Reviewed, UniProtKB/Swiss-Prot P17599 (SYN1_BOVIN)

Names and origin

Protein names

Recommended name:
    Synapsin-1
Alternative name(s):
    Synapsin I

Gene names

Name:

SYN1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level By similarity.
Subunit structure	Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ib interacts with PRNP By similarity.
Subcellular location	Cell junction › synapse. Golgi apparatus By similarity.
Post-translational modification	Substrate of at least four different protein kinases. Phosphorylation may play a role in the regulation of synapsin-1 in the nerve terminal.
Sequence similarities	Belongs to the synapsin family.

Ontologies

Keywords
Cellular component	Cell junction Golgi apparatus Synapse
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Actin-binding
PTM	Glycoprotein Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	neurotransmitter secretion Inferred from sequence or structural similarity. Source: AgBase
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cell junction Inferred from electronic annotation. Source: UniProtKB-KW synaptic vesicle membrane Inferred from sequence or structural similarity. Source: AgBase synaptonemal complex Inferred from sequence or structural similarity. Source: AgBase
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro actin binding Inferred from electronic annotation. Source: UniProtKB-KW catalytic activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform IA (identifier: P17599-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform IB (identifier: P17599-2)

The sequence of this isoform differs from the canonical sequence as follows:
     662-670: NKSQSLTNA → KASPAQAQP
     671-706: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 706

706

Synapsin-1

PRO_0000183016

Regions

Region

1 – 28

28

A

Region

29 – 112

84

B; linker

Region

113 – 420

308

C; actin-binding and synaptic-vesicle binding

Region

421 – 656

236

D; Pro-rich linker

Region

657 – 706

50

E

Amino acid modifications

Modified residue

9

1

Phosphoserine; by CaMK1 and PKA

Modified residue

39

1

Phosphoserine By similarity

Modified residue

62

1

Phosphoserine By similarity

Modified residue

67

1

Phosphoserine By similarity

Modified residue

312

1

Phosphotyrosine By similarity

Modified residue

337

1

Phosphothreonine By similarity

Modified residue

427

1

Phosphoserine By similarity

Modified residue

510

1

Phosphoserine By similarity

Modified residue

512

1

Phosphothreonine By similarity

Modified residue

520

1

Phosphoserine By similarity

Modified residue

551

1

Phosphoserine; by PDPK Ref.2

Modified residue

553

1

Phosphoserine By similarity

Modified residue

568

1

Phosphoserine; by CaMK2

Modified residue

605

1

Phosphoserine; by CaMK2

Modified residue

666

1

Phosphoserine By similarity

Modified residue

705

1

Phosphoserine By similarity

Glycosylation

55

1

O-linked (GlcNAc) By similarity

Glycosylation

87

1

O-linked (GlcNAc) By similarity

Glycosylation

96

1

O-linked (GlcNAc) By similarity

Glycosylation

103

1

O-linked (GlcNAc) By similarity

Glycosylation

261

1

O-linked (GlcNAc) By similarity

Glycosylation

432

1

O-linked (GlcNAc) By similarity

Glycosylation

526

1

O-linked (GlcNAc) By similarity

Glycosylation

564

1

O-linked (GlcNAc) By similarity

Glycosylation

578

1

O-linked (GlcNAc) By similarity

Natural variations

Alternative sequence

662 – 670

9

NKSQSLTNA → KASPAQAQP in isoform IB.

VSP_006314

Alternative sequence

671 – 706

36

Missing in isoform IB.

VSP_006315

Secondary structure

1

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706

Helix Strand Turn

Details...

