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P17709

- HXKG_YEAST

UniProt

P17709 - HXKG_YEAST

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Protein

Glucokinase-1

Gene
GLK1, HOR3, YCL040W, YCL312, YCL40W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Two isoenzymes, hexokinase-1 and hexokinase-2, can phosphorylate keto- and aldohexoses in yeast, whereas a third isoenzyme, GLK, is specific for aldohexoses. All glucose phosphorylating enzymes are involved in glucose uptake.

Catalytic activityi

ATP + D-glucose = ADP + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101ATP Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi487 – 4926ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glucokinase activity Source: SGD

GO - Biological processi

  1. glucose 6-phosphate metabolic process Source: GOC
  2. glucose import Source: SGD
  3. glucose metabolic process Source: SGD
  4. glycolytic process Source: SGD
  5. mannose metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-5982.
YEAST:YCL040W-MONOMER.
SABIO-RKP17709.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucokinase-1 (EC:2.7.1.2)
Alternative name(s):
Glucose kinase 1
Short name:
GLK-1
Gene namesi
Name:GLK1
Synonyms:HOR3
Ordered Locus Names:YCL040W
ORF Names:YCL312, YCL40W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCL040w.
SGDiS000000545. GLK1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 500499Glucokinase-1PRO_0000197603Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications
Modified residuei2 – 21Phosphoserine1 Publication
Modified residuei470 – 4701Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP17709.
PaxDbiP17709.
PeptideAtlasiP17709.

Expressioni

Gene expression databases

GenevestigatoriP17709.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi30943. 29 interactions.
DIPiDIP-525N.
IntActiP17709. 5 interactions.
MINTiMINT-401461.
STRINGi4932.YCL040W.

Structurei

3D structure databases

ProteinModelPortaliP17709.
SMRiP17709. Positions 25-497.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 230217Hexokinase type-1Add
BLAST
Domaini244 – 500257Hexokinase type-2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni158 – 18427Glucose-binding Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the hexokinase family.

Phylogenomic databases

eggNOGiCOG5026.
GeneTreeiENSGT00390000017159.
HOGENOMiHOG000162670.
KOiK00844.
OMAiGEVEIGC.
OrthoDBiEOG79SF68.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
PROSITEiPS00378. HEXOKINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17709-1 [UniParc]FASTAAdd to Basket

« Hide

MSFDDLHKAT ERAVIQAVDQ ICDDFEVTPE KLDELTAYFI EQMEKGLAPP    50
KEGHTLASDK GLPMIPAFVT GSPNGTERGV LLAADLGGTN FRICSVNLHG 100
DHTFSMEQMK SKIPDDLLDD ENVTSDDLFG FLARRTLAFM KKYHPDELAK 150
GKDAKPMKLG FTFSYPVDQT SLNSGTLIRW TKGFRIADTV GKDVVQLYQE 200
QLSAQGMPMI KVVALTNDTV GTYLSHCYTS DNTDSMTSGE ISEPVIGCIF 250
GTGTNGCYME EINKITKLPQ ELRDKLIKEG KTHMIINVEW GSFDNELKHL 300
PTTKYDVVID QKLSTNPGFH LFEKRVSGMF LGEVLRNILV DLHSQGLLLQ 350
QYRSKEQLPR HLTTPFQLSS EVLSHIEIDD STGLRETELS LLQSLRLPTT 400
PTERVQIQKL VRAISRRSAY LAAVPLAAIL IKTNALNKRY HGEVEIGCDG 450
SVVEYYPGFR SMLRHALALS PLGAEGERKV HLKIAKDGSG VGAALCALVA 500
Length:500
Mass (Da):55,377
Last modified:August 1, 1990 - v1
Checksum:iA7A417041D7A961F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24077 Genomic DNA. Translation: AAA53536.1.
X59720 Genomic DNA. Translation: CAA42376.1.
BK006937 Genomic DNA. Translation: DAA07444.1.
PIRiJT0482.
RefSeqiNP_009890.1. NM_001178685.1.

Genome annotation databases

EnsemblFungiiYCL040W; YCL040W; YCL040W.
GeneIDi850317.
KEGGisce:YCL040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24077 Genomic DNA. Translation: AAA53536.1 .
X59720 Genomic DNA. Translation: CAA42376.1 .
BK006937 Genomic DNA. Translation: DAA07444.1 .
PIRi JT0482.
RefSeqi NP_009890.1. NM_001178685.1.

3D structure databases

ProteinModelPortali P17709.
SMRi P17709. Positions 25-497.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30943. 29 interactions.
DIPi DIP-525N.
IntActi P17709. 5 interactions.
MINTi MINT-401461.
STRINGi 4932.YCL040W.

Proteomic databases

MaxQBi P17709.
PaxDbi P17709.
PeptideAtlasi P17709.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCL040W ; YCL040W ; YCL040W .
GeneIDi 850317.
KEGGi sce:YCL040W.

Organism-specific databases

CYGDi YCL040w.
SGDi S000000545. GLK1.

Phylogenomic databases

eggNOGi COG5026.
GeneTreei ENSGT00390000017159.
HOGENOMi HOG000162670.
KOi K00844.
OMAi GEVEIGC.
OrthoDBi EOG79SF68.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-5982.
YEAST:YCL040W-MONOMER.
SABIO-RK P17709.

Miscellaneous databases

NextBioi 965721.

Gene expression databases

Genevestigatori P17709.

Family and domain databases

InterProi IPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR019807. Hexokinase_CS.
IPR022672. Hexokinase_N.
[Graphical view ]
PANTHERi PTHR19443. PTHR19443. 1 hit.
Pfami PF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view ]
PRINTSi PR00475. HEXOKINASE.
PROSITEi PS00378. HEXOKINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of yeast glucokinase, a strongly diverged specific aldo-hexose-phosphorylating isoenzyme."
    Albig W., Entian K.-D.
    Gene 73:141-152(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete sequence of a 9,543 bp segment on the left arm of chromosome III reveals five open reading frames including glucokinase and the protein disulfide isomerase."
    Scherens B., Messenguy F., Gigot D., Dubois E.
    Yeast 8:577-586(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-470, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHXKG_YEAST
AccessioniPrimary (citable) accession number: P17709
Secondary accession number(s): D6VQX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 11, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 21100 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi