Reviewed, UniProtKB/Swiss-Prot P17632 (MBHL_RHOGE)

Names and origin

Protein names

Recommended name:
    Uptake hydrogenase large subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase large subunit

Gene names

Name:	hupB
Synonyms:	hupL

Organism

Rhodocyclus gelatinosus (Rhodopseudomonas gelatinosa)

Taxonomic identifier

28068 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Rubrivivax

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 618	618	Uptake hydrogenase large subunit		PRO_0000199717
Sites
	Metal binding	75	1	Nickel Potential
	Metal binding	78	1	Nickel Potential
	Metal binding	597	1	Nickel Potential
	Metal binding	600	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	P17632-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 87B02F0338AE7BD3	FASTA	618	68,527
	10 20 30 40 50 60 MGAIETQGFK LDDSGRRIVV DPVTRIEGHM RCEVNVDANN VIRNAVSTGT MWRGLEVILK 70 80 90 100 110 120 GRDPRDAWAF VERICGVCTG CHALTSVRAV EDALGIRIPK NAHLIREMMA KTLQVHDHAV 130 140 150 160 170 180 HFYHLHALDW VDVVSALKAD PKKTSELQHL VSPSHPLSSP GYFPRRGRTG LKKFVESGQL 190 200 210 220 230 240 GPFMNGYWGS KAYVLPPEAN LMAVTHYLEA LDLQKEWVKV HAIFGGKNPH PNYLVGGVPC 250 260 270 280 290 300 AINLDGNGAA GRINMERLNF VKARIDEMIE FNKNVYLPDV LAIGTIYKQA GWLHGGGLSA 310 320 330 340 350 360 LNVADYGTYD KVAYDHATHQ LPGGVILDGN WDEIHAIDPR DPEQVQEFVA HSWYQYADES 370 380 390 400 410 420 KGLHPWDGVT EPKFELGART KGTRTAIEHI DESAKYSWIK SPRWRGHAVE VGPLSRYILG 430 440 450 460 470 480 YAHALKGNKY CQRVKEQVDF AAEAINHAIP KALGLPETQY TLKQLLPTTI GRTLARCLEG 490 500 510 520 530 540 QYCGEMMLAD YHELVANIRA GDTATANVEK WDPATWPKEA KGVGTVAAPR GMLGHWIRIK 550 560 570 580 590 600 DGKIENYQCV VPTTWNGSPR DAKGQIGAFE ASLLGTPMVN PEQPVEILRT LHSFDPCLAC 610 STHVMSEDGR ELTTVKVR

References

[1]	"Cloning and sequencing the genes encoding uptake-hydrogenase subunits of Rhodocyclus gelatinosus." Uffen R.L., Colbeau A., Richaud P., Vignais P.M. Mol. Gen. Genet. 221:49-58(1990) [PubMed: 2325631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52522 Genomic DNA. Translation: CAA36755.1.
PIR	S09251.
3D structure databases
HSSP	HSSP built from PDB template 1UBL based on UniProtKB P21852.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 271297.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHL_RHOGE

Accession

Primary (citable) accession number: P17632

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)