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P17542 (TAL1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 154. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-cell acute lymphocytic leukemia protein 1

Short name=TAL-1
Alternative name(s):
Class A basic helix-loop-helix protein 17
Short name=bHLHa17
Stem cell protein
T-cell leukemia/lymphoma protein 5
Gene names
Name:TAL1
Synonyms:BHLHA17, SCL, TCL5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation By similarity. Ref.7

Subunit structure

Efficient DNA binding requires dimerization with another bHLH protein. Forms heterodimers with TCF3. Binds to the LIM domain containing protein LMO2 and to DRG1. Can assemble in a complex with LDB1 and LMO2. Component of a TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3 By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

Leukemic stem cell.

Domain

The helix-loop-helix domain is necessary and sufficient for the interaction with DRG1.

Post-translational modification

Phosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia By similarity. Ref.8

Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells By similarity.

Involvement in disease

A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). Translocation t(1;14)(p32;q11) with T-cell receptor alpha chain (TCRA) genes.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processDifferentiation
Transcription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Chromosomal rearrangement
   DiseaseProto-oncogene
   LigandDNA-binding
   Molecular functionDevelopmental protein
   PTMPhosphoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processangiogenesis

Inferred from electronic annotation. Source: Ensembl

astrocyte fate commitment

Inferred from electronic annotation. Source: Ensembl

basophil differentiation

Inferred from expression pattern PubMed 7678994. Source: BHF-UCL

cell fate commitment

Inferred from sequence or structural similarity. Source: BHF-UCL

definitive hemopoiesis

Inferred from electronic annotation. Source: Ensembl

embryonic hemopoiesis

Inferred from sequence or structural similarity. Source: UniProtKB

erythrocyte differentiation

Inferred from mutant phenotype Ref.7. Source: BHF-UCL

erythrocyte maturation

Inferred from electronic annotation. Source: Ensembl

hemangioblast cell differentiation

Inferred from electronic annotation. Source: Ensembl

hematopoietic stem cell differentiation

Inferred from electronic annotation. Source: Ensembl

hemopoiesis

Inferred from sequence or structural similarity. Source: BHF-UCL

locomotory behavior

Inferred from electronic annotation. Source: Ensembl

megakaryocyte development

Inferred from electronic annotation. Source: Ensembl

megakaryocyte differentiation

Inferred from expression pattern PubMed 7678994. Source: BHF-UCL

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

platelet formation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell division

Inferred from mutant phenotype PubMed 20855495. Source: BHF-UCL

positive regulation of chromatin assembly or disassembly

Inferred from mutant phenotype PubMed 20855495. Source: BHF-UCL

positive regulation of erythrocyte differentiation

Inferred from direct assay PubMed 20028976. Source: UniProtKB

positive regulation of mitotic cell cycle

Inferred from mutant phenotype PubMed 20855495. Source: BHF-UCL

positive regulation of protein complex assembly

Inferred from direct assay PubMed 20855495. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 20855495. Source: BHF-UCL

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 20028976. Source: UniProtKB

regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

regulation of mast cell differentiation

Inferred from electronic annotation. Source: Ensembl

regulation of stem cell maintenance

Inferred from electronic annotation. Source: Ensembl

regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: BHF-UCL

spinal cord association neuron differentiation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentnuclear chromatin

Inferred from direct assay PubMed 20855495. Source: BHF-UCL

nucleus

Inferred from direct assay PubMed 19497860. Source: BHF-UCL

transcription factor complex

Inferred from direct assay PubMed 15920471. Source: BHF-UCL

   Molecular_functionE-box binding

Inferred from direct assay PubMed 20855495. Source: BHF-UCL

RNA polymerase II distal enhancer sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

RNA polymerase II transcription factor binding

Inferred from physical interaction PubMed 15920471. Source: BHF-UCL

chromatin binding

Inferred from direct assay PubMed 16763211. Source: MGI

enzyme binding

Inferred from physical interaction PubMed 19497860. Source: BHF-UCL

histone deacetylase binding

Inferred from physical interaction PubMed 19497860. Source: BHF-UCL

protein binding

Inferred from physical interaction PubMed 20028976. Source: UniProtKB

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 19497860. Source: BHF-UCL

transcription regulatory region DNA binding

Inferred from sequence or structural similarity. Source: BHF-UCL

Complete GO annotation...

