Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P17505

- MDHM_YEAST

UniProt

P17505 - MDHM_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Malate dehydrogenase, mitochondrial

Gene

MDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501NADBy similarity
Binding sitei99 – 991SubstratePROSITE-ProRule annotation
Binding sitei105 – 1051SubstratePROSITE-ProRule annotation
Binding sitei112 – 1121NADBy similarity
Binding sitei137 – 1371SubstratePROSITE-ProRule annotation
Binding sitei171 – 1711SubstratePROSITE-ProRule annotation
Active sitei195 – 1951Proton acceptorBy similarity
Binding sitei245 – 2451NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 307NADBy similarity
Nucleotide bindingi135 – 1373NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: SGD
  2. mRNA binding Source: SGD

GO - Biological processi

  1. aerobic respiration Source: SGD
  2. cellular carbohydrate metabolic process Source: InterPro
  3. chronological cell aging Source: SGD
  4. malate metabolic process Source: InterPro
  5. replicative cell aging Source: SGD
  6. tricarboxylic acid cycle Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YKL085W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, mitochondrial (EC:1.1.1.37)
Gene namesi
Name:MDH1
Ordered Locus Names:YKL085W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL085w.
SGDiS000001568. MDH1.

Subcellular locationi

Mitochondrion matrix 1 Publication

GO - Cellular componenti

  1. mitochondrial matrix Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1717Mitochondrion2 PublicationsAdd
BLAST
Chaini18 – 334317Malate dehydrogenase, mitochondrialPRO_0000018633Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei177 – 1771Phosphoserine1 Publication
Modified residuei199 – 1991Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP17505.
PaxDbiP17505.
PeptideAtlasiP17505.
PRIDEiP17505.

2D gel databases

UCD-2DPAGEP17505.

Expressioni

Gene expression databases

GenevestigatoriP17505.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
SSA2P105922EBI-10594,EBI-8603

Protein-protein interaction databases

BioGridi34048. 67 interactions.
DIPiDIP-5734N.
IntActiP17505. 8 interactions.
MINTiMINT-697512.
STRINGi4932.YKL085W.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HR9X-ray3.01O/P/Q/R2-9[»]
ProteinModelPortaliP17505.
SMRiP17505. Positions 19-331.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17505.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0039.
GeneTreeiENSGT00390000016686.
HOGENOMiHOG000213792.
InParanoidiP17505.
KOiK00026.
OMAiISEVENT.
OrthoDBiEOG7GJ6Q7.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17505-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSRVAKRAF SSTVANPYKV TVLGAGGGIG QPLSLLLKLN HKVTDLRLYD
60 70 80 90 100
LKGAKGVATD LSHIPTNSVV KGFTPEEPDG LNNALKDTDM VLIPAGVPRK
110 120 130 140 150
PGMTRDDLFA INASIVRDLA AATAESAPNA AILVISNPVN STVPIVAQVL
160 170 180 190 200
KNKGVYNPKK LFGVTTLDSI RAARFISEVE NTDPTQERVN VIGGHSGITI
210 220 230 240 250
IPLISQTNHK LMSDDKRHEL IHRIQFGGDE VVKAKNGAGS ATLSMAHAGA
260 270 280 290 300
KFANAVLSGF KGERDVIEPS FVDSPLFKSE GIEFFASPVT LGPDGIEKIH
310 320 330
PIGELSSEEE EMLQKCKETL KKNIEKGVNF VASK
Length:334
Mass (Da):35,650
Last modified:June 1, 1994 - v2
Checksum:i8645C4CDFB9857C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681S → G AA sequence (PubMed:3552052)Curated
Sequence conflicti306 – 3061S → F in AAS56240. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02841 Genomic DNA. Translation: AAA34759.1.
Z28085 Genomic DNA. Translation: CAA81923.1.
AY557914 Genomic DNA. Translation: AAS56240.1.
BK006944 Genomic DNA. Translation: DAA09073.1.
PIRiA31945. DEBYMM.
RefSeqiNP_012838.1. NM_001179651.1.

Genome annotation databases

EnsemblFungiiYKL085W; YKL085W; YKL085W.
GeneIDi853777.
KEGGisce:YKL085W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02841 Genomic DNA. Translation: AAA34759.1 .
Z28085 Genomic DNA. Translation: CAA81923.1 .
AY557914 Genomic DNA. Translation: AAS56240.1 .
BK006944 Genomic DNA. Translation: DAA09073.1 .
PIRi A31945. DEBYMM.
RefSeqi NP_012838.1. NM_001179651.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HR9 X-ray 3.01 O/P/Q/R 2-9 [» ]
ProteinModelPortali P17505.
SMRi P17505. Positions 19-331.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34048. 67 interactions.
DIPi DIP-5734N.
IntActi P17505. 8 interactions.
MINTi MINT-697512.
STRINGi 4932.YKL085W.

2D gel databases

UCD-2DPAGE P17505.

Proteomic databases

MaxQBi P17505.
PaxDbi P17505.
PeptideAtlasi P17505.
PRIDEi P17505.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL085W ; YKL085W ; YKL085W .
GeneIDi 853777.
KEGGi sce:YKL085W.

Organism-specific databases

CYGDi YKL085w.
SGDi S000001568. MDH1.

Phylogenomic databases

eggNOGi COG0039.
GeneTreei ENSGT00390000016686.
HOGENOMi HOG000213792.
InParanoidi P17505.
KOi K00026.
OMAi ISEVENT.
OrthoDBi EOG7GJ6Q7.

Enzyme and pathway databases

BioCyci YEAST:YKL085W-MONOMER.

Miscellaneous databases

EvolutionaryTracei P17505.
NextBioi 974888.
PROi P17505.

Gene expression databases

Genevestigatori P17505.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene sequence and primary structure of mitochondrial malate dehydrogenase from Saccharomyces cerevisiae."
    Thompson L.M., Sutherland P., Steffan J.S., McAlister-Henn L.
    Biochemistry 27:8393-8400(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-33.
    Strain: S173-6B.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Purification procedure and N-terminal amino acid sequence of yeast malate dehydrogenase isoenzymes."
    Kopetzki E., Entian K.-D., Lottspeich F., Mecke D.
    Biochim. Biophys. Acta 912:398-403(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 18-73.
  6. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 55-62, SUBCELLULAR LOCATION.
    Strain: ATCC 201238 / W303-1B.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
    Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
    Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-177, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMDHM_YEAST
AccessioniPrimary (citable) accession number: P17505
Secondary accession number(s): D6VXK3, E9P8U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3).
Present with 28100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3