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Protein

Fumarate reductase iron-sulfur subunit

Gene

frdB

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The fumarate reductase enzyme complex is required for fumarate respiration using formate or sulfide as electron donor.1 Publication

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Iron-sulfur 1 (2Fe-2S)
Metal bindingi62 – 621Iron-sulfur 1 (2Fe-2S)
Metal bindingi65 – 651Iron-sulfur 1 (2Fe-2S)
Metal bindingi77 – 771Iron-sulfur 1 (2Fe-2S)
Metal bindingi151 – 1511Iron-sulfur 2 (4Fe-4S)
Metal bindingi154 – 1541Iron-sulfur 2 (4Fe-4S)
Metal bindingi157 – 1571Iron-sulfur 2 (4Fe-4S)
Metal bindingi161 – 1611Iron-sulfur 3 (3Fe-4S)
Metal bindingi208 – 2081Iron-sulfur 3 (3Fe-4S)
Metal bindingi214 – 2141Iron-sulfur 3 (3Fe-4S)
Metal bindingi218 – 2181Iron-sulfur 2 (4Fe-4S)

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  4. electron carrier activity Source: InterPro
  5. metal ion binding Source: UniProtKB-KW
  6. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate reductase iron-sulfur subunit (EC:1.3.5.1)
Gene namesi
Name:frdB
Ordered Locus Names:WS0830
OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Taxonomic identifieri273121 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
ProteomesiUP000000422 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Fumarate reductase iron-sulfur subunitPRO_0000158708Add
BLAST

Interactioni

Subunit structurei

Part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein, and cytochrome b-556.

Protein-protein interaction databases

STRINGi273121.WS0830.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 118Combined sources
Beta strandi22 – 298Combined sources
Helixi36 – 4611Combined sources
Beta strandi56 – 627Combined sources
Beta strandi66 – 694Combined sources
Beta strandi72 – 754Combined sources
Helixi76 – 783Combined sources
Helixi81 – 833Combined sources
Beta strandi87 – 926Combined sources
Beta strandi97 – 1015Combined sources
Beta strandi104 – 1063Combined sources
Helixi108 – 11710Combined sources
Beta strandi130 – 1323Combined sources
Helixi139 – 14911Combined sources
Helixi156 – 1594Combined sources
Helixi162 – 1665Combined sources
Helixi172 – 18312Combined sources
Helixi192 – 1998Combined sources
Turni202 – 2043Combined sources
Helixi205 – 2073Combined sources
Helixi213 – 2175Combined sources
Helixi224 – 23512Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E7PX-ray3.10B/E/H/K1-239[»]
1QLBX-ray2.33B/E1-239[»]
2BS2X-ray1.78B/E1-239[»]
2BS3X-ray2.19B/E1-239[»]
2BS4X-ray2.76B/E1-239[»]
SMRiP17596. Positions 1-239.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17596.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 95912Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini142 – 171304Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0479.
HOGENOMiHOG000160590.
KOiK00245.
OMAiNTGDWFL.
OrthoDBiEOG6CS02W.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
PF13183. Fer4_8. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRMLTIRVF KYDPQSAVSK PHFQEYKIEE APSMTIFIVL NMIRETYDPD
60 70 80 90 100
LNFDFVCRAG ICGSCGMMIN GRPSLACRTL TKDFEDGVIT LLPLPAFKLI
110 120 130 140 150
KDLSVDTGNW FNGMSQRVES WIHAQKEHDI SKLEERIEPE VAQEVFELDR
160 170 180 190 200
CIECGCCIAA CGTKIMREDF VGAAGLNRVV RFMIDPHDER TDEDYYELIG
210 220 230
DDDGVFGCMT LLACHDVCPK NLPLQSKIAY LRRKMVSVN
Length:239
Mass (Da):27,165
Last modified:July 31, 1990 - v1
Checksum:i24D1992489B8FFD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000662 Genomic DNA. Translation: CAA04215.1.
BX571659 Genomic DNA. Translation: CAE09941.1.
PIRiC44954.
RefSeqiNP_907041.1. NC_005090.1.
WP_011138738.1. NC_005090.1.

Genome annotation databases

KEGGiwsu:WS0830.
PATRICi24038706. VBIWolSuc63014_0782.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000662 Genomic DNA. Translation: CAA04215.1.
BX571659 Genomic DNA. Translation: CAE09941.1.
PIRiC44954.
RefSeqiNP_907041.1. NC_005090.1.
WP_011138738.1. NC_005090.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E7PX-ray3.10B/E/H/K1-239[»]
1QLBX-ray2.33B/E1-239[»]
2BS2X-ray1.78B/E1-239[»]
2BS3X-ray2.19B/E1-239[»]
2BS4X-ray2.76B/E1-239[»]
SMRiP17596. Positions 1-239.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273121.WS0830.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiwsu:WS0830.
PATRICi24038706. VBIWolSuc63014_0782.

Phylogenomic databases

eggNOGiCOG0479.
HOGENOMiHOG000160590.
KOiK00245.
OMAiNTGDWFL.
OrthoDBiEOG6CS02W.

Miscellaneous databases

EvolutionaryTraceiP17596.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
PF13183. Fer4_8. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The fumarate reductase operon of Wolinella succinogenes. Sequence and expression of the frdA and frdB genes."
    Lauterbach F., Koertner C., Albracht S.P., Unden G., Kroeger A.
    Arch. Microbiol. 154:386-393(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Deletion and site-directed mutagenesis of the Wolinella succinogenes fumarate reductase operon."
    Simon J., Gross R., Ringel M., Schmidt E., Kroeger A.
    Eur. J. Biochem. 251:418-426(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.
  4. "Structure of fumarate reductase from Wolinella succinogenes at 2.2 A resolution."
    Lancaster C.R.D., Kroeger A., Auer M., Michel H.
    Nature 402:377-385(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
  5. "A third crystal form of Wolinella succinogenes quinol:fumarate reductase reveals domain closure at the site of fumarate reduction."
    Lancaster C.R.D., Gross R., Simon J.
    Eur. J. Biochem. 268:1820-1827(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).

Entry informationi

Entry nameiFRDB_WOLSU
AccessioniPrimary (citable) accession number: P17596
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1990
Last sequence update: July 31, 1990
Last modified: March 31, 2015
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.