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Protein

Creatine kinase S-type, mitochondrial

Gene

CKMT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225ATP1
Binding sitei270ATPPROSITE-ProRule annotation1
Binding sitei326ATP1
Binding sitei369ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 166ATP5
Nucleotide bindingi354 – 359ATP6

GO - Molecular functioni

GO - Biological processi

  • creatine metabolic process Source: Reactome
  • muscle contraction Source: ProtInc

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS05555-MONOMER
ReactomeiR-HSA-71288 Creatine metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase S-type, mitochondrial (EC:2.7.3.2)
Alternative name(s):
Basic-type mitochondrial creatine kinase
Short name:
Mib-CK
Sarcomeric mitochondrial creatine kinase
Short name:
S-MtCK
Gene namesi
Name:CKMT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000131730.15
HGNCiHGNC:1996 CKMT2
MIMi123295 gene
neXtProtiNX_P17540

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi1160
OpenTargetsiENSG00000131730
PharmGKBiPA26534

Chemistry databases

DrugBankiDB00148 Creatine

Polymorphism and mutation databases

BioMutaiCKMT2
DMDMi116242603

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 39Mitochondrion1 PublicationAdd BLAST39
ChainiPRO_000001659440 – 419Creatine kinase S-type, mitochondrialAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei255PhosphotyrosineBy similarity1
Modified residuei356PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP17540
MaxQBiP17540
PaxDbiP17540
PeptideAtlasiP17540
PRIDEiP17540

2D gel databases

UCD-2DPAGEiP17540

PTM databases

iPTMnetiP17540
PhosphoSitePlusiP17540

Expressioni

Tissue specificityi

Sarcomere-specific. Found only in heart and skeletal muscles.

Gene expression databases

BgeeiENSG00000131730
CleanExiHS_CKMT2
ExpressionAtlasiP17540 baseline and differential
GenevisibleiP17540 HS

Organism-specific databases

HPAiHPA051880

Interactioni

Subunit structurei

Exists as an octamer composed of four CKMT2 homodimers.1 Publication

Protein-protein interaction databases

BioGridi10758014 interactors.
IntActiP17540 7 interactors.
STRINGi9606.ENSP00000254035

Structurei

Secondary structure

1419
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi50 – 53Combined sources4
Helixi63 – 67Combined sources5
Helixi70 – 76Combined sources7
Helixi87 – 96Combined sources10
Beta strandi101 – 103Combined sources3
Helixi115 – 118Combined sources4
Helixi120 – 130Combined sources11
Turni131 – 133Combined sources3
Turni136 – 138Combined sources3
Helixi147 – 149Combined sources3
Turni157 – 159Combined sources3
Beta strandi160 – 169Combined sources10
Turni177 – 179Combined sources3
Helixi182 – 196Combined sources15
Helixi201 – 203Combined sources3
Beta strandi205 – 209Combined sources5
Helixi210 – 212Combined sources3
Helixi215 – 223Combined sources9
Helixi234 – 237Combined sources4
Turni238 – 248Combined sources11
Beta strandi250 – 254Combined sources5
Beta strandi257 – 278Combined sources22
Helixi280 – 300Combined sources21
Turni309 – 311Combined sources3
Helixi318 – 320Combined sources3
Beta strandi326 – 332Combined sources7
Helixi334 – 338Combined sources5
Helixi342 – 348Combined sources7
Beta strandi351 – 355Combined sources5
Beta strandi367 – 373Combined sources7
Beta strandi376 – 378Combined sources3
Helixi380 – 396Combined sources17
Helixi398 – 402Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Z9MX-ray2.10A/B/C/D/E/F/G/H27-416[»]
ProteinModelPortaliP17540
SMRiP17540
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17540

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 132Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST87
Domaini159 – 401Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 64Cardiolipin-bindingBy similarityAdd BLAST25

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3581 Eukaryota
COG3869 LUCA
GeneTreeiENSGT00550000074561
HOGENOMiHOG000232165
HOVERGENiHBG001339
InParanoidiP17540
KOiK00933
OMAiAKLCDKA
OrthoDBiEOG091G0HZ0
PhylomeDBiP17540
TreeFamiTF314214

Family and domain databases

Gene3Di1.10.135.101 hit
InterProiView protein in InterPro
IPR000749 ATP-guanido_PTrfase
IPR022415 ATP-guanido_PTrfase_AS
IPR022414 ATP-guanido_PTrfase_cat
IPR022413 ATP-guanido_PTrfase_N
IPR036802 ATP-guanido_PTrfase_N_sf
IPR014746 Gln_synth/guanido_kin_cat_dom
PANTHERiPTHR11547 PTHR11547, 1 hit
PfamiView protein in Pfam
PF00217 ATP-gua_Ptrans, 1 hit
PF02807 ATP-gua_PtransN, 1 hit
SUPFAMiSSF48034 SSF48034, 1 hit
SSF55931 SSF55931, 1 hit
PROSITEiView protein in PROSITE
PS00112 PHOSPHAGEN_KINASE, 1 hit
PS51510 PHOSPHAGEN_KINASE_C, 1 hit
PS51509 PHOSPHAGEN_KINASE_N, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17540-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIFSKLLT GRNASLLFAT MGTSVLTTGY LLNRQKVCAE VREQPRLFPP
60 70 80 90 100
SADYPDLRKH NNCMAECLTP AIYAKLRNKV TPNGYTLDQC IQTGVDNPGH
110 120 130 140 150
PFIKTVGMVA GDEESYEVFA DLFDPVIKLR HNGYDPRVMK HTTDLDASKI
160 170 180 190 200
TQGQFDEHYV LSSRVRTGRS IRGLSLPPAC TRAERREVEN VAITALEGLK
210 220 230 240 250
GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG MARDWPDARG
260 270 280 290 300
IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE
310 320 330 340 350
RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR
360 370 380 390 400
LQKRGTGGVD TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK
410
LERGQDIKVP PPLPQFGKK
Length:419
Mass (Da):47,504
Last modified:October 17, 2006 - v2
Checksum:i0CAF000074D99B6F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74A → S in AAA60561 (PubMed:2324105).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05401 mRNA Translation: AAA60561.1
CR450315 mRNA Translation: CAG29311.1
BC029140 mRNA Translation: AAH29140.1
CCDSiCCDS4053.1
PIRiA35756
RefSeqiNP_001093205.1, NM_001099735.1
NP_001093206.1, NM_001099736.1
NP_001816.2, NM_001825.2
UniGeneiHs.80691

Genome annotation databases

EnsembliENST00000254035; ENSP00000254035; ENSG00000131730
ENST00000424301; ENSP00000404203; ENSG00000131730
ENST00000437669; ENSP00000410289; ENSG00000131730
GeneIDi1160
KEGGihsa:1160
UCSCiuc003khc.5 human

Similar proteinsi

Entry informationi

Entry nameiKCRS_HUMAN
AccessioniPrimary (citable) accession number: P17540
Secondary accession number(s): Q6ICS8, Q8N1E1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 17, 2006
Last modified: March 28, 2018
This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome