Reviewed, UniProtKB/Swiss-Prot P17709 (HXKG_YEAST)

Names and origin

Protein names

Recommended name:
    Glucokinase-1
    EC=2.7.1.2
Alternative name(s):
    Glucose kinase 1
      Short name=GLK-1

Gene names

Name:	GLK1
Synonyms:	HOR3
Ordered Locus Names:	YCL040W
ORF Names:	YCL40W, YCL312

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Two isoenzymes, hexokinase-1 and hexokinase-2, can phosphorylate keto- and aldohexoses in yeast, whereas a third isoenzyme, GLK, is specific for aldohexoses. All glucose phosphorylating enzymes are involved in glucose uptake.
Catalytic activity	ATP + D-glucose = ADP + D-glucose 6-phosphate.
Subunit structure	Monomer.
Miscellaneous	Present with 21100 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose import Inferred from genetic interaction. Source: SGD glycolysis Inferred from direct assay. Source: SGD mannose metabolic process Inferred from direct assay. Source: SGD
Cellular component	cytosol Inferred from direct assay. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glucokinase activity Inferred from direct assay. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-8744,EBI-8744
SRP1	Q02821	1	EBI-8744,EBI-1797

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Glucokinase-1		PRO_0000197603
Regions
	Nucleotide binding	487 – 492	6	ATP Potential
	Region	158 – 184	27	Glucose-binding Potential
Sites
	Binding site	110	1	ATP Potential
Amino acid modifications
	Modified residue	2	1	Phosphoserine Ref.5
	Modified residue	174	1	Phosphoserine Ref.5
	Modified residue	470	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P17709-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: A7A417041D7A961F	FASTA	500	55,377
	10 20 30 40 50 60 MSFDDLHKAT ERAVIQAVDQ ICDDFEVTPE KLDELTAYFI EQMEKGLAPP KEGHTLASDK 70 80 90 100 110 120 GLPMIPAFVT GSPNGTERGV LLAADLGGTN FRICSVNLHG DHTFSMEQMK SKIPDDLLDD 130 140 150 160 170 180 ENVTSDDLFG FLARRTLAFM KKYHPDELAK GKDAKPMKLG FTFSYPVDQT SLNSGTLIRW 190 200 210 220 230 240 TKGFRIADTV GKDVVQLYQE QLSAQGMPMI KVVALTNDTV GTYLSHCYTS DNTDSMTSGE 250 260 270 280 290 300 ISEPVIGCIF GTGTNGCYME EINKITKLPQ ELRDKLIKEG KTHMIINVEW GSFDNELKHL 310 320 330 340 350 360 PTTKYDVVID QKLSTNPGFH LFEKRVSGMF LGEVLRNILV DLHSQGLLLQ QYRSKEQLPR 370 380 390 400 410 420 HLTTPFQLSS EVLSHIEIDD STGLRETELS LLQSLRLPTT PTERVQIQKL VRAISRRSAY 430 440 450 460 470 480 LAAVPLAAIL IKTNALNKRY HGEVEIGCDG SVVEYYPGFR SMLRHALALS PLGAEGERKV 490 500 HLKIAKDGSG VGAALCALVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of yeast glucokinase, a strongly diverged specific aldo-hexose-phosphorylating isoenzyme." Albig W., Entian K.-D. Gene 73:141-152(1988) [PubMed: 3072253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete sequence of a 9,543 bp segment on the left arm of chromosome III reveals five open reading frames including glucokinase and the protein disulfide isomerase." Scherens B., Messenguy F., Gigot D., Dubois E. Yeast 8:577-586(1992) [PubMed: 1523890] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-174 AND SER-470, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M24077 Genomic DNA. Translation: AAA53536.1. X59720 Genomic DNA. Translation: CAA42376.1.
PIR	JT0482.
RefSeq	NP_009890.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-525N.
IntAct	P17709. 7 interactions.
STRING	P17709.
Proteomic databases
PeptideAtlas	P17709.
PRIDE	P17709.
Genome annotation databases
Ensembl	YCL040W; YCL040W; YCL040W; Saccharomyces cerevisiae. [Genome view]
GeneID	850317.
KEGG	sce:YCL040W.
NMPDR	fig|4932.3.peg.610.
Organism-specific databases
CYGD	YCL040w.
SGD	S000000545. GLK1.
Phylogenomic databases
eggNOG	fuNOG07704.
HOGENOM	HBG522186.
OMA	NGCYMEE.
OrthoDB	EOG95HTDS.
PhylomeDB	P17709.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-5982.
BRENDA	2.7.1.2. 250.
Gene expression databases
ArrayExpress	P17709.
Genevestigator	P17709.
GermOnline	YCL040W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR019807. Hexokinase_CS. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
PROSITE	PS00378. HEXOKINASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	965721.

Entry information

Entry name

HXKG_YEAST

Accession

Primary (citable) accession number: P17709

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	January 19, 2010
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)