P17553 (WNT3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proto-oncogene Wnt-3

Alternative name(s):
Proto-oncogene Int-4

Gene names

Name:	Wnt3
Synonyms:	Int-4, Wnt-3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Subunit structure	Interacts with PORCN. Interacts with WLS. Ref.2 Ref.3
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Dorsal portion of the neural tube, dorsal ectoderm, the branchial arches, and the limb buds.
Involvement in disease	Note=Some mouse mammary tumors induced by mouse mammary tumor virus (MMTV) contain a provirus integrated into a host cell region which has been named Wnt3.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Disease	Proto-oncogene
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Spemann organizer formation at the anterior end of the primitive streak Inferred from mutant phenotype. Source: MGI Wnt receptor signaling pathway, calcium modulating pathway Inferred from Biological aspect of Ancestor. Source: RefGenome anterior/posterior axis specification Inferred from mutant phenotype. Source: MGI axon guidance Inferred from direct assay. Source: MGI dorsal/ventral axis specification Inferred from mutant phenotype. Source: MGI embryonic forelimb morphogenesis Inferred from mutant phenotype. Source: MGI embryonic hindlimb morphogenesis Inferred from mutant phenotype. Source: MGI embryonic pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome head morphogenesis Inferred from genetic interaction. Source: MGI hemopoietic stem cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome inner ear morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome mesoderm formation Inferred from mutant phenotype. Source: MGI midbrain-hindbrain boundary development Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of axon extension involved in axon guidance Inferred from direct assay. Source: MGI negative regulation of fat cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of collateral sprouting in absence of injury Inferred from direct assay. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular component	early endosome Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome late endosome Inferred from Biological aspect of Ancestor. Source: RefGenome membrane raft Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	frizzled-2 binding Inferred from Biological aspect of Ancestor. Source: RefGenome protein domain specific binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Kl	O35082	4	EBI-1570853,EBI-1570828

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 355	334	Proto-oncogene Wnt-3		PRO_0000041417
Amino acid modifications
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	301	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P17553 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: F31CFD65E43E9C17	FASTA	355	39,659
	10 20 30 40 50 60 MEPHLLGLLL GLLLSGTRVL AGYPIWWSLA LGQQYTSLAS QPLLCGSIPG LVPKQLRFCR 70 80 90 100 110 120 NYIEIMPSVA EGVKLGIQEC QHQFRGRRWN CTTIDDSLAI FGPVLDKATR ESAFVHAIAS 130 140 150 160 170 180 AGVAFAVTRS CAEGTSTICG CDSHHKGPPG EGWKWGGCSE DADFGVLVSR EFADARENRP 190 200 210 220 230 240 DARSAMNKHN NEAGRTTILD HMHLKCKCHG LSGSCEVKTC WWAQPDFRAI GDFLKDKYDS 250 260 270 280 290 300 ASEMVVEKHR ESRGWVETLR AKYALFKPPT ERDLVYYENS PNFCEPNPET GSFGTRDRTC 310 320 330 340 350 NVTSHGIDGC DLLCCGRGHN TRTEKRKEKC HCVFHWCCYV SCQECIRIYD VHTCK

References

[1]	"Wnt-3, a gene activated by proviral insertion in mouse mammary tumors, is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and adult brain." Roelink H., Wagenaar E., Lopes da Silva S., Nusse R. Proc. Natl. Acad. Sci. U.S.A. 87:4519-4523(1990) [PubMed: 2162045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Embryo.
[2]	"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family." Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T. Eur. J. Biochem. 267:4300-4311(2000) [PubMed: 10866835] [Abstract] Cited for: INTERACTION WITH PORCN.
[3]	"Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation." Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W. Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed: 19841259] [Abstract] Cited for: INTERACTION WITH WLS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M32502 mRNA. Translation: AAB38109.1.
IPI	IPI00110016.
PIR	A35503.
RefSeq	NP_033547.1. NM_009521.2.
UniGene	Mm.159091.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-39897N.
IntAct	P17553. 1 interaction.
STRING	P17553.
Proteomic databases
PRIDE	P17553.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000000127; ENSMUSP00000000127; ENSMUSG00000000125.
GeneID	22415.
KEGG	mmu:22415.
Organism-specific databases
CTD	7473.
MGI	MGI:98955. Wnt3.
Phylogenomic databases
HOGENOM	HBG446188.
HOVERGEN	HBG001595.
InParanoid	P17553.
OMA	MCGCDSH.
OrthoDB	EOG4XD3R9.
PhylomeDB	P17553.
Gene expression databases
ArrayExpress	P17553.
Bgee	P17553.
CleanEx	MM_WNT3.
Genevestigator	P17553.
GermOnline	ENSMUSG00000000125. Mus musculus.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009141. Wnt3. IPR018161. Wnt_grthfactor_CS. [Graphical view]
KO	K00312.
PANTHER	PTHR12027. Wnt. 1 hit. PTHR12027:SF18. Wnt3. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01843. WNT3PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302829.
SOURCE	Search...

Entry information

Entry name

WNT3_MOUSE

Accession

Primary (citable) accession number: P17553

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	November 16, 2011
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program