineBy similarity1
Modified residuei273PhosphoserineBy similarity1
Modified residuei288N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Nitration, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP17751
MaxQBiP17751
PaxDbiP17751
PeptideAtlasiP17751
PRIDEiP17751
TopDownProteomicsiP17751

2D gel databases

COMPLUYEAST-2DPAGEiP17751
REPRODUCTION-2DPAGEiIPI00467833
P17751
SWISS-2DPAGEiP17751
UCD-2DPAGEiP17751

PTM databases

iPTMnetiP17751
PhosphoSitePlusiP17751
SwissPalmiP17751

Expressioni

Gene expression databases

BgeeiENSMUSG00000023456
CleanExiMM_TPI1
ExpressionAtlasiP17751 baseline and differential
GenevisibleiP17751 MM

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204290, 12 interactors
IntActiP17751, 15 interactors
MINTiP17751
STRINGi10090.ENSMUSP00000130858

Structurei

3D structure databases

ProteinModelPortaliP17751
SMRiP17751
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.Curated

Phylogenomic databases

eggNOGiKOG1643 Eukaryota
COG0149 LUCA
GeneTreeiENSGT00390000013354
HOVERGENiHBG002599
InParanoidiP17751
KOiK01803
OMAiLCVGEGL
OrthoDBiEOG091G0LEZ
TreeFamiTF300829

Family and domain databases

CDDicd00311 TIM, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00147_B TIM_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR035990 TIM_sf
IPR022896 TrioseP_Isoase_bac/euk
IPR000652 Triosephosphate_isomerase
IPR020861 Triosephosphate_isomerase_AS
PANTHERiPTHR21139 PTHR21139, 1 hit
PfamiView protein in Pfam
PF00121 TIM, 1 hit
SUPFAMiSSF51351 SSF51351, 1 hit
TIGRFAMsiTIGR00419 tim, 1 hit
PROSITEiView protein in PROSITE
PS00171 TIM_1, 1 hit
PS51440 TIM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P17751-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGKAEQQGA GLTMAEGGEK EEFCFTAIYI SGQWREPCVC TDLQRLEPGT
60 70 80 90 100
MAPTRKFFVG GNWKMNGRKK CLGELICTLN AANVPAGTEV VCAPPTAYID
110 120 130 140 150
FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDLGAT WVVLGHSERR
160 170 180 190 200
HVFGESDELI GQKVSHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV
210 220 230 240 250
IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVNDG
260 270 280 290
VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
Length:299
Mass (Da):32,192
Last modified:October 19, 2011 - v4
Checksum:iD465FD57108A3BD4
GO

Sequence cautioni

The sequence AAC36016 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH46761 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB27194 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE26832 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE29073 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE29810 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE35334 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE39523 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE40350 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE40594 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA37420 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124A → P in CAA37420 (PubMed:2377473).Curated1
Sequence conflicti157D → G in BAE39523 (PubMed:16141072).Curated1
Sequence conflicti244K → R in BAB27194 (PubMed:16141072).Curated1
Sequence conflicti245S → P in BAE26832 (PubMed:16141072).Curated1
Sequence conflicti266A → G in BAB27194 (PubMed:16141072).Curated1
Sequence conflicti272A → Q in BAB27194 (PubMed:16141072).Curated1
Sequence conflicti273 – 275SQP → TPA in CAA37420 (PubMed:2377473).Curated3
Sequence conflicti277V → M in BAB25634 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002397 Genomic DNA Translation: AAC36016.1 Different initiation.
AC142254 Genomic DNA No translation available.
AK008373 mRNA Translation: BAB25634.1
AK010808 mRNA Translation: BAB27194.1 Different initiation.
AK146013 mRNA Translation: BAE26832.1 Different initiation.
AK149768 mRNA Translation: BAE29073.1 Different initiation.
AK150735 mRNA Translation: BAE29810.1 Different initiation.
AK159741 mRNA Translation: BAE35334.1 Different initiation.
AK167437 mRNA Translation: BAE39523.1 Different initiation.
AK168446 mRNA Translation: BAE40350.1 Different initiation.
AK168756 mRNA Translation: BAE40594.1 Different initiation.
X53333 mRNA Translation: CAA37420.1 Different initiation.
BC046761 mRNA Translation: AAH46761.1 Different initiation.
L31777 Genomic DNA Translation: AAB48543.1
CCDSiCCDS20530.2
PIRiS10490 ISMST
RefSeqiNP_033441.2, NM_009415.2
UniGeneiMm.4222
Mm.439915

Genome annotation databases

EnsembliENSMUST00000172132; ENSMUSP00000130858; ENSMUSG00000023456
GeneIDi21991
KEGGimmu:21991
UCSCiuc012esp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTPIS_MOUSE
AccessioniPrimary (citable) accession number: P17751
Secondary accession number(s): Q3TJH2
, Q3UC04, Q3UKG9, Q64513, Q9CVF9, Q9CWE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 19, 2011
Last modified: May 23, 2018
This is version 168 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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