NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial precursor

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P17694 (NDUS2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-49kD
Short name=CI-49kD
NADH-ubiquinone oxidoreductase 49 kDa subunit

Gene names

Name:

NDUFS2

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster.
Subunit structure	Complex I is composed of 45 different subunits. Interacts with NDUFAF3 and NDUFAF7 By similarity. Component of the iron-sulfur (IP) fragment of the enzyme.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from direct assay PubMed 12857734. Source: UniProtKB
Cellular_component	mitochondrial respiratory chain complex I Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 33

Mitochondrion Ref.2

Chain

34 – 463

430

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

PRO_0000118582

Sites

Metal binding

326

Iron-sulfur (4Fe-4S) Potential

Metal binding

332

Iron-sulfur (4Fe-4S) Potential

Metal binding

347

Iron-sulfur (4Fe-4S) Potential

Experimental info

Sequence conflict

162

Q → R in CAA32523. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P17694 [UniParc].

Last modified October 17, 2006. Version 2.
Checksum: 7EE8B22C289EA76F
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FASTA

463

52,556

        10         20         30         40         50         60 
MAALRALCRL RGAAAQVLRP GAGVRLPIQP SRGARQWQPD VEWAEQYGGA VMYPTKETAH 

        70         80         90        100        110        120 
WKPPPWNDVD PPKDTLVSNL TLNFGPQHPA AHGVLRLVME LSGEMVRKCD PHIGLLHRGT 

       130        140        150        160        170        180 
EKLIEYKTYL QALPYFDRLD YVSMMCNEQA YSLAVEKLLN IQPPPRAQWI RVLFGEITRL 

       190        200        210        220        230        240 
LNHIMAVTTH ALDIGAMTPF FWMFEEREKM FEFYERVSGA RMHAAYVRPG GVHQDLPLGL 

       250        260        270        280        290        300 
MDDIYEFSKN FSLRIDELEE MLTNNRIWRN RTVDIGIVTA EDALNYGFSG VMLRGSGIQW 

       310        320        330        340        350        360 
DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS IRIISQCLNK MPPGEIKVDD 

       370        380        390        400        410        420 
AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI EAPKGEFGVY LVSDGSSRPY 

       430        440        450        460 
RCKIKAPGFA HLAGLDKMSK GHMLADVVAI IGTQDIVFGE VDR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal muscle.
[2]	"A homologue of the nuclear coded 49 kd subunit of bovine mitochondrial NADH-ubiquinone reductase is coded in chloroplast DNA." Fearnley I.M., Runswick M.J., Walker J.E. EMBO J. 8:665-672(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-463, PROTEIN SEQUENCE OF 34-55. Tissue: Heart.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC119924 mRNA. Translation: AAI19925.1. X14338 mRNA. Translation: CAA32523.1.
IPI	IPI00708720.
PIR	S04104.
RefSeq	NP_001068605.1. NM_001075137.1.
UniGene	Bt.4475.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P17694. 3 interactions.
STRING	9913.ENSBTAP00000002852.
Protein family/group databases
TCDB	3.D.1.6.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Proteomic databases
PRIDE	P17694.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000002852; ENSBTAP00000002852; ENSBTAG00000002203.
GeneID	327697.
KEGG	bta:327697.
Organism-specific databases
CTD	4720.
Phylogenomic databases
eggNOG	COG0649.
GeneTree	ENSGT00390000009529.
HOGENOM	HOG000228264.
HOVERGEN	HBG000760.
InParanoid	P17694.
KO	K03935.
OMA	QSNRIIK.
OrthoDB	EOG4D7Z5H.
Family and domain databases
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR022885. NDH1_su_D/H. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20810161.

Entry information

Entry name

NDUS2_BOVIN

Accession

Primary (citable) accession number: P17694
Secondary accession number(s): Q0P5K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	October 17, 2006
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents