P17649 (GABAT_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-aminobutyrate aminotransferase
EC=2.6.1.19

Alternative name(s):
GABA aminotransferase
Short name=GABA-AT
Gamma-amino-N-butyrate transaminase
Short name=GABA transaminase

Gene names

Name:	UGA1
Ordered Locus Names:	YGR019W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the degradation of gamma-aminobutyric acid (GABA), which is important for utilization of GABA as nitrogen source and for oxidative stress tolerance. Deaminates GABA to succinate semialdehyde, which in turn is converted to succinate by the succinate-semialdehyde dehydrogenase UGA2. Cannot transaminate beta-alanine (BAL). Ref.2 Ref.7 Ref.11
Catalytic activity	4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer and homotetramer. Ref.2
Subcellular location	Cytoplasm Ref.8.
Induction	Subject to nitrogen catabolite repression. Expression is low in the presence of the preferred nitrogen sources, and up-regulated by GABA. Ref.11
Miscellaneous	Present with 573 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.3. Ref.7

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gamma-aminobutyric acid catabolic process Inferred from mutant phenotype Ref.7. Source: SGD nitrogen utilization Traceable author statement. Source: SGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-aminobutyrate transaminase activity Inferred from mutant phenotype Ref.7. Source: SGD pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	4-aminobutyrate aminotransferase		PRO_0000120382
Amino acid modifications
	Modified residue	90	1	Phosphoserine Ref.10
	Modified residue	326	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	240	1	H → R in CAA36833. Ref.1
	Sequence conflict	240	1	H → R in ABF58895. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P17649 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 33D446778A891F63	FASTA	471	52,946
	10 20 30 40 50 60 MSICEQYYPE EPTKPTVKTE SIPGPESQKQ LKELGEVFDT RPAYFLADYE KSLGNYITDV 70 80 90 100 110 120 DGNTYLDLYA QISSIALGYN NPALIKAAQS PEMIRALVDR PALGNFPSKD LDKILKQILK 130 140 150 160 170 180 SAPKGQDHVW SGLSGADANE LAFKAAFIYY RAKQRGYDAD FSEKENLSVM DNDAPGAPHL 190 200 210 220 230 240 AVLSFKRAFH GRLFASGSTT CSKPIHKLDF PAFHWPHAEY PSYQYPLDEN SDANRKEDDH 250 260 270 280 290 300 CLAIVEELIK TWSIPVAALI IEPIQSEGGD NHASKYFLQK LRDITLKYNV VYIIDEVQTG 310 320 330 340 350 360 VGATGKLWCH EYADIQPPVD LVTFSKKFQS AGYFFHDPKF IPNKPYRQFN TWCGEPARMI 370 380 390 400 410 420 IAGAIGQEIS DKKLTEQCSR VGDYLFKKLE GLQKKYPENF QNLRGKGRGT FIAWDLPTGE 430 440 450 460 470 KRDLLLKKLK LNGCNVGGCA VHAVRLRPSL TFEEKHADIF IEALAKSVNE L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the yeast UGA1 gene encoding GABA transaminase." Andre B., Jauniaux J.-C. Nucleic Acids Res. 18:3049-3049(1990) [PubMed: 2190186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sigma 1278B.
[2]	"A gene duplication led to specialized gamma-aminobutyrate and beta-alanine aminotransferase in yeast." Andersen G., Andersen B., Dobritzsch D., Schnackerz K.D., Piskur J. FEBS J. 274:1804-1817(2007) [PubMed: 17355287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT. Strain: ATCC 18824 / CBS 1171 / DSM 70449 / IFO 10217 / NRRL Y-12632.
[3]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae." Ramos F., El Guezzar M., Grenson M., Wiame J.-M. Eur. J. Biochem. 149:401-404(1985) [PubMed: 3888627] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[8]	"Subcellular localization of the yeast proteome." Kumar A., Agarwal S., Heyman J.A., Matson S., Heidtman M., Piccirillo S., Umansky L., Drawid A., Jansen R., Liu Y., Cheung K.-H., Miller P., Gerstein M., Roeder G.S., Snyder M. Genes Dev. 16:707-719(2002) [PubMed: 11914276] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90, MASS SPECTROMETRY.
[11]	"New insights into gamma-aminobutyric acid catabolism: evidence for gamma-hydroxybutyric acid and polyhydroxybutyrate synthesis in Saccharomyces cerevisiae." Bach B., Meudec E., Lepoutre J.-P., Rossignol T., Blondin B., Dequin S., Camarasa C. Appl. Environ. Microbiol. 75:4231-4239(2009) [PubMed: 19411412] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52600 Genomic DNA. Translation: CAA36833.1. DQ512723 Genomic DNA. Translation: ABF58895.1. Z72804 Genomic DNA. Translation: CAA97002.1. AY692904 Genomic DNA. Translation: AAT92923.1. BK006941 Genomic DNA. Translation: DAA08115.1.
PIR	S64310.
RefSeq	NP_011533.1. NM_001181148.1.
3D structure databases
ProteinModelPortal	P17649.
SMR	P17649. Positions 33-471.
ModBase	Search...
Protein-protein interaction databases
IntAct	P17649. 2 interactions.
STRING	P17649.
Proteomic databases
PeptideAtlas	P17649.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR019W; YGR019W; YGR019W.
GeneID	852902.
KEGG	sce:YGR019W.
NMPDR	fig|4932.3.peg.2643.
Organism-specific databases
CYGD	YGR019w.
SGD	S000003251. UGA1.
Phylogenomic databases
eggNOG	fuNOG06785.
GeneTree	EFGT00050000003450.
HOGENOM	HBG483813.
OMA	DANELAF.
OrthoDB	EOG4HX88H.
Enzyme and pathway databases
BioCyc	MetaCyc:YGR019W-MONOMER.
Gene expression databases
ArrayExpress	P17649.
Genevestigator	P17649.
GermOnline	YGR019W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004631. 4NH2But_aminotransferase_euk. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 2 hits.
KO	K13524.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF6. GABAtrns_euk. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00699. GABAtrns_euk. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972583.

Entry information

Entry name

GABAT_YEAST

Accession

Primary (citable) accession number: P17649
Secondary accession number(s): A5H0J7, D6VUF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program