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Protein

Proto-oncogene Wnt-3

Gene

Wnt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

GO - Molecular functioni

  • frizzled binding Source: MGI
  • protein domain specific binding Source: UniProtKB
  • receptor agonist activity Source: MGI
  • receptor binding Source: MGI

GO - Biological processi

  • anatomical structure formation involved in morphogenesis Source: MGI
  • anterior/posterior axis specification Source: MGI
  • anterior/posterior pattern specification Source: MGI
  • axon guidance Source: MGI
  • canonical Wnt signaling pathway Source: MGI
  • canonical Wnt signaling pathway involved in mesenchymal stem cell differentiation Source: MGI
  • canonical Wnt signaling pathway involved in osteoblast differentiation Source: MGI
  • cell-cell signaling Source: MGI
  • cell fate commitment Source: GO_Central
  • cell morphogenesis Source: MGI
  • dorsal/ventral axis specification Source: MGI
  • embryonic forelimb morphogenesis Source: MGI
  • embryonic hindlimb morphogenesis Source: MGI
  • gamete generation Source: MGI
  • head morphogenesis Source: MGI
  • limb bud formation Source: MGI
  • limb development Source: MGI
  • mammary gland epithelium development Source: Ensembl
  • mesoderm formation Source: MGI
  • negative regulation of axon extension involved in axon guidance Source: MGI
  • organ morphogenesis Source: MGI
  • positive regulation of collateral sprouting in absence of injury Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of Wnt signaling pathway Source: CACAO
  • signal transduction Source: MGI
  • Spemann organizer formation at the anterior end of the primitive streak Source: MGI
  • Wnt signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-3238698. WNT ligand biogenesis and trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Proto-oncogene Wnt-3
Alternative name(s):
Proto-oncogene Int-4
Gene namesi
Name:Wnt3
Synonyms:Int-4, Wnt-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98955. Wnt3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 355334Proto-oncogene Wnt-3PRO_0000041417Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi80 ↔ 91By similarity
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence analysis
Disulfide bondi131 ↔ 139By similarity
Disulfide bondi141 ↔ 158By similarity
Disulfide bondi206 ↔ 220By similarity
Disulfide bondi208 ↔ 215By similarity
Lipidationi212 – 2121O-palmitoleyl serine; by PORCNBy similarity
Disulfide bondi300 ↔ 315By similarity
Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence analysis
Disulfide bondi330 ↔ 345By similarity
Disulfide bondi332 ↔ 342By similarity
Disulfide bondi337 ↔ 338By similarity

Post-translational modificationi

Palmitoleylation is required for efficient binding to frizzled receptors. Depalmitoleylation leads to Wnt signaling pathway inhibition.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiP17553.
PRIDEiP17553.

PTM databases

PhosphoSiteiP17553.

Expressioni

Tissue specificityi

Dorsal portion of the neural tube, dorsal ectoderm, the branchial arches, and the limb buds.

Gene expression databases

BgeeiP17553.
CleanExiMM_WNT3.
ExpressionAtlasiP17553. baseline and differential.
GenevisibleiP17553. MM.

Interactioni

Subunit structurei

Interacts with PORCN. Interacts with WLS.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KlO350824EBI-1570853,EBI-1570828

GO - Molecular functioni

  • frizzled binding Source: MGI
  • protein domain specific binding Source: UniProtKB
  • receptor agonist activity Source: MGI
  • receptor binding Source: MGI

Protein-protein interaction databases

BioGridi204574. 3 interactions.
DIPiDIP-39897N.
IntActiP17553. 1 interaction.
STRINGi10090.ENSMUSP00000000127.

Structurei

3D structure databases

ProteinModelPortaliP17553.
SMRiP17553. Positions 70-284.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913. Eukaryota.
ENOG410XQZ1. LUCA.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP17553.
KOiK00312.
OMAiMCGCDSH.
OrthoDBiEOG7C8GJ8.
PhylomeDBiP17553.
TreeFamiTF105310.

