P17554 (LUCI_VARHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Luciferin 2-monooxygenase EC=1.13.12.6 Alternative name(s): Cypridina-type luciferase
Organism	Vargula hilgendorfii (Sea firefly) (Cypridina hilgendorfii)
Taxonomic identifier	6674 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Crustacea › Ostracoda › Myodocopa › Myodocopida › Cypridinoidea › Cypridinidae › Vargula

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Cypridina luciferin + O2 = oxidized Cypridina luciferin + CO2 + light.
Post-translational modification	The cysteine residues presumably exist in intramolecular disulfide bridges. The N-terminus is blocked.
Miscellaneous	60 kcal/mol are required for the blue emission of light (460 nm) due to the oxidation of luciferin via a dioxetanone intermediate, in which the excited state oxyluciferin bound to luciferase is the emitter.
Sequence similarities	Contains 2 VWFD domains.

Ontologies

Keywords
Biological process	Luminescence
Domain	Repeat Signal
Molecular function	Decarboxylase Lyase Monooxygenase Oxidoreductase Photoprotein
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Cypridina-luciferin 2-monooxygenase activity Inferred from electronic annotation. Source: EC carboxy-lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 11	11	Potential
	Chain	12 – 555	544	Luciferin 2-monooxygenase		PRO_0000021626
Regions
	Domain	81 – 297	217	VWFD 1
	Domain	320 – 525	206	VWFD 2
	Compositional bias	39 – 82	44	Cys-rich
Amino acid modifications
	Glycosylation	186	1	N-linked (GlcNAc...) Potential
	Glycosylation	408	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P17554 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 2FB150371A80A0DF	FASTA	555	61,627
	10 20 30 40 50 60 MKIIILSVIL AYCVTDNCQD ACPVEAEPPS STPTVPTSCE AKEGECIDTR CATCKRDILS 70 80 90 100 110 120 DGLCENKPGK TCCRMCQYVI ECRVEAAGYF RTFYGKRFNF QEPGKYVLAR GTKGGDWSVT 130 140 150 160 170 180 LTMENLDGQK GAVLTKTTLE VAGDVIDITQ ATADPITVNG GADPVIANPF TIGEVTIAVV 190 200 210 220 230 240 EIPGFNITVI EFFKLIVIDI LGGRSVRIAP DTANKGLISG ICGNLEMNDA DDFTTDADQL 250 260 270 280 290 300 AIQPNINKEF DGCPFYGNPS DIEYCKGLME PYRAVCRNNI NFYYYTLSCA FAYCMGGEER 310 320 330 340 350 360 AKHVLFDYVE TCAAPETRGT CVLSGHTFYD TFDKARYQFQ GPCKEILMAA DCYWNTWDVK 370 380 390 400 410 420 VSHRDVESYT EVEKVTIRKQ STVVDLIVDG KQVKVGGVDV SIPYSSENTS IYWQDGDILT 430 440 450 460 470 480 TAILPEALVV KFNFKQLLVV HIRDPFDGKT CGICGNYNQD STDDFFDAEG ACALTPNPPG 490 500 510 520 530 540 CTEEQKPEAE RLCNNLFDSS IDEKCNVCYK PDRIARCMYE YCLRGQQGFC DHAWEFKKEC 550 YIKHGDTLEV PPECQ

References

[1]	"Cloning and expression of cDNA for the luciferase from the marine ostracod Vargula hilgendorfii." Thompson E.M., Nagata S., Tsuji F.I. Proc. Natl. Acad. Sci. U.S.A. 86:6567-6571(1989) [PubMed: 2771943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M25666 mRNA. Translation: AAA30332.1.
PIR	A33723.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	1.13.12.6. 6599.
Family and domain databases
InterPro	IPR001846. VWF_type-D. [Graphical view]
Pfam	PF00094. VWD. 2 hits. [Graphical view]
SMART	SM00216. VWD. 2 hits. [Graphical view]
PROSITE	PS51233. VWFD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUCI_VARHI

Accession

Primary (citable) accession number: P17554

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 28, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)