ID   HXB6_HUMAN              Reviewed;         224 AA.
AC   P17509; A8K835; D3DTV5; P09068; Q9HB11; Q9UGH2;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 4.
DT   27-MAR-2024, entry version 220.
DE   RecName: Full=Homeobox protein Hox-B6;
DE   AltName: Full=Homeobox protein Hox-2.2;
DE   AltName: Full=Homeobox protein Hox-2B;
DE   AltName: Full=Homeobox protein Hu-2;
GN   Name=HOXB6; Synonyms=HOX2B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC   TISSUE=Placenta;
RX   PubMed=1672751; DOI=10.1093/nar/19.3.539;
RA   Shen W.-F., Detmer K., Simonitch-Eason T.A., Lawrence H.J., Largman C.;
RT   "Alternative splicing of the HOX 2.2 homeobox gene in human hematopoietic
RT   cells and murine embryonic and adult tissues.";
RL   Nucleic Acids Res. 19:539-545(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA   Frezza D., D'Esposito M., Migliaccio E., Santini S.M., Fruscalzo A.;
RT   "Expression of HOX genes in T lymphocytes and hairy leukemia cell lines.";
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Kidd K.K., Busygina V., DeMille M.M.C., Speed W.C., Ruggeri V., Kidd J.R.,
RA   Pakstis A.J.;
RT   "Overall linkage disequilibrium in 33 populations for highly informative
RT   multisite haplotypes spanning the HOXB gene cluster.";
RL   Am. J. Hum. Genet. 67:235-235(2000).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Colon;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 135-224.
RX   PubMed=2573064; DOI=10.1073/pnas.86.21.8536;
RA   Shen W.-F., Largman C., Lowney P., Corral J.C., Detmer K., Hauser C.A.,
RA   Simonitch T.A., Hack F.M., Lawrence H.J.;
RT   "Lineage-restricted expression of homeobox-containing genes in human
RT   hematopoietic cell lines.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:8536-8540(1989).
RN   [8]
RP   PRELIMINARY NUCLEOTIDE SEQUENCE OF 136-224.
RX   PubMed=6091895; DOI=10.1016/0092-8674(84)90261-7;
RA   Levine M., Rubin G.M., Tjian R.;
RT   "Human DNA sequences homologous to a protein coding region conserved
RT   between homeotic genes of Drosophila.";
RL   Cell 38:667-673(1984).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Embryonic kidney;
RX   PubMed=17525332; DOI=10.1126/science.1140321;
RA   Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E.,
RA   Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y.,
RA   Gygi S.P., Elledge S.J.;
RT   "ATM and ATR substrate analysis reveals extensive protein networks
RT   responsive to DNA damage.";
RL   Science 316:1160-1166(2007).
RN   [10]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19413330; DOI=10.1021/ac9004309;
RA   Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT   "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT   refined SCX-based approach.";
RL   Anal. Chem. 81:4493-4501(2009).
CC   -!- FUNCTION: Sequence-specific transcription factor which is part of a
CC       developmental regulatory system that provides cells with specific
CC       positional identities on the anterior-posterior axis.
CC   -!- INTERACTION:
CC       P17509; P49761: CLK3; NbExp=6; IntAct=EBI-741308, EBI-745579;
CC       P17509; P13807: GYS1; NbExp=3; IntAct=EBI-741308, EBI-740553;
CC       P17509; Q9HC98-4: NEK6; NbExp=3; IntAct=EBI-741308, EBI-11750983;
CC       P17509; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-741308, EBI-741158;
CC       P17509; P78362: SRPK2; NbExp=3; IntAct=EBI-741308, EBI-593303;
CC       P17509; Q96M29: TEKT5; NbExp=3; IntAct=EBI-741308, EBI-10239812;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=P17509-1; Sequence=Displayed;
CC       Name=2; Synonyms=Homeobox-less;
CC         IsoId=P17509-2; Sequence=VSP_002388, VSP_002389;
CC   -!- SIMILARITY: Belongs to the Antp homeobox family. {ECO:0000305}.
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DR   EMBL; X58431; CAA41335.1; -; Genomic_DNA.
DR   EMBL; X58431; CAA41336.1; -; Genomic_DNA.
DR   EMBL; AJ270993; CAB65909.1; -; mRNA.
DR   EMBL; AF287967; AAG31552.1; -; Genomic_DNA.
DR   EMBL; AK292200; BAF84889.1; -; mRNA.
DR   EMBL; CH471109; EAW94728.1; -; Genomic_DNA.
DR   EMBL; CH471109; EAW94729.1; -; Genomic_DNA.
DR   EMBL; CH471109; EAW94730.1; -; Genomic_DNA.
DR   EMBL; CH471109; EAW94731.1; -; Genomic_DNA.
DR   EMBL; BC014651; AAH14651.1; -; mRNA.
DR   EMBL; M30597; AAA36004.1; -; mRNA.
DR   EMBL; K02571; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS11531.1; -. [P17509-1]
DR   PIR; A05266; A05266.
DR   PIR; S26400; S26400.
DR   RefSeq; NP_061825.2; NM_018952.4. [P17509-1]
DR   RefSeq; XP_005257340.1; XM_005257283.3.
DR   RefSeq; XP_005257341.1; XM_005257284.3.
DR   AlphaFoldDB; P17509; -.
