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P17532

- TPH1_MOUSE

UniProt

P17532 - TPH1_MOUSE

Protein

Tryptophan 5-hydroxylase 1

Gene

Tph1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.

    Cofactori

    Fe2+ ion.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei238 – 2381TryptophanBy similarity
    Binding sitei260 – 2601TryptophanBy similarity
    Binding sitei268 – 2681TryptophanBy similarity
    Metal bindingi275 – 2751IronBy similarity
    Metal bindingi280 – 2801IronBy similarity
    Metal bindingi320 – 3201IronBy similarity
    Binding sitei339 – 3391TryptophanBy similarity
    Binding sitei369 – 3691Tryptophan; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. tryptophan 5-monooxygenase activity Source: MGI

    GO - Biological processi

    1. aromatic amino acid family metabolic process Source: InterPro
    2. bone remodeling Source: MGI
    3. circadian rhythm Source: Ensembl
    4. mammary gland alveolus development Source: MGI
    5. negative regulation of ossification Source: Ensembl
    6. response to immobilization stress Source: Ensembl
    7. serotonin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Serotonin biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_218437. Serotonin and melatonin biosynthesis.
    UniPathwayiUPA00846; UER00799.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tryptophan 5-hydroxylase 1 (EC:1.14.16.4)
    Alternative name(s):
    Tryptophan 5-monooxygenase 1
    Gene namesi
    Name:Tph1
    Synonyms:Tph
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:98796. Tph1.

    Subcellular locationi

    GO - Cellular componenti

    1. neuron projection Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 447447Tryptophan 5-hydroxylase 1PRO_0000205569Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei61 – 611Phosphoserine; by PKASequence Analysis

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP17532.
    PRIDEiP17532.

    PTM databases

    PhosphoSiteiP17532.

    Expressioni

    Gene expression databases

    ArrayExpressiP17532.
    BgeeiP17532.
    CleanExiMM_TPH1.
    GenevestigatoriP17532.

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    3D structure databases

    ProteinModelPortaliP17532.
    SMRiP17532. Positions 6-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 9776ACTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ACT domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG3186.
    HOGENOMiHOG000233373.
    HOVERGENiHBG006841.
    InParanoidiP17532.
    KOiK00502.
    OMAiTWGTVFQ.
    OrthoDBiEOG7KM5T1.
    PhylomeDBiP17532.
    TreeFamiTF313327.

    Family and domain databases

    Gene3Di1.10.800.10. 1 hit.
    InterProiIPR002912. ACT_dom.
    IPR001273. ArAA_hydroxylase.
    IPR018301. ArAA_hydroxylase_Fe/CU_BS.
    IPR019774. Aromatic-AA_hydroxylase_C.
    IPR005963. Trp_5_mOase.
    IPR019773. Tyrosine_3-monooxygenase-like.
    [Graphical view]
    PANTHERiPTHR11473. PTHR11473. 1 hit.
    PfamiPF01842. ACT. 1 hit.
    PF00351. Biopterin_H. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000336. TH. 1 hit.
    PRINTSiPR00372. FYWHYDRXLASE.
    SUPFAMiSSF56534. SSF56534. 1 hit.
    TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
    PROSITEiPS51671. ACT. 1 hit.
    PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
    PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17532-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIEDNKENKE NKDHSSERGR VTLIFSLENE VGGLIKVLKI FQENHVSLLH    50
    IESRKSKQRN SEFEIFVDCD ISREQLNDIF PLLKSHATVL SVDSPDQLTA 100
    KEDVMETVPW FPKKISDLDF CANRVLLYGS ELDADHPGFK DNVYRRRRKY 150
    FAELAMNYKH GDPIPKIEFT EEEIKTWGTI FRELNKLYPT HACREYLRNL 200
    PLLSKYCGYR EDNIPQLEDV SNFLKERTGF SIRPVAGYLS PRDFLSGLAF 250
    RVFHCTQYVR HSSDPLYTPE PDTCHELLGH VPLLAEPSFA QFSQEIGLAS 300
    LGASEETVQK LATCYFFTVE FGLCKQDGQL RVFGAGLLSS ISELKHALSG 350
    HAKVKPFDPK IACKQECLIT SFQDVYFVSE SFEDAKEKMR EFAKTVKRPF 400
    GLKYNPYTQS VQVLRDTKSI TSAMNELRYD LDVISDALAR VTRWPSV 447
    Length:447
    Mass (Da):51,343
    Last modified:August 1, 1990 - v1
    Checksum:i16C839F22A138BCA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04758 mRNA. Translation: AAA63401.1.
    BC072582 mRNA. Translation: AAH72582.1.
    CCDSiCCDS21280.1.
    PIRiA34582.
    RefSeqiNP_001129556.1. NM_001136084.2.
    NP_033440.1. NM_009414.3.
    XP_006540850.1. XM_006540787.1.
    UniGeneiMm.248684.
    Mm.398047.

