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Reviewed, UniProtKB/Swiss-Prot P17692 (CDGT_BACS8)

Last modified June 16, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cyclomaltodextrin glucanotransferase
    EC=2.4.1.19
Alternative name(s):
    Cyclodextrin-glycosyltransferase
      Short name=CGTase
    Raw-starch-digesting amylase
Gene names
Name: cgt
OrganismBacillus sp. (strain B1018)
Taxonomic identifier1417 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length713 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This endo-type adsorbable amylase is capable to digest raw starch.

Catalytic activity

Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.

Cofactor

Binds 2 calcium ions per subunit By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Contains 1 CBM20 (carbohydrate binding type-20) domain.

Contains 1 IPT/TIG domain.

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Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionGlycosyltransferase
Transferase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

cyclomaltodextrin glucanotransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.1
Chain28 – 713686Cyclomaltodextrin glucanotransferase
PRO_0000001433

Regions

Domain526 – 60782IPT/TIG
Domain608 – 713106CBM20

Sites

Active site2561 By similarity
Active site2841 By similarity
Active site3551 By similarity
Metal binding541Calcium 1 By similarity
Metal binding561Calcium 1; via carbonyl oxygen By similarity
Metal binding591Calcium 1 By similarity
Metal binding601Calcium 1 By similarity
Metal binding801Calcium 1 By similarity
Metal binding1661Calcium 2 By similarity
Metal binding2171Calcium 2; via carbonyl oxygen By similarity
Metal binding2261Calcium 2 By similarity
Metal binding2601Calcium 2; via carbonyl oxygen By similarity

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Sequences

Sequence LengthMass (Da)Tools
P17692-1 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 85FB616DA687B888

FASTA71377,421
        10         20         30         40         50         60 
MKKFLKMTAA FSLGLSLAFG LFSPAQAAPD TSVSNKQNFS TDVIYQIFTD RFSDGNPANN 

        70         80         90        100        110        120 
PTGAAFDGTC TNLRLYCGGD WQGIINKIND GYLTGMGVTA IWISQPVENI YSIINYSGVN 

       130        140        150        160        170        180 
NTAYHGYWAR DFKKTNPAYG TIADFQNLIA AAHAKNIKVI IDFAPNHTSP ASSDQPSFAE 

       190        200        210        220        230        240 
NGRLYDNGTL LGGYTNDTQN LFHHNGGTDF STTENGIYKN LYDLADLNHN NSTSDVYLKD 

       250        260        270        280        290        300 
AIKMWLDLGI DGIRMDAVKH MPFGWQKSFM AAVNNYKPVF TFGEWFLGVN EVGPENHKFA 

       310        320        330        340        350        360 
NESGMSLLDF RFAQKVRQVF RDNTDNMYGL KAMLEGSAAD YAQVDDQVTF IDNHDMERFH 

       370        380        390        400        410        420 
ASNANRRKLE QALAFTLILA RVPAIYYGTE QYMSGGTDPD NRARIPSFST STTAYQVIQK 

       430        440        450        460        470        480 
LAPLRKSNPA IAYGSTQERW INNDVLIYER KFGSNVAVVA VNRNLNAPAS ISGLVTSLPQ 

       490        500        510        520        530        540 
GSYNDVLGGL LNGNTLTVGS GGAASNFTLA AGGTAVWQYT AATATPTIGH VGPMMAKPGV 

       550        560        570        580        590        600 
TITIDGRGFG SSKGTVYFGT TAVSGANITS WEDTQIKVKI PAVAGGIYNI KVANAAGTAS 

       610        620        630        640        650        660 
NVYDNFEVLS GDQVSVRFVV NNATTALGQN LYLTGNVSEL GNWDPAKAIG PMYNQVVYQY 

       670        680        690        700        710 
PNWYYDVSVP AGKTIEFKFL KKQGSTVTWE GGSNHTFTAP SSGTATINVN WQP

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References

[1]"Nucleotide sequence of the raw-starch-digesting amylase gene from Bacillus sp. B1018 and its strong homology to the cyclodextrin glucanotransferase genes."
Itkor P., Tsukagoshi N., Udaka S.
Biochem. Biophys. Res. Commun. 166:630-636(1990) [PubMed: 1689153] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-47.

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Cross-references

Sequence databases

M33302 Genomic DNA. Translation: AAA22239.1.
D90112 Genomic DNA. Translation: BAA14140.1.
PIRS09196.

3D structure databases

HSSPHSSP built from PDB template 1CXL based on UniProtKB P43379.
SMRP17692. Positions 28-713.
ModBaseSearch...

Protein family/group databases

CAZyCBM20. Carbohydrate-Binding Module Family 20.
GH13. Glycoside Hydrolase Family 13.

Enzyme and pathway databases

BRENDA2.4.1.19. 1000.

Family and domain databases

InterProIPR006048. A-amylase_b_C.
IPR006046. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat.
IPR006589. Glyco_hydro_13_sub_cat.
IPR002044. Glyco_hydro_carb-bd.
IPR013781. Glyco_hydro_sg_catalytic.
IPR013783. Ig-like_fold.
IPR002909. IPT_TIG_rcpt.
[Graphical view]
Gene3DG3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF00686. CBM_20. 1 hit.
PF01833. TIG. 1 hit.
[Graphical view]
PRINTSPR00110. ALPHAAMYLASE.
ProDomPD001568. Glyco_hydro_CBD. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
PROSITEPS51166. CBM20. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCDGT_BACS8
AccessionPrimary (citable) accession number: P17692
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents