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P17694 (NDUS2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
Complex I-49kD
Short name=CI-49kD
NADH-ubiquinone oxidoreductase 49 kDa subunit
Gene names
Name:NDUFS2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. HAMAP-Rule MF_01358

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01358

NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01358

Cofactor

Binds 1 4Fe-4S cluster.

Subunit structure

Complex I is composed of 45 different subunits. Interacts with NDUFAF3 and NDUFAF7 By similarity. Component of the iron-sulfur (IP) fragment of the enzyme.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side HAMAP-Rule MF_01358.

Sequence similarities

Belongs to the complex I 49 kDa subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3333Mitochondrion Ref.2
Chain34 – 463430NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial HAMAP-Rule MF_01358
PRO_0000118582

Sites

Metal binding3261Iron-sulfur (4Fe-4S) Potential
Metal binding3321Iron-sulfur (4Fe-4S) Potential
Metal binding3471Iron-sulfur (4Fe-4S) Potential

Amino acid modifications

Modified residue621N6-acetyllysine By similarity
Modified residue1181Symmetric dimethylarginine Ref.3

Experimental info

Sequence conflict1621Q → R in CAA32523. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P17694 [UniParc].

Last modified October 17, 2006. Version 2.
Checksum: 7EE8B22C289EA76F

FASTA46352,556
        10         20         30         40         50         60 
MAALRALCRL RGAAAQVLRP GAGVRLPIQP SRGARQWQPD VEWAEQYGGA VMYPTKETAH 

        70         80         90        100        110        120 
WKPPPWNDVD PPKDTLVSNL TLNFGPQHPA AHGVLRLVME LSGEMVRKCD PHIGLLHRGT 

       130        140        150        160        170        180 
EKLIEYKTYL QALPYFDRLD YVSMMCNEQA YSLAVEKLLN IQPPPRAQWI RVLFGEITRL 

       190        200        210        220        230        240 
LNHIMAVTTH ALDIGAMTPF FWMFEEREKM FEFYERVSGA RMHAAYVRPG GVHQDLPLGL 

       250        260        270        280        290        300 
MDDIYEFSKN FSLRIDELEE MLTNNRIWRN RTVDIGIVTA EDALNYGFSG VMLRGSGIQW 

       310        320        330        340        350        360 
DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS IRIISQCLNK MPPGEIKVDD 

       370        380        390        400        410        420 
AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI EAPKGEFGVY LVSDGSSRPY 

       430        440        450        460 
RCKIKAPGFA HLAGLDKMSK GHMLADVVAI IGTQDIVFGE VDR 

« Hide

References

« Hide 'large scale' references
[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal muscle.
[2]"A homologue of the nuclear coded 49 kd subunit of bovine mitochondrial NADH-ubiquinone reductase is coded in chloroplast DNA."
Fearnley I.M., Runswick M.J., Walker J.E.
EMBO J. 8:665-672(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-463, PROTEIN SEQUENCE OF 34-55.
Tissue: Heart.
[3]"Post-translational modifications near the quinone binding site of mammalian complex I."
Carroll J., Ding S., Fearnley I.M., Walker J.E.
J. Biol. Chem. 288:24799-24808(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT ARG-118.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC119924 mRNA. Translation: AAI19925.1.
X14338 mRNA. Translation: CAA32523.1.
PIRS04104.
RefSeqNP_001068605.1. NM_001075137.1.
UniGeneBt.4475.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP17694. 3 interactions.
STRING9913.ENSBTAP00000002852.

Protein family/group databases

TCDB3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PRIDEP17694.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000002852; ENSBTAP00000002852; ENSBTAG00000002203.
GeneID327697.
KEGGbta:327697.

Organism-specific databases

CTD4720.

Phylogenomic databases

eggNOGCOG0649.
GeneTreeENSGT00390000009529.
HOGENOMHOG000228264.
HOVERGENHBG000760.
InParanoidP17694.
KOK03935.
OMAEYRTWTQ.
OrthoDBEOG7WT411.
TreeFamTF300370.

Family and domain databases

HAMAPMF_01358. NDH1_NuoD.
InterProIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
[Graphical view]
PfamPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
TIGRFAMsTIGR01962. NuoD. 1 hit.
PROSITEPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20810161.

Entry information

Entry nameNDUS2_BOVIN
AccessionPrimary (citable) accession number: P17694
Secondary accession number(s): Q0P5K5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families