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P17599

- SYN1_BOVIN

UniProt

P17599 - SYN1_BOVIN

Protein

Synapsin-1

Gene

SYN1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. catalytic activity Source: InterPro

    GO - Biological processi

    1. neurotransmitter secretion Source: AgBase

    Keywords - Ligandi

    Actin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synapsin-1
    Alternative name(s):
    Synapsin I
    Gene namesi
    Name:SYN1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. Golgi apparatus Source: UniProtKB-SubCell
    3. synapse Source: AgBase
    4. synaptic vesicle Source: AgBase
    5. synaptic vesicle membrane Source: AgBase
    6. synaptonemal complex Source: AgBase

    Keywords - Cellular componenti

    Cell junction, Golgi apparatus, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 706706Synapsin-1PRO_0000183016Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei9 – 91Phosphoserine; by CaMK1 and PKABy similarity
    Glycosylationi55 – 551O-linked (GlcNAc)By similarity
    Glycosylationi87 – 871O-linked (GlcNAc)By similarity
    Glycosylationi96 – 961O-linked (GlcNAc)By similarity
    Glycosylationi103 – 1031O-linked (GlcNAc)By similarity
    Glycosylationi261 – 2611O-linked (GlcNAc)By similarity
    Modified residuei312 – 3121PhosphotyrosineBy similarity
    Modified residuei427 – 4271PhosphoserineBy similarity
    Glycosylationi432 – 4321O-linked (GlcNAc)By similarity
    Glycosylationi526 – 5261O-linked (GlcNAc)By similarity
    Modified residuei551 – 5511Phosphoserine; by PDPK11 Publication
    Modified residuei553 – 5531PhosphoserineBy similarity
    Glycosylationi564 – 5641O-linked (GlcNAc)By similarity
    Modified residuei568 – 5681Phosphoserine; by CaMK21 Publication
    Glycosylationi578 – 5781O-linked (GlcNAc)By similarity
    Modified residuei605 – 6051Phosphoserine; by CaMK21 Publication

    Post-translational modificationi

    Substrate of at least four different protein kinases. Phosphorylation may play a role in the regulation of synapsin-1 in the nerve terminal.2 Publications
    Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP17599.

    PTM databases

    UniCarbKBiP17599.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ib interacts with PRNP By similarity.By similarity

    Protein-protein interaction databases

    MINTiMINT-139117.

    Structurei

    Secondary structure

    1
    706
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi114 – 1196
    Helixi126 – 1305
    Turni136 – 1383
    Beta strandi141 – 1466
    Helixi148 – 1503
    Beta strandi151 – 1555
    Beta strandi161 – 1699
    Turni170 – 1723
    Beta strandi173 – 1786
    Beta strandi181 – 1855
    Beta strandi189 – 1913
    Turni192 – 1954
    Helixi199 – 2079
    Beta strandi212 – 2143
    Helixi216 – 2216
    Helixi225 – 23915
    Turni241 – 2433
    Beta strandi250 – 2545
    Helixi255 – 2573
    Beta strandi262 – 27211
    Turni275 – 2784
    Beta strandi279 – 2824
    Helixi285 – 29612
    Turni297 – 2993
    Beta strandi302 – 3065
    Beta strandi310 – 31910
    Beta strandi322 – 3287
    Beta strandi345 – 3473
    Helixi351 – 36010
    Helixi361 – 3655
    Beta strandi368 – 37710
    Beta strandi382 – 3887
    Helixi399 – 41517

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AUVX-ray2.15A/B110-420[»]
    1AUXX-ray2.30A/B110-420[»]
    ProteinModelPortaliP17599.
    SMRiP17599. Positions 112-417.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP17599.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2828AAdd
    BLAST
    Regioni29 – 11284B; linkerAdd
    BLAST
    Regioni113 – 420308C; actin-binding and synaptic-vesicle bindingAdd
    BLAST
    Regioni421 – 656236D; Pro-rich linkerAdd
    BLAST
    Regioni657 – 70650EAdd
    BLAST

    Domaini

    The A region binds phospholipids with a preference for negatively charged species.By similarity

