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P17706 (PTN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein phosphatase non-receptor type 2
EC=3.1.3.48
Alternative name(s):
T-cell protein-tyrosine phosphatase
Short name=TCPTP
Gene names
Name:PTPN2
Synonyms:PTPT
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Interacts with RMDN3. Isoform PTPB interacts with TMED9. Ref.9 Ref.10

Subcellular location

Cytoplasm Ref.8.

Isoform PTPB: Endoplasmic reticulum Ref.8.

Isoform PTPA: Nucleus Ref.8.

Tissue specificity

Isoform PTPA is probably the major isoform. Isoform PTPB is expressed in T-cells and in placenta.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.

Contains 1 tyrosine-protein phosphatase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GHRP109128EBI-984930,EBI-286316
TMED10P497555EBI-4409481,EBI-998422
TMED9Q9BVK65EBI-4409481,EBI-1056827

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform PTPB (identifier: P17706-1)

Also known as: p48TC; TC48;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform PTPA (identifier: P17706-2)

Also known as: p45TC; TC45;

The sequence of this isoform differs from the canonical sequence as follows:
     382-415: WLYWQPILTKMGFMSVILVGAFVGWTLFFQQNAL → PRLTDT
Isoform 3 (identifier: P17706-3)

The sequence of this isoform differs from the canonical sequence as follows:
     347-380: Missing.
     382-415: WLYWQPILTKMGFMSVILVGAFVGWTLFFQQNAL → PRLTDT
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415Tyrosine-protein phosphatase non-receptor type 2
PRO_0000094752

Regions

Domain5 – 275271Tyrosine-protein phosphatase
Region216 – 2227Substrate binding By similarity

Sites

Active site2161Phosphocysteine intermediate By similarity
Binding site1821Substrate By similarity
Binding site2601Substrate By similarity

Amino acid modifications

Modified residue2981Phosphoserine Ref.11
Modified residue3041Phosphoserine Ref.11 Ref.12 Ref.14

Natural variations

Alternative sequence347 – 38034Missing in isoform 3.
VSP_043383
Alternative sequence382 – 41534WLYWQ…QQNAL → PRLTDT in isoform PTPA and isoform 3.
VSP_005125

Experimental info

Sequence conflict4071T → R in AAA65997. Ref.1
Sequence conflict4071T → R in M81478. Ref.2

Secondary structure

1 ............................................... 415
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform PTPB (p48TC) (TC48) [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 0207694A4F058E68

FASTA41548,473
        10         20         30         40         50         60 
MPTTIEREFE ELDTQRRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK 

        70         80         90        100        110        120 
LQNAENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTKAVV MLNRIVEKES 

       130        140        150        160        170        180 
VKCAQYWPTD DQEMLFKETG FSVKLLSEDV KSYYTVHLLQ LENINSGETR TISHFHYTTW 

       190        200        210        220        230        240 
PDFGVPESPA SFLNFLFKVR ESGSLNPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGD 

       250        260        270        280        290        300 
DINIKQVLLN MRKYRMGLIQ TPDQLRFSYM AIIEGAKCIK GDSSIQKRWK ELSKEDLSPA 

       310        320        330        340        350        360 
FDHSPNKIMT EKYNGNRIGL EEEKLTGDRC TGLSSKMQDT MEENSESALR KRIREDRKAT 

       370        380        390        400        410 
TAQKVQQMKQ RLNENERKRK RWLYWQPILT KMGFMSVILV GAFVGWTLFF QQNAL

« Hide

Isoform PTPA (p45TC) (TC45) [UniParc].

Checksum: 0F3E622EBA5A92CB
Show »

FASTA38745,168
Isoform 3 [UniParc].

Checksum: 5AE668EB7D76CB93
Show »

