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Protein

4-aminobutyrate aminotransferase

Gene

UGA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the degradation of gamma-aminobutyric acid (GABA), which is important for utilization of GABA as nitrogen source and for oxidative stress tolerance. Deaminates GABA to succinate semialdehyde, which in turn is converted to succinate by the succinate-semialdehyde dehydrogenase UGA2. Cannot transaminate beta-alanine (BAL).3 Publications

Catalytic activityi

4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=3.2 mM for 4-aminobutanoate1 Publication
  2. KM=0.22 mM for 2-oxoglutarate1 Publication
  1. Vmax=12.8 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192SubstrateBy similarity1
Binding sitei351Pyridoxal phosphate; shared with dimeric partnerBy similarity1

GO - Molecular functioni

  • 4-aminobutyrate transaminase activity Source: SGD
  • identical protein binding Source: GO_Central
  • pyridoxal phosphate binding Source: GO_Central

GO - Biological processi

  • gamma-aminobutyric acid catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YGR019W-MONOMER.
YEAST:YGR019W-MONOMER.
BRENDAi2.6.1.19. 984.
ReactomeiR-SCE-916853. Degradation of GABA.

Names & Taxonomyi

Protein namesi
Recommended name:
4-aminobutyrate aminotransferase (EC:2.6.1.19)
Alternative name(s):
GABA aminotransferase
Short name:
GABA-AT
Gamma-amino-N-butyrate transaminase
Short name:
GABA transaminase
Gene namesi
Name:UGA1
Ordered Locus Names:YGR019W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR019W.
SGDiS000003251. UGA1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203821 – 4714-aminobutyrate aminotransferaseAdd BLAST471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei326N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

MaxQBiP17649.
PRIDEiP17649.

Expressioni

Inductioni

Subject to nitrogen catabolite repression. Expression is low in the presence of the preferred nitrogen sources, and up-regulated by GABA.1 Publication

Interactioni

Subunit structurei

Homodimer and homotetramer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33261. 39 interactors.
IntActiP17649. 2 interactors.
MINTiMINT-4486784.

Structurei

3D structure databases

ProteinModelPortaliP17649.
SMRiP17649.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 136Pyridoxal phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074885.
HOGENOMiHOG000020208.
InParanoidiP17649.
KOiK13524.
OMAiPIHKLDF.
OrthoDBiEOG092C208V.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR004631. 4NH2But_aminotransferase_euk.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 2 hits.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00699. GABAtrns_euk. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSICEQYYPE EPTKPTVKTE SIPGPESQKQ LKELGEVFDT RPAYFLADYE
60 70 80 90 100
KSLGNYITDV DGNTYLDLYA QISSIALGYN NPALIKAAQS PEMIRALVDR
110 120 130 140 150
PALGNFPSKD LDKILKQILK SAPKGQDHVW SGLSGADANE LAFKAAFIYY
160 170 180 190 200
RAKQRGYDAD FSEKENLSVM DNDAPGAPHL AVLSFKRAFH GRLFASGSTT
210 220 230 240 250
CSKPIHKLDF PAFHWPHAEY PSYQYPLDEN SDANRKEDDH CLAIVEELIK
260 270 280 290 300
TWSIPVAALI IEPIQSEGGD NHASKYFLQK LRDITLKYNV VYIIDEVQTG
310 320 330 340 350
VGATGKLWCH EYADIQPPVD LVTFSKKFQS AGYFFHDPKF IPNKPYRQFN
360 370 380 390 400
TWCGEPARMI IAGAIGQEIS DKKLTEQCSR VGDYLFKKLE GLQKKYPENF
410 420 430 440 450
QNLRGKGRGT FIAWDLPTGE KRDLLLKKLK LNGCNVGGCA VHAVRLRPSL
460 470
TFEEKHADIF IEALAKSVNE L
Length:471
Mass (Da):52,946
Last modified:October 1, 1996 - v2
Checksum:i33D446778A891F63
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti240H → R in CAA36833 (PubMed:2190186).Curated1
Sequence conflicti240H → R in ABF58895 (PubMed:17355287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52600 Genomic DNA. Translation: CAA36833.1.
DQ512723 Genomic DNA. Translation: ABF58895.1.
Z72804 Genomic DNA. Translation: CAA97002.1.
AY692904 Genomic DNA. Translation: AAT92923.1.
BK006941 Genomic DNA. Translation: DAA08115.1.
PIRiS64310.
RefSeqiNP_011533.3. NM_001181148.3.

Genome annotation databases

EnsemblFungiiYGR019W; YGR019W; YGR019W.
GeneIDi852902.
KEGGisce:YGR019W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52600 Genomic DNA. Translation: CAA36833.1.
DQ512723 Genomic DNA. Translation: ABF58895.1.
Z72804 Genomic DNA. Translation: CAA97002.1.
AY692904 Genomic DNA. Translation: AAT92923.1.
BK006941 Genomic DNA. Translation: DAA08115.1.
PIRiS64310.
RefSeqiNP_011533.3. NM_001181148.3.

3D structure databases

ProteinModelPortaliP17649.
SMRiP17649.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33261. 39 interactors.
IntActiP17649. 2 interactors.
MINTiMINT-4486784.

Proteomic databases

MaxQBiP17649.
PRIDEiP17649.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR019W; YGR019W; YGR019W.
GeneIDi852902.
KEGGisce:YGR019W.

Organism-specific databases

EuPathDBiFungiDB:YGR019W.
SGDiS000003251. UGA1.

Phylogenomic databases

GeneTreeiENSGT00550000074885.
HOGENOMiHOG000020208.
InParanoidiP17649.
KOiK13524.
OMAiPIHKLDF.
OrthoDBiEOG092C208V.

Enzyme and pathway databases

BioCyciMetaCyc:YGR019W-MONOMER.
YEAST:YGR019W-MONOMER.
BRENDAi2.6.1.19. 984.
ReactomeiR-SCE-916853. Degradation of GABA.

Miscellaneous databases

PROiP17649.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR004631. 4NH2But_aminotransferase_euk.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 2 hits.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00699. GABAtrns_euk. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGABAT_YEAST
AccessioniPrimary (citable) accession number: P17649
Secondary accession number(s): A5H0J7, D6VUF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.