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Protein

Major envelope glycoprotein

Gene

GP64

Organism
Autographa californica nuclear polyhedrosis virus (AcMNPV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Class III viral fusion protein. Envelope phosphoglycoprotein which mediates the fusion of viral and host endosomal membranes leading to virus entry into the host cell. After receptor-mediated internalization of the virus, gp64 undergoes conformational change into a fusion-competent state at low pH, and the nucleocapsid is released into the cytoplasm after cell fusion. May also play role in budding.3 Publications

GO - Biological processi

  • fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  • membrane fusion involved in viral entry into host cell Source: UniProtKB
  • modulation by virus of host morphology or physiology Source: InterPro
  • viral budding from plasma membrane Source: UniProtKB
  • viral entry into host cell Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Viral penetration into host cytoplasm, Virus entry into host cell

Protein family/group databases

TCDBi1.G.15.1.1. the autographa californica nuclear polyhedrosis virus major envelope glycoprotein gp64 (gp64) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Major envelope glycoprotein
Alternative name(s):
gp64
Gene namesi
Name:GP64
Synonyms:GP67
ORF Names:ORF128
OrganismiAutographa californica nuclear polyhedrosis virus (AcMNPV)
Taxonomic identifieri46015 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageBaculoviridaeAlphabaculovirus
Virus hostiLepidoptera (butterflies and moths) [TaxID: 7088]
Proteomesi
  • UP000008292 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei482 – 504HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

  • host cell plasma membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • viral envelope Source: UniProtKB
  • virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi503C → A or S: Abolishes palmitoylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003674621 – 512Major envelope glycoproteinAdd BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24Interchain (with C-372 in trimeric partner 1)1 Publication
Disulfide bondi111 ↔ 1641 Publication
Disulfide bondi128 ↔ 1581 Publication
Glycosylationi159N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi177 ↔ 1841 Publication
Glycosylationi197N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi228 ↔ 2461 Publication
Disulfide bondi262 ↔ 2671 Publication
Glycosylationi354N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi372Interchain (with C-24 in trimeric partner 2)1 Publication
Disulfide bondi382 ↔ 4021 Publication
Glycosylationi384N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi426N-linked (GlcNAc...); by hostSequence analysis1
Lipidationi503S-palmitoyl cysteine; by host1 Publication1

Post-translational modificationi

Palmitoylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

PTM databases

SwissPalmiP17501.

Interactioni

Subunit structurei

Homotrimer; disulfide-linked.2 Publications

Protein-protein interaction databases

DIPiDIP-46361N.

Structurei

Secondary structure

1512
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 28Combined sources3
Beta strandi44 – 58Combined sources15
Beta strandi60 – 78Combined sources19
Turni80 – 82Combined sources3
Beta strandi87 – 95Combined sources9
Helixi99 – 108Combined sources10
Beta strandi111 – 113Combined sources3
Beta strandi117 – 121Combined sources5
Helixi122 – 126Combined sources5
Helixi127 – 130Combined sources4
Beta strandi136 – 140Combined sources5
Beta strandi146 – 148Combined sources3
Beta strandi155 – 177Combined sources23
Turni179 – 181Combined sources3
Beta strandi184 – 189Combined sources6
Beta strandi200 – 202Combined sources3
Beta strandi204 – 206Combined sources3
Beta strandi209 – 215Combined sources7
Beta strandi219 – 231Combined sources13
Beta strandi243 – 250Combined sources8
Beta strandi252 – 254Combined sources3
Beta strandi261 – 263Combined sources3
Beta strandi266 – 270Combined sources5
Helixi299 – 342Combined sources44
Helixi348 – 352Combined sources5
Beta strandi357 – 365Combined sources9
Beta strandi367 – 371Combined sources5
Turni390 – 392Combined sources3
Helixi433 – 435Combined sources3
Helixi437 – 452Combined sources16
Beta strandi455 – 458Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DUZX-ray2.95A21-499[»]
ProteinModelPortaliP17501.
SMRiP17501.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17501.

Family & Domainsi

Sequence similaritiesi

Belongs to the baculoviridae gp64 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR004955. Baculovirus_Gp64.
[Graphical view]
PfamiPF03273. Baculo_gp64. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17501-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSAIVLYVL LAAAAHSAFA AEHCNAQMKT GPYKIKNLDI TPPKETLQKD
60 70 80 90 100
VEITIVETDY NENVIIGYKG YYQAYAYNGG SLDPNTRVEE TMKTLNVGKE
110 120 130 140 150
DLLMWSIRQQ CEVGEELIDR WGSDSDDCFR DNEGRGQWVK GKELVKRQNN
160 170 180 190 200
NHFAHHTCNK SWRCGISTSK MYSRLECQDD TDECQVYILD AEGNPINVTV
210 220 230 240 250
DTVLHRDGVS MILKQKSTFT TRQIKAACLL IKDDKNNPES VTREHCLIDN
260 270 280 290 300
DIYDLSKNTW NCKFNRCIKR KVEHRVKKRP PTWRHNVRAK YTEGDTATKG
310 320 330 340 350
DLMHIQEELM YENDLLKMNI ELMHAHINKL NNMLHDLIVS VAKVDERLIG
360 370 380 390 400
NLMNNSVSST FLSDDTFLLM PCTNPPAHTS NCYNNSIYKE GRWVANTDSS
410 420 430 440 450
QCIDFSNYKE LAIDDDVEFW IPTIGNTTYH DSWKDASGWS FIAQQKSNLI
460 470 480 490 500
TTMENTKFGG VGTSLSDITS MAEGELAAKL TSFMFGHVVN FVIILIVILF
510
LYCMIRNRNR QY
Length:512
Mass (Da):58,566
Last modified:November 1, 1995 - v2
Checksum:iA3BC357846C7D2E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti406S → R in AAA72760 (PubMed:2644449).Curated1
Sequence conflicti414D → H (PubMed:2644449).Curated1
Sequence conflicti416Missing (PubMed:2644449).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25420 Genomic DNA. Translation: AAA72760.1.
L22858 Genomic DNA. Translation: AAA66758.2.
PIRiA72866.
RefSeqiNP_054158.1. NC_001623.1.

Genome annotation databases

GeneIDi1403961.
KEGGivg:1403961.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25420 Genomic DNA. Translation: AAA72760.1.
L22858 Genomic DNA. Translation: AAA66758.2.
PIRiA72866.
RefSeqiNP_054158.1. NC_001623.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DUZX-ray2.95A21-499[»]
ProteinModelPortaliP17501.
SMRiP17501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46361N.

Protein family/group databases

TCDBi1.G.15.1.1. the autographa californica nuclear polyhedrosis virus major envelope glycoprotein gp64 (gp64) family.

PTM databases

SwissPalmiP17501.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1403961.
KEGGivg:1403961.

Miscellaneous databases

EvolutionaryTraceiP17501.

Family and domain databases

InterProiIPR004955. Baculovirus_Gp64.
[Graphical view]
PfamiPF03273. Baculo_gp64. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUS_NPVAC
AccessioniPrimary (citable) accession number: P17501
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.