Binary interactions

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: The splicing pattern is cell-lineage dependent.
Isoform PP42-TAL1 (identifier: P17542-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform PP39-TAL1 (identifier: P17542-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.
Isoform PP22-TAL1 (identifier: P17542-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-175: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331T-cell acute lymphocytic leukemia protein 1
PRO_0000127454

Regions

Domain187 – 23953bHLH
Compositional bias89 – 13244Pro-rich
Compositional bias263 – 27412Poly-Gly

Amino acid modifications

Modified residue1221Phosphoserine Ref.9

Natural variations

Alternative sequence1 – 175175Missing in isoform PP22-TAL1.
VSP_002154
Alternative sequence1 – 2525Missing in isoform PP39-TAL1.
VSP_002153

Secondary structure

..... 331
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform PP42-TAL1 [UniParc].

Last modified November 1, 1991. Version 2.
Checksum: 33BBE31589DBB7C7

FASTA33134,271
        10         20         30         40         50         60 
MTERPPSEAA RSDPQLEGRD AAEASMAPPH LVLLNGVAKE TSRAAAAEPP VIELGARGGP 

        70         80         90        100        110        120 
GGGPAGGGGA ARDLKGRDAA TAEARHRVPT TELCRPPGPA PAPAPASVTA ELPGDGRMVQ 

       130        140        150        160        170        180 
LSPPALAAPA APGRALLYSL SQPLASLGSG FFGEPDAFPM FTTNNRVKRR PSPYEMEITD 

       190        200        210        220        230        240 
GPHTKVVRRI FTNSRERWRQ QNVNGAFAEL RKLIPTHPPD KKLSKNEILR LAMKYINFLA 

       250        260        270        280        290        300 
KLLNDQEEEG TQRAKTGKDP VVGAGGGGGG GGGGAPPDDL LQDVLSPNSS CGSSLDGAAS 

       310        320        330 
PDSYTEEPAP KHTARSLHPA MLPAADGAGP R 

« Hide

Isoform PP39-TAL1 [UniParc].

Checksum: 4457C743678771FE
Show »

FASTA30631,617
Isoform PP22-TAL1 [UniParc].

Checksum: 5BC0D5F12E261CDE
Show »

