P17525 (MCAPS_PLRV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Minor capsid protein P3-RTD

Alternative name(s):
P74
Readthrough protein
Short name=RT protein

Gene names

ORF Names:

ORF3/ORF5

Organism

Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) [Reference proteome]

Taxonomic identifier

12046 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Luteoviridae › Polerovirus › Potato leafroll virus

Virus host

Solanum tuberosum (Potato) [TaxID: 4113]

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Minor component of the viral capsid involved in aphid transmission. The RTD domain of the protein is exposed on the surface of the particle and determines the vector specificity and intestinal tropism in the aphid By similarity. This domain also determines the limitation to host phloem. Ref.2
Subcellular location	Minor capsid protein P3-RTD: Virion Potential.
Miscellaneous	This protein is translated as a fusion protein by episodic readthrough of the major coat protein termination codon. It is composed of the major capsid protein fused to a long C-terminal extension called the readthrough domain (RTD). Readthrough of the terminator codon TAG occurs between the codons for 208-Lys and 210-Val By similarity. The N-terminal region like those of many plant virus capsid proteins is highly basic. It has been suggested that these regions may be involved in protein-RNA interaction.
Sequence similarities	Belongs to the luteoviruses readthrough protein family.

Ontologies

Keywords
Cellular component	Virion
Coding sequence diversity	Alternative promoter usage RNA suppression of termination
Molecular function	Capsid protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]

Isoform Minor capsid protein P3/P5 (identifier: P17525-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform P7 (identifier: P17525-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-591: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 716	716	Minor capsid protein P3-RTD		PRO_0000222429
Regions
	Region	210 – 716	507	Readthrough domain (RTD)
	Compositional bias	18 – 66	49	Arg-rich
Natural variations
	Alternative sequence	1 – 591	591	Missing in isoform P7.		VSP_038673

Sequences

	Sequence		Length	Mass (Da)
	Isoform Minor capsid protein P3/P5 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6B65E9AE10449C42	FASTA	716	79,761
	10 20 30 40 50 60 MSTVVVKGNV NGGVQQPRMR RRQSLRRRAN RVQPVVMVTA PGQPRRRRRR RGGNRRSRRT 70 80 90 100 110 120 GVPRGRGSSE TFVFTKDNLV GNTQGSFTFG PSLSDCPAFK DGILKAYHEY KITSILLQFV 130 140 150 160 170 180 SEASSTSSGS IAYELDPHCK VSSLQSYVNK FQITKGGAKT YQARMINGVE WHDSSEDQCR 190 200 210 220 230 240 ILWKGNGKSS DSAGSFRVTI KVALQNPKYD SGSEPSPSPQ PTPTPTPQKH ERFIAYVGIP 250 260 270 280 290 300 MLTIQAREND DQIILGSLGS QRMKYIEDEN QNYTKFSSEY YSQSSMQAVP MYYFNVPKGQ 310 320 330 340 350 360 WSVDISCEGY QPTSSTSDPN RGRSDGMIAY SNADSDYWNV GEADGVKISK LRNDNTYRQG 370 380 390 400 410 420 HPELEINSCH FREGQLLERD ATISFHVEAP TDGRFFLVGP AIQKTAKYNY TISYGDWTDR 430 440 450 460 470 480 DMELGLITVV LDEHLEGTGS ANRVRRPPRE GHTYMASPHE PEGKPVGNKP RDETPIQTQE 490 500 510 520 530 540 RQPDQTPSDD VSDAGSVNSG GPTESLRLEF GVNSDSTYDA TVDGTDWPRI PPPRHPPEPR 550 560 570 580 590 600 VSGNSRTVTD FSSKADLLEN WDAEHFDPGY SKEDVAAATI IAHGSIQDGR SMLEKREENV 610 620 630 640 650 660 KNKTSSWKPP SLKAVSPAIA KLRSIRKSQP LEGGTLNKDA TDGVSSIGSG SLTGGTLKRK 670 680 690 700 710 ATIEERLLQT LTTEQRLWYE NFKKTNPPAA TQWLFEYQPP PQVDRNIAEK PFQGRK
	Isoform P7 [UniParc]. Checksum: 13BA60F145B547B1	FASTA	125	14,037
	10 20 30 40 50 60 MLEKREENVK NKTSSWKPPS LKAVSPAIAK LRSIRKSQPL EGGTLNKDAT DGVSSIGSGS 70 80 90 100 110 120 LTGGTLKRKA TIEERLLQTL TTEQRLWYEN FKKTNPPAAT QWLFEYQPPP QVDRNIAEKP FQGRK

References

[1]	"Nucleotide sequence of potato leafroll luteovirus RNA." Mayo M.A., Robinson D.J., Jolly C.A., Hyman L. J. Gen. Virol. 70:1037-1051(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"The C terminus of the polerovirus p5 readthrough domain limits virus infection to the phloem." Peter K.A., Gildow F., Palukaitis P., Gray S.M. J. Virol. 83:5419-5429(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00530 Genomic RNA. No translation available.
PIR	WMVQ53. JA0122. S24594.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001517. Luteo_coat. IPR002929. PLrV_ORF5. [Graphical view]
Pfam	PF00894. Luteo_coat. 1 hit. PF01690. PLRV_ORF5. 1 hit. [Graphical view]
PRINTS	PR00910. LVIRUSORF6.
ProtoNet	Search...

Entry information

Entry name

MCAPS_PLRV1

Accession

Primary (citable) accession number: P17525

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program