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Protein

Outer capsid glycoprotein VP7

Gene
N/A
Organism
Rotavirus A (isolate Cow/United States/B223/1983 G10-P8[11]-I2-Rx-Cx-Mx-A13-Nx-Tx-E2-Hx) (RV-A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved (By similarity).By similarity

GO - Biological processi

  1. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid glycoprotein VP7
OrganismiRotavirus A (isolate Cow/United States/B223/1983 G10-P8[11]-I2-Rx-Cx-Mx-A13-Nx-Tx-E2-Hx) (RV-A)
Taxonomic identifieri10930 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]

Subcellular locationi

  1. Virion By similarity
  2. Host endoplasmic reticulum lumen Curated

  3. Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles (By similarity).By similarity

GO - Cellular componenti

  1. host cell endoplasmic reticulum lumen Source: UniProtKB-SubCell
  2. T=13 icosahedral viral capsid Source: UniProtKB-KW
  3. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5050Sequence AnalysisAdd
BLAST
Chaini51 – 326276Outer capsid glycoprotein VP7Sequence AnalysisPRO_0000149582Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi145 – 1451N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

The N-terminus is blocked possibly by pyroglutamic acid.By similarity
N-glycosylated.By similarity
Intramolecular disulfide bonds.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; in the presence of calcium (By similarity). Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP7 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001963. VP7.
[Graphical view]
PfamiPF00434. VP7. 1 hit.
[Graphical view]
ProDomiPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P17700-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYGIEYTTFL IYLISIILLN YILKSITRMM DYIIYKFLLI VTITSIVVNA
60 70 80 90 100
QNYGINLPIT GSMDTSYVNA TKDEPFLTST LCLYYPTEAR TEINDNEWTS
110 120 130 140 150
TLSQLFLTKG WPTGSVYFKE YDDIATFSVD PQLYCDYNIV LMRYNSSLKL
160 170 180 190 200
DMSELANLIL NEWLSNPMDI TLYYYQQTDE ANKWIAMGQS CTIKVCPLNT
210 220 230 240 250
QTLGIGCQTT NTGTFEEVAT AEKLVITDVV DGVNHKLDVT TATCTIRNCK
260 270 280 290 300
KLGPRENVAV IQVGGADILD ITSDPTTNPQ TERMMRINWK KWWQVFYTIV
310 320
DYVNQIVQAM SKRSRSLNSA AFYYRV
Length:326
Mass (Da):37,310
Last modified:August 1, 1990 - v1
Checksum:iD9253D69DD2CE2EE
GO
Isoform 2 (identifier: P17700-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Note: Produced by alternative initiation at Met-30 of isoform 1. No experimental confirmation available.

Show »
Length:297
Mass (Da):33,828
Checksum:i63EEDAD2A193C05D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81T → A in CAA40982 (Ref. 2) Curated
Sequence conflicti65 – 651T → S in CAA40982 (Ref. 2) Curated
Sequence conflicti149 – 1491K → E in CAA40982 (Ref. 2) Curated
Sequence conflicti165 – 1651S → C in CAA40982 (Ref. 2) Curated
Sequence conflicti178 – 1792TD → NG in CAA40982 (Ref. 2) Curated
Sequence conflicti213 – 2131G → R in CAA40982 (Ref. 2) Curated
Sequence conflicti222 – 2221E → Q in CAA40982 (Ref. 2) Curated
Sequence conflicti225 – 2251V → P in CAA40982 (Ref. 2) Curated
Sequence conflicti231 – 2311D → V in CAA40982 (Ref. 2) Curated
Sequence conflicti284 – 2841M → I in CAA40982 (Ref. 2) Curated
Sequence conflicti295 – 2951V → A in CAA40982 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2929Missing in isoform 2. CuratedVSP_038586Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52650 Genomic RNA. Translation: CAA36875.1.
X57852 mRNA. Translation: CAA40982.1.
PIRiS29562.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52650 Genomic RNA. Translation: CAA36875.1.
X57852 mRNA. Translation: CAA40982.1.
PIRiS29562.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001963. VP7.
[Graphical view]
PfamiPF00434. VP7. 1 hit.
[Graphical view]
ProDomiPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of the gene encoding the major neutralization antigen (VP7) of serotype 10 rotavirus."
    Xu L.I., Harbour D.A., McCrae M.A.
    J. Gen. Virol. 72:177-180(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Nucleotide sequence of the VP7 gene of serotype 10 bovine rotavirus strain B223."
    Hardy M.E., Woode G.N.
    Submitted (FEB-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP7_ROTBB
AccessioniPrimary (citable) accession number: P17700
Secondary accession number(s): Q65702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.