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P17555

- CAP_YEAST

UniProt

P17555 - CAP_YEAST

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Protein
Adenylyl cyclase-associated protein
Gene
SRV2, CAP1, YNL138W, N1210, N1838
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The N-terminal domain binds to adenylyl cyclase, thereby enabling adenylyl cyclase to be activated by upstream regulatory signals, such as Ras. The C-terminal domain is required for normal cellular morphology and growth control.

GO - Molecular functioni

  1. actin binding Source: SGD
  2. adenylate cyclase binding Source: SGD
  3. identical protein binding Source: IntAct
  4. protein binding Source: IntAct

GO - Biological processi

  1. Ras protein signal transduction Source: SGD
  2. actin filament organization Source: SGD
  3. actin filament severing Source: SGD
  4. cell morphogenesis Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33157-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyl cyclase-associated protein
Short name:
CAP
Gene namesi
Name:SRV2
Synonyms:CAP1
Ordered Locus Names:YNL138W
ORF Names:N1210, N1838
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL138w.
SGDiS000005082. SRV2.

Subcellular locationi

Cytoplasmcytoskeletonactin patch
Note: Cortical actin patches.1 Publication

GO - Cellular componenti

  1. actin cortical patch Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Adenylyl cyclase-associated protein
PRO_0000205706Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei454 – 4541Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP17555.
PaxDbiP17555.

Expressioni

Gene expression databases

GenevestigatoriP17555.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-4024,EBI-4024
ABP1P158919EBI-4024,EBI-2036
ACT1P600105EBI-4024,EBI-2169
RPL3P141263EBI-4024,EBI-15364
TEF2P029943EBI-4024,EBI-6314

Protein-protein interaction databases

BioGridi35688. 67 interactions.
DIPiDIP-77N.
IntActiP17555. 37 interactions.
MINTiMINT-582270.
STRINGi4932.YNL138W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi371 – 3755
Beta strandi378 – 3825
Beta strandi391 – 3944
Beta strandi400 – 41516
Beta strandi417 – 43317
Beta strandi435 – 4373
Beta strandi439 – 45315
Beta strandi456 – 4627
Beta strandi464 – 4696
Turni473 – 4764
Beta strandi478 – 4836
Beta strandi485 – 4928
Helixi495 – 4973
Beta strandi500 – 5034
Beta strandi508 – 5136
Beta strandi516 – 5216

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K4ZX-ray2.30A/B369-526[»]
1KQ5X-ray3.00A/B369-526[»]
ProteinModelPortaliP17555.
SMRiP17555. Positions 78-252, 369-524.

Miscellaneous databases

EvolutionaryTraceiP17555.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini369 – 504136C-CAP/cofactor C-like
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 168168Adenyl cyclase-binding
Add
BLAST
Regioni354 – 3618Interaction with SH3 domain of ABP1
Regioni370 – 526157Dimerization and actin-binding
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi169 – 369201SH3-binding
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi262 – 30039Ala/Pro/Ser-rich
Add
BLAST
Compositional biasi277 – 2826Poly-Pro

Sequence similaritiesi

Belongs to the CAP family.

Phylogenomic databases

eggNOGiNOG254262.
GeneTreeiENSGT00390000017955.
HOGENOMiHOG000206192.
KOiK17261.
OMAiAQGGQTR.
OrthoDBiEOG7H79BV.

Family and domain databases

Gene3Di2.160.20.70. 1 hit.
InterProiIPR001837. Adenylate_cyclase-assoc_CAP.
IPR013912. Adenylate_cyclase-assoc_CAP_C.
IPR013992. Adenylate_cyclase-assoc_CAP_N.
IPR017901. C-CAP_CF_C-like.
IPR016098. CAP/MinC_C.
IPR028417. CAP_CS_C.
IPR018106. CAP_CS_N.
IPR028419. CAP_fungal/plant.
IPR006599. CARP_motif.
[Graphical view]
PANTHERiPTHR10652. PTHR10652. 1 hit.
PTHR10652:SF0. PTHR10652:SF0. 1 hit.
PfamiPF08603. CAP_C. 1 hit.
PF01213. CAP_N. 1 hit.
[Graphical view]
SMARTiSM00673. CARP. 2 hits.
[Graphical view]
SUPFAMiSSF101278. SSF101278. 2 hits.
SSF69340. SSF69340. 1 hit.
PROSITEiPS51329. C_CAP_COFACTOR_C. 1 hit.
PS01088. CAP_1. 1 hit.
PS01089. CAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17555-1 [UniParc]FASTAAdd to Basket

