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P17649 (GABAT_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-aminobutyrate aminotransferase

EC=2.6.1.19
Alternative name(s):
GABA aminotransferase
Short name=GABA-AT
Gamma-amino-N-butyrate transaminase
Short name=GABA transaminase
Gene names
Name:UGA1
Ordered Locus Names:YGR019W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the degradation of gamma-aminobutyric acid (GABA), which is important for utilization of GABA as nitrogen source and for oxidative stress tolerance. Deaminates GABA to succinate semialdehyde, which in turn is converted to succinate by the succinate-semialdehyde dehydrogenase UGA2. Cannot transaminate beta-alanine (BAL). Ref.2 Ref.7 Ref.10

Catalytic activity

4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.

Cofactor

Pyridoxal phosphate.

Subunit structure

Homodimer and homotetramer. Ref.2

Subcellular location

Cytoplasm Ref.8.

Induction

Subject to nitrogen catabolite repression. Expression is low in the presence of the preferred nitrogen sources, and up-regulated by GABA. Ref.10

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

Biophysicochemical properties

pH dependence:

Optimum pH is 8.3. Ref.7

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4714714-aminobutyrate aminotransferase
PRO_0000120382

Amino acid modifications

Modified residue3261N6-(pyridoxal phosphate)lysine By similarity

Experimental info

Sequence conflict2401H → R in CAA36833. Ref.1
Sequence conflict2401H → R in ABF58895. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P17649 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 33D446778A891F63

FASTA47152,946
        10         20         30         40         50         60 
MSICEQYYPE EPTKPTVKTE SIPGPESQKQ LKELGEVFDT RPAYFLADYE KSLGNYITDV 

        70         80         90        100        110        120 
DGNTYLDLYA QISSIALGYN NPALIKAAQS PEMIRALVDR PALGNFPSKD LDKILKQILK 

       130        140        150        160        170        180 
SAPKGQDHVW SGLSGADANE LAFKAAFIYY RAKQRGYDAD FSEKENLSVM DNDAPGAPHL 

       190        200        210        220        230        240 
AVLSFKRAFH GRLFASGSTT CSKPIHKLDF PAFHWPHAEY PSYQYPLDEN SDANRKEDDH 

       250        260        270        280        290        300 
CLAIVEELIK TWSIPVAALI IEPIQSEGGD NHASKYFLQK LRDITLKYNV VYIIDEVQTG 

       310        320        330        340        350        360 
VGATGKLWCH EYADIQPPVD LVTFSKKFQS AGYFFHDPKF IPNKPYRQFN TWCGEPARMI 

       370        380        390        400        410        420 
IAGAIGQEIS DKKLTEQCSR VGDYLFKKLE GLQKKYPENF QNLRGKGRGT FIAWDLPTGE 

       430        440        450        460        470 
KRDLLLKKLK LNGCNVGGCA VHAVRLRPSL TFEEKHADIF IEALAKSVNE L 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the yeast UGA1 gene encoding GABA transaminase."
Andre B., Jauniaux J.-C.
Nucleic Acids Res. 18:3049-3049(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sigma 1278B.
[2]"A gene duplication led to specialized gamma-aminobutyrate and beta-alanine aminotransferase in yeast."
Andersen G., Andersen B., Dobritzsch D., Schnackerz K.D., Piskur J.
FEBS J. 274:1804-1817(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
Strain: ATCC 18824 / CBS 1171 / DSM 70449 / IFO 10217 / NRRL Y-12632.
[3]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[7]"Mutations affecting the enzymes involved in the utilization of 4-aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiae."
Ramos F., El Guezzar M., Grenson M., Wiame J.-M.
Eur. J. Biochem. 149:401-404(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[8]"Subcellular localization of the yeast proteome."
Kumar A., Agarwal S., Heyman J.A., Matson S., Heidtman M., Piccirillo S., Umansky L., Drawid A., Jansen R., Liu Y., Cheung K.-H., Miller P., Gerstein M., Roeder G.S., Snyder M.
Genes Dev. 16:707-719(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"New insights into gamma-aminobutyric acid catabolism: evidence for gamma-hydroxybutyric acid and polyhydroxybutyrate synthesis in Saccharomyces cerevisiae."
Bach B., Meudec E., Lepoutre J.-P., Rossignol T., Blondin B., Dequin S., Camarasa C.
Appl. Environ. Microbiol. 75:4231-4239(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52600 Genomic DNA. Translation: CAA36833.1.
DQ512723 Genomic DNA. Translation: ABF58895.1.
Z72804 Genomic DNA. Translation: CAA97002.1.
AY692904 Genomic DNA. Translation: AAT92923.1.
BK006941 Genomic DNA. Translation: DAA08115.1.
PIRS64310.
RefSeqNP_011533.3. NM_001181148.3.

3D structure databases

ProteinModelPortalP17649.
SMRP17649. Positions 15-461.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33261. 36 interactions.
IntActP17649. 2 interactions.
MINTMINT-4486784.

Proteomic databases

PaxDbP17649.
PeptideAtlasP17649.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR019W; YGR019W; YGR019W.
GeneID852902.
KEGGsce:YGR019W.

Organism-specific databases

CYGDYGR019w.
SGDS000003251. UGA1.

Phylogenomic databases

eggNOGCOG0160.
GeneTreeENSGT00550000074885.
HOGENOMHOG000020208.
KOK13524.
OMANSTWGGG.
OrthoDBEOG744TK4.

Enzyme and pathway databases

BioCycMetaCyc:YGR019W-MONOMER.
YEAST:YGR019W-MONOMER.

Gene expression databases

GenevestigatorP17649.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProIPR004631. 4NH2But_aminotransferase_euk.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERPTHR11986. PTHR11986. 1 hit.
PTHR11986:SF6. PTHR11986:SF6. 1 hit.
PfamPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
TIGRFAMsTIGR00699. GABAtrns_euk. 1 hit.
PROSITEPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972583.
PROP17649.

Entry information

Entry nameGABAT_YEAST
AccessionPrimary (citable) accession number: P17649
Secondary accession number(s): A5H0J7, D6VUF4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families