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Protein

Creatine kinase S-type, mitochondrial

Gene

CKMT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225ATP1
Binding sitei270ATPPROSITE-ProRule annotation1
Binding sitei326ATP1
Binding sitei369ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 166ATP5
Nucleotide bindingi354 – 359ATP6

GO - Molecular functioni

GO - Biological processi

  • creatine metabolic process Source: Reactome
  • muscle contraction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS05555-MONOMER.
ZFISH:HS05555-MONOMER.
ReactomeiR-HSA-71288. Creatine metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase S-type, mitochondrial (EC:2.7.3.2)
Alternative name(s):
Basic-type mitochondrial creatine kinase
Short name:
Mib-CK
Sarcomeric mitochondrial creatine kinase
Short name:
S-MtCK
Gene namesi
Name:CKMT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:1996. CKMT2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: Reactome
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi1160.
OpenTargetsiENSG00000131730.
PharmGKBiPA26534.

Chemistry databases

DrugBankiDB00148. Creatine.

Polymorphism and mutation databases

BioMutaiCKMT2.
DMDMi116242603.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 39Mitochondrion1 PublicationAdd BLAST39
ChainiPRO_000001659440 – 419Creatine kinase S-type, mitochondrialAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei255PhosphotyrosineBy similarity1
Modified residuei356PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP17540.
MaxQBiP17540.
PaxDbiP17540.
PeptideAtlasiP17540.
PRIDEiP17540.

2D gel databases

UCD-2DPAGEP17540.

PTM databases

iPTMnetiP17540.
PhosphoSitePlusiP17540.

Expressioni

Tissue specificityi

Sarcomere-specific. Found only in heart and skeletal muscles.

Gene expression databases

BgeeiENSG00000131730.
CleanExiHS_CKMT2.
ExpressionAtlasiP17540. baseline and differential.
GenevisibleiP17540. HS.

Organism-specific databases

HPAiHPA051880.

Interactioni

Subunit structurei

Exists as an octamer composed of four CKMT2 homodimers.1 Publication

Protein-protein interaction databases

BioGridi107580. 13 interactors.
IntActiP17540. 6 interactors.
MINTiMINT-5004086.
STRINGi9606.ENSP00000254035.

Structurei

Secondary structure

1419
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi50 – 53Combined sources4
Helixi63 – 67Combined sources5
Helixi70 – 76Combined sources7
Helixi87 – 96Combined sources10
Beta strandi101 – 103Combined sources3
Helixi115 – 118Combined sources4
Helixi120 – 130Combined sources11
Turni131 – 133Combined sources3
Turni136 – 138Combined sources3
Helixi147 – 149Combined sources3
Turni157 – 159Combined sources3
Beta strandi160 – 169Combined sources10
Turni177 – 179Combined sources3
Helixi182 – 196Combined sources15
Helixi201 – 203Combined sources3
Beta strandi205 – 209Combined sources5
Helixi210 – 212Combined sources3
Helixi215 – 223Combined sources9
Helixi234 – 237Combined sources4
Turni238 – 248Combined sources11
Beta strandi250 – 254Combined sources5
Beta strandi257 – 278Combined sources22
Helixi280 – 300Combined sources21
Turni309 – 311Combined sources3
Helixi318 – 320Combined sources3
Beta strandi326 – 332Combined sources7
Helixi334 – 338Combined sources5
Helixi342 – 348Combined sources7
Beta strandi351 – 355Combined sources5
Beta strandi367 – 373Combined sources7
Beta strandi376 – 378Combined sources3
Helixi380 – 396Combined sources17
Helixi398 – 402Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Z9MX-ray2.10A/B/C/D/E/F/G/H27-416[»]
ProteinModelPortaliP17540.
SMRiP17540.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17540.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 132Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST87
Domaini159 – 401Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 64Cardiolipin-bindingBy similarityAdd BLAST25

