Reviewed, UniProtKB/Swiss-Prot P17743 (T2C2_HAEIN)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme HincII
      Short name=R.HincII
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme HincII
    Endonuclease HincII

Gene names

Name:

hincIIR

Organism

Haemophilus influenzae

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GTYRAC and cleaves after Y-3.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 258

257

Type-2 restriction enzyme HincII

PRO_0000077319

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

258

Helix Strand Turn

Details...

Helix

5 – 7

3

Helix

8 – 15

8

Beta strand

19 – 21

3

Helix

35 – 48

14

Turn

50 – 52

3

Beta strand

53 – 55

3

Helix

56 – 65

10

Helix

73 – 76

4

Helix

77 – 80

4

Helix

83 – 89

7

Helix

93 – 98

6

Beta strand

114 – 119

6

Beta strand

122 – 132

11

Beta strand

141 – 144

4

Helix

145 – 158

14

Beta strand

162 – 176

15

Beta strand

179 – 189

11

Helix

190 – 192

3

Helix

195 – 197

3

Beta strand

199 – 202

4

Beta strand

207 – 209

3

Helix

212 – 214

3

Helix

223 – 249

27

Turn

250 – 253

4

Helix

254 – 256

3

Sequences

	Sequence		Length	Mass (Da)
	P17743-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 9FE82017F7C15A47	FASTA	258	29,871
	10 20 30 40 50 60 MSFIKPIYQD INSILIGQKV KRPKSGTLSG HAAGEPFEKL VYKFLKENLS DLTFKQYEYL 70 80 90 100 110 120 NDLFMKNPAI IGHEARYKLF NSPTLLFLLS RGKAATENWS IENLFEEKQN DTADILLVKD 130 140 150 160 170 180 QFYELLDVKR RNISKSAQAP NIISAYKLAQ TCAKMIDNKE FDLFDINYLE VDSELNGEDL 190 200 210 220 230 240 VCVSTSFAEL FKSEPSELYI NWAAAMQIQF HVRDLDQGFN GTREEWAKSY LKHFVTQAEQ 250 RAISMIDKFV KPFKKYIL

References

[1]	"Cloning, nucleotide sequence, and expression of the HincII restriction-modification system." Ito H., Sadaoka A., Kotani H., Hiraoka N., Nakamura T. Nucleic Acids Res. 18:3903-3911(1990) [PubMed: 2374714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RC.
[2]	"Sequence selectivity and degeneracy of a restriction endonuclease mediated by DNA intercalation." Horton N.C., Dorner L.F., Perona J.J. Nat. Struct. Biol. 9:42-47(2002) [PubMed: 11742344] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X52124 Genomic DNA. Translation: CAA36370.1.

PIR

S10323.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KC6	X-ray	2.60	A/B/C/D	2-258	[»]
1XHU	X-ray	2.95	A/B/C/D	2-257	[»]
1XHV	X-ray	2.50	A/B/C/D	2-257	[»]

ModBase

Search...

Protein family/group databases

REBASE

1148. HincII.

Enzyme and pathway databases

BRENDA

3.1.21.4. 109.

Family and domain databases

InterPro

IPR011337. Restrict_endonuc_II/DNA_repair.
IPR015307. Restrict_endonuc_II_HincII.
[Graphical view]

Gene3D

G3DSA:3.40.600.10. Restrict_endonuc_II/DNA_repair. 1 hit.

Pfam

PF09226. Endonuc-HincII. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

T2C2_HAEIN

Accession

Primary (citable) accession number: P17743

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)