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P17694

- NDUS2_BOVIN

UniProt

P17694 - NDUS2_BOVIN

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Protein
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
Gene
NDUFS2
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.UniRule annotation

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).UniRule annotation
NADH + acceptor = NAD+ + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi326 – 3261Iron-sulfur (4Fe-4S) Reviewed prediction
Metal bindingi332 – 3321Iron-sulfur (4Fe-4S) Reviewed prediction
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S) Reviewed prediction

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. NAD binding Source: InterPro
  3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW
  5. quinone binding Source: InterPro

GO - Biological processi

  1. response to oxidative stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiREACT_223064. Respiratory electron transport.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-49kD
Short name:
CI-49kD
NADH-ubiquinone oxidoreductase 49 kDa subunit
Gene namesi
Name:NDUFS2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 3

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial respiratory chain complex I Source: UniProtKB
  2. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333Mitochondrion1 Publication
Add
BLAST
Chaini34 – 463430NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialUniRule annotation
PRO_0000118582Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621N6-acetyllysine By similarity
Modified residuei118 – 1181Symmetric dimethylarginine1 Publication

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

PRIDEiP17694.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. Interacts with NDUFAF3 and NDUFAF7 By similarity. Component of the iron-sulfur (IP) fragment of the enzyme.

Protein-protein interaction databases

IntActiP17694. 3 interactions.
STRINGi9913.ENSBTAP00000002852.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0649.
GeneTreeiENSGT00390000009529.
HOGENOMiHOG000228264.
HOVERGENiHBG000760.
InParanoidiP17694.
KOiK03935.
OMAiARFTINY.
OrthoDBiEOG7WT411.
TreeFamiTF300370.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17694-1 [UniParc]FASTAAdd to Basket

« Hide

MAALRALCRL RGAAAQVLRP GAGVRLPIQP SRGARQWQPD VEWAEQYGGA    50
VMYPTKETAH WKPPPWNDVD PPKDTLVSNL TLNFGPQHPA AHGVLRLVME 100
LSGEMVRKCD PHIGLLHRGT EKLIEYKTYL QALPYFDRLD YVSMMCNEQA 150
YSLAVEKLLN IQPPPRAQWI RVLFGEITRL LNHIMAVTTH ALDIGAMTPF 200
FWMFEEREKM FEFYERVSGA RMHAAYVRPG GVHQDLPLGL MDDIYEFSKN 250
FSLRIDELEE MLTNNRIWRN RTVDIGIVTA EDALNYGFSG VMLRGSGIQW 300
DLRKTQPYDV YDQVEFDVPI GSRGDCYDRY LCRVEEMRQS IRIISQCLNK 350
MPPGEIKVDD AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI 400
EAPKGEFGVY LVSDGSSRPY RCKIKAPGFA HLAGLDKMSK GHMLADVVAI 450
IGTQDIVFGE VDR 463
Length:463
Mass (Da):52,556
Last modified:October 17, 2006 - v2
Checksum:i7EE8B22C289EA76F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621Q → R in CAA32523. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC119924 mRNA. Translation: AAI19925.1.
X14338 mRNA. Translation: CAA32523.1.
PIRiS04104.
RefSeqiNP_001068605.1. NM_001075137.1.
UniGeneiBt.4475.

Genome annotation databases

EnsembliENSBTAT00000002852; ENSBTAP00000002852; ENSBTAG00000002203.
GeneIDi327697.
KEGGibta:327697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC119924 mRNA. Translation: AAI19925.1 .
X14338 mRNA. Translation: CAA32523.1 .
PIRi S04104.
RefSeqi NP_001068605.1. NM_001075137.1.
UniGenei Bt.4475.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P17694. 3 interactions.
STRINGi 9913.ENSBTAP00000002852.

Protein family/group databases

TCDBi 3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PRIDEi P17694.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000002852 ; ENSBTAP00000002852 ; ENSBTAG00000002203 .
GeneIDi 327697.
KEGGi bta:327697.

Organism-specific databases

CTDi 4720.

Phylogenomic databases

eggNOGi COG0649.
GeneTreei ENSGT00390000009529.
HOGENOMi HOG000228264.
HOVERGENi HBG000760.
InParanoidi P17694.
KOi K03935.
OMAi ARFTINY.
OrthoDBi EOG7WT411.
TreeFami TF300370.

Enzyme and pathway databases

Reactomei REACT_223064. Respiratory electron transport.

Miscellaneous databases

NextBioi 20810161.

Family and domain databases

Gene3Di 1.10.645.10. 1 hit.
HAMAPi MF_01358. NDH1_NuoD.
InterProi IPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view ]
Pfami PF00346. Complex1_49kDa. 1 hit.
[Graphical view ]
SUPFAMi SSF56762. SSF56762. 1 hit.
TIGRFAMsi TIGR01962. NuoD. 1 hit.
PROSITEi PS00535. COMPLEX1_49K. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal muscle.
  2. "A homologue of the nuclear coded 49 kd subunit of bovine mitochondrial NADH-ubiquinone reductase is coded in chloroplast DNA."
    Fearnley I.M., Runswick M.J., Walker J.E.
    EMBO J. 8:665-672(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-463, PROTEIN SEQUENCE OF 34-55.
    Tissue: Heart.
  3. "Post-translational modifications near the quinone binding site of mammalian complex I."
    Carroll J., Ding S., Fearnley I.M., Walker J.E.
    J. Biol. Chem. 288:24799-24808(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT ARG-118.

Entry informationi

Entry nameiNDUS2_BOVIN
AccessioniPrimary (citable) accession number: P17694
Secondary accession number(s): Q0P5K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 17, 2006
Last modified: September 3, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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