Reviewed, UniProtKB/Swiss-Prot P17570 (NIA_SOLLC)

Names and origin

Protein names

Recommended name:
    Nitrate reductase [NADH]
      Short name=NR
    EC=1.7.1.1

Gene names

Name:

NIA

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	911 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activity	Nitrite + NAD+ + H2O = nitrate + NADH.
Cofactor	Binds 1 FAD. Binds 1 heme group. The heme group is called cytochrome b-557. Binds 1 molybdenum ion.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the nitrate reductase family. Contains 1 cytochrome b5 heme-binding domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Nitrate assimilation
Ligand	FAD Flavoprotein Heme Iron Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 911	911	Nitrate reductase [NADH]		PRO_0000166061
Regions
	Domain	536 – 611	76	Cytochrome b5 heme-binding
	Domain	654 – 766	113	FAD-binding FR-type
Sites
	Metal binding	188	1	Molybdenum-pterin Potential
	Metal binding	242	1	Molybdenum-pterin Potential
	Metal binding	571	1	Iron (heme axial ligand) By similarity
	Metal binding	594	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Disulfide bond	427		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P17570-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: DE8736181F02A0D5	FASTA	911	102,453
	10 20 30 40 50 60 MAASVENRQY THLEPGLSGV GRTFKPRPDS PVRGCNFPPS SNHELPFQKK QNPPIYLDYS 70 80 90 100 110 120 SSEDEDDDDE KNEYVQMIKK GKTELEPSIH DTRDEGTADN WIERNFSLIR LTGKHPFNSE 130 140 150 160 170 180 PPLSRLMHHG FITPVPLHYV RNHGPVPKAS WSDWTVEVTG LVKRPMKFTM DQLVNEFPSR 190 200 210 220 230 240 EFPVTLVCAG NRRKEQNMVK QTIGFNWGAA AVSTTVWRGV PLRALLKRCG VQSKKKGALN 250 260 270 280 290 300 VCFEGSDVLP GGGGSKYGTS IKKEFAMDPS RDIIVAYMQN GEMLSPDHGF PVRMIIPGFI 310 320 330 340 350 360 GGRMVKWLKR IVVTTQESES YYHYKDNRVL PPHVDAELAN AEAWWYKPEY IINELNINSV 370 380 390 400 410 420 ITTPCHEEIL PINAWTTQRP YTLRGYAYSG GGKKVTRVEV TLDGGETWSV CTLDHPEKPT 430 440 450 460 470 480 KYGKYWCWCF WSLEVEVLDL LSAKEIAVRA TDETLNTQPE KLIWNVMGMM NNCWFRVKMN 490 500 510 520 530 540 VCKPHKGEIG IVFEHPTQPG NQSGGWMAKE RHLEISAVAP PTLKKSISTP FMNTASKMYS 550 560 570 580 590 600 MSEVRKHNSS DSAWIIVHGH IYDASRFLKD HPGGVDSILI NAGTDCTEEF DAIHSDKAKK 610 620 630 640 650 660 LLEDFRIGEL ITTGYTSDSS PNSSVHGSSS ISSFLAPIKE LVQTPTRSVA LIPREKIPCK 670 680 690 700 710 720 LVDKQSISHD VRKFKFALPS EDQVLGLPVG KHIFLCATVD DKLCMRAYTP TSTVDEVGFF 730 740 750 760 770 780 ELVVKIYFKG VHPKFPNGGQ MSQHLDSLPI GAFLDVKGPL GHIEYQGKGN FLVHGKQKFA 790 800 810 820 830 840 KKLAMIAGGT GITPVYQVMQ SILKDPEDDT EMYVVYANRT EDDILLKDEL DAWAEQVPNR 850 860 870 880 890 900 VKVWYVVQES ITQGWKYSTG FVTESILREH IPEPSHTTLA LACGPPPMIQ FAINPNLEKM 910 GYDIKEELLV F

References

[1]

"Cloning and analysis of the tomato nitrate reductase-encoding gene: protein domain structure and amino acid homologies in higher plants." Daniel-Vedele F., Dorbe M.F., Caboche M., Rouze P. Gene 85:371-380(1989) [PubMed: 2628174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Manapal. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X14060 Genomic DNA. Translation: CAA32218.1.
PIR	RDTONH. JQ0373.
3D structure databases
HSSP	HSSP built from PDB template 2CND based on UniProtKB P17571.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.1.1. 281054.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR005066. MoCF_OxRdtse_dimer. IPR008335. Mopterin_OxRdtase_euk. IPR001834. NADH-Cyt_B5_reductase. IPR012137. Nitr_rd_NADH. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:2.60.40.650. MoCF_oxrdtse_dimer. 1 hit. G3DSA:3.90.420.10. Oxred_molyb_bd. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00970. FAD_binding_6. 1 hit. PF03404. Mo-co_dimer. 1 hit. PF00175. NAD_binding_1. 1 hit. PF00174. Oxidored_molyb. 1 hit. [Graphical view]
PIRSF	PIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTS	PR00406. CYTB5RDTASE. PR00363. CYTOCHROMEB5. PR00407. EUMOPTERIN. PR00371. FPNCR.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS51384. FAD_FR. 1 hit. PS00559. MOLYBDOPTERIN_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIA_SOLLC

Accession

Primary (citable) accession number: P17570

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)