Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P17654 (AMY1_ORYSJ)

Last modified June 16, 2009. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Alpha-amylase
    EC=3.2.1.1
Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
    Alpha-amylase isozyme 1B
Gene names
Name: AMY1.1
Synonyms: AMY1A
Ordered Locus Names: Os02g0765600, LOC_Os02g52710
ORF Names: OJ1004_A11.13, P0539D10.32
OrganismOryza sativa subsp. japonica (Rice)
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Hide | Top

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Function

Important for breakdown of endosperm starch during germination.

Catalytic activity

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.

Cofactor

Binds 3 calcium ions per subunit By similarity.

Subunit structure

Monomer.

Tissue specificity

More abundant in germinating seeds, than in callus, young roots and leaves.

Developmental stage

Expressed at a high level during germination in the aleurones cells under the control of the plant hormone gibberellic acid and in the developing grains at a low level.

Post-translational modification

Only cereal amylase known to be glycosylated.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Hide | Top

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processstarch catabolic process Ref.1 Ref.2

Inferred from expression pattern. Source: Gramene

sucrose catabolic process Ref.1 Ref.2

Inferred from expression pattern. Source: Gramene

   Molecular functionalpha-amylase activity

Inferred from electronic annotation. Source: EC

calcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Probable
Chain32 – 434403Alpha-amylase
PRO_0000001408

Sites

Active site2091Nucleophile By similarity
Active site2341Proton donor By similarity
Active site3201 By similarity
Metal binding1221Calcium 1 By similarity
Metal binding1391Calcium 2 By similarity
Metal binding1421Calcium 2 By similarity
Metal binding1441Calcium 2 By similarity
Metal binding1481Calcium 2 By similarity
Metal binding1581Calcium 3 By similarity
Metal binding1681Calcium 3 By similarity
Metal binding1711Calcium 3; via carbonyl oxygen By similarity
Metal binding1721Calcium 1 By similarity
Metal binding1731Calcium 1; via carbonyl oxygen By similarity
Metal binding1761Calcium 3; via carbonyl oxygen By similarity
Metal binding1781Calcium 1 By similarity
Metal binding1781Calcium 3 By similarity

Amino acid modifications

Glycosylation2711N-linked (GlcNAc...) Probable

Experimental info

Sequence conflict2721A → G Ref.1
Sequence conflict2721A → G Ref.2

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P17654-1 [UniParc].

Last modified October 25, 2005. Version 2.
Checksum: 7B23B299A0AD3E37

FASTA43448,457
        10         20         30         40         50         60 
MQVLNTMVNK HFLSLSVLIV LLGLSSNLTA GQVLFQGFNW ESWKENGGWY NFLMGKVDDI 

        70         80         90        100        110        120 
AAAGITHVWL PPPSHSVGEQ GYMPGRLYDL DASKYGNEAQ LKSLIEAFHG KGVQVIADIV 

       130        140        150        160        170        180 
INHRTAEHKD GRGIYCLFEG GTPDSRLDWG PHMICRDDPY GDGTGNPDTG ADFAAAPDID 

       190        200        210        220        230        240 
HLNKRVQREL IGWLDWLKMD IGFDAWRLDF AKGYSADMAK IYIDATEPSF AVAEIWTSMA 

       250        260        270        280        290        300 
NGGDGKPNYD QNAHRQELVN WVDRVGGANS NATAFDFTTK GILNVAVEGE LWRLRGEDGK 

       310        320        330        340        350        360 
APGMIGWWPA KATTFVDNHD TGSTQHLWPF PSDKVMQGYA YILTHPGNPC IFYDHFFDWG 

       370        380        390        400        410        420 
LKEEIERLVS IRNRQGIHPA SELRIMEADS DLYLAEIDGK VITKIGPRYD VEHLIPEGFQ 

       430 
VVAHGDGYAI WEKI

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"The alpha-amylase genes in Oryza sativa: characterization of cDNA clones and mRNA expression during seed germination."
O'Neill S.D., Kumagai M.H., Majumdar A., Huang N., Sutliff T.D., Rodriguez R.L.
Mol. Gen. Genet. 221:235-244(1990) [PubMed: 2370848] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. M202.
[2]"Classification and characterization of the rice alpha-amylase multigene family."
Huang N., Sutliff T.D., Litts J.C., Rodriguez R.L.
Plant Mol. Biol. 14:655-668(1990) [PubMed: 2102847] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. M202.
[3]"Oryza sativa nipponbare chromosome 2 genomic DNA sequence."
The international rice genome sequencing project (IRGSP) consortium
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.

Hide | Top

Cross-references

Sequence databases

M24286 mRNA. Translation: AAA33885.1.
X16509 Genomic DNA. Translation: CAA34516.1. Different initiation.
AP004817 Genomic DNA. Translation: BAD17125.1.
AP005287 Genomic DNA. Translation: BAD17313.1.
PIRS10013.
S12775.
RefSeqNP_001048220.1.
UniGeneOs.49249

3D structure databases

HSSPHSSP built from PDB template 1AVA based on UniProtKB P04063.
SMRP17654. Positions 32-433.
ModBaseSearch...

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Genome annotation databases

GeneID4330832.
KEGGosa:4330832.

Organism-specific databases

GrameneP17654.

Family and domain databases

InterProIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat.
IPR006589. Glyco_hydro_13_sub_cat.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSPR00110. ALPHAAMYLASE.
SMARTSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameAMY1_ORYSJ
AccessionPrimary (citable) accession number: P17654
Secondary accession number(s): Q6Z317
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 25, 2005
Last modified: June 16, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents