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Protein

Tryptophan 5-hydroxylase 1

Gene

Tph1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei238 – 2381TryptophanBy similarity
Binding sitei260 – 2601TryptophanBy similarity
Binding sitei268 – 2681TryptophanBy similarity
Metal bindingi275 – 2751IronBy similarity
Metal bindingi280 – 2801IronBy similarity
Metal bindingi320 – 3201IronBy similarity
Binding sitei339 – 3391TryptophanBy similarity
Binding sitei369 – 3691Tryptophan; via carbonyl oxygenBy similarity

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. tryptophan 5-monooxygenase activity Source: MGI

GO - Biological processi

  1. aromatic amino acid family metabolic process Source: InterPro
  2. bone remodeling Source: MGI
  3. circadian rhythm Source: Ensembl
  4. mammary gland alveolus development Source: MGI
  5. negative regulation of ossification Source: Ensembl
  6. positive regulation of fat cell differentiation Source: MGI
  7. response to immobilization stress Source: Ensembl
  8. serotonin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Serotonin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_218437. Serotonin and melatonin biosynthesis.
UniPathwayiUPA00846; UER00799.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan 5-hydroxylase 1 (EC:1.14.16.4)
Alternative name(s):
Tryptophan 5-monooxygenase 1
Gene namesi
Name:Tph1
Synonyms:Tph
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:98796. Tph1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. neuron projection Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Tryptophan 5-hydroxylase 1PRO_0000205569Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei61 – 611Phosphoserine; by PKASequence Analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP17532.
PRIDEiP17532.

PTM databases

PhosphoSiteiP17532.

Expressioni

Gene expression databases

BgeeiP17532.
CleanExiMM_TPH1.
ExpressionAtlasiP17532. baseline and differential.
GenevestigatoriP17532.

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP17532.
SMRiP17532. Positions 6-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 9776ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG3186.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP17532.
KOiK00502.
OMAiQNVLMYG.
OrthoDBiEOG7KM5T1.
PhylomeDBiP17532.
TreeFamiTF313327.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF01842. ACT. 1 hit.
PF00351. Biopterin_H. 1 hit.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEDNKENKE NKDHSSERGR VTLIFSLENE VGGLIKVLKI FQENHVSLLH
60 70 80 90 100
IESRKSKQRN SEFEIFVDCD ISREQLNDIF PLLKSHATVL SVDSPDQLTA
110 120 130 140 150
KEDVMETVPW FPKKISDLDF CANRVLLYGS ELDADHPGFK DNVYRRRRKY
160 170 180 190 200
FAELAMNYKH GDPIPKIEFT EEEIKTWGTI FRELNKLYPT HACREYLRNL
210 220 230 240 250
PLLSKYCGYR EDNIPQLEDV SNFLKERTGF SIRPVAGYLS PRDFLSGLAF
260 270 280 290 300
RVFHCTQYVR HSSDPLYTPE PDTCHELLGH VPLLAEPSFA QFSQEIGLAS
310 320 330 340 350
LGASEETVQK LATCYFFTVE FGLCKQDGQL RVFGAGLLSS ISELKHALSG
360 370 380 390 400
HAKVKPFDPK IACKQECLIT SFQDVYFVSE SFEDAKEKMR EFAKTVKRPF
410 420 430 440
GLKYNPYTQS VQVLRDTKSI TSAMNELRYD LDVISDALAR VTRWPSV
Length:447
Mass (Da):51,343
Last modified:August 1, 1990 - v1
Checksum:i16C839F22A138BCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04758 mRNA. Translation: AAA63401.1.
BC072582 mRNA. Translation: AAH72582.1.
CCDSiCCDS21280.1.
PIRiA34582.
RefSeqiNP_001129556.1. NM_001136084.2.
NP_033440.1. NM_009414.3.
XP_006540850.1. XM_006540787.1.
UniGeneiMm.248684.
Mm.398047.

Genome annotation databases

EnsembliENSMUST00000049298; ENSMUSP00000037752; ENSMUSG00000040046.
ENSMUST00000107669; ENSMUSP00000103296; ENSMUSG00000040046.
GeneIDi21990.
KEGGimmu:21990.
UCSCiuc009gyq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04758 mRNA. Translation: AAA63401.1.
BC072582 mRNA. Translation: AAH72582.1.
CCDSiCCDS21280.1.
PIRiA34582.
RefSeqiNP_001129556.1. NM_001136084.2.
NP_033440.1. NM_009414.3.
XP_006540850.1. XM_006540787.1.
UniGeneiMm.248684.
Mm.398047.

3D structure databases

ProteinModelPortaliP17532.
SMRiP17532. Positions 6-441.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP17532.

Proteomic databases

PaxDbiP17532.
PRIDEiP17532.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049298; ENSMUSP00000037752; ENSMUSG00000040046.
ENSMUST00000107669; ENSMUSP00000103296; ENSMUSG00000040046.
GeneIDi21990.
KEGGimmu:21990.
UCSCiuc009gyq.2. mouse.

Organism-specific databases

CTDi7166.
MGIiMGI:98796. Tph1.

Phylogenomic databases

eggNOGiCOG3186.
HOGENOMiHOG000233373.
HOVERGENiHBG006841.
InParanoidiP17532.
KOiK00502.
OMAiQNVLMYG.
OrthoDBiEOG7KM5T1.
PhylomeDBiP17532.
TreeFamiTF313327.

Enzyme and pathway databases

UniPathwayiUPA00846; UER00799.
ReactomeiREACT_218437. Serotonin and melatonin biosynthesis.

Miscellaneous databases

NextBioi301724.
PROiP17532.
SOURCEiSearch...

Gene expression databases

BgeeiP17532.
CleanExiMM_TPH1.
ExpressionAtlasiP17532. baseline and differential.
GenevestigatoriP17532.

Family and domain databases

Gene3Di1.10.800.10. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001273. ArAA_hydroxylase.
IPR018301. ArAA_hydroxylase_Fe/CU_BS.
IPR019774. Aromatic-AA_hydroxylase_C.
IPR005963. Trp_5_mOase.
IPR019773. Tyrosine_3-monooxygenase-like.
[Graphical view]
PANTHERiPTHR11473. PTHR11473. 1 hit.
PfamiPF01842. ACT. 1 hit.
PF00351. Biopterin_H. 1 hit.
[Graphical view]
PIRSFiPIRSF000336. TH. 1 hit.
PRINTSiPR00372. FYWHYDRXLASE.
SUPFAMiSSF56534. SSF56534. 1 hit.
TIGRFAMsiTIGR01270. Trp_5_monoox. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00367. BH4_AAA_HYDROXYL_1. 1 hit.
PS51410. BH4_AAA_HYDROXYL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization and chromosomal mapping of a cDNA encoding tryptophan hydroxylase from a mouse mastocytoma cell line."
    Stoll J., Kozak C.A., Goldman D.
    Genomics 7:88-96(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiTPH1_MOUSE
AccessioniPrimary (citable) accession number: P17532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 4, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.