P17519 (P1_PLRV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein P1

Alternative name(s):
69.7 kDa protein
Genome-linked protein precursor
Protein ORF1

Cleaved into the following 2 chains:

1. Serine protease
   EC=3.4.21.-
2. VPg/P1-C25

Gene names

ORF Names:

ORF1

Organism

Potato leafroll virus (strain Potato/Scotland/strain 1/1984) (PLrV) [Reference proteome]

Taxonomic identifier

12046 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Luteoviridae › Polerovirus › Potato leafroll virus

Virus host

Solanum tuberosum (Potato) [TaxID: 4113]

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Precursor from which the VPg molecule is probably released at the onset of the RNA synthesis. Essential for virus replication.
Subcellular location	Protein P1: Membrane; Multi-pass membrane protein Potential.
Domain	The C-terminus part of protein P1 and VPg/P1-C25 displays RNA-binding properties.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins. The protease probably cleaves itself and releases the VPg protein. The VPg protein is probably further cleaved in its C-terminus. Ref.3
Sequence similarities	Belongs to the peptidase S39B family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Signal Transmembrane Transmembrane helix
Ligand	RNA-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW viral reproductive process Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type exopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform Protein P1 (identifier: P17519-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by conventional translation.

Isoform RNA-directed RNA polymerase (identifier: P17520-1)

The sequence of this isoform can be found in the external entry P17520.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by -1 ribosomal frameshifting between codons 487 and 488.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 639	619	Protein P1		PRO_0000222396
	Chain	205 – 399	195	Serine protease Potential		PRO_0000390899
	Chain	400 – 639	240	VPg/P1-C25 Potential		PRO_0000390900
Regions
	Transmembrane	121 – 141	21	Helical; Potential
	Transmembrane	144 – 164	21	Helical; Potential
	Transmembrane	172 – 192	21	Helical; Potential
	Compositional bias	68 – 71	4	Poly-Pro
Sites
	Active site	255	1	Probable
	Active site	286	1	Probable
	Active site	354	1	Probable
	Site	204 – 205	2	Cleavage; by viral serine protease Potential
	Site	399 – 400	2	Cleavage; by viral serine protease Probable
Experimental info
	Mutagenesis	255	1	H → L: Complete loss of viral genomic or subgenomic RNA production. Ref.5
	Mutagenesis	286	1	D → A: Complete loss of viral genomic or subgenomic RNA production. Ref.5
	Mutagenesis	354	1	S → A: Complete loss of viral genomic or subgenomic RNA production. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform Protein P1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: AFCF2FB393BEE097	FASTA	639	69,629
	10 20 30 40 50 60 MNRFTAYAAL FFMFSLCSTA KEAGFLHPAF NFRGTSTMSA SSGDYSAAPT PLYKSWALPS 70 80 90 100 110 120 SLNLTTQPPP PLTDRSYYEL VQALTSKMRL DCQTVGDMTW RHLSEMLFAS WNSVKEVSLK 130 140 150 160 170 180 AASVTLWAII NIWFGLYWTL ARLITLFLWT FSIEALCLIL LGCITSLIYK GALSLSEHLP 190 200 210 220 230 240 VFLFMSPLKI IWRAAFSKRN YKNERAVEGY KGFSVPQKPP KSAVIELQHE NGSHLGYANC 250 260 270 280 290 300 IRLYSGENAL VTAEHCLEGA FATSLKTGNR IPMSTFFPIF KSARNDISIL VGPPNWEGLL 310 320 330 340 350 360 SVKGAHFITA DKIGKGPASF YTLEKGEWMC HSATIDGAHH QFVSVLCNTG PGYSGTGFWS 370 380 390 400 410 420 SKNLLGVLKG FPLEEECNYN VMSVIPSIPG ITSPNYVFES TAVKGRVFSD EAVKELEREA 430 440 450 460 470 480 SEAVKKLARF KSLTDKNWAD DYDSDEDYGL EREAATNAPA EKTAQTNSAE KTAPSTSAEK 490 500 510 520 530 540 TALTNKPLNG QAAPSAKTNG NSDIPDAATS APPMDKMVEQ IITAMVGRIN LSEIEEKIVS 550 560 570 580 590 600 RVSQKALQKP KQKKRGRRGG KNKQNSLPPT STQSTSGAPK KEAAPQASGS AGTSRATTTP 610 620 630 APEAKPSGGK NSAKFTPSWR IKQQDSAGQK PDLKLNSKA
	Isoform RNA-directed RNA polymerase [UniParc]. See P17520.		–	–

References

[1]	"Nucleotide sequence of potato leafroll luteovirus RNA." Mayo M.A., Robinson D.J., Jolly C.A., Hyman L. J. Gen. Virol. 70:1037-1051(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Ribosomal frameshifting in plants: a novel signal directs the -1 frameshift in the synthesis of the putative viral replicase of potato leafroll luteovirus." Prufer D., Tacke E., Schmitz J., Kull B., Kaufmann A., Rohde W. EMBO J. 11:1111-1117(1992) [PubMed] [Europe PMC] [Abstract] Cited for: RIBOSOMAL FRAMESHIFTING.
[3]	"The genome-linked protein of potato leafroll virus is located downstream of the putative protease domain of the ORF1 product." van der Wilk F., Verbeek M., Dullemans A.M., van den Heuvel J.F.J.M. Virology 234:300-303(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC PROCESSING OF POLYPROTEIN, IDENTIFICATION OF VPG, PROTEIN SEQUENCE OF 400-431.
[4]	"Immunological analysis of potato leafroll luteovirus (PLRV) P1 expression identifies a 25 kDa RNA-binding protein derived via P1 processing." Prufer D., Kawchuk L., Monecke M., Nowok S., Fischer R., Rohde W. Nucleic Acids Res. 27:421-425(1999) [PubMed] [Europe PMC] [Abstract] Cited for: RNA-BINDING.
[5]	"Mutational analysis of the proteinase function of Potato leafroll virus." Sadowy E., Juszczuk M., David C., Gronenborn B., Hulanicka M.D. J. Gen. Virol. 82:1517-1527(2001) [PubMed] [Europe PMC] [Abstract] Cited for: ACTIVE SITES OF SERINE PROTEASE, MUTAGENESIS OF HIS-255; ASP-286 AND SER-354. Strain: Isolate Polish.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00530 Genomic RNA. Translation: BAA00417.1.
PIR	WMVQ70. JA0118.
RefSeq	NP_056747.1. NC_001747.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1493894.
Family and domain databases
InterPro	IPR018019. Luteovirus_Orf2. IPR000382. Peptidase_S39B_luteovirus. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF02122. Peptidase_S39. 1 hit. [Graphical view]
PRINTS	PR00913. LVIRUSORF2.
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
ProtoNet	Search...

Entry information

Entry name

P1_PLRV1

Accession

Primary (citable) accession number: P17519

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program