Reviewed, UniProtKB/Swiss-Prot P17532 (TPH1_MOUSE)

Names and origin

Protein names

Recommended name:
    Tryptophan 5-hydroxylase 1
    EC=1.14.16.4
Alternative name(s):
    Tryptophan 5-monooxygenase 1

Gene names

Name:	Tph1
Synonyms:	Tph

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion.
Pathway	Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Subunit structure	Multimer of identical subunits.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Serotonin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW serotonin biosynthetic process from tryptophan Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 5-monooxygenase activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Tryptophan 5-hydroxylase 1		PRO_0000205569
Regions
	Domain	21 – 95	75	ACT
Sites
	Metal binding	275	1	Iron By similarity
	Metal binding	280	1	Iron By similarity
	Metal binding	320	1	Iron By similarity
Amino acid modifications
	Modified residue	61	1	Phosphoserine; by PKA Potential

Sequences

	Sequence		Length	Mass (Da)
	P17532-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 16C839F22A138BCA	FASTA	447	51,343
	10 20 30 40 50 60 MIEDNKENKE NKDHSSERGR VTLIFSLENE VGGLIKVLKI FQENHVSLLH IESRKSKQRN 70 80 90 100 110 120 SEFEIFVDCD ISREQLNDIF PLLKSHATVL SVDSPDQLTA KEDVMETVPW FPKKISDLDF 130 140 150 160 170 180 CANRVLLYGS ELDADHPGFK DNVYRRRRKY FAELAMNYKH GDPIPKIEFT EEEIKTWGTI 190 200 210 220 230 240 FRELNKLYPT HACREYLRNL PLLSKYCGYR EDNIPQLEDV SNFLKERTGF SIRPVAGYLS 250 260 270 280 290 300 PRDFLSGLAF RVFHCTQYVR HSSDPLYTPE PDTCHELLGH VPLLAEPSFA QFSQEIGLAS 310 320 330 340 350 360 LGASEETVQK LATCYFFTVE FGLCKQDGQL RVFGAGLLSS ISELKHALSG HAKVKPFDPK 370 380 390 400 410 420 IACKQECLIT SFQDVYFVSE SFEDAKEKMR EFAKTVKRPF GLKYNPYTQS VQVLRDTKSI 430 440 TSAMNELRYD LDVISDALAR VTRWPSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and chromosomal mapping of a cDNA encoding tryptophan hydroxylase from a mouse mastocytoma cell line." Stoll J., Kozak C.A., Goldman D. Genomics 7:88-96(1990) [PubMed: 2110547] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04758 mRNA. Translation: AAA63401.1. BC072582 mRNA. Translation: AAH72582.1.
IPI	IPI00109968.
PIR	A34582.
RefSeq	NP_001129556.1. NP_033440.1.
UniGene	Mm.248684
3D structure databases
HSSP	HSSP built from PDB template 1MLW based on UniProtKB P17752.
SMR	P17532. Positions 107-396.
ModBase	Search...
PTM databases
PhosphoSite	P17532.
Genome annotation databases
Ensembl	ENSMUSG00000040046. Mus musculus. [Contig view]
GeneID	21990.
KEGG	mmu:21990.
Organism-specific databases
MGI	MGI:98796. Tph1.
Phylogenomic databases
HOGENOM	P17532.
HOVERGEN	P17532.
OMA	P17532. TWGTVFQ.
Enzyme and pathway databases
BRENDA	1.14.16.4. 244.
Gene expression databases
ArrayExpress	P17532.
Bgee	P17532.
CleanEx	MM_TPH1.
GermOnline	ENSMUSG00000040046. Mus musculus.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005963. Trp_5_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01270. Trp_5_monoox. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301724.
SOURCE	Search...

Entry information

Entry name

TPH1_MOUSE

Accession

Primary (citable) accession number: P17532

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)