FASTA35340,990

References

« Hide 'large scale' references
[1]"cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family."
Cool D., Tonks N.K., Charbonneau H., Walsh K.A., Fischer E.H., Krebs E.G.
Proc. Natl. Acad. Sci. U.S.A. 86:5257-5261(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: T-cell.
[2]"Cloning and characterization of a mouse cDNA encoding a cytoplasmic protein-tyrosine-phosphatase."
Mosinger B. Jr., Tillmann U., Westphal H., Tremblay M.L.
Proc. Natl. Acad. Sci. U.S.A. 89:499-503(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]NHLBI resequencing and genotyping service (RS&G)
Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[5]"DNA sequence and analysis of human chromosome 18."
Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. expand/collapse author list , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.
Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS PTPB AND 3).
Tissue: Brain and Eye.
[8]"COOH-terminal sequence motifs target the T cell protein tyrosine phosphatase to the ER and nucleus."
Lorenzen J.A., Dadabay C.Y., Fischer E.H.
J. Cell Biol. 131:631-643(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION (ISOFORMS PTPA AND PTPB).
[9]"The novel protein PTPIP51 exhibits tissue- and cell-specific expression."
Stenzinger A., Kajosch T., Tag C., Porsche A., Welte I., Hofer H.W., Steger K., Wimmer M.
Histochem. Cell Biol. 123:19-28(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RMDN3.
[10]"Evidence for a role of transmembrane protein p25 in localization of protein tyrosine phosphatase TC48 to the ER."
Gupta V., Swarup G.
J. Cell Sci. 119:1703-1714(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TMED9.
[11]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-298 AND SER-304, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304, MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304, MASS SPECTROMETRY.
[15]"Structure determination of T cell protein-tyrosine phosphatase."
Iversen L.F., Moller K.B., Pedersen A.K., Peters G.H., Petersen A.S., Andersen H.S., Branner S., Mortensen S.B., Moller N.P.
J. Biol. Chem. 277:19982-19990(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS) OF 1-314.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M25393 mRNA. Translation: AAA65997.1.
M81478 mRNA. No translation available.
EF445017 Genomic DNA. Translation: ACA06062.1.
EF445017 Genomic DNA. Translation: ACA06064.1.
AK292570 mRNA. Translation: BAF85259.1.
AP001077 Genomic DNA. No translation available.
AP002449 Genomic DNA. No translation available.
AP005482 Genomic DNA. No translation available.
CH471113 Genomic DNA. Translation: EAX01532.1.
CH471113 Genomic DNA. Translation: EAX01539.1.
BC008244 mRNA. Translation: AAH08244.1.
BC016727 mRNA. Translation: AAH16727.1.
IPIIPI00106928.
IPI00218788.
IPI00219695.
PIRA33899.
RefSeqNP_002819.2. NM_002828.3.
NP_536347.1. NM_080422.2.
NP_536348.1. NM_080423.2.
UniGeneHs.654527.
Hs.663373.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1L8KX-ray2.56A1-314[»]
ProteinModelPortalP17706.
ModBaseSearch...

Protein-protein interaction databases

IntActP17706. 10 interactions.
MINTMINT-3975566.
STRING9606.ENSP00000311857.

PTM databases

PhosphoSiteP17706.

Polymorphism databases

DMDM229462762.

Proteomic databases

PaxDbP17706.
PRIDEP17706.

Protocols and materials databases

DNASU5771.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000309660; ENSP00000311857; ENSG00000175354.
ENST00000327283; ENSP00000320298; ENSG00000175354.
ENST00000353319; ENSP00000320546; ENSG00000175354.
GeneID5771.
KEGGhsa:5771.
UCSCuc002krp.3. human.

Organism-specific databases

CTD5771.
GeneCardsGC18M012775.
HGNCHGNC:9650. PTPN2.
HPAHPA015004.
HPA046176.
MIM176887. gene.
neXtProtNX_P17706.
PharmGKBPA33993.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5599.
HOGENOMHOG000273908.
HOVERGENHBG008321.
InParanoidP17706.
KOK01104.
OMANTAQKVQ.
PhylomeDBP17706.

Enzyme and pathway databases

Pathway_Interaction_DBifngpathway. IFN-gamma pathway.
pdgfrbpathway. PDGFR-beta signaling pathway.
ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressP17706.
BgeeP17706.
CleanExHS_PTPN2.
GenevestigatorP17706.
GermOnlineENSG00000175354. Homo sapiens.

Family and domain databases

InterProIPR012265. Ptpn1/2.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PANTHERPTHR19134:SF57. PTHR19134:SF57. 1 hit.
PfamPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFPIRSF000926. Tyr-Ptase_nr1. 1 hit.
PRINTSPR00700. PRTYPHPHTASE.
SMARTSM00194. PTPc. 1 hit.
[Graphical view]
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP17706.
ChEMBLCHEMBL3807.
ChiTaRSPTPN2. human.
EvolutionaryTraceP17706.
GenomeRNAi5771.
NextBio22446.
SOURCESearch...

Entry information

Entry namePTN2_HUMAN
AccessionPrimary (citable) accession number: P17706
Secondary accession number(s): A8K955 expand/collapse secondary AC list , A8MXU3, Q96AU5, Q96HR2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 5, 2009
Last modified: May 1, 2013
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents