P17691 (NEUM_CARAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuromodulin

Alternative name(s):
Axonal membrane protein GAP-43
Growth-associated protein 43

Gene names

Name:

gap43

Organism

Carassius auratus (Goldfish)

Taxonomic identifier

7957 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Carassius

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction By similarity.
Subunit structure	Binds calmodulin with a greater affinity in the absence of Ca2+ than in its presence By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection › growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction › synapse. Cell projection › filopodium membrane; Peripheral membrane protein By similarity. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.
Post-translational modification	Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction By similarity. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
Sequence similarities	Belongs to the neuromodulin family. Contains 1 IQ domain.

Ontologies

Keywords
Biological process	Differentiation Growth regulation Neurogenesis
Cellular component	Cell junction Cell membrane Cell projection Membrane Synapse
Ligand	Calmodulin-binding
Molecular function	Developmental protein
PTM	Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW filopodium membrane Inferred from electronic annotation. Source: UniProtKB-SubCell growth cone membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Neuromodulin		PRO_0000159602
Regions
	Domain	32 – 61	30	IQ
	Region	1 – 4	4	Important for membrane binding By similarity
	Compositional bias	155 – 170	16	Poly-Glu
Amino acid modifications
	Modified residue	42	1	Phosphoserine; by PKC By similarity
	Lipidation	3	1	S-palmitoyl cysteine By similarity
	Lipidation	4	1	S-palmitoyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17691 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 31078ACB7B1682EC	FASTA	213	23,569
	10 20 30 40 50 60 MLCCIRRTKP VEKNEEADQE IKQDGTKPEE NAHKAATKIQ ASFRGHITRK KMKDEDKDGE 70 80 90 100 110 120 NDTAPDESAE TEEKEERVSP SEEKPVEVST ETAEESKPAE QPNSPAAEAP PTAATDSAPS 130 140 150 160 170 180 DTPTKEEAQE QLQDAEEPKE TENTAAADDI TTQKEEEKEE EEEEEEEEEE AKRADVPDDT 190 200 210 PAATESQETD QTDKKEALDD SKPAEEAGKD QNV

References

[1]	"Selective conservation of GAP-43 structure in vertebrate evolution." Labate M.E., Skene J.H.P. Neuron 3:299-310(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M26250 mRNA. Translation: AAA03010.1.
PIR	JQ0075.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG006468.
Family and domain databases
InterPro	IPR000048. IQ_motif_EF-hand-BS. IPR001422. Neuromodulin. IPR018947. Neuromodulin_gap-junction_N. IPR018243. Neuromodulin_palmitoyl/P_site. [Graphical view]
Pfam	PF00612. IQ. 1 hit. PF10580. Neuromodulin_N. 1 hit. [Graphical view]
PRINTS	PR00215. NEUROMODULIN.
SMART	SM00015. IQ. 1 hit. [Graphical view]
PROSITE	PS50096. IQ. 1 hit. PS00412. NEUROMODULIN_1. 1 hit. PS00413. NEUROMODULIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NEUM_CARAU

Accession

Primary (citable) accession number: P17691

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program