Reviewed,
UniProtKB/Swiss-Prot P17505 (MDHM_YEAST)
Last modified
June 16, 2009.
Version 93.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Malate dehydrogenase, mitochondrial EC=1.1.1.37 | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 334 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | (S)-malate + NAD+ = oxaloacetate + NADH. |
| Subunit structure | Homodimer. |
| Subcellular location | |
| Miscellaneous | Yeast contains at least 3 malate dehydrogenase isoenzymes: a mitochondrial (MDH1), a cytoplasmic (MDH2) and a peroxisomal (MDH3). Present with 28100 molecules/cell in log phase SD medium. Ref.5 |
| Sequence similarities | Belongs to the LDH/MDH superfamily. MDH type 1 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Tricarboxylic acid cycle |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| PTM | Phosphoprotein |
| Technical term | 3D-structure Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | chronological cell aging Inferred from mutant phenotype. Source: SGD glycolysisInferred from electronic annotation. Source: InterPro malate metabolic processInferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW replicative cell agingInferred from mutant phenotype. Source: SGD tricarboxylic acid cycleInferred from mutant phenotype. Source: SGD |
| Cellular component | mitochondrial matrix Inferred from direct assay. Source: SGD |
| Molecular function | L-malate dehydrogenase activity Inferred from direct assay. Source: SGD protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 17 | 17 | Mitochondrion Ref.1 Ref.3 | ||||||
| Chain | 18 – 334 | 317 | Malate dehydrogenase, mitochondrial | PRO_0000018633 | |||||
Regions | |||||||||
| Nucleotide binding | 24 – 30 | 7 | NAD By similarity | ||||||
| Nucleotide binding | 135 – 137 | 3 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 195 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 50 | 1 | NAD By similarity | ||||||
| Binding site | 99 | 1 | Substrate By similarity | ||||||
| Binding site | 105 | 1 | Substrate By similarity | ||||||
| Binding site | 112 | 1 | NAD By similarity | ||||||
| Binding site | 137 | 1 | Substrate By similarity | ||||||
| Binding site | 171 | 1 | Substrate By similarity | ||||||
| Binding site | 245 | 1 | NAD By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 177 | 1 | Phosphoserine Ref.6 | ||||||
Experimental info | |||||||||
| Sequence conflict | 68 | 1 | S → G AA sequence Ref.3 | ||||||
Sequences
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References
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| J02841 Genomic DNA. Translation: AAA34759.1. Z28085 Genomic DNA. Translation: CAA81923.1. | |||||||||||||
| PIR | DEBYMM. A31945. | ||||||||||||
| RefSeq | NP_012838.1. | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| DIP | DIP:5734N. | ||||||||||||
| IntAct | P17505. 32 interactions. | ||||||||||||
Proteomic databases | |||||||||||||
| PeptideAtlas | P17505. | ||||||||||||
| PRIDE | P17505. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | YKL085W. Saccharomyces cerevisiae. [Contig view] | ||||||||||||
| GeneID | 853777. | ||||||||||||
| GenomeReviews | Gene locus YKL085W in contig Y13137_GR. | ||||||||||||
| KEGG | sce:YKL085W. | ||||||||||||
| NMPDR | fig|4932.3.peg.3822. | ||||||||||||
Organism-specific databases | |||||||||||||
| CYGD | YKL085w. | ||||||||||||
| SGD | S000001568. MDH1. | ||||||||||||
| Yeast-GFP | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | P17505. | ||||||||||||
| OMA | P17505. VRSEETE. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BRENDA | 1.1.1.37. 250. | ||||||||||||
Gene expression databases | |||||||||||||
| GermOnline | YKL085W. Saccharomyces cerevisiae. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk_g_bac. IPR016040. NAD(P)-bd_dom. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. | ||||||||||||
| PANTHER | PTHR11540:SF1. MDH_euk_g_bac. 1 hit. | ||||||||||||
| Pfam | PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view] | ||||||||||||
| PIRSF | PIRSF000102. Lac_mal_DH. 1 hit. | ||||||||||||
| TIGRFAMs | TIGR01772. MDH_euk_gproteo. 1 hit. | ||||||||||||
| PROSITE | PS00068. MDH. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 974888. | ||||||||||||
Entry information
| Entry name | MDHM_YEAST | ||||||||
| Accession | Primary (citable) accession number: P17505 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XI Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names |

Clusters with


