Reviewed, UniProtKB/Swiss-Prot P17706 (PTN2_HUMAN)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein phosphatase non-receptor type 2
    EC=3.1.3.48
Alternative name(s):
    T-cell protein-tyrosine phosphatase
      Short name=TCPTP

Gene names

Name:	PTPN2
Synonyms:	PTPT

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit structure	Interacts with FAM82A2. Ref.7
Subcellular location	Cytoplasm.
Tissue specificity	PTPA isoform is probably the major PTP expressed in human tissues. PTPB isoform was found in T-cells and in placenta.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein amino acid dephosphorylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein tyrosine phosphatase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GHR	P10912	2	EBI-984930,EBI-286316
WASL	O00401	1	EBI-984930,EBI-957615

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform PTPB (identifier: P17706-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform PTPA (identifier: P17706-2)

The sequence of this isoform differs from the canonical sequence as follows:
     382-415: WLYWQPILTKMGFMSVILVGAFVGWTLFFQQNAL → PRLTDT

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 415

415

Tyrosine-protein phosphatase non-receptor type 2

PRO_0000094752

Regions

Domain

5 – 275

271

Tyrosine-protein phosphatase

Sites

Active site

216

1

Phosphocysteine intermediate By similarity

Amino acid modifications

Modified residue

298

1

Phosphoserine Ref.8

Modified residue

304

1

Phosphoserine Ref.8

Natural variations

Alternative sequence

382 – 415

34

WLYWQ…QQNAL → PRLTDT in isoform PTPA.

VSP_005125

Experimental info

Sequence conflict

407

1

T → R in AAA65997. Ref.1

Sequence conflict

407

1

T → R in M81478. Ref.2

Secondary structure

1

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415

Helix Strand Turn

Details...

