Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P17606

- MDHP1_SORBI

UniProt

P17606 - MDHP1_SORBI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Malate dehydrogenase [NADP] 1, chloroplastic

Gene
N/A
Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

Catalytic activityi

(S)-malate + NADP+ = oxaloacetate + NADPH.

Enzyme regulationi

Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei64 – 641Activation of NADP-MDH
Sitei69 – 691Activation of NADP-MDH
Binding sitei174 – 1741SubstratePROSITE-ProRule annotation
Binding sitei180 – 1801SubstratePROSITE-ProRule annotation
Binding sitei187 – 1871NADPBy similarity
Binding sitei194 – 1941NADPBy similarity
Binding sitei213 – 2131SubstratePROSITE-ProRule annotation
Binding sitei244 – 2441SubstratePROSITE-ProRule annotation
Active sitei269 – 2691Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi93 – 997NADPBy similarity
Nucleotide bindingi211 – 2133NADPBy similarity

GO - Molecular functioni

  1. malate dehydrogenase (NADP+) activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.82. 5768.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase [NADP] 1, chloroplastic (EC:1.1.1.82)
Alternative name(s):
NADP-MDH-1
OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
Taxonomic identifieri4558 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum

Organism-specific databases

GrameneiP17606.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4040ChloroplastBy similarityAdd
BLAST
Chaini41 – 429389Malate dehydrogenase [NADP] 1, chloroplasticPRO_0000018647Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi64 ↔ 69In oxidized inactive NAD-MDH
Disulfide bondi405 ↔ 417In oxidized inactive NAD-MDH

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1
429
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi66 – 683
Beta strandi87 – 926
Turni93 – 953
Helixi97 – 10711
Turni108 – 1125
Beta strandi118 – 1236
Helixi126 – 1283
Helixi129 – 14012
Beta strandi147 – 1548
Helixi156 – 1594
Turni160 – 1623
Beta strandi164 – 1685
Helixi180 – 20122
Beta strandi207 – 2104
Beta strandi212 – 2143
Helixi215 – 22410
Helixi231 – 2333
Beta strandi234 – 2363
Helixi239 – 25214
Helixi257 – 2593
Beta strandi264 – 2674
Beta strandi274 – 2763
Helixi287 – 2893
Helixi294 – 30613
Helixi308 – 3158
Helixi321 – 33616
Beta strandi345 – 3506
Beta strandi360 – 36910
Beta strandi372 – 3743
Helixi386 – 40520
Helixi407 – 4104
Beta strandi412 – 4143

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
7MDHX-ray2.40A/B/C/D55-429[»]
ProteinModelPortaliP17606.
SMRiP17606. Positions 61-426.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17606.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00051.
OMAiFPDYRHA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17606-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLSTAYSPV GSHLAPAPLG HRRSAQLHRP RRALLATVRC SVDAAKQVQD
60 70 80 90 100
GVATAEAPAT RKDCFGVFCT TYDLKAEDKT KSWKKLVNIA VSGAAGMISN
110 120 130 140 150
HLLFKLASGE VFGQDQPIAL KLLGSERSFQ ALEGVAMELE DSLYPLLREV
160 170 180 190 200
SIGIDPYEVF EDVDWALLIG AKPRGPGMER AALLDINGQI FADQGKALNA
210 220 230 240 250
VASKNVKVLV VGNPCNTNAL ICLKNAPDIP AKNFHALTRL DENRAKCQLA
260 270 280 290 300
LKAGVFYDKV SNVTIWGNHS TTQVPDFLNA KIDGRPVKEV IKDTKWLEEE
310 320 330 340 350
FTITVQKRGG ALIQKWGRSS AASTAVSIAD AIKSLVTPTP EGDWFSTGVY
360 370 380 390 400
TTGNPYGIAE DIVFSMPCRS KGDGDYELAT DVSMDDFLWE RIKKSEAELL
410 420
AEKKCVAHLT GEGNAYCDVP EDTMLPGEV
Length:429
Mass (Da):46,455
Last modified:August 1, 1990 - v1
Checksum:i17D9E0A34A67F1FF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti226 – 2283APD → PPH in CAA37531. (PubMed:2209586)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31965 Genomic DNA. Translation: AAA34047.1.
X53453 mRNA. Translation: CAA37531.1.
PIRiJH0151.
S13588.
RefSeqiXP_002445670.1. XM_002445625.1.
UniGeneiSbi.76.

Genome annotation databases

GeneIDi8057802.
KEGGisbi:SORBI_07g023920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31965 Genomic DNA. Translation: AAA34047.1 .
X53453 mRNA. Translation: CAA37531.1 .
PIRi JH0151.
S13588.
RefSeqi XP_002445670.1. XM_002445625.1.
UniGenei Sbi.76.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
7MDH X-ray 2.40 A/B/C/D 55-429 [» ]
ProteinModelPortali P17606.
SMRi P17606. Positions 61-426.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8057802.
KEGGi sbi:SORBI_07g023920.

Organism-specific databases

Gramenei P17606.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000220953.
KOi K00051.
OMAi FPDYRHA.

Enzyme and pathway databases

BRENDAi 1.1.1.82. 5768.

Miscellaneous databases

EvolutionaryTracei P17606.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure and characterization of the Sorghum vulgare gene encoding NADP-malate dehydrogenase."
    Luchetta P., Cretin C., Gadal P.
    Gene 89:171-177(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. INRA 450.
    Tissue: Leaf.
  2. "Primary structure of sorghum malate dehydrogenase (NADP) deduced from cDNA sequence. Homology with malate dehydrogenase (NAD)."
    Cretin C., Luchetta P., Joly C., Decottignies P., Lepiniec L., Gadal P., Sallantin M., Huet J.-C., Pernollet J.-C.
    Eur. J. Biochem. 192:299-303(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Tamaran FNK 140.
    Tissue: Leaf.
  3. "Structural basis for light activation of a chloroplast enzyme: the structure of sorghum NADP-malate dehydrogenase in its oxidized form."
    Johansson K., Ramaswamy S., Saarinen M., Lemaire-Chamley M., Issakidis-Bourguet E., Miginiac-Maslow M., Eklund H.
    Biochemistry 38:4319-4326(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiMDHP1_SORBI
AccessioniPrimary (citable) accession number: P17606
Secondary accession number(s): P19796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3