Reviewed, UniProtKB/Swiss-Prot P17549 (CP53_ASPNG)

Names and origin

Protein names

Recommended name:
    Benzoate 4-monooxygenase
    EC=1.14.13.12
Alternative name(s):
    Benzoate-para-hydroxylase
      Short name=BpH
    Cytochrome P450 53

Gene names

Name:	bphA
Synonyms:	cyp53, cyp53A1

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for cytochrome P450 dependent benzoate hydroxylation in microsomes; requires cprA as the mediator in electron donation from NADPH.
Catalytic activity	Benzoate + NADPH + O2 = 4-hydroxybenzoate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Ref.3 Inferred from direct assay. Source: UniProtKB
Cellular component	microsome Ref.3 Inferred from direct assay. Source: UniProtKB
Molecular function	benzoate 4-monooxygenase activity Ref.3 Inferred from direct assay. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Benzoate 4-monooxygenase		PRO_0000052038
Sites
	Metal binding	461	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17549-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 63FFBFDF703EC30B	FASTA	517	58,016
	10 20 30 40 50 60 MLALLLSPYG AYLGLALLVL YYLLPYLKRA HLRDIPAPGL AAFTNFWLLL QTRRGHRFVV 70 80 90 100 110 120 VDNAHKKYGK LVRIAPRHTS IADDGAIQAV YGHGNGFLKS DFYDAFVSIH RGLFNTRDRA 130 140 150 160 170 180 EHTRKRKTVS HTFSMKSIGQ FEQYIHGNIE LFVKQWNRMA DTQRNPKTGF ASLDALNWFN 190 200 210 220 230 240 YLAFDIIGDL AFGAPFGMLD KGKDFAEMRK TPDSPPSYVQ AVEVLNRRGE VSATLGCYPA 250 260 270 280 290 300 LKPFAKYLPD SFFRDGIQAV EDLAGIAVAR VNERLRPEVM ANNTRVDLLA RLMEGKDSNG 310 320 330 340 350 360 EKLGRAELTA EALTQLIAGS DTTSNTSCAI LYWCMRTPGV IEKLHKALDE AIPQDVDVPT 370 380 390 400 410 420 HAMVKDIPYL QWVIWETMRI HSTSAMGLPR EIPAGNPPVT ISGHTFYPGD VVSVPSYTIH 430 440 450 460 470 480 RSKEIWGPDA EQFVPERWDP ARLTPRQKAA FIPFSTGPRA CVGRNVAEME LLVICGTVFR 490 500 510 LFEFEMQQEG PMETREGFLR KPLGLQVGMK RRQPGSA

References

[1]	"Isolation and molecular characterisation of the benzoate-para-hydroxylase gene (bphA) of Aspergillus niger: a member of a new gene family of the cytochrome P450 superfamily." van Gorcom R.F.M., Boschloo J.G., Kuijvenhoven A., Lange J., van Vark A.J., Bos C.J., van Balken J.A.M., Pouwels P.H., van den Hondel C.A.M.J.J. Mol. Gen. Genet. 223:192-197(1990) [PubMed: 2250647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 1055 / DSM 2061.
[2]	"Yeast heterologous expression of a benzoate 4-hydroxylase (CYP53) from Aspergillus niger." Higgins U.M., Darby R.M., Maddison A., Lamb D.C., Draper J. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger." Faber B.W., van Gorcom R.F.M., Duine J.A. Arch. Biochem. Biophys. 394:245-254(2001) [PubMed: 11594739] [Abstract] Cited for: CHARACTERIZATION. Strain: T18.5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52521 Genomic DNA. Translation: CAA36753.1. AJ347748 mRNA. Translation: CAC69995.1.
PIR	S12015.
3D structure databases
HSSP	HSSP built from PDB template 1JPZ based on UniProtKB P14779.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.12. 277.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP53_ASPNG

Accession

Primary (citable) accession number: P17549

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	March 24, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)