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Reviewed, UniProtKB/Swiss-Prot P17540 (KCRS_HUMAN)

Last modified November 25, 2008. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Creatine kinase, sarcomeric mitochondrial
    EC=2.7.3.2
Alternative name(s):
    S-MtCK
    Basic-type mitochondrial creatine kinase
    Mib-CK
Gene names
Name: CKMT2
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activity

ATP + creatine = ADP + phosphocreatine.

Subunit structure

Exists as an octamer composed of four CKMT2 homodimers.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.

Tissue specificity

Sarcomere-specific. Found only in heart and skeletal muscles.

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin.

Sequence similarities

Belongs to the ATP:guanido phosphotransferase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3939Mitochondrion
Chain40 – 419380Creatine kinase, sarcomeric mitochondrial
PRO_0000016594

Regions

Nucleotide binding162 – 1665ATP
Nucleotide binding354 – 3596ATP
Region40 – 6425Cardiolipin-binding By similarity

Sites

Binding site2251ATP
Binding site2701ATP By similarity
Binding site3261ATP
Binding site3691ATP

Experimental info

Sequence conflict741A → S in AAA60561. Ref.1

Secondary structure

......................................................... 419
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P17540-1 [UniParc].

Last modified October 17, 2006. Version 2.
Checksum: 0CAF000074D99B6F

FASTA41947,504
        10         20         30         40         50         60 
MASIFSKLLT GRNASLLFAT MGTSVLTTGY LLNRQKVCAE VREQPRLFPP SADYPDLRKH 

        70         80         90        100        110        120 
NNCMAECLTP AIYAKLRNKV TPNGYTLDQC IQTGVDNPGH PFIKTVGMVA GDEESYEVFA 

       130        140        150        160        170        180 
DLFDPVIKLR HNGYDPRVMK HTTDLDASKI TQGQFDEHYV LSSRVRTGRS IRGLSLPPAC 

       190        200        210        220        230        240 
TRAERREVEN VAITALEGLK GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG 

       250        260        270        280        290        300 
MARDWPDARG IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE 

       310        320        330        340        350        360 
RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR LQKRGTGGVD 

       370        380        390        400        410 
TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK LERGQDIKVP PPLPQFGKK 

« Hide

References

« Hide 'large scale' references
[1]"Separate nuclear genes encode sarcomere-specific and ubiquitous human mitochondrial creatine kinase isoenzymes."
Haas R.C., Strauss A.W.
J. Biol. Chem. 265:6921-6927(1990) [PubMed: 2324105] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[2]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Isolation and characterization of the gene and cDNA encoding human mitochondrial creatine kinase."
Haas R.C., Korenfeld C., Zhang Z., Perryman B., Roman D., Strauss A.W.
J. Biol. Chem. 264:2890-2897(1989) [PubMed: 2914937] [Abstract]
Cited for: PROTEIN SEQUENCE OF 40-65.
[5]"Crystal structure of human sarcomeric mitochondrial creatine kinase."
Structural genomics consortium (SGC)
Submitted (APR-2006) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 27-416 IN COMPLEX WITH ADP.
+Additional computationally mapped references.

Cross-references

Sequence databases

J05401 mRNA. Translation: AAA60561.1.
CR450315 mRNA. Translation: CAG29311.1.
BC029140 mRNA. Translation: AAH29140.1.
PIRA35756.
RefSeqNP_001093205.1.
NP_001093206.1.
NP_001816.2.
UniGeneHs.80691

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2GL6X-ray2.30A/B/C/D/E/F/G/H27-416[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP17540.

PTM databases

PhosphoSiteP17540.

2-D gel databases

HSC-2DPAGEP17540.

Genome annotation databases

EnsemblENSG00000131730. Homo sapiens. [Contig view]
GeneID1160.
KEGGhsa:1160.

Organism-specific databases

H-InvDBHIX0004999.
HGNCHGNC:1996. CKMT2.
MIM123295. gene.
PharmGKBPA26534.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP17540.
HOVERGENP17540.

Enzyme and pathway databases

ReactomeREACT_13. Metabolism of amino acids.

Gene expression databases

ArrayExpressP17540.
CleanExHS_CKMT2.
GermOnlineENSG00000131730. Homo sapiens.

Family and domain databases

InterProIPR000749. ATP-gua_Ptrans.
IPR014746. Gln_synth/guanido_kin_cat.
[Graphical view]
Gene3DG3DSA:1.10.135.10. ATP-gua_Ptrans. 1 hit.
G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
PANTHERPTHR11547. ATP-gua_Ptrans. 1 hit.
PfamPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
PROSITEPS00112. GUANIDO_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00148. Creatine.
NextBio4814.
SOURCESearch...

Entry information

Entry nameKCRS_HUMAN
AccessionPrimary (citable) accession number: P17540
Secondary accession number(s): Q6ICS8, Q8N1E1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 17, 2006
Last modified: November 25, 2008
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents