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P17540 (KCRS_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Creatine kinase S-type, mitochondrial

EC=2.7.3.2
Alternative name(s):
Basic-type mitochondrial creatine kinase
Short name=Mib-CK
Sarcomeric mitochondrial creatine kinase
Short name=S-MtCK
Gene names
Name:CKMT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activity

ATP + creatine = ADP + phosphocreatine.

Subunit structure

Exists as an octamer composed of four CKMT2 homodimers.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.

Tissue specificity

Sarcomere-specific. Found only in heart and skeletal muscles.

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin.

Sequence similarities

Belongs to the ATP:guanido phosphotransferase family.

Contains 1 phosphagen kinase C-terminal domain.

Contains 1 phosphagen kinase N-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3939Mitochondrion Ref.4
Chain40 – 419380Creatine kinase S-type, mitochondrial
PRO_0000016594

Regions

Domain46 – 13287Phosphagen kinase N-terminal
Domain159 – 401243Phosphagen kinase C-terminal
Nucleotide binding162 – 1665ATP
Nucleotide binding354 – 3596ATP
Region40 – 6425Cardiolipin-binding By similarity

Sites

Binding site2251ATP
Binding site2701ATP By similarity
Binding site3261ATP
Binding site3691ATP

Experimental info

Sequence conflict741A → S in AAA60561. Ref.1

Secondary structure

....................................................... 419
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P17540 [UniParc].

Last modified October 17, 2006. Version 2.
Checksum: 0CAF000074D99B6F

FASTA41947,504
        10         20         30         40         50         60 
MASIFSKLLT GRNASLLFAT MGTSVLTTGY LLNRQKVCAE VREQPRLFPP SADYPDLRKH 

        70         80         90        100        110        120 
NNCMAECLTP AIYAKLRNKV TPNGYTLDQC IQTGVDNPGH PFIKTVGMVA GDEESYEVFA 

       130        140        150        160        170        180 
DLFDPVIKLR HNGYDPRVMK HTTDLDASKI TQGQFDEHYV LSSRVRTGRS IRGLSLPPAC 

       190        200        210        220        230        240 
TRAERREVEN VAITALEGLK GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG 

       250        260        270        280        290        300 
MARDWPDARG IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE 

       310        320        330        340        350        360 
RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR LQKRGTGGVD 

       370        380        390        400        410 
TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK LERGQDIKVP PPLPQFGKK 

« Hide

References

« Hide 'large scale' references
[1]"Separate nuclear genes encode sarcomere-specific and ubiquitous human mitochondrial creatine kinase isoenzymes."
Haas R.C., Strauss A.W.
J. Biol. Chem. 265:6921-6927(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[2]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Isolation and characterization of the gene and cDNA encoding human mitochondrial creatine kinase."
Haas R.C., Korenfeld C., Zhang Z., Perryman B., Roman D., Strauss A.W.
J. Biol. Chem. 264:2890-2897(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 40-65.
[5]"Crystal structure of human sarcomeric mitochondrial creatine kinase."
Structural genomics consortium (SGC)
Submitted (APR-2006) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 27-416 IN COMPLEX WITH ADP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05401 mRNA. Translation: AAA60561.1.
CR450315 mRNA. Translation: CAG29311.1.
BC029140 mRNA. Translation: AAH29140.1.
CCDSCCDS4053.1.
PIRA35756.
RefSeqNP_001093205.1. NM_001099735.1.
NP_001093206.1. NM_001099736.1.
NP_001816.2. NM_001825.2.
UniGeneHs.80691.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2GL6X-ray2.30A/B/C/D/E/F/G/H27-416[»]
ProteinModelPortalP17540.
SMRP17540. Positions 47-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107580. 7 interactions.
IntActP17540. 6 interactions.
MINTMINT-5004086.
STRING9606.ENSP00000254035.

Chemistry

DrugBankDB00148. Creatine.

PTM databases

PhosphoSiteP17540.

Polymorphism databases

DMDM116242603.

2D gel databases

UCD-2DPAGEP17540.

Proteomic databases

MaxQBP17540.
PaxDbP17540.
PRIDEP17540.

Protocols and materials databases

DNASU1160.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000254035; ENSP00000254035; ENSG00000131730.
ENST00000424301; ENSP00000404203; ENSG00000131730.
ENST00000437669; ENSP00000410289; ENSG00000131730.
GeneID1160.
KEGGhsa:1160.
UCSCuc003khc.4. human.

Organism-specific databases

CTD1160.
GeneCardsGC05P080564.
HGNCHGNC:1996. CKMT2.
HPAHPA051880.
MIM123295. gene.
neXtProtNX_P17540.
PharmGKBPA26534.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG3869.
HOGENOMHOG000232165.
HOVERGENHBG001339.
InParanoidP17540.
KOK00933.
OMAGRGWEFM.
OrthoDBEOG7XM2XW.
PhylomeDBP17540.
TreeFamTF314214.

Enzyme and pathway databases

BioCycMetaCyc:HS05555-MONOMER.
ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressP17540.
BgeeP17540.
CleanExHS_CKMT2.
GenevestigatorP17540.

Family and domain databases

Gene3D1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProIPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PfamPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMSSF48034. SSF48034. 1 hit.
PROSITEPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP17540.
GeneWikiCKMT2.
GenomeRNAi1160.
NextBio4814.
PROP17540.
SOURCESearch...

Entry information

Entry nameKCRS_HUMAN
AccessionPrimary (citable) accession number: P17540
Secondary accession number(s): Q6ICS8, Q8N1E1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 17, 2006
Last modified: July 9, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM