Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P17540

- KCRS_HUMAN

UniProt

P17540 - KCRS_HUMAN

Protein

Creatine kinase S-type, mitochondrial

Gene

CKMT2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 148 (01 Oct 2014)
      Sequence version 2 (17 Oct 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

    Catalytic activityi

    ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei225 – 2251ATP
    Binding sitei270 – 2701ATPPROSITE-ProRule annotation
    Binding sitei326 – 3261ATP
    Binding sitei369 – 3691ATP

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi162 – 1665ATP
    Nucleotide bindingi354 – 3596ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. creatine kinase activity Source: ProtInc

    GO - Biological processi

    1. cellular nitrogen compound metabolic process Source: Reactome
    2. creatine metabolic process Source: Reactome
    3. muscle contraction Source: ProtInc
    4. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS05555-MONOMER.
    ReactomeiREACT_813. Creatine metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Creatine kinase S-type, mitochondrial (EC:2.7.3.2)
    Alternative name(s):
    Basic-type mitochondrial creatine kinase
    Short name:
    Mib-CK
    Sarcomeric mitochondrial creatine kinase
    Short name:
    S-MtCK
    Gene namesi
    Name:CKMT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:1996. CKMT2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: Reactome
    2. mitochondrion Source: UniProt

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26534.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3939Mitochondrion1 PublicationAdd
    BLAST
    Chaini40 – 419380Creatine kinase S-type, mitochondrialPRO_0000016594Add
    BLAST

    Proteomic databases

    MaxQBiP17540.
    PaxDbiP17540.
    PRIDEiP17540.

    2D gel databases

    UCD-2DPAGEP17540.

    PTM databases

    PhosphoSiteiP17540.

    Expressioni

    Tissue specificityi

    Sarcomere-specific. Found only in heart and skeletal muscles.

    Gene expression databases

    ArrayExpressiP17540.
    BgeeiP17540.
    CleanExiHS_CKMT2.
    GenevestigatoriP17540.

    Organism-specific databases

    HPAiHPA051880.

    Interactioni

    Subunit structurei

    Exists as an octamer composed of four CKMT2 homodimers.1 Publication

    Protein-protein interaction databases

    BioGridi107580. 8 interactions.
    IntActiP17540. 6 interactions.
    MINTiMINT-5004086.
    STRINGi9606.ENSP00000254035.

    Structurei

    Secondary structure

    1
    419
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi50 – 534
    Helixi63 – 675
    Helixi70 – 767
    Helixi87 – 9610
    Helixi115 – 1184
    Helixi120 – 13011
    Turni131 – 1333
    Turni136 – 1383
    Beta strandi165 – 1695
    Turni177 – 1793
    Helixi182 – 19716
    Helixi201 – 2033
    Beta strandi205 – 2106
    Helixi215 – 2239
    Helixi234 – 2374
    Turni238 – 24811
    Beta strandi250 – 2545
    Beta strandi257 – 27317
    Helixi280 – 30021
    Turni309 – 3113
    Helixi318 – 3203
    Beta strandi326 – 3327
    Helixi334 – 3385
    Helixi342 – 3487
    Beta strandi351 – 3555
    Beta strandi367 – 3737
    Beta strandi376 – 3783
    Helixi380 – 3889

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2GL6X-ray2.30A/B/C/D/E/F/G/H27-416[»]
    ProteinModelPortaliP17540.
    SMRiP17540. Positions 47-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP17540.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini46 – 13287Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini159 – 401243Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni40 – 6425Cardiolipin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG3869.
    HOGENOMiHOG000232165.
    HOVERGENiHBG001339.
    InParanoidiP17540.
    KOiK00933.
    OMAiGRGWEFM.
    OrthoDBiEOG7XM2XW.
    PhylomeDBiP17540.
    TreeFamiTF314214.

    Family and domain databases

    Gene3Di1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view]
    PfamiPF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48034. SSF48034. 1 hit.
    PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P17540-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASIFSKLLT GRNASLLFAT MGTSVLTTGY LLNRQKVCAE VREQPRLFPP    50
    SADYPDLRKH NNCMAECLTP AIYAKLRNKV TPNGYTLDQC IQTGVDNPGH 100
    PFIKTVGMVA GDEESYEVFA DLFDPVIKLR HNGYDPRVMK HTTDLDASKI 150
    TQGQFDEHYV LSSRVRTGRS IRGLSLPPAC TRAERREVEN VAITALEGLK 200
    GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG MARDWPDARG 250
    IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE 300
    RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR 350
    LQKRGTGGVD TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK 400
    LERGQDIKVP PPLPQFGKK 419
    Length:419
    Mass (Da):47,504
    Last modified:October 17, 2006 - v2
    Checksum:i0CAF000074D99B6F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti74 – 741A → S in AAA60561. (PubMed:2324105)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05401 mRNA. Translation: AAA60561.1.
    CR450315 mRNA. Translation: CAG29311.1.
    BC029140 mRNA. Translation: AAH29140.1.
    CCDSiCCDS4053.1.
    PIRiA35756.
    RefSeqiNP_001093205.1. NM_001099735.1.
    NP_001093206.1. NM_001099736.1.
    NP_001816.2. NM_001825.2.
    UniGeneiHs.80691.

