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Protein

D-2-hydroxyisocaproate dehydrogenase

Gene
N/A
Organism
Lactobacillus paracasei
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NAD dependent reversible, stereospecific interconversion between 2-ketocarboxylic acids and D-2-hydroxy-carboxylic acids.

Miscellaneous

Can be applied in an industrial process for the production of D-amino acid.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei175NADCombined sources1 Publication1
Binding sitei205NAD; via carbonyl oxygenCombined sources1 Publication1
Binding sitei211NADCombined sources1 Publication1
Active sitei234By similarity1
Binding sitei258NADCombined sources1 Publication1
Active sitei263By similarity1
Active sitei295Proton donorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi155 – 156NADCombined sources1 Publication2
Nucleotide bindingi205 – 206NADBy similarity2
Nucleotide bindingi232 – 234NADCombined sources1 Publication3

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Names & Taxonomyi

Protein namesi
Recommended name:
D-2-hydroxyisocaproate dehydrogenase (EC:1.1.1.-)
Short name:
D-HICDH
OrganismiLactobacillus paracasei
Taxonomic identifieri1597 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Pathology & Biotechi

Chemistry databases

DrugBankiDB03229 2-Oxo-4-Methylpentanoic Acid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000759401 – 333D-2-hydroxyisocaproate dehydrogenaseAdd BLAST333

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1333
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Turni10 – 12Combined sources3
Helixi13 – 23Combined sources11
Beta strandi26 – 29Combined sources4
Helixi38 – 42Combined sources5
Beta strandi46 – 50Combined sources5
Helixi58 – 66Combined sources9
Beta strandi71 – 77Combined sources7
Helixi84 – 89Combined sources6
Beta strandi93 – 95Combined sources3
Helixi102 – 117Combined sources16
Helixi120 – 128Combined sources9
Helixi132 – 135Combined sources4
Helixi143 – 145Combined sources3
Beta strandi146 – 151Combined sources6
Helixi155 – 166Combined sources12
Beta strandi170 – 174Combined sources5
Helixi191 – 197Combined sources7
Beta strandi199 – 203Combined sources5
Helixi209 – 211Combined sources3
Helixi217 – 222Combined sources6
Beta strandi227 – 231Combined sources5
Helixi240 – 248Combined sources9
Beta strandi251 – 259Combined sources9
Helixi263 – 273Combined sources11
Helixi279 – 285Combined sources7
Beta strandi290 – 292Combined sources3
Helixi301 – 321Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DXYX-ray1.86A1-333[»]
ProteinModelPortaliP17584
SMRiP17584
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17584

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiView protein in InterPro
IPR006139 D-isomer_2_OHA_DH_cat_dom
IPR029753 D-isomer_DH_CS
IPR029752 D-isomer_DH_CS1
IPR006140 D-isomer_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00389 2-Hacid_dh, 1 hit
PF02826 2-Hacid_dh_C, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00065 D_2_HYDROXYACID_DH_1, 1 hit
PS00670 D_2_HYDROXYACID_DH_2, 1 hit
PS00671 D_2_HYDROXYACID_DH_3, 1 hit

Sequencei

Sequence statusi: Complete.

P17584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIIAYGARV DEIQYFKQWA KDTGNTLEYH TEFLDENTVE WAKGFDGINS
60 70 80 90 100
LQTTPYAAGV FEKMHAYGIK FLTIRNVGTD NIDMTAMKQY GIRLSNVPAY
110 120 130 140 150
SPAAIAEFAL TDTLYLLRNM GKVQAQLQAG DYEKAGTFIG KELGQQTVGV
160 170 180 190 200
MGTGHIGQVA IKLFKGFGAK VIAYDPYPMK GDHPDFDYVS LEDLFKQSDV
210 220 230 240 250
IDLHVPGIEQ NTHIINEAAF NLMKPGAIVI NTARPNLIDT QAMLSNLKSG
260 270 280 290 300
KLAGVGIDTY EYETEDLLNL AKHGSFKDPL WDELLGMPNV VLSPHIAYYT
310 320 330
ETAVHNMVYF SLQHLVDFLT KGETSTEVTG PAK
Length:333
Mass (Da):36,893
Last modified:November 1, 1997 - v2
Checksum:i40C165872884328B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti322 – 333GETST…TGPAK → FKPARKLLVQQVVN (PubMed:2504649).CuratedAdd BLAST12

Mass spectrometryi

Molecular mass is 36880 Da from positions 1 - 333. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26929 Genomic DNA Translation: AAA25236.1
PIRiJU0050 DELBC
RefSeqiWP_003577354.1, NZ_NOKL01000002.1

Similar proteinsi

Entry informationi

Entry nameiDHD2_LACPA
AccessioniPrimary (citable) accession number: P17584
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing
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Main funding by: National Institutes of Health