    Genome annotation databases

    EnsembliENSMUST00000049298; ENSMUSP00000037752; ENSMUSG00000040046.
    ENSMUST00000107669; ENSMUSP00000103296; ENSMUSG00000040046.
    GeneIDi21990.
    KEGGimmu:21990.
    UCSCiuc009gyq.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04758 mRNA. Translation: AAA63401.1 .
    BC072582 mRNA. Translation: AAH72582.1 .
    CCDSi CCDS21280.1.
    PIRi A34582.
    RefSeqi NP_001129556.1. NM_001136084.2.
    NP_033440.1. NM_009414.3.
    XP_006540850.1. XM_006540787.1.
    UniGenei Mm.248684.
    Mm.398047.

    3D structure databases

    ProteinModelPortali P17532.
    SMRi P17532. Positions 6-441.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P17532.

    Proteomic databases

    PaxDbi P17532.
    PRIDEi P17532.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000049298 ; ENSMUSP00000037752 ; ENSMUSG00000040046 .
    ENSMUST00000107669 ; ENSMUSP00000103296 ; ENSMUSG00000040046 .
    GeneIDi 21990.
    KEGGi mmu:21990.
    UCSCi uc009gyq.2. mouse.

    Organism-specific databases

    CTDi 7166.
    MGIi MGI:98796. Tph1.

    Phylogenomic databases

    eggNOGi COG3186.
    HOGENOMi HOG000233373.
    HOVERGENi HBG006841.
    InParanoidi P17532.
    KOi K00502.
    OMAi TWGTVFQ.
    OrthoDBi EOG7KM5T1.
    PhylomeDBi P17532.
    TreeFami TF313327.

    Enzyme and pathway databases

    UniPathwayi UPA00846 ; UER00799 .
    Reactomei REACT_218437. Serotonin and melatonin biosynthesis.

    Miscellaneous databases

    NextBioi 301724.
    PROi P17532.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P17532.
    Bgeei P17532.
    CleanExi MM_TPH1.
    Genevestigatori P17532.

    Family and domain databases

    Gene3Di 1.10.800.10. 1 hit.
    InterProi IPR002912. ACT_dom.
    IPR001273. ArAA_hydroxylase.
    IPR018301. ArAA_hydroxylase_Fe/CU_BS.
    IPR019774. Aromatic-AA_hydroxylase_C.
    IPR005963. Trp_5_mOase.
    IPR019773. Tyrosine_3-monooxygenase-like.
    [Graphical view ]
    PANTHERi PTHR11473. PTHR11473. 1 hit.
    Pfami PF01842. ACT. 1 hit.
    PF00351. Biopterin_H. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000336. TH. 1 hit.
    PRINTSi PR00372. FYWHYDRXLASE.
    SUPFAMi SSF56534. SSF56534. 1 hit.
    TIGRFAMsi TIGR01270. Trp_5_monoox. 1 hit.
    PROSITEi PS51671. ACT. 1 hit.
    PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
    PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization and chromosomal mapping of a cDNA encoding tryptophan hydroxylase from a mouse mastocytoma cell line."
      Stoll J., Kozak C.A., Goldman D.
      Genomics 7:88-96(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiTPH1_MOUSE
    AccessioniPrimary (citable) accession number: P17532
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3