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P17694

- NDUS2_BOVIN

UniProt

P17694 - NDUS2_BOVIN

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Gene

NDUFS2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 4Fe-4S cluster.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi326 – 3261Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi332 – 3321Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)Sequence Analysis

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NAD binding Source: InterPro
    4. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
    5. quinone binding Source: InterPro

    GO - Biological processi

    1. response to oxidative stress Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Enzyme and pathway databases

    ReactomeiREACT_223064. Respiratory electron transport.

    Protein family/group databases

    TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-49kD
    Short name:
    CI-49kD
    NADH-ubiquinone oxidoreductase 49 kDa subunit
    Gene namesi
    Name:NDUFS2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 3

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: UniProtKB
    2. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333Mitochondrion1 PublicationAdd
    BLAST
    Chaini34 – 463430NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialPRO_0000118582Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei62 – 621N6-acetyllysineBy similarity
    Modified residuei118 – 1181Symmetric dimethylarginine1 Publication

    Keywords - PTMi

    Acetylation, Methylation

    Proteomic databases

    PRIDEiP17694.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits. Interacts with NDUFAF3 and NDUFAF7 By similarity. Component of the iron-sulfur (IP) fragment of the enzyme.By similarity

    Protein-protein interaction databases

    IntActiP17694. 3 interactions.
    STRINGi9913.ENSBTAP00000002852.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 49 kDa subunit family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0649.
    GeneTreeiENSGT00390000009529.
    HOGENOMiHOG000228264.
    HOVERGENiHBG000760.
    InParanoidiP17694.
    KOiK03935.
    OMAiARFTINY.
    OrthoDBiEOG7WT411.
    TreeFamiTF300370.

    Family and domain databases

    Gene3Di1.10.645.10. 1 hit.
    HAMAPiMF_01358. NDH1_NuoD.
    InterProiIPR001135. NADH_Q_OxRdtase_suD.
    IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
    IPR022885. NDH1_su_D/H.
    IPR029014. NiFe_Hase-like.
    [Graphical view]
    PfamiPF00346. Complex1_49kDa. 1 hit.
    [Graphical view]
    SUPFAMiSSF56762. SSF56762. 1 hit.
    TIGRFAMsiTIGR01962. NuoD. 1 hit.
    PROSITEiPS00535. COMPLEX1_49K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P17694-1 [UniParc]FASTAAdd to Basket

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    MAALRALCRL RGAAAQVLRP GAGVRLPIQP SRGARQWQPD VEWAEQYGGA    50
    VMYPTKETAH WKPPPWNDVD PPKDTLVSNL TLNFGPQHPA AHGVLRLVME 100
    LSGEMVRKCD PHIGLLHRGT EKLIEYKTYL QALPYFDRLD YVSMMCNEQA 150
    YSLAVEKLLN IQPPPRAQWI RVLFGEITRL LNHIMAVTTH ALDIGAMTPF 200
    FWMFEEREKM FEFYERVSGA RMHAAYVRPG GVHQDLPLGL MDDIYEFSKN 250
    FSLRIDELEE MLTNNRIWRN RTVDIGIVTA EDALNYGFSG VMLRGSGIQW 300
    DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS IRIISQCLNK 350
    MPPGEIKVDD AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI 400
    EAPKGEFGVY LVSDGSSRPY RCKIKAPGFA HLAGLDKMSK GHMLADVVAI 450
    IGTQDIVFGE VDR 463
    Length:463
    Mass (Da):52,556
    Last modified:October 17, 2006 - v2
    Checksum:i7EE8B22C289EA76F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti162 – 1621Q → R in CAA32523. (PubMed:2498081)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC119924 mRNA. Translation: AAI19925.1.
    X14338 mRNA. Translation: CAA32523.1.
    PIRiS04104.
    RefSeqiNP_001068605.1. NM_001075137.1.
    UniGeneiBt.4475.

    Genome annotation databases

    EnsembliENSBTAT00000002852; ENSBTAP00000002852; ENSBTAG00000002203.
    GeneIDi327697.
    KEGGibta:327697.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC119924 mRNA. Translation: AAI19925.1 .
    X14338 mRNA. Translation: CAA32523.1 .
    PIRi S04104.
    RefSeqi NP_001068605.1. NM_001075137.1.
    UniGenei Bt.4475.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P17694. 3 interactions.
    STRINGi 9913.ENSBTAP00000002852.

    Protein family/group databases

    TCDBi 3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Proteomic databases

    PRIDEi P17694.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000002852 ; ENSBTAP00000002852 ; ENSBTAG00000002203 .
    GeneIDi 327697.
    KEGGi bta:327697.

    Organism-specific databases

    CTDi 4720.

    Phylogenomic databases

    eggNOGi COG0649.
    GeneTreei ENSGT00390000009529.
    HOGENOMi HOG000228264.
    HOVERGENi HBG000760.
    InParanoidi P17694.
    KOi K03935.
    OMAi ARFTINY.
    OrthoDBi EOG7WT411.
    TreeFami TF300370.

    Enzyme and pathway databases

    Reactomei REACT_223064. Respiratory electron transport.

    Miscellaneous databases

    NextBioi 20810161.

    Family and domain databases

    Gene3Di 1.10.645.10. 1 hit.
    HAMAPi MF_01358. NDH1_NuoD.
    InterProi IPR001135. NADH_Q_OxRdtase_suD.
    IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
    IPR022885. NDH1_su_D/H.
    IPR029014. NiFe_Hase-like.
    [Graphical view ]
    Pfami PF00346. Complex1_49kDa. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56762. SSF56762. 1 hit.
    TIGRFAMsi TIGR01962. NuoD. 1 hit.
    PROSITEi PS00535. COMPLEX1_49K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal muscle.
    2. "A homologue of the nuclear coded 49 kd subunit of bovine mitochondrial NADH-ubiquinone reductase is coded in chloroplast DNA."
      Fearnley I.M., Runswick M.J., Walker J.E.
      EMBO J. 8:665-672(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-463, PROTEIN SEQUENCE OF 34-55.
      Tissue: Heart.
    3. "Post-translational modifications near the quinone binding site of mammalian complex I."
      Carroll J., Ding S., Fearnley I.M., Walker J.E.
      J. Biol. Chem. 288:24799-24808(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT ARG-118.

    Entry informationi

    Entry nameiNDUS2_BOVIN
    AccessioniPrimary (citable) accession number: P17694
    Secondary accession number(s): Q0P5K5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3