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P17744

- MTHC_HAEIF

UniProt

P17744 - MTHC_HAEIF

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Protein
Modification methylase HincII
Gene
hincIIM
Organism
Haemophilus influenzae
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GTYRAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HincII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3425. M.HincII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HincII (EC:2.1.1.72)
Short name:
M.HincII
Alternative name(s):
Adenine-specific methyltransferase HincII
Gene namesi
Name:hincIIM
OrganismiHaemophilus influenzae
Taxonomic identifieri727 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Modification methylase HincII
PRO_0000087973Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP17744.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF07669. Eco57I. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17744-1 [UniParc]FASTAAdd to Basket

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MDESKKISLG QFFTPTHIVK YMIGLMTKNK NASILEPSSG NGVFLDSLIQ    50
LGYTNLTSYE IDGDIISHPF VINSSFITSY DKPQYDSIIG NPPYVRWKNL 100
SELQKKELKD NSIWKMYCNS LCDYFYIFII KSILQLKVGG ELIFICPDYF 150
FSTKNAEGLR KFLINNGSFE KIILFNESKV FHGVSSSVVI FKYIKGKNID 200
NINIINIDSK SPIKSEDIES LGESYYIPRF SSSDVWVTSP NHIKVALDKF 250
ESYCKTIKKV QQKSLFDDLS FLDKAFQMND INYSELELLN SICVAKAKHL 300
DAFCFSGYTR YKLILDDNNE DKLITYFPNF FYEFNNYKDY LLKRYSYNKY 350
LPYWEVAFLR NFSLFSKNEK KIFVPCKERI SKKSNFRFSL VDEFIYPTQD 400
VTALYKKENV KESIEYITAY LNSKAVFLWM KYKGVVKGNV VEFSEKPLTN 450
IPFRRIDWQL KSEKKIHDDI TNLVRKYLSN KEFSILHEIN LNLEKLGIKV 500
EI 502
Length:502
Mass (Da):58,776
Last modified:August 1, 1990 - v1
Checksum:i2536FA72B2F1720A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52124 Genomic DNA. Translation: CAA36369.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52124 Genomic DNA. Translation: CAA36369.1 .

3D structure databases

ProteinModelPortali P17744.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3425. M.HincII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF07669. Eco57I. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, nucleotide sequence, and expression of the HincII restriction-modification system."
    Ito H., Sadaoka A., Kotani H., Hiraoka N., Nakamura T.
    Nucleic Acids Res. 18:3903-3911(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RC.

Entry informationi

Entry nameiMTHC_HAEIF
AccessioniPrimary (citable) accession number: P17744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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