P17538 (CTRB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chymotrypsinogen B
EC=3.4.21.1

Cleaved into the following 3 chains:

1. Chymotrypsin B chain A
2. Chymotrypsin B chain B
3. Chymotrypsin B chain C

Gene names

Name:	CTRB1
Synonyms:	CTRB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	digestion Inferred from electronic annotation. Source: UniProtKB-KW extracellular matrix disassembly Traceable author statement. Source: Reactome proteolysis Non-traceable author statement. Source: UniProtKB
Cellular_component	extracellular region Non-traceable author statement. Source: UniProtKB extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18
	Chain	19 – 263	245	Chymotrypsinogen B		PRO_0000027638
	Chain	19 – 31	13	Chymotrypsin B chain A		PRO_0000027639
	Chain	34 – 164	131	Chymotrypsin B chain B		PRO_0000027640
	Chain	167 – 263	97	Chymotrypsin B chain C		PRO_0000027641
Regions
	Domain	34 – 261	228	Peptidase S1
Sites
	Active site	75	1	Charge relay system By similarity
	Active site	120	1	Charge relay system By similarity
	Active site	213	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	19 ↔ 140		By similarity
	Disulfide bond	60 ↔ 76		By similarity
	Disulfide bond	154 ↔ 219		By similarity
	Disulfide bond	186 ↔ 200		By similarity
	Disulfide bond	209 ↔ 238		By similarity
Natural variations
	Natural variant	222	1	D → H. Corresponds to variant rs8061550 [ dbSNP | Ensembl ].		VAR_057158
	Natural variant	250	1	T → A. Corresponds to variant rs4737 [ dbSNP | Ensembl ].		VAR_014566

Sequences

	Sequence		Length	Mass (Da)
	P17538 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 4C1C055A490B8701	FASTA	263	27,870
	10 20 30 40 50 60 MAFLWLLSCW ALLGTTFGCG VPAIHPVLSG LSRIVNGEDA VPGSWPWQVS LQDKTGFHFC 70 80 90 100 110 120 GGSLISEDWV VTAAHCGVRT SDVVVAGEFD QGSDEENIQV LKIAKVFKNP KFSILTVNND 130 140 150 160 170 180 ITLLKLATPA RFSQTVSAVC LPSADDDFPA GTLCATTGWG KTKYNANKTP DKLQQAALPL 190 200 210 220 230 240 LSNAECKKSW GRRITDVMIC AGASGVSSCM GDSGGPLVCQ KDGAWTLVGI VSWGSDTCST 250 260 SSPGVYARVT KLIPWVQKIL AAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and nucleotide sequence of human pancreatic prechymotrypsinogen cDNA." Tomita N., Izumoto Y., Horii A., Doi S., Yokouchi H., Ogawa M., Mori T., Matsubara K. Biochem. Biophys. Res. Commun. 158:569-575(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas.
+	Additional computationally mapped references.

Web resources

Wikipedia

Chymotrypsin entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	M24400 mRNA. Translation: AAA52128.1. BT007356 mRNA. Translation: AAP36020.1. BC005385 mRNA. Translation: AAH05385.1.
IPI	IPI00015133.
PIR	A31299.
UniGene	Hs.610926.
3D structure databases
ProteinModelPortal	P17538.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000354294.
PTM databases
PhosphoSite	P17538.
Polymorphism databases
DMDM	117617.
Proteomic databases
PaxDb	P17538.
PRIDE	P17538.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361017; ENSP00000354294; ENSG00000168925.
UCSC	uc002fds.3. human.
Organism-specific databases
GeneCards	GC16P075252.
H-InvDB	HIX0013242.
HGNC	HGNC:2521. CTRB1.
MIM	118890. gene.
neXtProt	NX_P17538.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P17538.
OrthoDB	EOG4VMFG1.
Enzyme and pathway databases
Reactome	REACT_118779. Extracellular matrix organization.
Gene expression databases
Bgee	P17538.
CleanEx	HS_CTRB1.
Genevestigator	P17538.
GermOnline	ENSG00000168928. Homo sapiens.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P17538.
ChEMBL	CHEMBL4796.
SOURCE	Search...

Entry information

Entry name

CTRB1_HUMAN

Accession

Primary (citable) accession number: P17538

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	May 1, 2013
This is version 128 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.