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P17555

- CAP_YEAST

UniProt

P17555 - CAP_YEAST

Protein

Adenylyl cyclase-associated protein

Gene

SRV2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    The N-terminal domain binds to adenylyl cyclase, thereby enabling adenylyl cyclase to be activated by upstream regulatory signals, such as Ras. The C-terminal domain is required for normal cellular morphology and growth control.

    GO - Molecular functioni

    1. actin binding Source: SGD
    2. adenylate cyclase binding Source: SGD
    3. identical protein binding Source: IntAct
    4. protein binding Source: IntAct

    GO - Biological processi

    1. actin filament organization Source: SGD
    2. actin filament severing Source: SGD
    3. cell morphogenesis Source: InterPro
    4. Ras protein signal transduction Source: SGD

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33157-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylyl cyclase-associated protein
    Short name:
    CAP
    Gene namesi
    Name:SRV2
    Synonyms:CAP1
    Ordered Locus Names:YNL138W
    ORF Names:N1210, N1838
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL138w.
    SGDiS000005082. SRV2.

    Subcellular locationi

    Cytoplasmcytoskeletonactin patch 1 Publication
    Note: Cortical actin patches.

    GO - Cellular componenti

    1. actin cortical patch Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 526526Adenylyl cyclase-associated proteinPRO_0000205706Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei454 – 4541Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP17555.
    PaxDbiP17555.

    Expressioni

    Gene expression databases

    GenevestigatoriP17555.

    Interactioni

    Subunit structurei

    Homodimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-4024,EBI-4024
    ABP1P158919EBI-4024,EBI-2036
    ACT1P600105EBI-4024,EBI-2169
    RPL3P141263EBI-4024,EBI-15364
    TEF2P029943EBI-4024,EBI-6314

    Protein-protein interaction databases

    BioGridi35688. 67 interactions.
    DIPiDIP-77N.
    IntActiP17555. 37 interactions.
    MINTiMINT-582270.
    STRINGi4932.YNL138W.

    Structurei

    Secondary structure

    1
    526
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi371 – 3755
    Beta strandi378 – 3825
    Beta strandi391 – 3944
    Beta strandi400 – 41516
    Beta strandi417 – 43317
    Beta strandi435 – 4373
    Beta strandi439 – 45315
    Beta strandi456 – 4627
    Beta strandi464 – 4696
    Turni473 – 4764
    Beta strandi478 – 4836
    Beta strandi485 – 4928
    Helixi495 – 4973
    Beta strandi500 – 5034
    Beta strandi508 – 5136
    Beta strandi516 – 5216

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K4ZX-ray2.30A/B369-526[»]
    1KQ5X-ray3.00A/B369-526[»]
    ProteinModelPortaliP17555.
    SMRiP17555. Positions 78-252, 369-524.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP17555.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini369 – 504136C-CAP/cofactor C-likePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 168168Adenyl cyclase-bindingAdd
    BLAST
    Regioni354 – 3618Interaction with SH3 domain of ABP1
    Regioni370 – 526157Dimerization and actin-bindingAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi169 – 369201SH3-bindingAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi262 – 30039Ala/Pro/Ser-richAdd
    BLAST
    Compositional biasi277 – 2826Poly-Pro

    Sequence similaritiesi

    Belongs to the CAP family.Curated
    Contains 1 C-CAP/cofactor C-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG254262.
    GeneTreeiENSGT00390000017955.
    HOGENOMiHOG000206192.
    KOiK17261.
    OMAiAQGGQTR.
    OrthoDBiEOG7H79BV.

    Family and domain databases

    Gene3Di2.160.20.70. 1 hit.
    InterProiIPR001837. Adenylate_cyclase-assoc_CAP.
    IPR013912. Adenylate_cyclase-assoc_CAP_C.
    IPR013992. Adenylate_cyclase-assoc_CAP_N.
    IPR017901. C-CAP_CF_C-like.
    IPR016098. CAP/MinC_C.
    IPR028417. CAP_CS_C.
    IPR018106. CAP_CS_N.
    IPR028419. CAP_fungal/plant.
    IPR006599. CARP_motif.
    [Graphical view]
    PANTHERiPTHR10652. PTHR10652. 1 hit.
    PTHR10652:SF0. PTHR10652:SF0. 1 hit.
    PfamiPF08603. CAP_C. 1 hit.
    PF01213. CAP_N. 1 hit.
    [Graphical view]
    SMARTiSM00673. CARP. 2 hits.
    [Graphical view]
    SUPFAMiSSF101278. SSF101278. 2 hits.
    SSF69340. SSF69340. 1 hit.
    PROSITEiPS51329. C_CAP_COFACTOR_C. 1 hit.
    PS01088. CAP_1. 1 hit.
    PS01089. CAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17555-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPDSKYTMQG YNLVKLLKRL EEATARLEDV TIYQEGYIQN KLEASKNNKP    50
    SDSGADANTT NEPSAENAPE VEQDPKCITA FQSYIGENID PLVELSGKID 100
    TVVLDALQLL KGGFQSQLTF LRAAVRSRKP DYSSQTFADS LRPINENIIK 150
    LGQLKESNRQ SKYFAYLSAL SEGAPLFSWV AVDTPVSMVT DFKDAAQFWT 200
    NRILKEYRES DPNAVEWVKK FLASFDNLKA YIKEYHTTGV SWKKDGMDFA 250
    DAMAQSTKNT GATSSPSPAS ATAAPAPPPP PPAPPASVFE ISNDTPATSS 300
    DANKGGIGAV FAELNQGENI TKGLKKVDKS QQTHKNPELR QSSTVSSTGS 350
    KSGPPPRPKK PSTLKTKRPP RKELVGNKWF IENYENETES LVIDANKDES 400
    IFIGKCSQVL VQIKGKVNAI SLSETESCSV VLDSSISGMD VIKSNKFGIQ 450
    VNHSLPQISI DKSDGGNIYL SKESLNTEIY TSCSTAINVN LPIGEDDDYV 500
    EFPIPEQMKH SFADGKFKSA VFEHAG 526
    Length:526
    Mass (Da):57,521
    Last modified:August 1, 1990 - v1
    Checksum:i0EB4D41205E2D464
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58284 mRNA. Translation: AAA63569.1.
    M32663 Genomic DNA. Translation: AAA35094.1.
    Z46843 Genomic DNA. Translation: CAA86887.1.
    Z71414 Genomic DNA. Translation: CAA96020.1.
    BK006947 Genomic DNA. Translation: DAA10410.1.
    PIRiA34896.
    RefSeqiNP_014261.1. NM_001182976.1.