Beta strand

114 – 119

6

Helix

126 – 130

5

Turn

136 – 138

3

Beta strand

141 – 146

6

Helix

148 – 150

3

Beta strand

151 – 156

6

Beta strand

161 – 169

9

Turn

170 – 172

3

Beta strand

173 – 178

6

Beta strand

181 – 185

5

Beta strand

189 – 191

3

Turn

192 – 195

4

Helix

199 – 207

9

Beta strand

212 – 214

3

Helix

216 – 221

6

Helix

225 – 239

15

Turn

241 – 243

3

Beta strand

250 – 254

5

Helix

255 – 257

3

Beta strand

262 – 272

11

Turn

275 – 278

4

Beta strand

279 – 282

4

Helix

285 – 296

12

Turn

297 – 299

3

Beta strand

302 – 306

5

Beta strand

310 – 319

10

Beta strand

322 – 328

7

Beta strand

345 – 347

3

Helix

351 – 360

10

Helix

361 – 365

5

Beta strand

368 – 377

10

Beta strand

382 – 388

7

Helix

399 – 415

17

Sequences

	Sequence		Length	Mass (Da)
	Isoform IA [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 89373750BF014340	FASTA	706	74,518
	10 20 30 40 50 60 MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPAAPSP GATTGPATAT AERASSAAPV 70 80 90 100 110 120 ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG GGSGGAGRGG AAARVLLVID 130 140 150 160 170 180 EPHTDWAKYF KGKKIHGEID IKVEQAEFSD LNLVAHANGG FSVDMEVLRN GVKVVRSLKP 190 200 210 220 230 240 DFVLIRQHAF SMARNGDYRS LVIGLQYAGI PSINSLHSVY NFCDKPWVFA QMVRLHKKLG 250 260 270 280 290 300 TEEFPLINQT FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 310 320 330 340 350 360 YATTEPFIDA KYDVRIQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS DRYKLWVDTC 370 380 390 400 410 420 SEIFGGLDIC AVEALHGKDG RDHIIQVVGS SMPLIGDHQD EDKQLIVELV VNKMAQALPR 430 440 450 460 470 480 QRQRDASPGR GSHSQTPSPG ALPLGRQISQ QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP 490 500 510 520 530 540 LQQRPTPQGQ QHLSGLGPPA GSPLPQRLPS PTSVPQQPAS QATPMTQGQG RQSRPVAGGP 550 560 570 580 590 600 GAPPATRPPA SPSPQRQAGP PQATRQTSVS GQAPPKASGV PPGGQQRQGP PQKPPGPAGP 610 620 630 640 650 660 TRQASQAGPM PRTGPPTTQQ PRPSGPGPAG RPTKPQLAQK PSQDVPPPAT AAAGGPPHPQ 670 680 690 700 LNKSQSLTNA FNLPEPAPPR PSLSQDEVKA ETIRSLRKSF ASLFSD
	Isoform IB. Checksum: D330C4C4E23E2ED1	FASTA	670	70,439
	10 20 30 40 50 60 MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPAAPSP GATTGPATAT AERASSAAPV 70 80 90 100 110 120 ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG GGSGGAGRGG AAARVLLVID 130 140 150 160 170 180 EPHTDWAKYF KGKKIHGEID IKVEQAEFSD LNLVAHANGG FSVDMEVLRN GVKVVRSLKP 190 200 210 220 230 240 DFVLIRQHAF SMARNGDYRS LVIGLQYAGI PSINSLHSVY NFCDKPWVFA QMVRLHKKLG 250 260 270 280 290 300 TEEFPLINQT FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 310 320 330 340 350 360 YATTEPFIDA KYDVRIQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS DRYKLWVDTC 370 380 390 400 410 420 SEIFGGLDIC AVEALHGKDG RDHIIQVVGS SMPLIGDHQD EDKQLIVELV VNKMAQALPR 430 440 450 460 470 480 QRQRDASPGR GSHSQTPSPG ALPLGRQISQ QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP 490 500 510 520 530 540 LQQRPTPQGQ QHLSGLGPPA GSPLPQRLPS PTSVPQQPAS QATPMTQGQG RQSRPVAGGP 550 560 570 580 590 600 GAPPATRPPA SPSPQRQAGP PQATRQTSVS GQAPPKASGV PPGGQQRQGP PQKPPGPAGP 610 620 630 640 650 660 TRQASQAGPM PRTGPPTTQQ PRPSGPGPAG RPTKPQLAQK PSQDVPPPAT AAAGGPPHPQ 670 LKASPAQAQP

References

[1]	"Synapsins: mosaics of shared and individual domains in a family of synaptic vesicle phosphoproteins." Suedhof T.C., Czernik A.J., Kao H.-T., Takei K., Johnston P.A., Horiuchi A., Kanazir S.D., Wagner M.A., Perin M.S., de Camilli P., Greengard P. Science 245:1474-1480(1989) [PubMed: 2506642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS IA AND IB). Tissue: Brain.
[2]	"Phosphorylation of synapsin I at a novel site by proline-directed protein kinase." Hall F.L., Mitchell J.P., Vulliet P.R. J. Biol. Chem. 265:6944-6948(1990) [PubMed: 2108963] [Abstract] Cited for: PHOSPHORYLATION AT SER-551 BY PDPK.
[3]	"Synapsin I is structurally similar to ATP-utilizing enzymes." Esser L., Wang C.-R., Hosaka M., Smagula C.S., Suedhof T.C., Deisenhofer J. EMBO J. 17:977-984(1998) [PubMed: 9463376] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 112-417.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M27810 mRNA. Translation: AAA30761.1.
M27811 mRNA. Translation: AAA30762.1.

IPI

IPI00705569.
IPI00705843.

PIR

E30411.

RefSeq

NP_776616.1.

UniGene

Bt.62979

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AUV	X-ray	2.15	A/B	110-420	[»]
1AUX	X-ray	2.30	A/B	110-420	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P17599.

PTM databases

GlycoSuiteDB

P17599.

Genome annotation databases

Ensembl

ENSBTAT00000027503; ENSBTAP00000027503; ENSBTAG00000005042; Bos taurus. [Genome view]

GeneID

281510.

KEGG

bta:281510.

Organism-specific databases

CTD

281510.

Phylogenomic databases

eggNOG

maNOG18102.

HOVERGEN

P17599.

Family and domain databases

InterPro

IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp-like.
IPR001359. Synapsin.
IPR019735. Synapsin_CS.
IPR019569. Synapsin_N.
IPR019736. Synapsin_P_site.
[Graphical view]

Gene3D

G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.

Pfam

PF10581. Synapsin_N. 1 hit.
[Graphical view]

PRINTS

PR01368. SYNAPSIN.

PROSITE

PS00415. SYNAPSIN_1. 1 hit.
PS00416. SYNAPSIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SYN1_BOVIN

Accession

Primary (citable) accession number: P17599

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	February 1, 1996
Last modified:	January 19, 2010
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)