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P17743

- T2C2_HAEIF

UniProt

P17743 - T2C2_HAEIF

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Protein

Type-2 restriction enzyme HincII

Gene
hincIIR
Organism
Haemophilus influenzae
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GTYRAC and cleaves after Y-3.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1148. HincII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme HincII (EC:3.1.21.4)
Short name:
R.HincII
Alternative name(s):
Endonuclease HincII
Type II restriction enzyme HincII
Gene namesi
Name:hincIIR
OrganismiHaemophilus influenzae
Taxonomic identifieri727 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 258257Type-2 restriction enzyme HincIIPRO_0000077319Add
BLAST

Interactioni

Protein-protein interaction databases

DIPiDIP-48338N.

Structurei

Secondary structure

1
258
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 73
Helixi8 – 158
Beta strandi19 – 213
Beta strandi24 – 263
Helixi30 – 323
Turni33 – 364
Helixi37 – 4812
Turni50 – 523
Beta strandi53 – 553
Helixi56 – 6510
Helixi73 – 786
Helixi83 – 897
Helixi93 – 986
Beta strandi101 – 1033
Beta strandi114 – 1196
Beta strandi122 – 13211
Beta strandi141 – 1444
Helixi145 – 15814
Beta strandi162 – 17615
Beta strandi179 – 18911
Helixi190 – 1923
Helixi195 – 1973
Beta strandi200 – 2023
Turni203 – 2064
Beta strandi207 – 2093
Helixi212 – 2143
Helixi223 – 24927
Helixi251 – 2566

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KC6X-ray2.60A/B/C/D2-258[»]
1TW8X-ray2.80A/B/C/D2-258[»]
1TX3X-ray2.50A/B/C/D2-258[»]
1XHUX-ray2.95A/B/C/D2-258[»]
1XHVX-ray2.50A/B/C/D2-258[»]
2AUDX-ray2.10A2-258[»]
2GIEX-ray2.60A/B/C/D2-258[»]
2GIGX-ray1.83A/B2-258[»]
2GIHX-ray2.50A/B2-258[»]
2GIIX-ray2.30A/B2-258[»]
2GIJX-ray1.93A/B2-258[»]
3E3YX-ray2.13A/B2-258[»]
3E40X-ray2.10A/B2-258[»]
3E41X-ray2.73A/B2-258[»]
3E42X-ray2.68A/B2-258[»]
3E43X-ray2.73A/B2-258[»]
3E44X-ray2.52A/B2-258[»]
3E45X-ray2.78A/B2-258[»]
3EBCX-ray2.55A/B1-258[»]
ProteinModelPortaliP17743.
SMRiP17743. Positions 2-258.

Miscellaneous databases

EvolutionaryTraceiP17743.

Family & Domainsi

Family and domain databases

Gene3Di3.40.600.10. 1 hit.
InterProiIPR011337. DNA_rep_MutH/RE_typeII.
IPR011335. Restrct_endonuc-II-like.
IPR015307. Restrct_endonuc_II_HincII.
[Graphical view]
PfamiPF09226. Endonuc-HincII. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17743-1 [UniParc]FASTAAdd to Basket

