Reviewed, UniProtKB/Swiss-Prot P17741 (HMGB2_PIG)

Names and origin

Protein names

Recommended name:
    High mobility group protein B2
Alternative name(s):
    High mobility group protein 2
      Short name=HMG-2

Gene names

Name:	HMGB2
Synonyms:	HMG2

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds preferentially single-stranded DNA and unwinds double stranded DNA.
Subunit structure	Component of the SET complex, which also contains SET, ANP32A, APEX1 and NME1. Directly interacts with SET By similarity.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains.

Ontologies

Keywords
Cellular component	Chromosomal protein Nucleus
Domain	Repeat
Ligand	DNA-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	base-excision repair, DNA ligation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	condensed chromosome Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	DNA bending activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 210

209

High mobility group protein B2

PRO_0000048536

Regions

DNA binding

9 – 79

71

HMG box 1

DNA binding

95 – 163

69

HMG box 2

Compositional bias

186 – 210

25

Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue

30

1

N6-acetyllysine By similarity

Modified residue

35

1

Phosphoserine By similarity

Modified residue

59

1

N6-acetyllysine By similarity

Modified residue

155

1

Phosphotyrosine By similarity

Modified residue

162

1

Phosphotyrosine By similarity

Modified residue

172

1

N6-acetyllysine By similarity

Modified residue

173

1

N6-acetyllysine By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

210

Helix Strand Turn

Details...

Beta strand

6 – 8

3

Helix

15 – 25

11

Helix

28 – 30

3

Helix

38 – 51

14

Helix

54 – 70

17

Turn

71 – 74

4

Beta strand

92 – 94

3

Helix

102 – 116

15

Helix

122 – 135

14

Helix

147 – 149

3

Helix

152 – 163

12

Sequences

	Sequence		Length	Mass (Da)
	P17741-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 28FF308D9B341D83	FASTA	210	24,133
	10 20 30 40 50 60 MGKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK TMSAKEKSKF 70 80 90 100 110 120 EDMAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS AFFLFCSEHR PKIKSEHPGL 130 140 150 160 170 180 SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK LKEKYEKDIA AYRAKGKGEA GKKGPGRPTG 190 200 210 SKKKNEPEDE EEEEEEEEDE DEEEEDEDEE

References

[1]	"Primary structure of non-histone chromosomal protein HMG2 revealed by the nucleotide sequence." Shirakawa H., Tsuda K., Yoshida M. Biochemistry 29:4419-4423(1990) [PubMed: 2350545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Thymus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J02895 mRNA. Translation: AAA31051.1.

PIR

A34719.

RefSeq

NP_999228.1.

UniGene

Ssc.226

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J3C	NMR	-	A	88-164	[»]
1J3D	NMR	-	A	89-164	[»]
1J3X	NMR	-	A	1-77	[»]

SMR

P17741. Positions 5-166.

ModBase

Search...

Genome annotation databases

Ensembl

ENSSSCT00000010639; ENSSSCP00000010363; ENSSSCG00000009704; Sus scrofa. [Genome view]

GeneID

397130.

KEGG

ssc:397130.

Organism-specific databases

CTD

397130.

Phylogenomic databases

HOVERGEN

P17741.

Family and domain databases

InterPro

IPR017967. HMG_boxA_CS.
IPR000910. HMG_HMG1/HMG2.
IPR000135. HMG_HMG1/HMG2_subgr.
IPR009071. HMG_superfamily.
[Graphical view]

Gene3D

G3DSA:1.10.30.10. HMG-box. 2 hits.

Pfam

PF00505. HMG_box. 2 hits.
[Graphical view]

PRINTS

PR00886. HIGHMOBLTY12.

SMART

SM00398. HMG. 2 hits.
[Graphical view]

PROSITE

PS00353. HMG_BOX_1. 1 hit.
PS50118. HMG_BOX_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HMGB2_PIG

Accession

Primary (citable) accession number: P17741

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)