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP17540.
KOiK00933.
OMAiEYQELTM.
OrthoDBiEOG091G0HZ0.
PhylomeDBiP17540.
TreeFamiTF314214.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17540-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASIFSKLLT GRNASLLFAT MGTSVLTTGY LLNRQKVCAE VREQPRLFPP
60 70 80 90 100
SADYPDLRKH NNCMAECLTP AIYAKLRNKV TPNGYTLDQC IQTGVDNPGH
110 120 130 140 150
PFIKTVGMVA GDEESYEVFA DLFDPVIKLR HNGYDPRVMK HTTDLDASKI
160 170 180 190 200
TQGQFDEHYV LSSRVRTGRS IRGLSLPPAC TRAERREVEN VAITALEGLK
210 220 230 240 250
GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG MARDWPDARG
260 270 280 290 300
IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE
310 320 330 340 350
RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR
360 370 380 390 400
LQKRGTGGVD TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK
410
LERGQDIKVP PPLPQFGKK
Length:419
Mass (Da):47,504
Last modified:October 17, 2006 - v2
Checksum:i0CAF000074D99B6F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74A → S in AAA60561 (PubMed:2324105).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05401 mRNA. Translation: AAA60561.1.
CR450315 mRNA. Translation: CAG29311.1.
BC029140 mRNA. Translation: AAH29140.1.
CCDSiCCDS4053.1.
PIRiA35756.
RefSeqiNP_001093205.1. NM_001099735.1.
NP_001093206.1. NM_001099736.1.
NP_001816.2. NM_001825.2.
UniGeneiHs.80691.

Genome annotation databases

EnsembliENST00000254035; ENSP00000254035; ENSG00000131730.
ENST00000424301; ENSP00000404203; ENSG00000131730.
ENST00000437669; ENSP00000410289; ENSG00000131730.
GeneIDi1160.
KEGGihsa:1160.
UCSCiuc003khc.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05401 mRNA. Translation: AAA60561.1.
CR450315 mRNA. Translation: CAG29311.1.
BC029140 mRNA. Translation: AAH29140.1.
CCDSiCCDS4053.1.
PIRiA35756.
RefSeqiNP_001093205.1. NM_001099735.1.
NP_001093206.1. NM_001099736.1.
NP_001816.2. NM_001825.2.
UniGeneiHs.80691.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Z9MX-ray2.10A/B/C/D/E/F/G/H27-416[»]
ProteinModelPortaliP17540.
SMRiP17540.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107580. 13 interactors.
IntActiP17540. 6 interactors.
MINTiMINT-5004086.
STRINGi9606.ENSP00000254035.

Chemistry databases

DrugBankiDB00148. Creatine.

PTM databases

iPTMnetiP17540.
PhosphoSitePlusiP17540.

Polymorphism and mutation databases

BioMutaiCKMT2.
DMDMi116242603.

2D gel databases

UCD-2DPAGEP17540.

Proteomic databases

EPDiP17540.
MaxQBiP17540.
PaxDbiP17540.
PeptideAtlasiP17540.
PRIDEiP17540.

Protocols and materials databases

DNASUi1160.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254035; ENSP00000254035; ENSG00000131730.
ENST00000424301; ENSP00000404203; ENSG00000131730.
ENST00000437669; ENSP00000410289; ENSG00000131730.
GeneIDi1160.
KEGGihsa:1160.
UCSCiuc003khc.5. human.

Organism-specific databases

CTDi1160.
DisGeNETi1160.
GeneCardsiCKMT2.
HGNCiHGNC:1996. CKMT2.
HPAiHPA051880.
MIMi123295. gene.
neXtProtiNX_P17540.
OpenTargetsiENSG00000131730.
PharmGKBiPA26534.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP17540.
KOiK00933.
OMAiEYQELTM.
OrthoDBiEOG091G0HZ0.
PhylomeDBiP17540.
TreeFamiTF314214.

Enzyme and pathway databases

BioCyciMetaCyc:HS05555-MONOMER.
ZFISH:HS05555-MONOMER.
ReactomeiR-HSA-71288. Creatine metabolism.

Miscellaneous databases

EvolutionaryTraceiP17540.
GeneWikiiCKMT2.
GenomeRNAii1160.
PROiP17540.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131730.
CleanExiHS_CKMT2.
ExpressionAtlasiP17540. baseline and differential.
GenevisibleiP17540. HS.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCRS_HUMAN
AccessioniPrimary (citable) accession number: P17540
Secondary accession number(s): Q6ICS8, Q8N1E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: October 17, 2006
Last modified: November 2, 2016
This is version 165 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.