Reviewed, UniProtKB/Swiss-Prot P17541 (CHIA_CUCSA)

Names and origin

Protein names	Recommended name:     Acidic endochitinase     EC=3.2.1.14
Organism	Cucumis sativus (Cucumber)
Taxonomic identifier	3659 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucumis

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein functions as a defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted › extracellular space.
Induction	By salicylate and upon tobacco necrosis virus infection.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25
	Chain	26 – 292	267	Acidic endochitinase		PRO_0000011916
Sites
	Active site	152	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	45 ↔ 92		By similarity
	Disulfide bond	75 ↔ 82		By similarity
	Disulfide bond	180 ↔ 209		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17541-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 2DE39D42BBDB0093	FASTA	292	30,774
	10 20 30 40 50 60 MAAHKITTTL SIFFLLSSIF RSSDAAGIAI YWGQNGNEGS LASTCATGNY EFVNIAFLSS 70 80 90 100 110 120 FGSGQAPVLN LAGHCNPDNN GCAFLSDEIN SCKSQNVKVL LSIGGGAGSY SLSSADDAKQ 130 140 150 160 170 180 VANFIWNSYL GGQSDSRPLG AAVLDGVDFD IESGSGQFWD VLAQELKNFG QVILSAAPQC 190 200 210 220 230 240 PIPDAHLDAA IKTGLFDSVW VQFYNNPPCM FADNADNLLS SWNQWTAFPT SKLYMGLPAA 250 260 270 280 290 REAAPSGGFI PADVLISQVL PTIKASSNYG GVMLWSKAFD NGYSDSIKGS IG

References

[1]	"Isolation of a complementary DNA encoding a chitinase with structural homology to a bifunctional lysozyme/chitinase." Metraux J.P., Burkhart W., Moyer M., Dincher S., Middlesteadt W., Williams S., Payne G., Carnes M., Ryals J. Proc. Natl. Acad. Sci. U.S.A. 86:896-900(1989) [PubMed: 2915985] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Wisconsin SMR-58.
[2]	"Regulation of cucumber class III chitinase gene expression." Lawton K., Beck J., Potter S., Ward E., Ryals J. Mol. Plant Microbe Interact. 7:48-57(1994) [PubMed: 8167370] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M24365 mRNA. Translation: AAA33120.1. M84214 Unassigned DNA. Translation: AAC37395.1.
PIR	A31455.
3D structure databases
HSSP	HSSP built from PDB template 2HVM based on UniProtKB P23472.
ModBase	Search...
Protein family/group databases
CAZy	GH18. Glycoside Hydrolase Family 18.
Enzyme and pathway databases
BRENDA	3.2.1.14. 1241.
Family and domain databases
InterPro	IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIA_CUCSA

Accession

Primary (citable) accession number: P17541

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	September 22, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)