P17744 (MTHC_HAEIF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase HincII
Short name=M.HincII
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase HincII

Gene names

Name:

hincIIM

Organism

Haemophilus influenzae

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GTYRAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HincII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Modification methylase HincII		PRO_0000087973

Sequences

	Sequence		Length	Mass (Da)
	P17744 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 2536FA72B2F1720A	FASTA	502	58,776
	10 20 30 40 50 60 MDESKKISLG QFFTPTHIVK YMIGLMTKNK NASILEPSSG NGVFLDSLIQ LGYTNLTSYE 70 80 90 100 110 120 IDGDIISHPF VINSSFITSY DKPQYDSIIG NPPYVRWKNL SELQKKELKD NSIWKMYCNS 130 140 150 160 170 180 LCDYFYIFII KSILQLKVGG ELIFICPDYF FSTKNAEGLR KFLINNGSFE KIILFNESKV 190 200 210 220 230 240 FHGVSSSVVI FKYIKGKNID NINIINIDSK SPIKSEDIES LGESYYIPRF SSSDVWVTSP 250 260 270 280 290 300 NHIKVALDKF ESYCKTIKKV QQKSLFDDLS FLDKAFQMND INYSELELLN SICVAKAKHL 310 320 330 340 350 360 DAFCFSGYTR YKLILDDNNE DKLITYFPNF FYEFNNYKDY LLKRYSYNKY LPYWEVAFLR 370 380 390 400 410 420 NFSLFSKNEK KIFVPCKERI SKKSNFRFSL VDEFIYPTQD VTALYKKENV KESIEYITAY 430 440 450 460 470 480 LNSKAVFLWM KYKGVVKGNV VEFSEKPLTN IPFRRIDWQL KSEKKIHDDI TNLVRKYLSN 490 500 KEFSILHEIN LNLEKLGIKV EI

References

[1]	"Cloning, nucleotide sequence, and expression of the HincII restriction-modification system." Ito H., Sadaoka A., Kotani H., Hiraoka N., Nakamura T. Nucleic Acids Res. 18:3903-3911(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52124 Genomic DNA. Translation: CAA36369.1.
3D structure databases
ProteinModelPortal	P17744.
ModBase	Search...
Protein family/group databases
REBASE	3425. M.HincII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR003356. DNA_methylase_A-5. IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. IPR011639. RM_methylase_Eco57I. [Graphical view]
Pfam	PF07669. Eco57I. 1 hit. PF02384. N6_Mtase. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTHC_HAEIF

Accession

Primary (citable) accession number: P17744

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program