Helix

7 – 14

8

Helix

19 – 28

10

Helix

35 – 38

4

Helix

40 – 45

6

Turn

55 – 57

3

Beta strand

58 – 60

3

Beta strand

68 – 76

9

Helix

77 – 79

3

Beta strand

81 – 87

7

Turn

91 – 93

3

Helix

94 – 103

10

Beta strand

108 – 111

4

Beta strand

129 – 132

4

Beta strand

134 – 136

3

Turn

137 – 140

4

Beta strand

141 – 150

10

Beta strand

152 – 163

12

Turn

164 – 166

3

Beta strand

169 – 177

9

Beta strand

182 – 184

3

Helix

190 – 201

12

Turn

202 – 205

4

Beta strand

212 – 221

10

Helix

222 – 235

14

Beta strand

236 – 239

4

Helix

244 – 251

8

Turn

252 – 254

3

Helix

262 – 275

14

Sequences

	Sequence		Length	Mass (Da)
	Isoform PTPB [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 0207694A4F058E68	FASTA	415	48,473
	10 20 30 40 50 60 MPTTIEREFE ELDTQRRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK 70 80 90 100 110 120 LQNAENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTKAVV MLNRIVEKES 130 140 150 160 170 180 VKCAQYWPTD DQEMLFKETG FSVKLLSEDV KSYYTVHLLQ LENINSGETR TISHFHYTTW 190 200 210 220 230 240 PDFGVPESPA SFLNFLFKVR ESGSLNPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGD 250 260 270 280 290 300 DINIKQVLLN MRKYRMGLIQ TPDQLRFSYM AIIEGAKCIK GDSSIQKRWK ELSKEDLSPA 310 320 330 340 350 360 FDHSPNKIMT EKYNGNRIGL EEEKLTGDRC TGLSSKMQDT MEENSESALR KRIREDRKAT 370 380 390 400 410 TAQKVQQMKQ RLNENERKRK RWLYWQPILT KMGFMSVILV GAFVGWTLFF QQNAL
	Isoform PTPA. Checksum: 0F3E622EBA5A92CB	FASTA	387	45,168
	10 20 30 40 50 60 MPTTIEREFE ELDTQRRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK 70 80 90 100 110 120 LQNAENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTKAVV MLNRIVEKES 130 140 150 160 170 180 VKCAQYWPTD DQEMLFKETG FSVKLLSEDV KSYYTVHLLQ LENINSGETR TISHFHYTTW 190 200 210 220 230 240 PDFGVPESPA SFLNFLFKVR ESGSLNPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGD 250 260 270 280 290 300 DINIKQVLLN MRKYRMGLIQ TPDQLRFSYM AIIEGAKCIK GDSSIQKRWK ELSKEDLSPA 310 320 330 340 350 360 FDHSPNKIMT EKYNGNRIGL EEEKLTGDRC TGLSSKMQDT MEENSESALR KRIREDRKAT 370 380 TAQKVQQMKQ RLNENERKRK RPRLTDT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family." Cool D., Tonks N.K., Charbonneau H., Walsh K.A., Fischer E.H., Krebs E.G. Proc. Natl. Acad. Sci. U.S.A. 86:5257-5261(1989) [PubMed: 2546150] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: T-cell.
[2]	"Cloning and characterization of a mouse cDNA encoding a cytoplasmic protein-tyrosine-phosphatase." Mosinger B. Jr., Tillmann U., Westphal H., Tremblay M.L. Proc. Natl. Acad. Sci. U.S.A. 89:499-503(1992) [PubMed: 1731319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	NHLBI resequencing and genotyping service (RS&G) Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM PTPB). Tissue: Eye.
[7]	"The novel protein PTPIP51 exhibits tissue- and cell-specific expression." Stenzinger A., Kajosch T., Tag C., Porsche A., Welte I., Hofer H.W., Steger K., Wimmer M. Histochem. Cell Biol. 123:19-28(2005) [PubMed: 15609043] [Abstract] Cited for: INTERACTION WITH FAM82A2.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-298 AND SER-304, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]	"Structure determination of T cell protein-tyrosine phosphatase." Iversen L.F., Moller K.B., Pedersen A.K., Peters G.H., Petersen A.S., Andersen H.S., Branner S., Mortensen S.B., Moller N.P. J. Biol. Chem. 277:19982-19990(2002) [PubMed: 11907034] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.56 ANGSTROMS) OF 1-314.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M25393 mRNA. Translation: AAA65997.1.
M81478 mRNA. No translation available.
EF445017 Genomic DNA. Translation: ACA06062.1.
AK292570 mRNA. Translation: BAF85259.1.
CH471113 Genomic DNA. Translation: EAX01532.1.
BC008244 mRNA. Translation: AAH08244.1.

IPI

IPI00218788.
IPI00219695.

PIR

A33899.

RefSeq

NP_002819.1.
NP_536347.1.

UniGene

Hs.654527

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1L8K	X-ray	2.56	A	1-314	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P17706. 3 interactions.

PTM databases

PhosphoSite

P17706.

Genome annotation databases

Ensembl

ENSG00000175354. Homo sapiens. [Contig view]

GeneID

5771.

KEGG

hsa:5771.

Organism-specific databases

GeneCards

GC18M012775.

H-InvDB

HIX0014345.

HGNC

HGNC:9650. PTPN2.

MIM

176887. gene.

PharmGKB

PA33993.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P17706.

HOVERGEN

P17706.

Enzyme and pathway databases

BRENDA

3.1.3.48. 247.

Pathway_Interaction_DB

ifngpathway. IFN-gamma pathway.
pdgfrbpathway. PDGFR-beta signaling pathway.

Gene expression databases

ArrayExpress

P17706.

Bgee

P17706.

CleanEx

HS_PTPN2.

GermOnline

ENSG00000175354. Homo sapiens.

Family and domain databases

InterPro

IPR000387. Tyr_Pase.
IPR016130. Tyr_Pase_AS.
IPR012265. Tyr_Pase_non-rcpt_typ-1/2.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]

PANTHER

PTHR19134:SF57. Tyr_Phos_no_rcpt. 1 hit.

Pfam

PF00102. Y_phosphatase. 1 hit.
[Graphical view]

PIRSF

PIRSF000926. Tyr-Ptase_nr1. 1 hit.

PRINTS

PR00700. PRTYPHPHTASE.

SMART

SM00194. PTPc. 1 hit.
[Graphical view]

PROSITE

PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

BindingDB

P17706.

NextBio

22446.

SOURCE

Search...

Entry information

Entry name

PTN2_HUMAN

Accession

Primary (citable) accession number: P17706
Secondary accession number(s): A8K955, Q96HR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	May 5, 2009
Last modified:	June 16, 2009
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)