    Genome annotation databases

    EnsemblFungiiYNL138W; YNL138W; YNL138W.
    GeneIDi855584.
    KEGGisce:YNL138W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58284 mRNA. Translation: AAA63569.1 .
    M32663 Genomic DNA. Translation: AAA35094.1 .
    Z46843 Genomic DNA. Translation: CAA86887.1 .
    Z71414 Genomic DNA. Translation: CAA96020.1 .
    BK006947 Genomic DNA. Translation: DAA10410.1 .
    PIRi A34896.
    RefSeqi NP_014261.1. NM_001182976.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K4Z X-ray 2.30 A/B 369-526 [» ]
    1KQ5 X-ray 3.00 A/B 369-526 [» ]
    ProteinModelPortali P17555.
    SMRi P17555. Positions 78-252, 369-524.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35688. 67 interactions.
    DIPi DIP-77N.
    IntActi P17555. 37 interactions.
    MINTi MINT-582270.
    STRINGi 4932.YNL138W.

    Proteomic databases

    MaxQBi P17555.
    PaxDbi P17555.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL138W ; YNL138W ; YNL138W .
    GeneIDi 855584.
    KEGGi sce:YNL138W.

    Organism-specific databases

    CYGDi YNL138w.
    SGDi S000005082. SRV2.

    Phylogenomic databases

    eggNOGi NOG254262.
    GeneTreei ENSGT00390000017955.
    HOGENOMi HOG000206192.
    KOi K17261.
    OMAi AQGGQTR.
    OrthoDBi EOG7H79BV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33157-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P17555.
    NextBioi 979715.

    Gene expression databases

    Genevestigatori P17555.

    Family and domain databases

    Gene3Di 2.160.20.70. 1 hit.
    InterProi IPR001837. Adenylate_cyclase-assoc_CAP.
    IPR013912. Adenylate_cyclase-assoc_CAP_C.
    IPR013992. Adenylate_cyclase-assoc_CAP_N.
    IPR017901. C-CAP_CF_C-like.
    IPR016098. CAP/MinC_C.
    IPR028417. CAP_CS_C.
    IPR018106. CAP_CS_N.
    IPR028419. CAP_fungal/plant.
    IPR006599. CARP_motif.
    [Graphical view ]
    PANTHERi PTHR10652. PTHR10652. 1 hit.
    PTHR10652:SF0. PTHR10652:SF0. 1 hit.
    Pfami PF08603. CAP_C. 1 hit.
    PF01213. CAP_N. 1 hit.
    [Graphical view ]
    SMARTi SM00673. CARP. 2 hits.
    [Graphical view ]
    SUPFAMi SSF101278. SSF101278. 2 hits.
    SSF69340. SSF69340. 1 hit.
    PROSITEi PS51329. C_CAP_COFACTOR_C. 1 hit.
    PS01088. CAP_1. 1 hit.
    PS01089. CAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein."
      Field J., Vojtek A., Ballester R., Bolger G., Colicelli J., Ferguson K., Gerst J., Kataoka T., Michaeli T., Powers S., Riggs M., Rodgers L., Wieland I., Wheland B., Wigler M.
      Cell 61:319-327(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "SRV2, a gene required for RAS activation of adenylate cyclase in yeast."
      Fedor-Chaiken M., Deschenes R.J., Broach J.R.
      Cell 61:329-340(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
      Mallet L., Bussereau F., Jacquet M.
      Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "A conserved proline-rich region of the Saccharomyces cerevisiae cyclase-associated protein binds SH3 domains and modulates cytoskeletal localization."
      Freeman N.L., Lila T., Mintzer K.A., Chen Z., Pahk A.J., Ren R., Drubin D.G., Field J.
      Mol. Cell. Biol. 16:548-556(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ABP1, SUBCELLULAR LOCATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-454, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 368-526.

    Entry informationi

    Entry nameiCAP_YEAST
    AccessioniPrimary (citable) accession number: P17555
    Secondary accession number(s): D6W144
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8760 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3