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Protein

Malate dehydrogenase, mitochondrial

Gene

MDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3).
Present with 28100 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50NADBy similarity1
Binding sitei99SubstratePROSITE-ProRule annotation1
Binding sitei105SubstratePROSITE-ProRule annotation1
Binding sitei112NADBy similarity1
Binding sitei137SubstratePROSITE-ProRule annotation1
Binding sitei171SubstratePROSITE-ProRule annotation1
Active sitei195Proton acceptorBy similarity1
Binding sitei245NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 30NADBy similarity7
Nucleotide bindingi135 – 137NADBy similarity3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: SGD
  • mRNA binding Source: SGD

GO - Biological processi

  • aerobic respiration Source: SGD
  • carbohydrate metabolic process Source: InterPro
  • chronological cell aging Source: SGD
  • malate metabolic process Source: InterPro
  • replicative cell aging Source: SGD
  • tricarboxylic acid cycle Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BioCyciYEAST:YKL085W-MONOMER
ReactomeiR-SCE-70263 Gluconeogenesis
R-SCE-71403 Citric acid cycle (TCA cycle)

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, mitochondrial (EC:1.1.1.37)
Gene namesi
Name:MDH1
Ordered Locus Names:YKL085W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL085W
SGDiS000001568 MDH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17Mitochondrion2 PublicationsAdd BLAST17
ChainiPRO_000001863318 – 334Malate dehydrogenase, mitochondrialAdd BLAST317

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177PhosphoserineCombined sources1
Modified residuei199PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP17505
PaxDbiP17505
PRIDEiP17505
TopDownProteomicsiP17505

2D gel databases

UCD-2DPAGEiP17505

PTM databases

iPTMnetiP17505

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
SSA2P105922EBI-10594,EBI-8603

Protein-protein interaction databases

BioGridi34048, 99 interactors
DIPiDIP-5734N
IntActiP17505, 34 interactors
STRINGi4932.YKL085W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HR9X-ray3.01O/P/Q/R2-9[»]
ProteinModelPortaliP17505
SMRiP17505
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17505

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000016686
HOGENOMiHOG000213792
InParanoidiP17505
KOiK00026
OMAiQCTPKVE
OrthoDBiEOG092C34N7

Family and domain databases

Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17505-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSRVAKRAF SSTVANPYKV TVLGAGGGIG QPLSLLLKLN HKVTDLRLYD
60 70 80 90 100
LKGAKGVATD LSHIPTNSVV KGFTPEEPDG LNNALKDTDM VLIPAGVPRK
110 120 130 140 150
PGMTRDDLFA INASIVRDLA AATAESAPNA AILVISNPVN STVPIVAQVL
160 170 180 190 200
KNKGVYNPKK LFGVTTLDSI RAARFISEVE NTDPTQERVN VIGGHSGITI
210 220 230 240 250
IPLISQTNHK LMSDDKRHEL IHRIQFGGDE VVKAKNGAGS ATLSMAHAGA
260 270 280 290 300
KFANAVLSGF KGERDVIEPS FVDSPLFKSE GIEFFASPVT LGPDGIEKIH
310 320 330
PIGELSSEEE EMLQKCKETL KKNIEKGVNF VASK
Length:334
Mass (Da):35,650
Last modified:June 1, 1994 - v2
Checksum:i8645C4CDFB9857C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68S → G AA sequence (PubMed:3552052).Curated1
Sequence conflicti306S → F in AAS56240 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02841 Genomic DNA Translation: AAA34759.1
Z28085 Genomic DNA Translation: CAA81923.1
AY557914 Genomic DNA Translation: AAS56240.1
BK006944 Genomic DNA Translation: DAA09073.1
PIRiA31945 DEBYMM
RefSeqiNP_012838.1, NM_001179651.1

Genome annotation databases

EnsemblFungiiYKL085W; YKL085W; YKL085W
GeneIDi853777
KEGGisce:YKL085W

Similar proteinsi

Entry informationi

Entry nameiMDHM_YEAST
AccessioniPrimary (citable) accession number: P17505
Secondary accession number(s): D6VXK3, E9P8U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health