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Protein

Bleomycin resistance protein

Gene

ble

Organism
Streptoalloteichus hindustanus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Binding protein with a strong affinity to the bleomycin family of antibiotics.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Bleomycin resistance protein
Short name:
BRP
Alternative name(s):
Phleomycin resistance protein
Gene namesi
Name:ble
OrganismiStreptoalloteichus hindustanus
Taxonomic identifieri2017 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeStreptoalloteichus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 124124Bleomycin resistance proteinPRO_0000068563Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
124
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 1412Combined sources
Helixi16 – 2510Combined sources
Beta strandi30 – 345Combined sources
Beta strandi36 – 438Combined sources
Beta strandi46 – 527Combined sources
Turni56 – 583Combined sources
Helixi59 – 613Combined sources
Beta strandi63 – 697Combined sources
Helixi71 – 788Combined sources
Turni79 – 813Combined sources
Beta strandi82 – 843Combined sources
Turni86 – 883Combined sources
Beta strandi90 – 945Combined sources
Beta strandi98 – 1003Combined sources
Beta strandi103 – 1097Combined sources
Beta strandi115 – 1206Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BYLX-ray2.30A1-124[»]
1XRKX-ray1.50A/B1-124[»]
2ZHPX-ray1.60A/B1-124[»]
ProteinModelPortaliP17493.
SMRiP17493. Positions 1-121.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17493.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
InterProiIPR000335. Bleomycin-R.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
[Graphical view]
PRINTSiPR00311. BLEOMYCINRST.
SUPFAMiSSF54593. SSF54593. 1 hit.

Sequencei

Sequence statusi: Complete.

P17493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLTSAVPV LTARDVAGAV EFWTDRLGFS RDFVEDDFAG VVRDDVTLFI
60 70 80 90 100
SAVQDQVVPD NTLAWVWVRG LDELYAEWSE VVSTNFRDAS GPAMTEIGEQ
110 120
PWGREFALRD PAGNCVHFVA EEQD
Length:124
Mass (Da):13,796
Last modified:August 1, 1990 - v1
Checksum:i13CCB17F1CC14887
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52869 Genomic DNA. Translation: CAA37050.1.
PIRiS10304.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52869 Genomic DNA. Translation: CAA37050.1.
PIRiS10304.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BYLX-ray2.30A1-124[»]
1XRKX-ray1.50A/B1-124[»]
2ZHPX-ray1.60A/B1-124[»]
ProteinModelPortaliP17493.
SMRiP17493. Positions 1-121.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP17493.

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
InterProiIPR000335. Bleomycin-R.
IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
[Graphical view]
PRINTSiPR00311. BLEOMYCINRST.
SUPFAMiSSF54593. SSF54593. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance."
    Drocourt D., Calmels T., Reynes J.-P., Baron M., Tiraby G.
    Nucleic Acids Res. 18:4009-4009(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 31158 / E465-94.
  2. "Crystal structure and site-directed mutagenesis of a bleomycin resistance protein and their significance for drug sequestering."
    Dumas P., Bergdoll M., Cagnon C., Masson J.-M.
    EMBO J. 13:2483-2492(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
    Strain: ATCC 31158 / E465-94.

Entry informationi

Entry nameiBLE_STRHI
AccessioniPrimary (citable) accession number: P17493
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 11, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.