« Hide

MPDSKYTMQG YNLVKLLKRL EEATARLEDV TIYQEGYIQN KLEASKNNKP    50
SDSGADANTT NEPSAENAPE VEQDPKCITA FQSYIGENID PLVELSGKID 100
TVVLDALQLL KGGFQSQLTF LRAAVRSRKP DYSSQTFADS LRPINENIIK 150
LGQLKESNRQ SKYFAYLSAL SEGAPLFSWV AVDTPVSMVT DFKDAAQFWT 200
NRILKEYRES DPNAVEWVKK FLASFDNLKA YIKEYHTTGV SWKKDGMDFA 250
DAMAQSTKNT GATSSPSPAS ATAAPAPPPP PPAPPASVFE ISNDTPATSS 300
DANKGGIGAV FAELNQGENI TKGLKKVDKS QQTHKNPELR QSSTVSSTGS 350
KSGPPPRPKK PSTLKTKRPP RKELVGNKWF IENYENETES LVIDANKDES 400
IFIGKCSQVL VQIKGKVNAI SLSETESCSV VLDSSISGMD VIKSNKFGIQ 450
VNHSLPQISI DKSDGGNIYL SKESLNTEIY TSCSTAINVN LPIGEDDDYV 500
EFPIPEQMKH SFADGKFKSA VFEHAG 526
Length:526
Mass (Da):57,521
Last modified:August 1, 1990 - v1
Checksum:i0EB4D41205E2D464
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58284 mRNA. Translation: AAA63569.1.
M32663 Genomic DNA. Translation: AAA35094.1.
Z46843 Genomic DNA. Translation: CAA86887.1.
Z71414 Genomic DNA. Translation: CAA96020.1.
BK006947 Genomic DNA. Translation: DAA10410.1.
PIRiA34896.
RefSeqiNP_014261.1. NM_001182976.1.

Genome annotation databases

EnsemblFungiiYNL138W; YNL138W; YNL138W.
GeneIDi855584.
KEGGisce:YNL138W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58284 mRNA. Translation: AAA63569.1 .
M32663 Genomic DNA. Translation: AAA35094.1 .
Z46843 Genomic DNA. Translation: CAA86887.1 .
Z71414 Genomic DNA. Translation: CAA96020.1 .
BK006947 Genomic DNA. Translation: DAA10410.1 .
PIRi A34896.
RefSeqi NP_014261.1. NM_001182976.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K4Z X-ray 2.30 A/B 369-526 [» ]
1KQ5 X-ray 3.00 A/B 369-526 [» ]
ProteinModelPortali P17555.
SMRi P17555. Positions 78-252, 369-524.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35688. 67 interactions.
DIPi DIP-77N.
IntActi P17555. 37 interactions.
MINTi MINT-582270.
STRINGi 4932.YNL138W.

Proteomic databases

MaxQBi P17555.
PaxDbi P17555.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL138W ; YNL138W ; YNL138W .
GeneIDi 855584.
KEGGi sce:YNL138W.

Organism-specific databases

CYGDi YNL138w.
SGDi S000005082. SRV2.

Phylogenomic databases

eggNOGi NOG254262.
GeneTreei ENSGT00390000017955.
HOGENOMi HOG000206192.
KOi K17261.
OMAi AQGGQTR.
OrthoDBi EOG7H79BV.

Enzyme and pathway databases

BioCyci YEAST:G3O-33157-MONOMER.

Miscellaneous databases

EvolutionaryTracei P17555.
NextBioi 979715.

Gene expression databases

Genevestigatori P17555.

Family and domain databases

Gene3Di 2.160.20.70. 1 hit.
InterProi IPR001837. Adenylate_cyclase-assoc_CAP.
IPR013912. Adenylate_cyclase-assoc_CAP_C.
IPR013992. Adenylate_cyclase-assoc_CAP_N.
IPR017901. C-CAP_CF_C-like.
IPR016098. CAP/MinC_C.
IPR028417. CAP_CS_C.
IPR018106. CAP_CS_N.
IPR028419. CAP_fungal/plant.
IPR006599. CARP_motif.
[Graphical view ]
PANTHERi PTHR10652. PTHR10652. 1 hit.
PTHR10652:SF0. PTHR10652:SF0. 1 hit.
Pfami PF08603. CAP_C. 1 hit.
PF01213. CAP_N. 1 hit.
[Graphical view ]
SMARTi SM00673. CARP. 2 hits.
[Graphical view ]
SUPFAMi SSF101278. SSF101278. 2 hits.
SSF69340. SSF69340. 1 hit.
PROSITEi PS51329. C_CAP_COFACTOR_C. 1 hit.
PS01088. CAP_1. 1 hit.
PS01089. CAP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein."
    Field J., Vojtek A., Ballester R., Bolger G., Colicelli J., Ferguson K., Gerst J., Kataoka T., Michaeli T., Powers S., Riggs M., Rodgers L., Wieland I., Wheland B., Wigler M.
    Cell 61:319-327(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "SRV2, a gene required for RAS activation of adenylate cyclase in yeast."
    Fedor-Chaiken M., Deschenes R.J., Broach J.R.
    Cell 61:329-340(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames."
    Mallet L., Bussereau F., Jacquet M.
    Yeast 11:1195-1209(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "A conserved proline-rich region of the Saccharomyces cerevisiae cyclase-associated protein binds SH3 domains and modulates cytoskeletal localization."
    Freeman N.L., Lila T., Mintzer K.A., Chen Z., Pahk A.J., Ren R., Drubin D.G., Field J.
    Mol. Cell. Biol. 16:548-556(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ABP1, SUBCELLULAR LOCATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-454, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 368-526.

Entry informationi

Entry nameiCAP_YEAST
AccessioniPrimary (citable) accession number: P17555
Secondary accession number(s): D6W144
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 11, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8760 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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