DR   SMR; P17509; -.
DR   BioGRID; 109456; 55.
DR   ELM; P17509; -.
DR   IntAct; P17509; 17.
DR   MINT; P17509; -.
DR   STRING; 9606.ENSP00000420009; -.
DR   iPTMnet; P17509; -.
DR   PhosphoSitePlus; P17509; -.
DR   BioMuta; HOXB6; -.
DR   DMDM; 116242515; -.
DR   jPOST; P17509; -.
DR   MassIVE; P17509; -.
DR   MaxQB; P17509; -.
DR   PaxDb; 9606-ENSP00000420009; -.
DR   PeptideAtlas; P17509; -.
DR   ProteomicsDB; 53478; -. [P17509-1]
DR   ProteomicsDB; 53479; -. [P17509-2]
DR   Pumba; P17509; -.
DR   Antibodypedia; 17852; 188 antibodies from 23 providers.
DR   DNASU; 3216; -.
DR   Ensembl; ENST00000225648.4; ENSP00000225648.3; ENSG00000108511.10. [P17509-1]
DR   Ensembl; ENST00000484302.3; ENSP00000420009.2; ENSG00000108511.10. [P17509-1]
DR   GeneID; 3216; -.
DR   KEGG; hsa:3216; -.
DR   MANE-Select; ENST00000225648.4; ENSP00000225648.3; NM_018952.5; NP_061825.2.
DR   UCSC; uc002ins.2; human. [P17509-1]
DR   AGR; HGNC:5117; -.
DR   CTD; 3216; -.
DR   DisGeNET; 3216; -.
DR   GeneCards; HOXB6; -.
DR   HGNC; HGNC:5117; HOXB6.
DR   HPA; ENSG00000108511; Tissue enhanced (epididymis, kidney).
DR   MIM; 142961; gene.
DR   neXtProt; NX_P17509; -.
DR   OpenTargets; ENSG00000108511; -.
DR   PharmGKB; PA29393; -.
DR   VEuPathDB; HostDB:ENSG00000108511; -.
DR   eggNOG; KOG0489; Eukaryota.
DR   GeneTree; ENSGT00940000161108; -.
DR   HOGENOM; CLU_061398_1_1_1; -.
DR   InParanoid; P17509; -.
DR   OMA; CAQDKNV; -.
DR   OrthoDB; 728401at2759; -.
DR   PhylomeDB; P17509; -.
DR   TreeFam; TF316310; -.
DR   PathwayCommons; P17509; -.
DR   SignaLink; P17509; -.
DR   SIGNOR; P17509; -.
DR   BioGRID-ORCS; 3216; 27 hits in 1184 CRISPR screens.
DR   ChiTaRS; HOXB6; human.
DR   GeneWiki; HOXB6; -.
DR   GenomeRNAi; 3216; -.
DR   Pharos; P17509; Tbio.
DR   PRO; PR:P17509; -.
DR   Proteomes; UP000005640; Chromosome 17.
DR   RNAct; P17509; Protein.
DR   Bgee; ENSG00000108511; Expressed in corpus epididymis and 119 other cell types or tissues.
DR   GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR   GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR   GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR   GO; GO:0048704; P:embryonic skeletal system morphogenesis; IEA:Ensembl.
DR   GO; GO:0034101; P:erythrocyte homeostasis; IEA:Ensembl.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017995; Homeobox_antennapedia.
DR   InterPro; IPR001827; Homeobox_Antennapedia_CS.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   PANTHER; PTHR45659; HOMEOBOX PROTEIN HOX; 1.
DR   PANTHER; PTHR45659:SF9; HOMEOBOX PROTEIN HOX-B6; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00025; ANTENNAPEDIA.
DR   PRINTS; PR00024; HOMEOBOX.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00032; ANTENNAPEDIA; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   Genevisible; P17509; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW   Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..224
FT                   /note="Homeobox protein Hox-B6"
FT                   /id="PRO_0000200135"
FT   DNA_BIND        146..205
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   MOTIF           127..132
FT                   /note="Antp-type hexapeptide"
FT   MOD_RES         214
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17525332"
FT   VAR_SEQ         140
FT                   /note="S -> E (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_002388"
FT   VAR_SEQ         141..224
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_002389"
FT   CONFLICT        24..25
FT                   /note="QL -> HV (in Ref. 1; CAA41335 and 2; CAB65909)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        33
FT                   /note="A -> R (in Ref. 1; CAA41335)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        60
FT                   /note="P -> R (in Ref. 1; CAA41335)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        73
FT                   /note="D -> A (in Ref. 1; CAA41335)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        149..150
FT                   /note="GR -> A (in Ref. 7; AAA36004)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   224 AA;  25432 MW;  F60292D4D664099C CRC64;
     MSSYFVNSTF PVTLASGQES FLGQLPLYSS GYADPLRHYP APYGPGPGQD KGFATSSYYP
     PAGGGYGRAA PCDYGPAPAF YREKESACAL SGADEQPPFH PEPRKSDCAQ DKSVFGETEE
     QKCSTPVYPW MQRMNSCNSS SFGPSGRRGR QTYTRYQTLE LEKEFHYNRY LTRRRRIEIA
     HALCLTERQI KIWFQNRRMK WKKESKLLSA SQLSAEEEEE KQAE
//