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Protein

Tropomyosin-1

Gene

TPM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • actin lateral binding Source: SGD

GO - Biological processi

  • actin filament bundle assembly Source: SGD
  • actin filament organization Source: SGD
  • actin filament reorganization Source: SGD
  • actin polymerization or depolymerization Source: SGD
  • budding cell apical bud growth Source: SGD
  • budding cell isotropic bud growth Source: SGD
  • establishment of cell polarity Source: SGD
  • exocytosis Source: SGD
  • filamentous growth Source: SGD
  • intracellular mRNA localization Source: SGD
  • mitochondrion inheritance Source: SGD
  • pseudohyphal growth Source: SGD
  • vacuole inheritance Source: SGD
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33108-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tropomyosin-1
Gene namesi
Name:TPM1
Ordered Locus Names:YNL079C
ORF Names:N2332
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL079C.
SGDiS000005023. TPM1.

Subcellular locationi

GO - Cellular componenti

  • actin filament bundle Source: SGD
  • cellular bud neck contractile ring Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199Tropomyosin-1PRO_0000205692Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki39 – 39Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki59 – 59Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki187 – 187Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei195 – 1951PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP17536.

PTM databases

iPTMnetiP17536.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

  • actin lateral binding Source: SGD

Protein-protein interaction databases

BioGridi35744. 257 interactions.
DIPiDIP-2992N.
IntActiP17536. 3 interactions.
MINTiMINT-439244.

Structurei

3D structure databases

ProteinModelPortaliP17536.
SMRiP17536. Positions 1-62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 199199By similarityAdd
BLAST

Domaini

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00730000113677.
HOGENOMiHOG000180129.
InParanoidiP17536.
KOiK17945.
OMAiWERKNEE.
OrthoDBiEOG72RNBB.

Family and domain databases

InterProiIPR000533. Tropomyosin.
[Graphical view]
PfamiPF12718. Tropomyosin_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17536-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKIREKLSN LKLEAESWQE KYEELKEKNK DLEQENVEKE NQIKSLTVKN
60 70 80 90 100
QQLEDEIEKL EAGLSDSKQT EQDNVEKENQ IKSLTVKNHQ LEEEIEKLEA
110 120 130 140 150
ELAESKQLSE DSHHLQSNND NFSKKNQQLE EDLEESDTKL KETTEKLRES
160 170 180 190
DLKADQLERR VAALEEQREE WERKNEELTV KYEDAKKELD EIAASLENL
Length:199
Mass (Da):23,541
Last modified:August 1, 1990 - v1
Checksum:i845F3DB4387083BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25501 Genomic DNA. Translation: AAA35174.1.
DQ115393 Genomic DNA. Translation: AAZ22529.1.
X86470 Genomic DNA. Translation: CAA60179.1.
Z71355 Genomic DNA. Translation: CAA95953.1.
AY692838 Genomic DNA. Translation: AAT92857.1.
BK006947 Genomic DNA. Translation: DAA10466.1.
PIRiA32183.
RefSeqiNP_014320.1. NM_001182917.1.

Genome annotation databases

EnsemblFungiiYNL079C; YNL079C; YNL079C.
GeneIDi855645.
KEGGisce:YNL079C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25501 Genomic DNA. Translation: AAA35174.1.
DQ115393 Genomic DNA. Translation: AAZ22529.1.
X86470 Genomic DNA. Translation: CAA60179.1.
Z71355 Genomic DNA. Translation: CAA95953.1.
AY692838 Genomic DNA. Translation: AAT92857.1.
BK006947 Genomic DNA. Translation: DAA10466.1.
PIRiA32183.
RefSeqiNP_014320.1. NM_001182917.1.

3D structure databases

ProteinModelPortaliP17536.
SMRiP17536. Positions 1-62.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35744. 257 interactions.
DIPiDIP-2992N.
IntActiP17536. 3 interactions.
MINTiMINT-439244.

PTM databases

iPTMnetiP17536.

Proteomic databases

MaxQBiP17536.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL079C; YNL079C; YNL079C.
GeneIDi855645.
KEGGisce:YNL079C.

Organism-specific databases

EuPathDBiFungiDB:YNL079C.
SGDiS000005023. TPM1.

Phylogenomic databases

GeneTreeiENSGT00730000113677.
HOGENOMiHOG000180129.
InParanoidiP17536.
KOiK17945.
OMAiWERKNEE.
OrthoDBiEOG72RNBB.

Enzyme and pathway databases

BioCyciYEAST:G3O-33108-MONOMER.

Miscellaneous databases

PROiP17536.

Family and domain databases

InterProiIPR000533. Tropomyosin.
[Graphical view]
PfamiPF12718. Tropomyosin_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton."
    Liu H., Bretscher A.
    Cell 57:233-242(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
    Deutschbauer A.M., Davis R.W.
    Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK1.
  3. "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
    Poehlmann R., Philippsen P.
    Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-195, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-39; LYS-59 AND LYS-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTPM1_YEAST
AccessioniPrimary (citable) accession number: P17536
Secondary accession number(s): D6W1A0, Q45TY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 6, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.