Family and domain databases

InterProiIPR005817. Wnt.
IPR009141. Wnt3.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01843. WNT3PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17553-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPHLLGLLL GLLLSGTRVL AGYPIWWSLA LGQQYTSLAS QPLLCGSIPG
60 70 80 90 100
LVPKQLRFCR NYIEIMPSVA EGVKLGIQEC QHQFRGRRWN CTTIDDSLAI
110 120 130 140 150
FGPVLDKATR ESAFVHAIAS AGVAFAVTRS CAEGTSTICG CDSHHKGPPG
160 170 180 190 200
EGWKWGGCSE DADFGVLVSR EFADARENRP DARSAMNKHN NEAGRTTILD
210 220 230 240 250
HMHLKCKCHG LSGSCEVKTC WWAQPDFRAI GDFLKDKYDS ASEMVVEKHR
260 270 280 290 300
ESRGWVETLR AKYALFKPPT ERDLVYYENS PNFCEPNPET GSFGTRDRTC
310 320 330 340 350
NVTSHGIDGC DLLCCGRGHN TRTEKRKEKC HCVFHWCCYV SCQECIRIYD

VHTCK
Length:355
Mass (Da):39,659
Last modified:August 1, 1990 - v1
Checksum:iF31CFD65E43E9C17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32502 mRNA. Translation: AAB38109.1.
CCDSiCCDS25523.1.
PIRiA35503.
RefSeqiNP_033547.1. NM_009521.2.
UniGeneiMm.159091.

Genome annotation databases

EnsembliENSMUST00000000127; ENSMUSP00000000127; ENSMUSG00000000125.
GeneIDi22415.
KEGGimmu:22415.
UCSCiuc007lvs.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32502 mRNA. Translation: AAB38109.1.
CCDSiCCDS25523.1.
PIRiA35503.
RefSeqiNP_033547.1. NM_009521.2.
UniGeneiMm.159091.

3D structure databases

ProteinModelPortaliP17553.
SMRiP17553. Positions 70-284.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204574. 3 interactions.
DIPiDIP-39897N.
IntActiP17553. 1 interaction.
STRINGi10090.ENSMUSP00000000127.

PTM databases

PhosphoSiteiP17553.

Proteomic databases

PaxDbiP17553.
PRIDEiP17553.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000127; ENSMUSP00000000127; ENSMUSG00000000125.
GeneIDi22415.
KEGGimmu:22415.
UCSCiuc007lvs.2. mouse.

Organism-specific databases

CTDi7473.
MGIiMGI:98955. Wnt3.

Phylogenomic databases

eggNOGiKOG3913. Eukaryota.
ENOG410XQZ1. LUCA.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP17553.
KOiK00312.
OMAiMCGCDSH.
OrthoDBiEOG7C8GJ8.
PhylomeDBiP17553.
TreeFamiTF105310.

Enzyme and pathway databases

ReactomeiR-MMU-3238698. WNT ligand biogenesis and trafficking.

Miscellaneous databases

PROiP17553.
SOURCEiSearch...

Gene expression databases

BgeeiP17553.
CleanExiMM_WNT3.
ExpressionAtlasiP17553. baseline and differential.
GenevisibleiP17553. MM.

Family and domain databases

InterProiIPR005817. Wnt.
IPR009141. Wnt3.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01843. WNT3PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Wnt-3, a gene activated by proviral insertion in mouse mammary tumors, is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and adult brain."
    Roelink H., Wagenaar E., Lopes da Silva S., Nusse R.
    Proc. Natl. Acad. Sci. U.S.A. 87:4519-4523(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Embryo.
  2. "The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family."
    Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.
    Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PORCN.
  3. "Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation."
    Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W.
    Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WLS.

Entry informationi

Entry nameiWNT3_MOUSE
AccessioniPrimary (citable) accession number: P17553
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 6, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Some mouse mammary tumors induced by mouse mammary tumor virus (MMTV) contain a provirus integrated into a host cell region which has been named Wnt3.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.