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P17555 (CAP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenylyl cyclase-associated protein

Short name=CAP
Gene names
Name:SRV2
Synonyms:CAP1
Ordered Locus Names:YNL138W
ORF Names:N1210, N1838
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The N-terminal domain binds to adenylyl cyclase, thereby enabling adenylyl cyclase to be activated by upstream regulatory signals, such as Ras. The C-terminal domain is required for normal cellular morphology and growth control.

Subunit structure

Homodimer.

Subcellular location

Cytoplasmcytoskeletonactin patch. Note: Cortical actin patches. Ref.6

Miscellaneous

Present with 8760 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CAP family.

Contains 1 C-CAP/cofactor C-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 526526Adenylyl cyclase-associated protein
PRO_0000205706

Regions

Domain369 – 504136C-CAP/cofactor C-like
Region1 – 168168Adenyl cyclase-binding
Region354 – 3618Interaction with SH3 domain of ABP1
Region370 – 526157Dimerization and actin-binding
Motif169 – 369201SH3-binding
Compositional bias262 – 30039Ala/Pro/Ser-rich
Compositional bias277 – 2826Poly-Pro

Amino acid modifications

Modified residue4541Phosphoserine Ref.8

Secondary structure

................................. 526
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P17555 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 0EB4D41205E2D464

FASTA52657,521
        10         20         30         40         50         60 
MPDSKYTMQG YNLVKLLKRL EEATARLEDV TIYQEGYIQN KLEASKNNKP SDSGADANTT 

        70         80         90        100        110        120 
NEPSAENAPE VEQDPKCITA FQSYIGENID PLVELSGKID TVVLDALQLL KGGFQSQLTF 

       130        140        150        160        170        180 
LRAAVRSRKP DYSSQTFADS LRPINENIIK LGQLKESNRQ SKYFAYLSAL SEGAPLFSWV 

       190        200        210        220        230        240 
AVDTPVSMVT DFKDAAQFWT NRILKEYRES DPNAVEWVKK FLASFDNLKA YIKEYHTTGV 

       250        260        270        280        290        300 
SWKKDGMDFA DAMAQSTKNT GATSSPSPAS ATAAPAPPPP PPAPPASVFE ISNDTPATSS 

       310        320        330        340        350        360 
DANKGGIGAV FAELNQGENI TKGLKKVDKS QQTHKNPELR QSSTVSSTGS KSGPPPRPKK 

       370        380        390        400        410        420 
PSTLKTKRPP RKELVGNKWF IENYENETES LVIDANKDES IFIGKCSQVL VQIKGKVNAI 

       430        440        450        460        470        480 
SLSETESCSV VLDSSISGMD VIKSNKFGIQ VNHSLPQISI DKSDGGNIYL SKESLNTEIY 

       490        500        510        520 
TSCSTAINVN LPIGEDDDYV EFPIPEQMKH SFADGKFKSA VFEHAG 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein."
Field J., Vojtek A., Ballester R., Bolger G., Colicelli J., Ferguson K., Gerst J., Kataoka T., Michaeli T., Powers S., Riggs M., Rodgers L., Wieland I., Wheland B., Wigler M.
Cell 61:319-327(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"SRV2, a gene required for RAS activation of adenylate cyclase in yeast."
Fedor-Chaiken M., Deschenes R.J., Broach J.R.
Cell 61:329-340(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
Mallet L., Bussereau F., Jacquet M.
Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"A conserved proline-rich region of the Saccharomyces cerevisiae cyclase-associated protein binds SH3 domains and modulates cytoskeletal localization."
Freeman N.L., Lila T., Mintzer K.A., Chen Z., Pahk A.J., Ren R., Drubin D.G., Field J.
Mol. Cell. Biol. 16:548-556(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ABP1, SUBCELLULAR LOCATION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-454, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Crystal structure of the actin binding domain of the cyclase-associated protein."
Dodatko T., Fedorov A.A., Grynberg M., Patskovsky Y., Rozwarski D.A., Jaroszewski L., Aronoff-Spencer E., Kondraskina E., Irving T., Godzik A., Almo S.C.
Biochemistry 43:10628-10641(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 368-526.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M58284 mRNA. Translation: AAA63569.1.
M32663 Genomic DNA. Translation: AAA35094.1.
Z46843 Genomic DNA. Translation: CAA86887.1.
Z71414 Genomic DNA. Translation: CAA96020.1.
BK006947 Genomic DNA. Translation: DAA10410.1.
PIRA34896.
RefSeqNP_014261.1. NM_001182976.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K4ZX-ray2.30A/B369-526[»]
1KQ5X-ray3.00A/B369-526[»]
ProteinModelPortalP17555.
SMRP17555. Positions 78-252, 369-524.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35688. 67 interactions.
DIPDIP-77N.
IntActP17555. 37 interactions.
MINTMINT-582270.
STRING4932.YNL138W.

Proteomic databases

PaxDbP17555.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL138W; YNL138W; YNL138W.
GeneID855584.
KEGGsce:YNL138W.

Organism-specific databases

CYGDYNL138w.
SGDS000005082. SRV2.

Phylogenomic databases

eggNOGNOG254262.
GeneTreeENSGT00390000017955.
HOGENOMHOG000206192.
KOK17261.
OMAERAMPEM.
OrthoDBEOG7H79BV.

Enzyme and pathway databases

BioCycYEAST:G3O-33157-MONOMER.

Gene expression databases

GenevestigatorP17555.

Family and domain databases

Gene3D2.160.20.70. 1 hit.
InterProIPR001837. Adenylate_cyclase-assoc_CAP.
IPR013912. Adenylate_cyclase-assoc_CAP_C.
IPR013992. Adenylate_cyclase-assoc_CAP_N.
IPR017901. C-CAP_CF_C-like.
IPR016098. CAP/MinC_C.
IPR028417. CAP_CS_C.
IPR018106. CAP_CS_N.
IPR028419. CAP_fungal/plant.
IPR006599. CARP_motif.
[Graphical view]
PANTHERPTHR10652. PTHR10652. 1 hit.
PTHR10652:SF0. PTHR10652:SF0. 1 hit.
PfamPF08603. CAP_C. 1 hit.
PF01213. CAP_N. 1 hit.
[Graphical view]
SMARTSM00673. CARP. 2 hits.
[Graphical view]
SUPFAMSSF101278. SSF101278. 2 hits.
SSF69340. SSF69340. 1 hit.
PROSITEPS51329. C_CAP_COFACTOR_C. 1 hit.
PS01088. CAP_1. 1 hit.
PS01089. CAP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP17555.
NextBio979715.

Entry information

Entry nameCAP_YEAST
AccessionPrimary (citable) accession number: P17555
Secondary accession number(s): D6W144
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 16, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references