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Protein

Glucokinase-1

Gene

GLK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Two isoenzymes, hexokinase-1 and hexokinase-2, can phosphorylate keto- and aldohexoses in yeast, whereas a third isoenzyme, GLK, is specific for aldohexoses. All glucose phosphorylating enzymes are involved in glucose uptake.

Miscellaneous

Present with 21100 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + D-glucose = ADP + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110ATPSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi487 – 492ATPSequence analysis6

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • fructokinase activity Source: GO_Central
  • glucokinase activity Source: SGD
  • glucose binding Source: InterPro
  • mannokinase activity Source: GO_Central

GO - Biological processi

  • cellular glucose homeostasis Source: GO_Central
  • glucose import Source: SGD
  • glucose metabolic process Source: SGD
  • glycolytic process Source: SGD
  • mannose metabolic process Source: SGD

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YCL040W-MONOMER
YEAST:YCL040W-MONOMER
BRENDAi2.7.1.2 984
SABIO-RKP17709

Names & Taxonomyi

Protein namesi
Recommended name:
Glucokinase-1 (EC:2.7.1.2)
Alternative name(s):
Glucose kinase 1
Short name:
GLK-1
Gene namesi
Name:GLK1
Synonyms:HOR3
Ordered Locus Names:YCL040W
ORF Names:YCL312, YCL40W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCL040W
SGDiS000000545 GLK1

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001976032 – 500Glucokinase-1Add BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1
Modified residuei470PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP17709
PaxDbiP17709
PRIDEiP17709

PTM databases

iPTMnetiP17709

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi30943, 56 interactors
DIPiDIP-525N
IntActiP17709, 56 interactors
MINTiP17709
STRINGi4932.YCL040W

Structurei

3D structure databases

ProteinModelPortaliP17709
SMRiP17709
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 498HexokinasePROSITE-ProRule annotationAdd BLAST487

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni74 – 216Hexokinase small subdomainPROSITE-ProRule annotationAdd BLAST143
Regioni158 – 184Glucose-bindingSequence analysisAdd BLAST27
Regioni217 – 487Hexokinase large subdomainPROSITE-ProRule annotationAdd BLAST271

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated

Phylogenomic databases

GeneTreeiENSGT00910000145864
HOGENOMiHOG000162670
InParanoidiP17709
KOiK00844
OMAiMIPTFVT
OrthoDBiEOG092C2JW4

Family and domain databases

InterProiView protein in InterPro
IPR001312 Hexokinase
IPR019807 Hexokinase_BS
IPR022673 Hexokinase_C
IPR022672 Hexokinase_N
PANTHERiPTHR19443 PTHR19443, 1 hit
PfamiView protein in Pfam
PF00349 Hexokinase_1, 1 hit
PF03727 Hexokinase_2, 1 hit
PROSITEiView protein in PROSITE
PS00378 HEXOKINASE_1, 1 hit
PS51748 HEXOKINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17709-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFDDLHKAT ERAVIQAVDQ ICDDFEVTPE KLDELTAYFI EQMEKGLAPP
60 70 80 90 100
KEGHTLASDK GLPMIPAFVT GSPNGTERGV LLAADLGGTN FRICSVNLHG
110 120 130 140 150
DHTFSMEQMK SKIPDDLLDD ENVTSDDLFG FLARRTLAFM KKYHPDELAK
160 170 180 190 200
GKDAKPMKLG FTFSYPVDQT SLNSGTLIRW TKGFRIADTV GKDVVQLYQE
210 220 230 240 250
QLSAQGMPMI KVVALTNDTV GTYLSHCYTS DNTDSMTSGE ISEPVIGCIF
260 270 280 290 300
GTGTNGCYME EINKITKLPQ ELRDKLIKEG KTHMIINVEW GSFDNELKHL
310 320 330 340 350
PTTKYDVVID QKLSTNPGFH LFEKRVSGMF LGEVLRNILV DLHSQGLLLQ
360 370 380 390 400
QYRSKEQLPR HLTTPFQLSS EVLSHIEIDD STGLRETELS LLQSLRLPTT
410 420 430 440 450
PTERVQIQKL VRAISRRSAY LAAVPLAAIL IKTNALNKRY HGEVEIGCDG
460 470 480 490 500
SVVEYYPGFR SMLRHALALS PLGAEGERKV HLKIAKDGSG VGAALCALVA
Length:500
Mass (Da):55,377
Last modified:August 1, 1990 - v1
Checksum:iA7A417041D7A961F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24077 Genomic DNA Translation: AAA53536.1
X59720 Genomic DNA Translation: CAA42376.1
BK006937 Genomic DNA Translation: DAA07444.1
PIRiJT0482
RefSeqiNP_009890.1, NM_001178685.1

Genome annotation databases

EnsemblFungiiCAA42376; CAA42376; CAA42376
YCL040W; YCL040W; YCL040W
GeneIDi850317
KEGGisce:YCL040W

Similar proteinsi

Entry informationi

Entry nameiHXKG_YEAST
AccessioniPrimary (citable) accession number: P17709
Secondary accession number(s): D6VQX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 23, 2018
This is version 168 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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