P17688 (AROA_BRANA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic EC=2.5.1.19 Alternative name(s): 5-enolpyruvylshikimate-3-phosphate synthase Short name=EPSP synthase
Organism	Brassica napus (Rape)
Taxonomic identifier	3708 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.
Subcellular location	Plastid › chloroplast.
Miscellaneous	This enzyme is the target of the potent, broad-spectrum herbicide, glyphosate [n-(phosphonomethyl)glycine]. Overproduction of EPSP leads to glyphosate tolerance.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 72	72	Chloroplast Potential
	Chain	73 – 516	444	3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic		PRO_0000002288

Sequences

	Sequence		Length	Mass (Da)
	P17688 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 86804B4DE4D0DF57	FASTA	516	55,030
	10 20 30 40 50 60 MAQSSRICHG VQNPCVIISN LSKSNQNKSP FSVSLKTHQP RASSWGLKKS GTMLNGSVIR 70 80 90 100 110 120 PVKVTASVST SEKASEIVLQ PIREISGLIK LPGSKSLSNR ILLLAALSEG TTVVDNLLNS 130 140 150 160 170 180 DDINYMLDAL KKLGLNVERD SVNNRAVVEG CGGIFPASLD SKSDIELYLG NAGTAMRPLT 190 200 210 220 230 240 AAVTAAGGNA SYVLDGVPRM RERPIGDLVV GLKQLGADVE CTLGTNCPPV RVNANGGLPG 250 260 270 280 290 300 GKVKLSGSIS SQYLTALLMA APLALGDVEI EIIDKLISVP YVEMTLKLME RFGVSAEHSD 310 320 330 340 350 360 SWDRFFVKGG QKYKSPGNAY VEGDASSASY FLAGAAITGE TVTVEGCGTT SLQGDVKFAE 370 380 390 400 410 420 VLEKMGCKVS WTENSVTVTG PSRDAFGMRH LRAVDVNMNK MPDVAMTLAV VALFADGPTT 430 440 450 460 470 480 IRDVASWRVK ETERMIAICT ELRKLGATVE EGSDYCVITP PAKVKPAEID TYDDHRMAMA 490 500 510 FSLAACADVP VTIKDPGCTR KTFPDYFQVL ESITKH

References

[1]	"A Brassica napus gene encoding 5-enolpyruvylshikimate-3-phosphate synthase." Gasser C.S., Klee H.J. Nucleic Acids Res. 18:2821-2821(1990) [PubMed: 2339069] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Westar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X51475 Genomic DNA. Translation: CAA35839.1.
PIR	S12744.
3D structure databases
ProteinModelPortal	P17688.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BRANA

Accession

Primary (citable) accession number: P17688

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	September 21, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program