    Genome annotation databases

    EnsembliENST00000254035; ENSP00000254035; ENSG00000131730.
    ENST00000424301; ENSP00000404203; ENSG00000131730.
    ENST00000437669; ENSP00000410289; ENSG00000131730.
    GeneIDi1160.
    KEGGihsa:1160.
    UCSCiuc003khc.4. human.

    Polymorphism databases

    DMDMi116242603.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05401 mRNA. Translation: AAA60561.1 .
    CR450315 mRNA. Translation: CAG29311.1 .
    BC029140 mRNA. Translation: AAH29140.1 .
    CCDSi CCDS4053.1.
    PIRi A35756.
    RefSeqi NP_001093205.1. NM_001099735.1.
    NP_001093206.1. NM_001099736.1.
    NP_001816.2. NM_001825.2.
    UniGenei Hs.80691.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2GL6 X-ray 2.30 A/B/C/D/E/F/G/H 27-416 [» ]
    ProteinModelPortali P17540.
    SMRi P17540. Positions 47-413.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107580. 8 interactions.
    IntActi P17540. 6 interactions.
    MINTi MINT-5004086.
    STRINGi 9606.ENSP00000254035.

    Chemistry

    DrugBanki DB00148. Creatine.

    PTM databases

    PhosphoSitei P17540.

    Polymorphism databases

    DMDMi 116242603.

    2D gel databases

    UCD-2DPAGE P17540.

    Proteomic databases

    MaxQBi P17540.
    PaxDbi P17540.
    PRIDEi P17540.

    Protocols and materials databases

    DNASUi 1160.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000254035 ; ENSP00000254035 ; ENSG00000131730 .
    ENST00000424301 ; ENSP00000404203 ; ENSG00000131730 .
    ENST00000437669 ; ENSP00000410289 ; ENSG00000131730 .
    GeneIDi 1160.
    KEGGi hsa:1160.
    UCSCi uc003khc.4. human.

    Organism-specific databases

    CTDi 1160.
    GeneCardsi GC05P080564.
    HGNCi HGNC:1996. CKMT2.
    HPAi HPA051880.
    MIMi 123295. gene.
    neXtProti NX_P17540.
    PharmGKBi PA26534.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG3869.
    HOGENOMi HOG000232165.
    HOVERGENi HBG001339.
    InParanoidi P17540.
    KOi K00933.
    OMAi GRGWEFM.
    OrthoDBi EOG7XM2XW.
    PhylomeDBi P17540.
    TreeFami TF314214.

    Enzyme and pathway databases

    BioCyci MetaCyc:HS05555-MONOMER.
    Reactomei REACT_813. Creatine metabolism.

    Miscellaneous databases

    EvolutionaryTracei P17540.
    GeneWikii CKMT2.
    GenomeRNAii 1160.
    NextBioi 4814.
    PROi P17540.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P17540.
    Bgeei P17540.
    CleanExi HS_CKMT2.
    Genevestigatori P17540.

    Family and domain databases

    Gene3Di 1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view ]
    Pfami PF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48034. SSF48034. 1 hit.
    PROSITEi PS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Separate nuclear genes encode sarcomere-specific and ubiquitous human mitochondrial creatine kinase isoenzymes."
      Haas R.C., Strauss A.W.
      J. Biol. Chem. 265:6921-6927(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "Isolation and characterization of the gene and cDNA encoding human mitochondrial creatine kinase."
      Haas R.C., Korenfeld C., Zhang Z., Perryman B., Roman D., Strauss A.W.
      J. Biol. Chem. 264:2890-2897(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 40-65.
    5. "Crystal structure of human sarcomeric mitochondrial creatine kinase."
      Structural genomics consortium (SGC)
      Submitted (APR-2006) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 27-416 IN COMPLEX WITH ADP.

    Entry informationi

    Entry nameiKCRS_HUMAN
    AccessioniPrimary (citable) accession number: P17540
    Secondary accession number(s): Q6ICS8, Q8N1E1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 148 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Mitochondrial creatine kinase binds cardiolipin.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3