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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Gene

NDUFS2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.2 Publications

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi326Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi332Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi347Iron-sulfur (4Fe-4S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BRENDAi1.6.5.3 908
ReactomeiR-BTA-611105 Respiratory electron transport
R-BTA-6799198 Complex I biogenesis

Protein family/group databases

TCDBi3.D.1.6.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-49kD
Short name:
CI-49kD
NADH-ubiquinone oxidoreductase 49 kDa subunit
Gene namesi
Name:NDUFS2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Organism-specific databases

VGNCiVGNC:31969 NDUFS2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33Mitochondrion1 PublicationAdd BLAST33
ChainiPRO_000011858234 – 463NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialAdd BLAST430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei62N6-acetyllysineBy similarity1
Modified residuei118Symmetric dimethylarginine1 Publication1

Post-translational modificationi

Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex.By similarity

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

PaxDbiP17694
PRIDEiP17694

Expressioni

Gene expression databases

BgeeiENSBTAG00000002203

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits (PubMed:10852722, PubMed:18721790). Component of the iron-sulfur (IP) fragment of the enzyme (PubMed:10852722, PubMed:18721790). Interacts with NDUFAF3 (By similarity). Interacts with NDUFAF7 (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP17694
DIPiDIP-38816N
IntActiP17694, 3 interactors
STRINGi9913.ENSBTAP00000002852

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LC5electron microscopy4.35D38-463[»]
5LDWelectron microscopy4.27D35-463[»]
5LDXelectron microscopy5.60D35-463[»]
5O31electron microscopy4.13D34-463[»]
SMRiP17694
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2870 Eukaryota
COG0649 LUCA
GeneTreeiENSGT00390000009529
HOGENOMiHOG000228264
HOVERGENiHBG000760
InParanoidiP17694
KOiK03935
OMAiKKIAWPA
OrthoDBiEOG091G0WWQ
TreeFamiTF300370

Family and domain databases

Gene3Di1.10.645.20, 1 hit
HAMAPiMF_01358 NDH1_NuoD, 1 hit
InterProiView protein in InterPro
IPR001135 NADH_Q_OxRdtase_suD
IPR038290 NADH_Q_OxRdtase_suD_sf
IPR014029 NADH_UbQ_OxRdtase_49kDa_CS
IPR022885 NDH1_su_D/H
IPR029014 NiFe-Hase_large
PfamiView protein in Pfam
PF00346 Complex1_49kDa, 1 hit
SUPFAMiSSF56762 SSF56762, 1 hit
TIGRFAMsiTIGR01962 NuoD, 1 hit
PROSITEiView protein in PROSITE
PS00535 COMPLEX1_49K, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17694-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALRALCRL RGAAAQVLRP GAGVRLPIQP SRGARQWQPD VEWAEQYGGA
60 70 80 90 100
VMYPTKETAH WKPPPWNDVD PPKDTLVSNL TLNFGPQHPA AHGVLRLVME
110 120 130 140 150
LSGEMVRKCD PHIGLLHRGT EKLIEYKTYL QALPYFDRLD YVSMMCNEQA
160 170 180 190 200
YSLAVEKLLN IQPPPRAQWI RVLFGEITRL LNHIMAVTTH ALDIGAMTPF
210 220 230 240 250
FWMFEEREKM FEFYERVSGA RMHAAYVRPG GVHQDLPLGL MDDIYEFSKN
260 270 280 290 300
FSLRIDELEE MLTNNRIWRN RTVDIGIVTA EDALNYGFSG VMLRGSGIQW
310 320 330 340 350
DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS IRIISQCLNK
360 370 380 390 400
MPPGEIKVDD AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI
410 420 430 440 450
EAPKGEFGVY LVSDGSSRPY RCKIKAPGFA HLAGLDKMSK GHMLADVVAI
460
IGTQDIVFGE VDR
Length:463
Mass (Da):52,556
Last modified:October 17, 2006 - v2
Checksum:i7EE8B22C289EA76F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162Q → R in CAA32523 (PubMed:2498081).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC119924 mRNA Translation: AAI19925.1
X14338 mRNA Translation: CAA32523.1
PIRiS04104
RefSeqiNP_001068605.1, NM_001075137.1
UniGeneiBt.4475

Genome annotation databases

EnsembliENSBTAT00000002852; ENSBTAP00000002852; ENSBTAG00000002203
GeneIDi327697
KEGGibta:327697

Similar proteinsi

Entry informationi

Entry nameiNDUS2_BOVIN
AccessioniPrimary (citable) accession number: P17694
Secondary accession number(s): Q0P5K5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 17, 2006
Last modified: March 28, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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