FASTA15616,548

References

« Hide 'large scale' references
[1]"The SCL gene is formed from a transcriptionally complex locus."
Aplan P.D., Begley C.G., Bertness V., Nussmeier M., Ezquerra A., Coligan J., Kirsch I.R.
Mol. Cell. Biol. 10:6426-6435(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Coding sequences of the tal-1 gene are disrupted by chromosome translocation in human T cell leukemia."
Chen Q., Yang C.Y.C., Tsan J.T., Xia Y., Ragab A.H., Peiper S.C., Carroll A., Baer R.
J. Exp. Med. 172:1403-1408(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 106-148.
[5]"The gene SCL is expressed during early hematopoiesis and encodes a differentiation-related DNA-binding motif."
Begley C.G., Aplan P.D., Denning S.M., Haynes B.F., Waldmann T.A., Kirsch I.R.
Proc. Natl. Acad. Sci. U.S.A. 86:10128-10132(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 118-331.
Tissue: Bone marrow.
[6]"The tal gene undergoes chromosome translocation in T cell leukemia and potentially encodes a helix-loop-helix protein."
Chen Q., Cheng J.-T., Tsai L.H., Schneider N., Buchanan G., Carroll A., Crist W., Ozanne B., Siciliano M.J., Baer R.
EMBO J. 9:415-424(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 181-331.
[7]"The SCL gene product: a positive regulator of erythroid differentiation."
Aplan P.D., Nakahara K., Orkin S.H., Kirsch I.R.
EMBO J. 11:4073-4081(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Products of the TAL1 oncogene: basic helix-loop-helix proteins phosphorylated at serine residues."
Cheng J.-T., Hsu H.-L., Hwang L.-Y., Baer R.
Oncogene 8:677-683(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION.
[9]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M61108 mRNA. Translation: AAA36600.1.
M61103 Genomic DNA. No translation available.
M61104 Genomic DNA. No translation available.
M61105 Genomic DNA. No translation available.
M63572 Genomic DNA. No translation available.
M63589, M63576, M63584 Genomic DNA. Translation: AAA36599.1.
AL135960 Genomic DNA. Translation: CAB72103.1.
CH471059 Genomic DNA. Translation: EAX06875.1.
CH471059 Genomic DNA. Translation: EAX06876.1.
CH471059 Genomic DNA. Translation: EAX06877.1.
X58621 Genomic DNA. Translation: CAA41476.1. Sequence problems.
X58622 Genomic DNA. Translation: CAA41477.1.
M29038 mRNA. Translation: AAA36598.1.
X51990 mRNA. Translation: CAA36246.1.
CCDSCCDS547.1. [P17542-1]
PIRA36358.
I38253.
RefSeqNP_001274276.1. NM_001287347.2. [P17542-1]
NP_001277332.1. NM_001290403.1. [P17542-1]
NP_001277333.1. NM_001290404.1. [P17542-1]
NP_001277334.1. NM_001290405.1. [P17542-1]
NP_001277335.1. NM_001290406.1.
NP_003180.1. NM_003189.5. [P17542-1]
XP_005271216.1. XM_005271159.2. [P17542-1]
XP_005271217.1. XM_005271160.2. [P17542-1]
XP_006710925.1. XM_006710862.1. [P17542-1]
UniGeneHs.705618.
Hs.737706.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2YPAX-ray2.80A180-253[»]
2YPBX-ray2.87A180-253[»]
ProteinModelPortalP17542.
SMRP17542. Positions 182-248.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112749. 50 interactions.
DIPDIP-40640N.
IntActP17542. 15 interactions.
MINTMINT-2801392.
STRING9606.ENSP00000294339.

PTM databases

PhosphoSiteP17542.

Polymorphism databases

DMDM134305.

Proteomic databases

MaxQBP17542.
PaxDbP17542.
PRIDEP17542.

Protocols and materials databases

DNASU6886.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000294339; ENSP00000294339; ENSG00000162367. [P17542-1]
ENST00000371884; ENSP00000360951; ENSG00000162367. [P17542-1]
GeneID6886.
KEGGhsa:6886.
UCSCuc001cqx.2. human. [P17542-1]

Organism-specific databases

CTD6886.
GeneCardsGC01M047681.
H-InvDBHIX0029300.
HGNCHGNC:11556. TAL1.
HPACAB017805.
MIM187040. gene.
neXtProtNX_P17542.
Orphanet99861. Precursor T-cell acute lymphoblastic leukemia.
PharmGKBPA36326.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG307510.
HOGENOMHOG000113414.
HOVERGENHBG005018.
KOK09068.
OrthoDBEOG7SN8DF.
PhylomeDBP17542.
TreeFamTF315153.

Enzyme and pathway databases

SignaLinkP17542.

Gene expression databases

ArrayExpressP17542.
BgeeP17542.
CleanExHS_TAL1.
GenevestigatorP17542.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTAL1.
GenomeRNAi6886.
NextBio26909.
PMAP-CutDBP17542.
PROP17542.
SOURCESearch...

Entry information

Entry nameTAL1_HUMAN
AccessionPrimary (citable) accession number: P17542
Secondary accession number(s): D3DQ24
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 1, 1991
Last modified: July 9, 2014
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM