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P17744

- MTHC_HAEIF

UniProt

P17744 - MTHC_HAEIF

Protein

Modification methylase HincII

Gene

hincIIM

Organism
Haemophilus influenzae
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GTYRAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HincII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3425. M.HincII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HincII (EC:2.1.1.72)
    Short name:
    M.HincII
    Alternative name(s):
    Adenine-specific methyltransferase HincII
    Gene namesi
    Name:hincIIM
    OrganismiHaemophilus influenzae
    Taxonomic identifieri727 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502Modification methylase HincIIPRO_0000087973Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP17744.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF07669. Eco57I. 1 hit.
    PF02384. N6_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17744-1 [UniParc]FASTAAdd to Basket

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    MDESKKISLG QFFTPTHIVK YMIGLMTKNK NASILEPSSG NGVFLDSLIQ    50
    LGYTNLTSYE IDGDIISHPF VINSSFITSY DKPQYDSIIG NPPYVRWKNL 100
    SELQKKELKD NSIWKMYCNS LCDYFYIFII KSILQLKVGG ELIFICPDYF 150
    FSTKNAEGLR KFLINNGSFE KIILFNESKV FHGVSSSVVI FKYIKGKNID 200
    NINIINIDSK SPIKSEDIES LGESYYIPRF SSSDVWVTSP NHIKVALDKF 250
    ESYCKTIKKV QQKSLFDDLS FLDKAFQMND INYSELELLN SICVAKAKHL 300
    DAFCFSGYTR YKLILDDNNE DKLITYFPNF FYEFNNYKDY LLKRYSYNKY 350
    LPYWEVAFLR NFSLFSKNEK KIFVPCKERI SKKSNFRFSL VDEFIYPTQD 400
    VTALYKKENV KESIEYITAY LNSKAVFLWM KYKGVVKGNV VEFSEKPLTN 450
    IPFRRIDWQL KSEKKIHDDI TNLVRKYLSN KEFSILHEIN LNLEKLGIKV 500
    EI 502
    Length:502
    Mass (Da):58,776
    Last modified:August 1, 1990 - v1
    Checksum:i2536FA72B2F1720A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52124 Genomic DNA. Translation: CAA36369.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52124 Genomic DNA. Translation: CAA36369.1 .

    3D structure databases

    ProteinModelPortali P17744.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3425. M.HincII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF07669. Eco57I. 1 hit.
    PF02384. N6_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, nucleotide sequence, and expression of the HincII restriction-modification system."
      Ito H., Sadaoka A., Kotani H., Hiraoka N., Nakamura T.
      Nucleic Acids Res. 18:3903-3911(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RC.

    Entry informationi

    Entry nameiMTHC_HAEIF
    AccessioniPrimary (citable) accession number: P17744
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3