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Protein

Glucokinase-1

Gene

GLK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Two isoenzymes, hexokinase-1 and hexokinase-2, can phosphorylate keto- and aldohexoses in yeast, whereas a third isoenzyme, GLK, is specific for aldohexoses. All glucose phosphorylating enzymes are involved in glucose uptake.

Catalytic activityi

ATP + D-glucose = ADP + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101ATPSequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi487 – 4926ATPSequence Analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glucokinase activity Source: SGD

GO - Biological processi

  • glucose 6-phosphate metabolic process Source: GOC
  • glucose import Source: SGD
  • glucose metabolic process Source: SGD
  • glycolytic process Source: SGD
  • mannose metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-5982.
YEAST:YCL040W-MONOMER.
BRENDAi2.7.1.2. 984.
ReactomeiREACT_275254. Glycolysis.
REACT_298577. Regulation of gene expression in beta cells.
REACT_302131. Glucose transport.
REACT_318658. Regulation of Glucokinase by Glucokinase Regulatory Protein.
SABIO-RKP17709.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucokinase-1 (EC:2.7.1.2)
Alternative name(s):
Glucose kinase 1
Short name:
GLK-1
Gene namesi
Name:GLK1
Synonyms:HOR3
Ordered Locus Names:YCL040W
ORF Names:YCL312, YCL40W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome III

Organism-specific databases

CYGDiYCL040w.
EuPathDBiFungiDB:YCL040W.
SGDiS000000545. GLK1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 500499Glucokinase-1PRO_0000197603Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications
Modified residuei2 – 21Phosphoserine1 Publication
Modified residuei470 – 4701Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP17709.
PaxDbiP17709.
PeptideAtlasiP17709.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi30943. 30 interactions.
DIPiDIP-525N.
IntActiP17709. 5 interactions.
MINTiMINT-401461.

Structurei

3D structure databases

ProteinModelPortaliP17709.
SMRiP17709. Positions 25-497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 498487HexokinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni74 – 216143Hexokinase small subdomainPROSITE-ProRule annotationAdd
BLAST
Regioni158 – 18427Glucose-bindingSequence AnalysisAdd
BLAST
Regioni217 – 487271Hexokinase large subdomainPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 1 hexokinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162670.
InParanoidiP17709.
KOiK00844.
OrthoDBiEOG79SF68.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17709-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFDDLHKAT ERAVIQAVDQ ICDDFEVTPE KLDELTAYFI EQMEKGLAPP
60 70 80 90 100
KEGHTLASDK GLPMIPAFVT GSPNGTERGV LLAADLGGTN FRICSVNLHG
110 120 130 140 150
DHTFSMEQMK SKIPDDLLDD ENVTSDDLFG FLARRTLAFM KKYHPDELAK
160 170 180 190 200
GKDAKPMKLG FTFSYPVDQT SLNSGTLIRW TKGFRIADTV GKDVVQLYQE
210 220 230 240 250
QLSAQGMPMI KVVALTNDTV GTYLSHCYTS DNTDSMTSGE ISEPVIGCIF
260 270 280 290 300
GTGTNGCYME EINKITKLPQ ELRDKLIKEG KTHMIINVEW GSFDNELKHL
310 320 330 340 350
PTTKYDVVID QKLSTNPGFH LFEKRVSGMF LGEVLRNILV DLHSQGLLLQ
360 370 380 390 400
QYRSKEQLPR HLTTPFQLSS EVLSHIEIDD STGLRETELS LLQSLRLPTT
410 420 430 440 450
PTERVQIQKL VRAISRRSAY LAAVPLAAIL IKTNALNKRY HGEVEIGCDG
460 470 480 490 500
SVVEYYPGFR SMLRHALALS PLGAEGERKV HLKIAKDGSG VGAALCALVA
Length:500
Mass (Da):55,377
Last modified:August 1, 1990 - v1
Checksum:iA7A417041D7A961F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24077 Genomic DNA. Translation: AAA53536.1.
X59720 Genomic DNA. Translation: CAA42376.1.
BK006937 Genomic DNA. Translation: DAA07444.1.
PIRiJT0482.
RefSeqiNP_009890.1. NM_001178685.1.

Genome annotation databases

EnsemblFungiiYCL040W; YCL040W; YCL040W.
GeneIDi850317.
KEGGisce:YCL040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24077 Genomic DNA. Translation: AAA53536.1.
X59720 Genomic DNA. Translation: CAA42376.1.
BK006937 Genomic DNA. Translation: DAA07444.1.
PIRiJT0482.
RefSeqiNP_009890.1. NM_001178685.1.

3D structure databases

ProteinModelPortaliP17709.
SMRiP17709. Positions 25-497.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30943. 30 interactions.
DIPiDIP-525N.
IntActiP17709. 5 interactions.
MINTiMINT-401461.

Proteomic databases

MaxQBiP17709.
PaxDbiP17709.
PeptideAtlasiP17709.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYCL040W; YCL040W; YCL040W.
GeneIDi850317.
KEGGisce:YCL040W.

Organism-specific databases

CYGDiYCL040w.
EuPathDBiFungiDB:YCL040W.
SGDiS000000545. GLK1.

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162670.
InParanoidiP17709.
KOiK00844.
OrthoDBiEOG79SF68.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-5982.
YEAST:YCL040W-MONOMER.
BRENDAi2.7.1.2. 984.
ReactomeiREACT_275254. Glycolysis.
REACT_298577. Regulation of gene expression in beta cells.
REACT_302131. Glucose transport.
REACT_318658. Regulation of Glucokinase by Glucokinase Regulatory Protein.
SABIO-RKP17709.

Miscellaneous databases

NextBioi965721.
PROiP17709.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 1 hit.
PS51748. HEXOKINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of yeast glucokinase, a strongly diverged specific aldo-hexose-phosphorylating isoenzyme."
    Albig W., Entian K.-D.
    Gene 73:141-152(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete sequence of a 9,543 bp segment on the left arm of chromosome III reveals five open reading frames including glucokinase and the protein disulfide isomerase."
    Scherens B., Messenguy F., Gigot D., Dubois E.
    Yeast 8:577-586(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-470, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHXKG_YEAST
AccessioniPrimary (citable) accession number: P17709
Secondary accession number(s): D6VQX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 22, 2015
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 21100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.