    Sequence similaritiesi

    Belongs to the synapsin family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG284201.
    HOGENOMiHOG000231323.
    HOVERGENiHBG016354.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    InterProiIPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR028713. SYN1.
    IPR001359. Synapsin.
    IPR020898. Synapsin_ATP-bd_dom.
    IPR019735. Synapsin_CS.
    IPR019736. Synapsin_P_site.
    IPR020897. Synapsin_pre-ATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR10841. PTHR10841. 1 hit.
    PTHR10841:SF8. PTHR10841:SF8. 1 hit.
    PfamiPF02078. Synapsin. 1 hit.
    PF02750. Synapsin_C. 1 hit.
    PF10581. Synapsin_N. 1 hit.
    [Graphical view]
    PRINTSiPR01368. SYNAPSIN.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    PROSITEiPS00415. SYNAPSIN_1. 1 hit.
    PS00416. SYNAPSIN_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform IA (identifier: P17599-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNYLRRRLSD SNFMANLPNG YMTDLQRPQP PPPPPAAPSP GATTGPATAT    50
    AERASSAAPV ASPAAPSPGS SGGGGFFSSL SNAVKQTTAA AAATFSEQVG 100
    GGSGGAGRGG AAARVLLVID EPHTDWAKYF KGKKIHGEID IKVEQAEFSD 150
    LNLVAHANGG FSVDMEVLRN GVKVVRSLKP DFVLIRQHAF SMARNGDYRS 200
    LVIGLQYAGI PSINSLHSVY NFCDKPWVFA QMVRLHKKLG TEEFPLINQT 250
    FYPNHKEMLS STTYPVVVKM GHAHSGMGKV KVDNQHDFQD IASVVALTKT 300
    YATTEPFIDA KYDVRIQKIG QNYKAYMRTS VSGNWKTNTG SAMLEQIAMS 350
    DRYKLWVDTC SEIFGGLDIC AVEALHGKDG RDHIIQVVGS SMPLIGDHQD 400
    EDKQLIVELV VNKMAQALPR QRQRDASPGR GSHSQTPSPG ALPLGRQISQ 450
    QPAGPPAQQR PPPQGGPPQP GPGPQRQGPP LQQRPTPQGQ QHLSGLGPPA 500
    GSPLPQRLPS PTSVPQQPAS QATPMTQGQG RQSRPVAGGP GAPPATRPPA 550
    SPSPQRQAGP PQATRQTSVS GQAPPKASGV PPGGQQRQGP PQKPPGPAGP 600
    TRQASQAGPM PRTGPPTTQQ PRPSGPGPAG RPTKPQLAQK PSQDVPPPAT 650
    AAAGGPPHPQ LNKSQSLTNA FNLPEPAPPR PSLSQDEVKA ETIRSLRKSF 700
    ASLFSD 706
    Length:706
    Mass (Da):74,518
    Last modified:February 1, 1996 - v2
    Checksum:i89373750BF014340
    GO
    Isoform IB (identifier: P17599-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         662-670: NKSQSLTNA → KASPAQAQP
         671-706: Missing.

    Show »
    Length:670
    Mass (Da):70,439
    Checksum:iD330C4C4E23E2ED1
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei662 – 6709NKSQSLTNA → KASPAQAQP in isoform IB. 1 PublicationVSP_006314
    Alternative sequencei671 – 70636Missing in isoform IB. 1 PublicationVSP_006315Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27810 mRNA. Translation: AAA30761.1.
    M27811 mRNA. Translation: AAA30762.1.
    PIRiE30411.
    RefSeqiNP_776616.1. NM_174191.2. [P17599-1]
    UniGeneiBt.62979.

    Genome annotation databases

    GeneIDi281510.
    KEGGibta:281510.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27810 mRNA. Translation: AAA30761.1 .
    M27811 mRNA. Translation: AAA30762.1 .
    PIRi E30411.
    RefSeqi NP_776616.1. NM_174191.2. [P17599-1 ]
    UniGenei Bt.62979.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AUV X-ray 2.15 A/B 110-420 [» ]
    1AUX X-ray 2.30 A/B 110-420 [» ]
    ProteinModelPortali P17599.
    SMRi P17599. Positions 112-417.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-139117.

    PTM databases

    UniCarbKBi P17599.

    Proteomic databases

    PRIDEi P17599.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281510.
    KEGGi bta:281510.

    Organism-specific databases

    CTDi 6853.

    Phylogenomic databases

    eggNOGi NOG284201.
    HOGENOMi HOG000231323.
    HOVERGENi HBG016354.

    Miscellaneous databases

    EvolutionaryTracei P17599.
    NextBioi 20805477.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 2 hits.
    3.40.50.20. 1 hit.
    InterProi IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR028713. SYN1.
    IPR001359. Synapsin.
    IPR020898. Synapsin_ATP-bd_dom.
    IPR019735. Synapsin_CS.
    IPR019736. Synapsin_P_site.
    IPR020897. Synapsin_pre-ATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR10841. PTHR10841. 1 hit.
    PTHR10841:SF8. PTHR10841:SF8. 1 hit.
    Pfami PF02078. Synapsin. 1 hit.
    PF02750. Synapsin_C. 1 hit.
    PF10581. Synapsin_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01368. SYNAPSIN.
    SUPFAMi SSF52440. SSF52440. 1 hit.
    PROSITEi PS00415. SYNAPSIN_1. 1 hit.
    PS00416. SYNAPSIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Synapsins: mosaics of shared and individual domains in a family of synaptic vesicle phosphoproteins."
      Suedhof T.C., Czernik A.J., Kao H.-T., Takei K., Johnston P.A., Horiuchi A., Kanazir S.D., Wagner M.A., Perin M.S., de Camilli P., Greengard P.
      Science 245:1474-1480(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS IA AND IB).
      Tissue: Brain.
    2. "Amino acid sequences surrounding the cAMP-dependent and calcium/calmodulin-dependent phosphorylation sites in rat and bovine synapsin I."
      Czernik A.J., Pang D.T., Greengard P.
      Proc. Natl. Acad. Sci. U.S.A. 84:7518-7522(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-568 AND SER-605.
    3. "Phosphorylation of synapsin I at a novel site by proline-directed protein kinase."
      Hall F.L., Mitchell J.P., Vulliet P.R.
      J. Biol. Chem. 265:6944-6948(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-551 BY PDPK.
    4. "Synapsin I is structurally similar to ATP-utilizing enzymes."
      Esser L., Wang C.-R., Hosaka M., Smagula C.S., Suedhof T.C., Deisenhofer J.
      EMBO J. 17:977-984(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 112-417.

    Entry informationi

    Entry nameiSYN1_BOVIN
    AccessioniPrimary (citable) accession number: P17599
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3