Reviewed, UniProtKB/Swiss-Prot P17633 (MBHS_RHOGE)

Names and origin

Protein names

Recommended name:
    Uptake hydrogenase small subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase small subunit

Gene names

Name:	hupA
Synonyms:	hupS

Organism

Rhodocyclus gelatinosus (Rhodopseudomonas gelatinosa)

Taxonomic identifier

28068 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Rubrivivax

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.
Sequence caution	The sequence CAA36754.1 differs from that shown. Reason: Frameshift at positions 289 and 293.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 42	42	Tat-type signal Potential
	Chain	43 – 361	319	Uptake hydrogenase small subunit		PRO_0000013436
Sites
	Metal binding	59	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	62	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	156	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	190	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	228	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	231	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	256	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	262	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	271	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	290	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	293	1	Iron-sulfur 3 (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17633-1 [UniParc]. Last modified November 15, 2002. Version 2. Checksum: 2CA41EA36FC81D54	FASTA	361	39,369
	10 20 30 40 50 60 METFYEVMRR QGISRRSFLK YCSLTATSLG LAPSFVPQIA HAMETKPRTP VLWLHGLECT 70 80 90 100 110 120 CCSESFIRSA HPLAKDAVLS MISLDYDDTL MAAAGHQAEA ILDEIMAKYK GNYILAVEGN 130 140 150 160 170 180 PPLNEDGMFC IQRQALPREA QAVAADCKAV IAWGSCASWG CVQAAKPNPT QATPIHKVIT 190 200 210 220 230 240 DKPIIKVPGC PPIAEVMTGV ITYMLTFDRI PELDRQGRPK MFYSQRIHDK CYRRPHFDAG 250 260 270 280 290 300 QFVESFDDEN ARKGFCLYKV GCKGPTTYNA CSTVMWNEGT SFPIKAGHGC TGCSEDGFWD 310 320 330 340 350 360 KGSFYDRLTN IHQFGIEASA DKVGGTAAGV VGAAIAAHAA ASVIKRLSHD PDAAARAESR S

References

[1]	"Cloning and sequencing the genes encoding uptake-hydrogenase subunits of Rhodocyclus gelatinosus." Uffen R.L., Colbeau A., Richaud P., Vignais P.M. Mol. Gen. Genet. 221:49-58(1990) [PubMed: 2325631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52522 Genomic DNA. Translation: CAA36754.1. Frameshift.
PIR	S09250.
3D structure databases
HSSP	HSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 271297.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHS_RHOGE

Accession

Primary (citable) accession number: P17633

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	November 15, 2002
Last modified:	October 13, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)