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P17596

- FRDB_WOLSU

UniProt

P17596 - FRDB_WOLSU

Protein

Fumarate reductase iron-sulfur subunit

Gene

frdB

Organism
Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    The fumarate reductase enzyme complex is required for fumarate respiration using formate or sulfide as electron donor.1 Publication

    Catalytic activityi

    Succinate + a quinone = fumarate + a quinol.

    Cofactori

    Binds 1 2Fe-2S cluster.
    Binds 1 3Fe-4S cluster.
    Binds 1 4Fe-4S cluster.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Iron-sulfur 1 (2Fe-2S)
    Metal bindingi62 – 621Iron-sulfur 1 (2Fe-2S)
    Metal bindingi65 – 651Iron-sulfur 1 (2Fe-2S)
    Metal bindingi77 – 771Iron-sulfur 1 (2Fe-2S)
    Metal bindingi151 – 1511Iron-sulfur 2 (4Fe-4S)
    Metal bindingi154 – 1541Iron-sulfur 2 (4Fe-4S)
    Metal bindingi157 – 1571Iron-sulfur 2 (4Fe-4S)
    Metal bindingi161 – 1611Iron-sulfur 3 (3Fe-4S)
    Metal bindingi208 – 2081Iron-sulfur 3 (3Fe-4S)
    Metal bindingi214 – 2141Iron-sulfur 3 (3Fe-4S)
    Metal bindingi218 – 2181Iron-sulfur 2 (4Fe-4S)

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    4. electron carrier activity Source: InterPro
    5. metal ion binding Source: UniProtKB-KW
    6. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport, Tricarboxylic acid cycle

    Keywords - Ligandi

    2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fumarate reductase iron-sulfur subunit (EC:1.3.5.1)
    Gene namesi
    Name:frdB
    Ordered Locus Names:WS0830
    OrganismiWolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W) (Vibrio succinogenes)
    Taxonomic identifieri273121 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella
    ProteomesiUP000000422: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. respiratory chain Source: UniProtKB-KW

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 239239Fumarate reductase iron-sulfur subunitPRO_0000158708Add
    BLAST

    Interactioni

    Subunit structurei

    Part of an enzyme complex containing three subunits: a flavoprotein, an iron-sulfur protein, and cytochrome b-556.

    Protein-protein interaction databases

    STRINGi273121.WS0830.

    Structurei

    Secondary structure

    1
    239
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 118
    Beta strandi22 – 298
    Helixi36 – 4611
    Beta strandi56 – 627
    Beta strandi66 – 694
    Beta strandi72 – 754
    Helixi76 – 783
    Helixi81 – 833
    Beta strandi87 – 926
    Beta strandi97 – 1015
    Beta strandi104 – 1063
    Helixi108 – 11710
    Beta strandi130 – 1323
    Helixi139 – 14911
    Helixi156 – 1594
    Helixi162 – 1665
    Helixi172 – 18312
    Helixi192 – 1998
    Turni202 – 2043
    Helixi205 – 2073
    Helixi213 – 2175
    Helixi224 – 23512

