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P17744 (MTHC_HAEIF) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HincII

Short name=M.HincII
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase HincII
Gene names
Name:hincIIM
OrganismHaemophilus influenzae
Taxonomic identifier727 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GTYRAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HincII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502Modification methylase HincII
PRO_0000087973

Sequences

Sequence LengthMass (Da)Tools
P17744 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 2536FA72B2F1720A

FASTA50258,776
        10         20         30         40         50         60 
MDESKKISLG QFFTPTHIVK YMIGLMTKNK NASILEPSSG NGVFLDSLIQ LGYTNLTSYE 

        70         80         90        100        110        120 
IDGDIISHPF VINSSFITSY DKPQYDSIIG NPPYVRWKNL SELQKKELKD NSIWKMYCNS 

       130        140        150        160        170        180 
LCDYFYIFII KSILQLKVGG ELIFICPDYF FSTKNAEGLR KFLINNGSFE KIILFNESKV 

       190        200        210        220        230        240 
FHGVSSSVVI FKYIKGKNID NINIINIDSK SPIKSEDIES LGESYYIPRF SSSDVWVTSP 

       250        260        270        280        290        300 
NHIKVALDKF ESYCKTIKKV QQKSLFDDLS FLDKAFQMND INYSELELLN SICVAKAKHL 

       310        320        330        340        350        360 
DAFCFSGYTR YKLILDDNNE DKLITYFPNF FYEFNNYKDY LLKRYSYNKY LPYWEVAFLR 

       370        380        390        400        410        420 
NFSLFSKNEK KIFVPCKERI SKKSNFRFSL VDEFIYPTQD VTALYKKENV KESIEYITAY 

       430        440        450        460        470        480 
LNSKAVFLWM KYKGVVKGNV VEFSEKPLTN IPFRRIDWQL KSEKKIHDDI TNLVRKYLSN 

       490        500 
KEFSILHEIN LNLEKLGIKV EI 

« Hide

References

[1]"Cloning, nucleotide sequence, and expression of the HincII restriction-modification system."
Ito H., Sadaoka A., Kotani H., Hiraoka N., Nakamura T.
Nucleic Acids Res. 18:3903-3911(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: RC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52124 Genomic DNA. Translation: CAA36369.1.

3D structure databases

ProteinModelPortalP17744.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3425. M.HincII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF07669. Eco57I. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTHC_HAEIF
AccessionPrimary (citable) accession number: P17744
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries