Adenylyl cyclase-associated protein - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P17555 (CAP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 124. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Adenylyl cyclase-associated protein
Short name=CAP

Gene names

Name:	SRV2
Synonyms:	CAP1
Ordered Locus Names:	YNL138W
ORF Names:	N1210, N1838

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The N-terminal domain binds to adenylyl cyclase, thereby enabling adenylyl cyclase to be activated by upstream regulatory signals, such as Ras. The C-terminal domain is required for normal cellular morphology and growth control.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm › cytoskeleton › actin patch. Note: Cortical actin patches. Ref.6
Miscellaneous	Present with 8760 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CAP family. Contains 1 C-CAP/cofactor C-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Ras protein signal transduction Inferred from mutant phenotype PubMed 9774417. Source: SGD cell morphogenesis Inferred from electronic annotation. Source: InterPro cytoskeleton organization Traceable author statement Ref.6. Source: SGD
Cellular_component	actin cortical patch Inferred from direct assay Ref.6. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD
Molecular_function	adenylate cyclase binding Traceable author statement Ref.6. Source: SGD cytoskeletal protein binding Traceable author statement Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
itself		3	EBI-4024,EBI-4024
ABP1	P15891	5	EBI-4024,EBI-2036
ACT1	P60010	5	EBI-4024,EBI-2169
RPL3	P14126	3	EBI-4024,EBI-15364
TEF2	P02994	3	EBI-4024,EBI-6314

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 526

526

Adenylyl cyclase-associated protein

PRO_0000205706

Regions

Domain

369 – 504

136

C-CAP/cofactor C-like

Region

1 – 168

168

Adenyl cyclase-binding

Region

354 – 361

Interaction with SH3 domain of ABP1

Region

370 – 526

157

Dimerization and actin-binding

Motif

169 – 369

201

SH3-binding

Compositional bias

262 – 300

Ala/Pro/Ser-rich

Compositional bias

277 – 282

Poly-Pro

Amino acid modifications

Modified residue

454

Phosphoserine Ref.8

Secondary structure

526

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P17555 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 0EB4D41205E2D464
Show »

FASTA

526

57,521

        10         20         30         40         50         60 
MPDSKYTMQG YNLVKLLKRL EEATARLEDV TIYQEGYIQN KLEASKNNKP SDSGADANTT 

        70         80         90        100        110        120 
NEPSAENAPE VEQDPKCITA FQSYIGENID PLVELSGKID TVVLDALQLL KGGFQSQLTF 

       130        140        150        160        170        180 
LRAAVRSRKP DYSSQTFADS LRPINENIIK LGQLKESNRQ SKYFAYLSAL SEGAPLFSWV 

       190        200        210        220        230        240 
AVDTPVSMVT DFKDAAQFWT NRILKEYRES DPNAVEWVKK FLASFDNLKA YIKEYHTTGV 

       250        260        270        280        290        300 
SWKKDGMDFA DAMAQSTKNT GATSSPSPAS ATAAPAPPPP PPAPPASVFE ISNDTPATSS 

       310        320        330        340        350        360 
DANKGGIGAV FAELNQGENI TKGLKKVDKS QQTHKNPELR QSSTVSSTGS KSGPPPRPKK 

       370        380        390        400        410        420 
PSTLKTKRPP RKELVGNKWF IENYENETES LVIDANKDES IFIGKCSQVL VQIKGKVNAI 

       430        440        450        460        470        480 
SLSETESCSV VLDSSISGMD VIKSNKFGIQ VNHSLPQISI DKSDGGNIYL SKESLNTEIY 

       490        500        510        520 
TSCSTAINVN LPIGEDDDYV EFPIPEQMKH SFADGKFKSA VFEHAG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein." Field J., Vojtek A., Ballester R., Bolger G., Colicelli J., Ferguson K., Gerst J., Kataoka T., Michaeli T., Powers S., Riggs M., Rodgers L., Wieland I., Wheland B., Wigler M. Cell 61:319-327(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"SRV2, a gene required for RAS activation of adenylate cyclase in yeast." Fedor-Chaiken M., Deschenes R.J., Broach J.R. Cell 61:329-340(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames." Mallet L., Bussereau F., Jacquet M. Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"A conserved proline-rich region of the Saccharomyces cerevisiae cyclase-associated protein binds SH3 domains and modulates cytoskeletal localization." Freeman N.L., Lila T., Mintzer K.A., Chen Z., Pahk A.J., Ren R., Drubin D.G., Field J. Mol. Cell. Biol. 16:548-556(1996) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ABP1, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-454, MASS SPECTROMETRY.
[9]	"Crystal structure of the actin binding domain of the cyclase-associated protein." Dodatko T., Fedorov A.A., Grynberg M., Patskovsky Y., Rozwarski D.A., Jaroszewski L., Aronoff-Spencer E., Kondraskina E., Irving T., Godzik A., Almo S.C. Biochemistry 43:10628-10641(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 368-526.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M58284 mRNA. Translation: AAA63569.1.
M32663 Genomic DNA. Translation: AAA35094.1.
Z46843 Genomic DNA. Translation: CAA86887.1.
Z71414 Genomic DNA. Translation: CAA96020.1.
BK006947 Genomic DNA. Translation: DAA10410.1.

PIR

A34896.

RefSeq

NP_014261.1. NM_001182976.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K4Z	X-ray	2.30	A/B	369-526	[»]
1KQ5	X-ray	3.00	A/B	369-526	[»]

ProteinModelPortal

P17555.

SMR

P17555. Positions 78-252, 369-524.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-77N.

IntAct

P17555. 40 interactions.

MINT

MINT-582270.

STRING

4932.YNL138W.

Proteomic databases

PaxDb

P17555.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YNL138W; YNL138W; YNL138W.

GeneID

855584.

KEGG

sce:YNL138W.

Organism-specific databases

CYGD

YNL138w.

SGD

S000005082. SRV2.

Phylogenomic databases

eggNOG

NOG254262.

GeneTree

ENSGT00390000017955.

HOGENOM

HOG000206192.

OMA

LFENEGR.

OrthoDB

EOG4K9FND.

Gene expression databases

Genevestigator

P17555.

GermOnline

YNL138W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

2.160.20.70. 1 hit.

InterPro

IPR001837. Adenylate_cyclase-assoc_CAP.
IPR013912. Adenylate_cyclase-assoc_CAP_C.
IPR013992. Adenylate_cyclase-assoc_CAP_N.
IPR017901. C-CAP_CF_C-like.
IPR016098. CAP/MinC_C.
IPR018106. CAP_CS.
IPR006599. CARP_motif.
[Graphical view]

PANTHER

PTHR10652. PTHR10652. 1 hit.

Pfam

PF08603. CAP_C. 1 hit.
PF01213. CAP_N. 1 hit.
[Graphical view]

SMART

SM00673. CARP. 2 hits.
[Graphical view]

SUPFAM

SSF101278. Adenylate_cyclase-assoc_CAP_N. 1 hit.
SSF69340. CARP. 1 hit.

PROSITE

PS51329. C_CAP_COFACTOR_C. 1 hit.
PS01088. CAP_1. 1 hit.
PS01089. CAP_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P17555.

NextBio

979715.

Entry information

Entry name

CAP_YEAST

Accession

Primary (citable) accession number: P17555
Secondary accession number(s): D6W144

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents