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P17693 (HLAG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HLA class I histocompatibility antigen, alpha chain G
Alternative name(s):
HLA G antigen
MHC class I antigen G
Gene names
Name:HLA-G
Synonyms:HLA-6.0, HLAG
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells.

Subunit structure

Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Homodimer; disulfide-linked. Binds to LILRB1 and LILRB2. Ref.6 Ref.8

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed in trophoblasts.

Sequence similarities

Belongs to the MHC class I family.

Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processImmunity
   Cellular componentMembrane
MHC I
   DomainSignal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent

Traceable author statement. Source: Reactome

antigen processing and presentation of peptide antigen via MHC class I

Traceable author statement. Source: Reactome

cellular defense response

Traceable author statement PubMed 9539768. Source: ProtInc

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

immune response-inhibiting cell surface receptor signaling pathway

Inferred from direct assay PubMed 20702625. Source: BHF-UCL

interferon-gamma-mediated signaling pathway

Traceable author statement. Source: Reactome

negative regulation of T cell proliferation

Inferred from direct assay PubMed 20702625. Source: BHF-UCL

negative regulation of dendritic cell differentiation

Inferred from direct assay PubMed 20702625. Source: BHF-UCL

positive regulation of T cell mediated cytotoxicity

Inferred from electronic annotation. Source: InterPro

positive regulation of T cell tolerance induction

Inferred from mutant phenotype PubMed 20448110. Source: BHF-UCL

positive regulation of interleukin-12 production

Inferred from direct assay PubMed 20702625. Source: BHF-UCL

positive regulation of regulatory T cell differentiation

Inferred from mutant phenotype PubMed 20448110. Source: BHF-UCL

regulation of immune response

Traceable author statement. Source: Reactome

type I interferon signaling pathway

Traceable author statement. Source: Reactome

   Cellular_componentER to Golgi transport vesicle membrane

Traceable author statement. Source: Reactome

Golgi membrane

Traceable author statement. Source: Reactome

MHC class I protein complex

Inferred from electronic annotation. Source: UniProtKB-KW

early endosome membrane

Traceable author statement. Source: Reactome

integral component of lumenal side of endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

phagocytic vesicle membrane

Traceable author statement. Source: Reactome

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionpeptide antigen binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein homodimerization activity

Non-traceable author statement PubMed 18550825. Source: BHF-UCL

receptor binding

Inferred from physical interaction PubMed 20702625. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424
Chain25 – 338314HLA class I histocompatibility antigen, alpha chain G
PRO_0000018886

Regions

Topological domain25 – 308284Extracellular Potential
Transmembrane309 – 33224Helical; Potential
Topological domain333 – 3386Cytoplasmic Potential
Domain209 – 29991Ig-like C1-type
Region25 – 11490Alpha-1
Region115 – 20692Alpha-2
Region207 – 29892Alpha-3
Region299 – 30810Connecting peptide

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) By similarity
Disulfide bond66Interchain Ref.6 Ref.8
Disulfide bond125 ↔ 188 Ref.8
Disulfide bond227 ↔ 283 Ref.8

Experimental info

Mutagenesis661C → S: Abolishes homodimerization. Ref.6

Secondary structure

..................................................... 338
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P17693 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 0678E263BE8962FF

FASTA33838,224
        10         20         30         40         50         60 
MVVMAPRTLF LLLSGALTLT ETWAGSHSMR YFSAAVSRPG RGEPRFIAMG YVDDTQFVRF 

        70         80         90        100        110        120 
DSDSACPRME PRAPWVEQEG PEYWEEETRN TKAHAQTDRM NLQTLRGYYN QSEASSHTLQ 

       130        140        150        160        170        180 
WMIGCDLGSD GRLLRGYEQY AYDGKDYLAL NEDLRSWTAA DTAAQISKRK CEAANVAEQR 

       190        200        210        220        230        240 
RAYLEGTCVE WLHRYLENGK EMLQRADPPK THVTHHPVFD YEATLRCWAL GFYPAEIILT 

       250        260        270        280        290        300 
WQRDGEDQTQ DVELVETRPA GDGTFQKWAA VVVPSGEEQR YTCHVQHEGL PEPLMLRWKQ 

