P17726 (TAP_ORNMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Tick anticoagulant peptide Short name=TAP
Organism	Ornithodoros moubata (Soft tick) (Argasid tick)
Taxonomic identifier	6938 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Acari › Parasitiformes › Ixodida › Ixodoidea › Argasidae › Ornithodorinae › Ornithodoros

Protein attributes

Sequence length	60 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TAP is a slow, tight-binding inhibitor of blood coagulation, specific for factor Xa.
Miscellaneous	The inhibition of factor Xa seems to be reversible and stoichiometric.
Sequence similarities	Contains 1 BPTI/Kunitz inhibitor domain.

Ontologies

Keywords
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 60

60

Tick anticoagulant peptide

PRO_0000155457

Regions

Domain

5 – 59

55

BPTI/Kunitz inhibitor

Amino acid modifications

Disulfide bond

5 ↔ 59

Ref.2

Disulfide bond

15 ↔ 39

Ref.2

Disulfide bond

33 ↔ 55

Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

60

Helix Strand Turn

Details...

Helix

3 – 5

3

Turn

11 – 14

4

Helix

17 – 19

3

Beta strand

22 – 27

6

Beta strand

29 – 38

10

Turn

40 – 42

3

Helix

52 – 59

8

Sequences

	Sequence		Length	Mass (Da)
	P17726 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 700D3D1245E83099	FASTA	60	6,985
	10 20 30 40 50 60 YNRLCIKPRD WIDECDSNEG GERAYFRNGK GGCDSFWICP EDHTGADYYS SYRDCFNACI

References

[1]	"Tick anticoagulant peptide (TAP) is a novel inhibitor of blood coagulation factor Xa." Waxman L., Smith D.E., Arcuri K.E., Vlasuk G.P. Science 248:593-596(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Determination of disulfide bond pairs and stability in recombinant tick anticoagulant peptide." Sardana M., Sardana V., Rodkey J., Wood T., Ng A., Vlasuk G.P., Waxman L. J. Biol. Chem. 266:13560-13563(1991) [PubMed] [Europe PMC] [Abstract] Cited for: DISULFIDE BONDS.
[3]	"NMR solution structure of the recombinant tick anticoagulant protein (rTAP), a factor Xa inhibitor from the tick Ornithodoros moubata." Antuch W., Guntert P., Billeter M., Hawthorne T., Grossenbacher H., Wuethrich K. FEBS Lett. 352:251-257(1994) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[4]	"NMR structure determination of tick anticoagulant peptide (TAP)." Lim-Wilby M.S.L., Hallenga K., de Maeyer M., Lasters I., Vlasuk G.P., Brunck T.K. Protein Sci. 4:178-186(1995) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

A41212.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D0D	X-ray	1.62	A	1-60	[»]
1KIG	X-ray	3.00	I	1-60	[»]
1TAP	NMR	-	A	1-60	[»]
1TCP	NMR	-	A	1-60	[»]

ProteinModelPortal

P17726.

SMR

P17726. Positions 1-60.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-124564.

Protein family/group databases

MEROPS

I52.001.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

4.10.410.10. 1 hit.

InterPro

IPR002223. Prot_inh_Kunz-m.
[Graphical view]

Pfam

PF00014. Kunitz_BPTI. 1 hit.
[Graphical view]

SUPFAM

SSF57362. Prot_inh_Kunz-m. 1 hit.

PROSITE

PS00280. BPTI_KUNITZ_1. False negative.
PS50279. BPTI_KUNITZ_2. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P17726.

Entry information

Entry name

TAP_ORNMO

Accession

Primary (citable) accession number: P17726

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)