P17513 (CHIP_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Acidic endochitinase P EC=3.2.1.14 Alternative name(s): Pathogenesis-related protein P Short name=PR-P
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted. Note: Extracellular fluid from leaves.
Induction	By TMV infection.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Pathogenesis-related protein
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW response to biotic stimulus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – 253	229	Acidic endochitinase P		PRO_0000005336
Amino acid modifications
	Disulfide bond	212 ↔ 244		By similarity
Natural variations
	Natural variant	199	1	C → Y in strain: cv. Samsun.

Sequences

	Sequence		Length	Mass (Da)
	P17513 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: AA49930C0A0C3E50	FASTA	253	27,469
	10 20 30 40 50 60 MEFSGSPLTL FCCVFFLFLT GSLAQGIGSI VTNDLFNEML KNRNDGRCPA NGFYTYDAFI 70 80 90 100 110 120 AAANSFPGFG TSGDDTARRK EIAAFFGQTS HETTGGSLSA EPFTGGYCFV RQNDQSDRYY 130 140 150 160 170 180 GRGPIQLTNQ NNYEKAGNAI RQDLVNNPDL VATDATISFK TAIWFWMTPQ DNKPSSHDVI 190 200 210 220 230 240 IGSWTPSAAD QSANRAPGCG VITNIINGGI ECGVGPNAAV EDRIGYYRRY CGMLNVAPGD 250 NLDCYNQRNF AQG

References

[1]	"Isolation of complementary DNA clones encoding pathogenesis-related proteins P and Q, two acidic chitinases from tobacco." Payne G., Ahl P., Moyer M., Harper A., Beck J., Meins F. Jr., Ryals J. Proc. Natl. Acad. Sci. U.S.A. 87:98-102(1990) [PubMed: 2296608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Xanthi NC.
[2]	"Analysis of acidic and basic chitinases from tobacco and petunia and their constitutive expression in transgenic tobacco." Linthorst H.J.M., van Loon L.C., van Rossum C.M.A., Mayer A., Bol J.F., van Roekel J., Meulenhof J., Conelissen B.J.C. Mol. Plant Microbe Interact. 3:252-258(1990) [PubMed: 2131096] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Samsun. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29869 mRNA. Translation: AAA34106.1. X51426 mRNA. Translation: CAA35790.1.
PIR	A34801. S20737.
3D structure databases
ProteinModelPortal	P17513.
SMR	P17513. Positions 26-251.
ModBase	Search...
Protein family/group databases
CAZy	GH19. Glycoside Hydrolase Family 19.
Proteomic databases
PRIDE	P17513.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. IPR023346. Lysozyme-like_dom. [Graphical view]
Pfam	PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
SUPFAM	SSF53955. SSF53955. 1 hit.
PROSITE	PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIP_TOBAC

Accession

Primary (citable) accession number: P17513

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	May 31, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program