P17556 (DHA_LYSSH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine dehydrogenase
EC=1.4.1.1

Gene names

Name:

ald

Organism

Lysinibacillus sphaericus (Bacillus sphaericus)

Taxonomic identifier

1421 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Lysinibacillus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme is a key factor in the assimilation of L-alanine as an energy source through the tricarboxylic acid cycle during sporulation.
Catalytic activity	L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.
Pathway	Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.
Subunit structure	Homohexamer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the AlaDH/PNT family.
Biophysicochemical properties	Temperature dependence: Loses about 50% of its initial activity when heated at 65 degrees Celsius for 5 min.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alanine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Alanine dehydrogenase		PRO_0000198990
Regions
	Nucleotide binding	170 – 200	31	NAD By similarity
Sites
	Active site	96	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P17556 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 5E6FD5E4D93AA98B	FASTA	372	39,460
	10 20 30 40 50 60 MKIGIPKEIK NNENRVAMTP AGVVSLTHAG HERLAIETGG GIGSSFTDAE YVAAGAAYRC 70 80 90 100 110 120 IGKEAWAQEM ILKVKEPVAS EYDYFYEGQI LFTYLHLAPR AELTQALIDK KVVGIAYETV 130 140 150 160 170 180 QLANGSLPLL TPMSEVAGKM ATQIGAQYLE KNHGGKGILL GGVSGVHARK VTVIGGGIAG 190 200 210 220 230 240 TNAAKIAVGM GADVTVIDLS PERLRQLEDM FGRDVQTLMS NPYNIAESVK HSDLVVGAVL 250 260 270 280 290 300 IPGAKAPKLV SEEMIQSMQP GSVVVDIAID QGGIFATSDR VTTHDDPTYV KHGVVHYAVA 310 320 330 340 350 360 NMPGAVPRTS TIALTNNTIP YALQIANKGY KQACIDNPAL KKGVNALEGH ITYKAVAEAQ 370 GLPYVNVDEL IQ

References

[1]

"Alanine dehydrogenases from two Bacillus species with distinct thermostabilities: molecular cloning, DNA and protein sequence determination, and structural comparison with other NAD(P)(+)-dependent dehydrogenases." Kuroda S., Tanizawa K., Sakamoto Y., Tanaka H., Soda K. Biochemistry 29:1009-1015(1990) [PubMed: 2340274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 10208 / NRS 966 / NCIB 11935 / 1911.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33298 Genomic DNA. Translation: AAA22210.1.
PIR	A34261.
3D structure databases
ProteinModelPortal	P17556.
SMR	P17556. Positions 1-371.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008142. Ala_DH/PNT_CS1. IPR008143. Ala_DH/PNT_CS2. IPR008141. Ala_DH/Pyr_transHydgase. IPR007886. AlaDH/PNT_N. IPR007698. AlaDH/PNT_NAD(H)-bd. [Graphical view]
PANTHER	PTHR10160:SF3. PTHR10160:SF3. 1 hit.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
PIRSF	PIRSF000183. Alanine_dh. 1 hit.
SMART	SM01002. AlaDh_PNT_C. 1 hit. SM01003. AlaDh_PNT_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00518. AlaDH. 1 hit.
PROSITE	PS00836. ALADH_PNT_1. 1 hit. PS00837. ALADH_PNT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHA_LYSSH

Accession

Primary (citable) accession number: P17556

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program