Reviewed, UniProtKB/Swiss-Prot P17625 (GYS2_RAT)

Names and origin

Protein names

Recommended name:
    Glycogen [starch] synthase, liver
    EC=2.4.1.11

Gene names

Name:

Gys2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Catalytic activity	UDP-glucose ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).
Enzyme regulation	Allosteric activation by glucose-6-phosphate. Phosphorylation reduces the activity towards UDP-glucose. When in the non-phosphorylated state, glycogen synthase does not require glucose-6-phosphate as an allosteric activator; when phosphorylated it does By similarity.
Pathway	Glycan biosynthesis; glycogen biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 3 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from direct assay. Source: UniProtKB response to glucose stimulus Inferred from direct assay. Source: UniProtKB
Cellular component	cortical actin cytoskeleton Inferred from direct assay. Source: UniProtKB cytosol Inferred from direct assay. Source: UniProtKB ectoplasm Inferred from direct assay. Source: UniProtKB insoluble fraction Inferred from direct assay. Source: UniProtKB soluble fraction Inferred from direct assay. Source: UniProtKB
Molecular function	glucose binding Inferred from direct assay. Source: RGD glycogen (starch) synthase activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 704	704	Glycogen [starch] synthase, liver		PRO_0000194769
Sites
	Binding site	40	1	UDP-glucose By similarity
Amino acid modifications
	Modified residue	6	1	Phosphoserine By similarity
	Modified residue	8	1	Phosphoserine; by PKA By similarity
	Modified residue	11	1	Phosphoserine By similarity
	Modified residue	626	1	Phosphothreonine By similarity
	Modified residue	641	1	Phosphoserine By similarity
	Modified residue	645	1	Phosphoserine By similarity
	Modified residue	649	1	Phosphoserine By similarity
	Modified residue	653	1	Phosphoserine By similarity
	Modified residue	657	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	82	1	E → R in AAA41255. Ref.1
	Sequence conflict	268	1	H → D in AAA41255. Ref.1
	Sequence conflict	555 – 557	3	RRF → SV in AAA41255. Ref.1
	Sequence conflict	622	1	H → Y in AAA41255. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P17625-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 926BD3057470A1D4	FASTA	704	80,734
	10 20 30 40 50 60 MLRGRSLSVT SLGGLPAWEA ERLPVEDLLL FEVSWEVTNK VGGICTVIQS KAKTTANEWG 70 80 90 100 110 120 ENYFLIGPYF EHNVKTQVEP CEPANDAVRK AVDAMNKHGC QVHFGRWLIE GSPYVVLFDI 130 140 150 160 170 180 SSSVWNLDRW KGDFWEACGV GIPHDDREAN DMLIFGSLTA WFLKEVTDHA DGKHVIAQFH 190 200 210 220 230 240 EWQAGTGLIL SRARKLPIAT IFTTHATLLG RYLCAANIDF YNQLDKFNID KEAGERQIYH 250 260 270 280 290 300 RYCMERASVH CAHVFTTVSE ITAIEADHML KRKPDVVTPN GLNVKKFSAV HEFQNLHATY 310 320 330 340 350 360 KARIQDFVRG HFYGHLDFDL EKTLFLFIAG RYEFSNKGAD IFLESLSRLN FLLRMHKSNV 370 380 390 400 410 420 TVVVFFIMPA KTNNFNVETL KGQAVRKQLW DTVHCMKEKF GKKLYDGLLR GEIPDMNSIL 430 440 450 460 470 480 DRDDLTIMKR AIFSTQRHSL PPVTTHNMID DSTDPILSTI RRIGLFNNRT DRVKVILHPE 490 500 510 520 530 540 FLSSTSPLLP MDYEEFVRGC HLGVFPSYYE PWGYTPAECT VMGIPSVTTN LSGFGCFMQE 550 560 570 580 590 600 HVADPTAYGI YIVDRRFRSP DDSCNQLTQF LYGFCKQSRR QRIIQRNRTE RLSDLLDWRY 610 620 630 640 650 660 LGRYYQHARH LTLSRAFPDK FHLEPTSPPT TDGFKYPRPS SVPPSPSGSQ TSSPQSSDVE 670 680 690 700 NEGDEDERYD EEEEAERDRL NIKSPFSLNH IPKGKKKLHG EYKN

References

[1]	"The primary structure of rat liver glycogen synthase deduced by cDNA cloning. Absence of phosphorylation sites 1a and 1b." Bai G., Zhang Z., Werner R., Nuttall F.Q., Tan A.W.H., Lee E.Y.C. J. Biol. Chem. 265:7843-7848(1990) [PubMed: 2110561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Glucose-regulated localization of liver glycogen synthase at the hepatocyte periphery." Ferrer J.C., Baque S., Guinovart J.J. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J05446 mRNA. Translation: AAA41255.1. AF346902 mRNA. Translation: AAK16592.1.
IPI	IPI00324172.
PIR	A35362.
RefSeq	NP_037221.1.
UniGene	Rn.2906
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT3. Glycosyltransferase Family 3.
PTM databases
PhosphoSite	P17625.
Genome annotation databases
GeneID	25623.
KEGG	rno:25623.
Organism-specific databases
RGD	2773. Gys2.
Phylogenomic databases
HOVERGEN	P17625.
Enzyme and pathway databases
BRENDA	2.4.1.11. 248.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	607401.

Entry information

Entry name

GYS2_RAT

Accession

Primary (citable) accession number: P17625
Secondary accession number(s): Q99MF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	December 16, 2008
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)