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E7PX-ray3.10B/E/H/K1-239[»]
    1QLBX-ray2.33B/E1-239[»]
    2BS2X-ray1.78B/E1-239[»]
    2BS3X-ray2.19B/E1-239[»]
    2BS4X-ray2.76B/E1-239[»]
    ProteinModelPortaliP17596.
    SMRiP17596. Positions 1-239.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP17596.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 95912Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini142 – 171304Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0479.
    HOGENOMiHOG000160590.
    KOiK00245.
    OMAiFMIDPHD.
    OrthoDBiEOG6CS02W.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR012675. Beta-grasp_dom.
    IPR009051. Helical_ferredxn.
    IPR004489. Succ_DH/fum_Rdtase_Fe-S.
    IPR025192. Succ_DH/fum_Rdtase_N.
    [Graphical view]
    PfamiPF13085. Fer2_3. 1 hit.
    PF13183. Fer4_8. 1 hit.
    [Graphical view]
    SUPFAMiSSF46548. SSF46548. 1 hit.
    SSF54292. SSF54292. 1 hit.
    TIGRFAMsiTIGR00384. dhsB. 1 hit.
    PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17596-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRMLTIRVF KYDPQSAVSK PHFQEYKIEE APSMTIFIVL NMIRETYDPD    50
    LNFDFVCRAG ICGSCGMMIN GRPSLACRTL TKDFEDGVIT LLPLPAFKLI 100
    KDLSVDTGNW FNGMSQRVES WIHAQKEHDI SKLEERIEPE VAQEVFELDR 150
    CIECGCCIAA CGTKIMREDF VGAAGLNRVV RFMIDPHDER TDEDYYELIG 200
    DDDGVFGCMT LLACHDVCPK NLPLQSKIAY LRRKMVSVN 239
    Length:239
    Mass (Da):27,165
    Last modified:August 1, 1990 - v1
    Checksum:i24D1992489B8FFD1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000662 Genomic DNA. Translation: CAA04215.1.
    BX571659 Genomic DNA. Translation: CAE09941.1.
    PIRiC44954.
    RefSeqiNP_907041.1. NC_005090.1.
    WP_011138738.1. NC_005090.1.

    Genome annotation databases

    EnsemblBacteriaiCAE09941; CAE09941; WS0830.
    GeneIDi2555194.
    KEGGiwsu:WS0830.
    PATRICi24038706. VBIWolSuc63014_0782.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ000662 Genomic DNA. Translation: CAA04215.1 .
    BX571659 Genomic DNA. Translation: CAE09941.1 .
    PIRi C44954.
    RefSeqi NP_907041.1. NC_005090.1.
    WP_011138738.1. NC_005090.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E7P X-ray 3.10 B/E/H/K 1-239 [» ]
    1QLB X-ray 2.33 B/E 1-239 [» ]
    2BS2 X-ray 1.78 B/E 1-239 [» ]
    2BS3 X-ray 2.19 B/E 1-239 [» ]
    2BS4 X-ray 2.76 B/E 1-239 [» ]
    ProteinModelPortali P17596.
    SMRi P17596. Positions 1-239.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273121.WS0830.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE09941 ; CAE09941 ; WS0830 .
    GeneIDi 2555194.
    KEGGi wsu:WS0830.
    PATRICi 24038706. VBIWolSuc63014_0782.

    Phylogenomic databases

    eggNOGi COG0479.
    HOGENOMi HOG000160590.
    KOi K00245.
    OMAi FMIDPHD.
    OrthoDBi EOG6CS02W.

    Miscellaneous databases

    EvolutionaryTracei P17596.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR012675. Beta-grasp_dom.
    IPR009051. Helical_ferredxn.
    IPR004489. Succ_DH/fum_Rdtase_Fe-S.
    IPR025192. Succ_DH/fum_Rdtase_N.
    [Graphical view ]
    Pfami PF13085. Fer2_3. 1 hit.
    PF13183. Fer4_8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46548. SSF46548. 1 hit.
    SSF54292. SSF54292. 1 hit.
    TIGRFAMsi TIGR00384. dhsB. 1 hit.
    PROSITEi PS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The fumarate reductase operon of Wolinella succinogenes. Sequence and expression of the frdA and frdB genes."
      Lauterbach F., Koertner C., Albracht S.P., Unden G., Kroeger A.
      Arch. Microbiol. 154:386-393(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Deletion and site-directed mutagenesis of the Wolinella succinogenes fumarate reductase operon."
      Simon J., Gross R., Ringel M., Schmidt E., Kroeger A.
      Eur. J. Biochem. 251:418-426(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W.
    4. "Structure of fumarate reductase from Wolinella succinogenes at 2.2 A resolution."
      Lancaster C.R.D., Kroeger A., Auer M., Michel H.
      Nature 402:377-385(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
    5. "A third crystal form of Wolinella succinogenes quinol:fumarate reductase reveals domain closure at the site of fumarate reduction."
      Lancaster C.R.D., Gross R., Simon J.
      Eur. J. Biochem. 268:1820-1827(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).

    Entry informationi

    Entry nameiFRDB_WOLSU
    AccessioniPrimary (citable) accession number: P17596
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3