« Hide

MSFIKPIYQD INSILIGQKV KRPKSGTLSG HAAGEPFEKL VYKFLKENLS    50
DLTFKQYEYL NDLFMKNPAI IGHEARYKLF NSPTLLFLLS RGKAATENWS 100
IENLFEEKQN DTADILLVKD QFYELLDVKR RNISKSAQAP NIISAYKLAQ 150
TCAKMIDNKE FDLFDINYLE VDSELNGEDL VCVSTSFAEL FKSEPSELYI 200
NWAAAMQIQF HVRDLDQGFN GTREEWAKSY LKHFVTQAEQ RAISMIDKFV 250
KPFKKYIL 258
Length:258
Mass (Da):29,871
Last modified:January 23, 2007 - v3
Checksum:i9FE82017F7C15A47
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52124 Genomic DNA. Translation: CAA36370.1.
PIRiS10323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52124 Genomic DNA. Translation: CAA36370.1 .
PIRi S10323.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KC6 X-ray 2.60 A/B/C/D 2-258 [» ]
1TW8 X-ray 2.80 A/B/C/D 2-258 [» ]
1TX3 X-ray 2.50 A/B/C/D 2-258 [» ]
1XHU X-ray 2.95 A/B/C/D 2-258 [» ]
1XHV X-ray 2.50 A/B/C/D 2-258 [» ]
2AUD X-ray 2.10 A 2-258 [» ]
2GIE X-ray 2.60 A/B/C/D 2-258 [» ]
2GIG X-ray 1.83 A/B 2-258 [» ]
2GIH X-ray 2.50 A/B 2-258 [» ]
2GII X-ray 2.30 A/B 2-258 [» ]
2GIJ X-ray 1.93 A/B 2-258 [» ]
3E3Y X-ray 2.13 A/B 2-258 [» ]
3E40 X-ray 2.10 A/B 2-258 [» ]
3E41 X-ray 2.73 A/B 2-258 [» ]
3E42 X-ray 2.68 A/B 2-258 [» ]
3E43 X-ray 2.73 A/B 2-258 [» ]
3E44 X-ray 2.52 A/B 2-258 [» ]
3E45 X-ray 2.78 A/B 2-258 [» ]
3EBC X-ray 2.55 A/B 1-258 [» ]
ProteinModelPortali P17743.
SMRi P17743. Positions 2-258.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48338N.

Protein family/group databases

REBASEi 1148. HincII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P17743.

Family and domain databases

Gene3Di 3.40.600.10. 1 hit.
InterProi IPR011337. DNA_rep_MutH/RE_typeII.
IPR011335. Restrct_endonuc-II-like.
IPR015307. Restrct_endonuc_II_HincII.
[Graphical view ]
Pfami PF09226. Endonuc-HincII. 1 hit.
[Graphical view ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning, nucleotide sequence, and expression of the HincII restriction-modification system."
    Ito H., Sadaoka A., Kotani H., Hiraoka N., Nakamura T.
    Nucleic Acids Res. 18:3903-3911(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RC.
  2. "Sequence selectivity and degeneracy of a restriction endonuclease mediated by DNA intercalation."
    Horton N.C., Dorner L.F., Perona J.J.
    Nat. Struct. Biol. 9:42-47(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 2-258.
  3. "Ca2+ binding in the active site of HincII: implications for the catalytic mechanism."
    Etzkorn C., Horton N.C.
    Biochemistry 43:13256-13270(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 2-257.
  4. "Mechanistic insights from the structures of HincII bound to cognate DNA cleaved from addition of Mg2+ and Mn2+."
    Etzkorn C., Horton N.C.
    J. Mol. Biol. 343:833-849(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.95 ANGSTROMS) OF 2-257.
  5. "DNA-induced conformational changes in type II restriction endonucleases: the structure of unliganded HincII."
    Little E.J., Horton N.C.
    J. Mol. Biol. 351:76-88(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 2-257.
  6. "Alteration of sequence specificity of the type II restriction endonuclease HincII through an indirect readout mechanism."
    Joshi H.K., Etzkorn C., Chatwell L., Bitinaite J., Horton N.C.
    J. Biol. Chem. 281:23852-23869(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 2-258.
  7. "DNA distortion and specificity in a sequence-specific endonuclease."
    Babic A.C., Little E.J., Manohar V.M., Bitinaite J., Horton N.C.
    J. Mol. Biol. 383:186-204(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.13 ANGSTROMS) OF 2-258.
  8. "Early interrogation and recognition of DNA sequence by indirect readout."
    Little E.J., Babic A.C., Horton N.C.
    Structure 16:1828-1837(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS).

Entry informationi

Entry nameiT2C2_HAEIF
AccessioniPrimary (citable) accession number: P17743
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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