P17520 (RDRP_PLRV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein P1-P2

Cleaved into the following 2 chains:

1. Serine protease
   EC=3.4.21.-
2. RNA-directed RNA polymerase
   EC=2.7.7.48
   Alternative name(s):
   69.6 kDa protein

Gene names

ORF Names:

ORF1/ORF2

Organism

Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) [Reference proteome]

Taxonomic identifier

12046 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Luteoviridae › Polerovirus › Potato leafroll virus

Virus host

Solanum tuberosum (Potato) [TaxID: 4113]

Protein attributes

Sequence length	1062 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted

General annotation (Comments)

Function	Precursor from which the RNA-dependent RNA polymerase (RdRp) is probably released. RNA-dependent RNA polymerase plays an essential role in virus replication Potential.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Protein P1-P2: Membrane; Multi-pass membrane protein Potential.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins. The protease probably cleaves itself and releases the RdRp Potential. Cleavages have been shown in the P1 protein, but since the N-terminus containing the serine protease is shared between P1 and P1-P2, cleavages should also occur within the P1-P2 protein.
Sequence similarities	Contains 1 RdRp catalytic domain.
Sequence caution	The sequence BAA00418.1 differs from that shown. Reason: Ribosomal frameshifting not described. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Viral RNA replication
Cellular component	Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Signal Transmembrane Transmembrane helix
Ligand	Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Serine protease Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type exopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform RNA-directed RNA polymerase (identifier: P17520-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by -1 ribosomal frameshifting between codons 487 and 488.

Isoform Protein P1 (identifier: P17519-1)

The sequence of this isoform can be found in the external entry P17519.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 1062	1042	Protein P1-P2		PRO_0000222399
	Chain	205 – 399	195	Serine protease Potential		PRO_0000390905
	Chain	400 – 1062	663	RNA-directed RNA polymerase Potential		PRO_0000390906
Regions
	Transmembrane	121 – 141	21	Helical; Potential
	Transmembrane	144 – 164	21	Helical; Potential
	Transmembrane	172 – 192	21	Helical; Potential
	Domain	859 – 974	116	RdRp catalytic
	Compositional bias	68 – 71	4	Poly-Pro
Sites
	Active site	255	1	By similarity
	Active site	286	1	By similarity
	Active site	354	1	By similarity
	Site	204 – 205	2	Cleavage; by viral serine protease Potential
	Site	399 – 400	2	Cleavage; by viral serine protease By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform RNA-directed RNA polymerase [UniParc]. Last modified January 19, 2010. Version 2. Checksum: 842C778B71EFDBA5	FASTA	1,062	118,730
	10 20 30 40 50 60 MNRFTAYAAL FFMFSLCSTA KEAGFLHPAF NFRGTSTMSA SSGDYSAAPT PLYKSWALPS 70 80 90 100 110 120 SLNLTTQPPP PLTDRSYYEL VQALTSKMRL DCQTVGDMTW RHLSEMLFAS WNSVKEVSLK 130 140 150 160 170 180 AASVTLWAII NIWFGLYWTL ARLITLFLWT FSIEALCLIL LGCITSLIYK GALSLSEHLP 190 200 210 220 230 240 VFLFMSPLKI IWRAAFSKRN YKNERAVEGY KGFSVPQKPP KSAVIELQHE NGSHLGYANC 250 260 270 280 290 300 IRLYSGENAL VTAEHCLEGA FATSLKTGNR IPMSTFFPIF KSARNDISIL VGPPNWEGLL 310 320 330 340 350 360 SVKGAHFITA DKIGKGPASF YTLEKGEWMC HSATIDGAHH QFVSVLCNTG PGYSGTGFWS 370 380 390 400 410 420 SKNLLGVLKG FPLEEECNYN VMSVIPSIPG ITSPNYVFES TAVKGRVFSD EAVKELEREA 430 440 450 460 470 480 SEAVKKLARF KSLTDKNWAD DYDSDEDYGL EREAATNAPA EKTAQTNSAE KTAPSTSAEK 490 500 510 520 530 540 TALTNKPFKW ASGTVRQNKR QLRHPRRRYK RTTNGQNGRT DHHSYGGENQ SLGDRGEDSE 550 560 570 580 590 600 QGVSESPAEA QTKEARKAWR EEQAKQFTSY FNAIYKWGAQ EGGCPPGFRK CGHIPRYYHP 610 620 630 640 650 660 RTRGETQWGQ KLCQVHPELA DKTAGFGWPK AGSEAELQSL NLQAARWLQR AESATIPGAE 670 680 690 700 710 720 ARKRVIEKTV EAYRNCVTNA PLCSLKSKLD WAGFQQDIRE AVQSLELDAG VGIPYIAYGL 730 740 750 760 770 780 PAHRGWVEDH KLLPVLTQLT FDRLQKMSEA SFEDMSAEEL VQEGLCDPIR LFVKGEPHKQ 790 800 810 820 830 840 SKLDEGRYRL IMSVSLVDQL VARVLFQNQN KREISLWRSV PSKPGFGLST DTQTAEFLEC 850 860 870 880 890 900 LQKVSGAPSV EELCANHKEY TRPTDCSGFD WSVAYWMLED DMEVRNRLTF NNTQLTKRLR 910 920 930 940 950 960 AAWLKCIGNS VLCLSDGTLL AQTVPGVQKS GSYNTSSSNS RIRVMAAYHC GADWAMAMGD 970 980 990 1000 1010 1020 DALEAPNSDL EEYKTLGFKV EVGRELEFCS HIFRNPTLAV PVNTNKMLYK LIHGYNPECG 1030 1040 1050 1060 NPEVIQNYLA AVFSVLQELR HDRELVAKLH QWLVPSATTK EH
	Isoform Protein P1 [UniParc]. See P17519.		–	–

References

[1]	"Nucleotide sequence of potato leafroll luteovirus RNA." Mayo M.A., Robinson D.J., Jolly C.A., Hyman L. J. Gen. Virol. 70:1037-1051(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Ribosomal frameshifting in plants: a novel signal directs the -1 frameshift in the synthesis of the putative viral replicase of potato leafroll luteovirus." Prufer D., Tacke E., Schmitz J., Kull B., Kaufmann A., Rohde W. EMBO J. 11:1111-1117(1992) [PubMed] [Europe PMC] [Abstract] Cited for: RIBOSOMAL FRAMESHIFTING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00530 Genomic RNA. Translation: BAA00418.1. Sequence problems.
PIR	RRVQLL. JA0119.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018019. Luteovirus_Orf2. IPR000382. Peptidase_S39B_luteovirus. IPR001795. RNA-dir_pol_luteovirus. IPR007094. RNA-dir_pol_PSvirus. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF02122. Peptidase_S39. 1 hit. PF02123. RdRP_4. 1 hit. [Graphical view]
PRINTS	PR00913. LVIRUSORF2.
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_PLRV1

Accession

Primary (citable) accession number: P17520

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 19, 2010
Last modified:	April 3, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program