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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Gene

NDUFS2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.2 Publications

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi326Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi332Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi347Iron-sulfur (4Fe-4S)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

  • response to oxidative stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BRENDAi1.6.5.3. 908.
ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-49kD
Short name:
CI-49kD
NADH-ubiquinone oxidoreductase 49 kDa subunit
Gene namesi
Name:NDUFS2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

GO - Cellular componenti

  • mitochondrial respiratory chain complex I Source: UniProtKB
  • mitochondrion Source: UniProtKB
  • nucleoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33Mitochondrion1 PublicationAdd BLAST33
ChainiPRO_000011858234 – 463NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialAdd BLAST430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei62N6-acetyllysineBy similarity1
Modified residuei118Symmetric dimethylarginine1 Publication1

Post-translational modificationi

Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex.By similarity

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

PaxDbiP17694.
PRIDEiP17694.

Expressioni

Gene expression databases

BgeeiENSBTAG00000002203.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits (PubMed:10852722, PubMed:18721790). Component of the iron-sulfur (IP) fragment of the enzyme (PubMed:10852722, PubMed:18721790). Interacts with NDUFAF3 (By similarity). Interacts with NDUFAF7 (By similarity).By similarity2 Publications

Protein-protein interaction databases

IntActiP17694. 3 interactors.
STRINGi9913.ENSBTAP00000002852.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LC5electron microscopy4.35D38-463[»]
5LDWelectron microscopy4.27D35-463[»]
5LDXelectron microscopy5.60D35-463[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2870. Eukaryota.
COG0649. LUCA.
GeneTreeiENSGT00390000009529.
HOGENOMiHOG000228264.
HOVERGENiHBG000760.
InParanoidiP17694.
KOiK03935.
OMAiMADVITI.
OrthoDBiEOG091G0WWQ.
TreeFamiTF300370.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD. 1 hit.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17694-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALRALCRL RGAAAQVLRP GAGVRLPIQP SRGARQWQPD VEWAEQYGGA
60 70 80 90 100
VMYPTKETAH WKPPPWNDVD PPKDTLVSNL TLNFGPQHPA AHGVLRLVME
110 120 130 140 150
LSGEMVRKCD PHIGLLHRGT EKLIEYKTYL QALPYFDRLD YVSMMCNEQA
160 170 180 190 200
YSLAVEKLLN IQPPPRAQWI RVLFGEITRL LNHIMAVTTH ALDIGAMTPF
210 220 230 240 250
FWMFEEREKM FEFYERVSGA RMHAAYVRPG GVHQDLPLGL MDDIYEFSKN
260 270 280 290 300
FSLRIDELEE MLTNNRIWRN RTVDIGIVTA EDALNYGFSG VMLRGSGIQW
310 320 330 340 350
DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS IRIISQCLNK
360 370 380 390 400
MPPGEIKVDD AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI
410 420 430 440 450
EAPKGEFGVY LVSDGSSRPY RCKIKAPGFA HLAGLDKMSK GHMLADVVAI
460
IGTQDIVFGE VDR
Length:463
Mass (Da):52,556
Last modified:October 17, 2006 - v2
Checksum:i7EE8B22C289EA76F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162Q → R in CAA32523 (PubMed:2498081).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC119924 mRNA. Translation: AAI19925.1.
X14338 mRNA. Translation: CAA32523.1.
PIRiS04104.
RefSeqiNP_001068605.1. NM_001075137.1.
UniGeneiBt.4475.

Genome annotation databases

EnsembliENSBTAT00000002852; ENSBTAP00000002852; ENSBTAG00000002203.
GeneIDi327697.
KEGGibta:327697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC119924 mRNA. Translation: AAI19925.1.
X14338 mRNA. Translation: CAA32523.1.
PIRiS04104.
RefSeqiNP_001068605.1. NM_001075137.1.
UniGeneiBt.4475.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LC5electron microscopy4.35D38-463[»]
5LDWelectron microscopy4.27D35-463[»]
5LDXelectron microscopy5.60D35-463[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP17694. 3 interactors.
STRINGi9913.ENSBTAP00000002852.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP17694.
PRIDEiP17694.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000002852; ENSBTAP00000002852; ENSBTAG00000002203.
GeneIDi327697.
KEGGibta:327697.

Organism-specific databases

CTDi4720.

Phylogenomic databases

eggNOGiKOG2870. Eukaryota.
COG0649. LUCA.
GeneTreeiENSGT00390000009529.
HOGENOMiHOG000228264.
HOVERGENiHBG000760.
InParanoidiP17694.
KOiK03935.
OMAiMADVITI.
OrthoDBiEOG091G0WWQ.
TreeFamiTF300370.

Enzyme and pathway databases

BRENDAi1.6.5.3. 908.
ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Gene expression databases

BgeeiENSBTAG00000002203.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD. 1 hit.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS2_BOVIN
AccessioniPrimary (citable) accession number: P17694
Secondary accession number(s): Q0P5K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 17, 2006
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.