       310        320        330 
SSLPTIPIMG IVAGLVVLAA VVTGAAVAAV LWRKKSSD 

« Hide

References

« Hide 'large scale' references
[1]"The mRNA of a human class I gene HLA G/HLA 6.0 exhibits a restricted pattern of expression."
Shukla H., Swaroop A., Srivastava R., Weissman S.M.
Nucleic Acids Res. 18:2189-2189(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fetal eye.
[2]"A human major histocompatibility complex class I gene that encodes a protein with a shortened cytoplasmic segment."
Geraghty D.E., Koller B.H., Orr H.T.
Proc. Natl. Acad. Sci. U.S.A. 84:9145-9149(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]Ishitani A., Geraghty D.E.
Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[4]"A 356-Kb sequence of the subtelomeric part of the MHC class I region."
Hampe A., Coriton O., Andrieux N., Carn G., Lepourcelet M., Mottier S., Dreano S., Gatius M.T., Hitte C., Soriano N., Galibert F.
DNA Seq. 10:263-299(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region."
Shiina S., Tamiya G., Oka A., Inoko H.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"Disulfide bond-mediated dimerization of HLA-G on the cell surface."
Boyson J.E., Erskine R., Whitman M.C., Chiu M., Lau J.M., Koopman L.A., Valter M.M., Angelisova P., Horejsi V., Strominger J.L.
Proc. Natl. Acad. Sci. U.S.A. 99:16180-16185(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, MUTAGENESIS OF CYS-66, DISULFIDE BOND AT CYS-66.
[7]"Crystal structure of HLA-G: a nonclassical MHC class I molecule expressed at the fetal-maternal interface."
Clements C.S., Kjer-Nielsen L., Kostenko L., Hoare H.L., Dunstone M.A., Moses E., Freed K., Brooks A.G., Rossjohn J., McCluskey J.
Proc. Natl. Acad. Sci. U.S.A. 102:3360-3365(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 26-300.
[8]"Efficient leukocyte Ig-like receptor signaling and crystal structure of disulfide-linked HLA-G dimer."
Shiroishi M., Kuroki K., Ose T., Rasubala L., Shiratori I., Arase H., Tsumoto K., Kumagai I., Kohda D., Maenaka K.
J. Biol. Chem. 281:10439-10447(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 25-300, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17273 mRNA. Translation: CAA35174.1.
J03027 Genomic DNA. Translation: AAA98745.1.
M90683 mRNA. Translation: AAA52673.1.
AF055066 Genomic DNA. Translation: AAC24826.1.
BA000025 Genomic DNA. Translation: BAB63336.1.
PIRA39953.
RefSeqNP_002118.1. NM_002127.5.
UniGeneHs.512152.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YDPX-ray1.90A26-300[»]
2D31X-ray3.20A/D25-300[»]
2DYPX-ray2.50A25-300[»]
3BZEX-ray2.50P/Q/R/S3-11[»]
3CDGX-ray3.40P/Q3-11[»]
3CIIX-ray4.41C/F3-11[»]
3KYNX-ray2.40A26-299[»]
3KYOX-ray1.70A/C26-298[»]
ProteinModelPortalP17693.
SMRP17693. Positions 26-300.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109380. 10 interactions.
DIPDIP-46121N.
IntActP17693. 1 interaction.
STRING9606.ENSP00000387624.

PTM databases

PhosphoSiteP17693.

Polymorphism databases

DMDM122132.

Proteomic databases

PaxDbP17693.
PRIDEP17693.

Protocols and materials databases

DNASU3135.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000360323; ENSP00000353472; ENSG00000204632.
ENST00000383621; ENSP00000373116; ENSG00000206506.
ENST00000423011; ENSP00000389522; ENSG00000233095.
ENST00000423373; ENSP00000405238; ENSG00000235346.
ENST00000428701; ENSP00000412927; ENSG00000204632.
ENST00000428952; ENSP00000388176; ENSG00000235680.
ENST00000444098; ENSP00000398200; ENSG00000230413.
ENST00000449127; ENSP00000408773; ENSG00000237216.
ENST00000546545; ENSP00000447762; ENSG00000230413.
ENST00000546634; ENSP00000447780; ENSG00000235346.
ENST00000547241; ENSP00000448085; ENSG00000233095.
ENST00000547931; ENSP00000448363; ENSG00000237216.
ENST00000550897; ENSP00000449903; ENSG00000235680.
ENST00000553052; ENSP00000449291; ENSG00000206506.
GeneID3135.
KEGGhsa:3135.
UCSCuc003nnw.2. human.

Organism-specific databases

CTD3135.
GeneCardsGC06P029794.
GC06Pj29788.
GC06Pk29791.
GC06Pl29791.
GC06Pm29791.
GC06Pn29791.
GC06Po29791.
H-InvDBHIX0166674.
HIX0167370.
HGNCHGNC:4964. HLA-G.
HPACAB016158.
MIM142871. gene.
neXtProtNX_P17693.
PharmGKBPA35083.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42056.
HOVERGENHBG016709.
InParanoidP17693.
KOK06751.
PhylomeDBP17693.
TreeFamTF336617.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressP17693.
BgeeP17693.
CleanExHS_HLA-G.
GenevestigatorP17693.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]
PfamPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
[Graphical view]
PRINTSPR01638. MHCCLASSI.
SMARTSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMSSF54452. SSF54452. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHLA-G. human.
EvolutionaryTraceP17693.
GeneWikiHLA-G.
GenomeRNAi3135.
NextBio12439.
PROP17693.
SOURCESearch...

Entry information

Entry nameHLAG_HUMAN
AccessionPrimary (citable) accession number: P17693
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 16, 2014
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM